Benjamin Rodriguez-Garay

Retired researcher

Spirulina is an edible algae and has a wide range of pharmaceutical applications in addition to its nutritional value. Isolation and identification of several Spirulina species were conducted in the western part of Mexico especially in the state of Jalisco. The purification strategy consisted of five optimized processing steps: 1) washing and centr...

Simple methods to evaluate the viability and to preserve Agave tequilana Weber var. azul and A. angustifolia Haw pollen were established. Pollen viability was assessed by improving a growing media and evaluating three common pollen germination media components: sucrose, boric acid, and calcium ion. Optimal germination of pollen was obtained at 0.30...

The laser-induced fluorescence (LIF) was tested as a means to detect the effects of a pink-pigmented facultative methylotrophic bacteria (Methylobacterium) in blue agave plants, and compared with conventional techniques of leaf tissue analysis. A He–Ne laser emitting at 632.8nm was used as the excitation source. In vivo fluorescence emission spectr...

Plant regeneration through direct somatic embryogenesis of leaf blade explants from in vitro propagated plants of Agave victoria-reginae Moore, is described. Somatic embryogenesis was evident in a 6-week period on agarsolidified MS medium supplemented with L2 vitamins and 2,4-dichlorophenoxyacetic acid (1,4 M), and germination of somatic embryos wa...

Several methods of storing cotton pollen (Gossypium hirsutum L.) were evaluated. A successful pollen storage method that maintains fertility would enhance the crossing of breeding materials. Storing pollen at ultra-low temperatures in liquid nitrogen or at 5°C was not successful. No storage method maintained pollen fertility for more than 72 h. Cot...