Benjamin Pommerrenig

Benjamin Pommerrenig
Technische Universität Kaiserslautern | TUK · Division of Plant Physiology

PhD

About

53
Publications
12,120
Reads
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991
Citations
Introduction
Benjamin is interested in plant carbohydrate and nutrient transport and in the long-distance translocation of both via the phloem from source to sink tissues. He studies molecular and physiological responses of crop (sugar beet, rapeseed, cassava) and model plants (Arabidopsis, common plantain) to abiotic stress and nutrient deficiency and focusses on the role of transport proteins during such conditions.
Additional affiliations
November 2016 - present
Technische Universität Kaiserslautern
Position
  • Group Leader
October 2013 - October 2016
Leibniz Institute of Plant Genetics and Crop Plant Research
Position
  • PostDoc Position
Description
  • Studying the molecular and genetic backgrounds of nutrient-efficiency and boron transport in rapeseed
January 2008 - September 2013
Friedrich-Alexander-University of Erlangen-Nürnberg
Position
  • PostDoc Position
Description
  • Worked on molecular and physiological mechanisms of sugar transport and vascular trafficking in Arabidopsis, Plantago, and sugar beet
Education
January 2003 - December 2007

Publications

Publications (53)
Article
Boron (B) deficiency is a highly prominent nutrient disorder. While B‐efficient accessions have recently been identified in the highly B‐demanding crop oilseed rape, it remained unclear which physiological processes underlie B efficiency and which signalling pathways trigger an efficient B‐deficiency response. Here, we compared, under three differe...
Article
Full-text available
Background DNA methylation is thought to influence the expression of genes, especially in response to changing environmental conditions and developmental changes. Sugar beet (Beta vulgaris ssp. vulgaris), and other biennial or perennial plants are inevitably exposed to fluctuating temperatures throughout their lifecycle and might even require such...
Article
Overexpression of the vacuolar sugar transporter TST1 in Arabidopsis leads to higher seed lipid levels and higher total seed yield per plant. However, effects on fruit biomass have not been observed in crop plants like melon, strawberry, cotton, apple, or tomato with increased TST activity. Thus, it was unclear whether overexpression of TST in sele...
Preprint
Full-text available
Background DNA methylation is thought to influence the expression of genes, especially in response to changing environmental conditions and developmental changes. Sugar beet (Beta vulgaris ssp. vulgaris), and other biennial or perennial plants are inevitably exposed to fluctuating temperatures throughout their lifecycle and might even require such...
Preprint
Full-text available
As the major sugar-producing crop in Europe and North America, sugar beet ( Beta vulgaris ) taproots store sucrose at a concentration of about 20 %. While the TST sucrose transporter, which drives vacuolar sugar accumulation in the taproot, has already been identified, sugar transporters mediating sucrose uptake across the plasma membrane of taproo...
Article
Root growth and architecture is markedly influenced by both developmental and environmental cues. Sugars integrate different stimuli and are essential building blocks and signaling molecules for modulating the root system. Members from the SUGAR WILL EVENTUALLY BE EXPORTED TRANSPORTER (SWEET) family facilitate the transport of different sugars over...
Article
Full-text available
Sugar beet ( Beta vulgaris subsp. vulgaris ) is the exclusive source of sugar in the form of sucrose in temperate climate zones. Sugar beet is grown there as an annual crop from spring to autumn because of the damaging effect of freezing temperatures to taproot tissue. A collection of hybrid and non-hybrid sugar beet cultivars was tested for winter...
Preprint
Full-text available
Sugar beet ( Beta vulgaris subsp. vulgaris ) is the exclusive source of sugar in the form of sucrose in temperate climate zones. There, sugar beet is grown as an annual crop from spring to autumn because of the damaging effect of freezing temperatures to taproot tissue. Natural and breeded varieties display variance in the degree of tolerance to fr...
Article
Maltose, the major product of starch breakdown in Arabidopsis (Arabidopsis thaliana) leaves, exits the chloroplast via the maltose transporter MEX1. Consequently, mex1 loss-of-function plants exhibit substantial maltose accumulation, a starch-excess phenotype and a specific chlorotic phenotype during leaf development. Here, we investigated whether...
Article
Sugars are the main building blocks for carbohydrate storage, but also serve as signaling molecules and protective compounds during abiotic stress responses. Accordingly, sugar transport proteins fulfill multiple roles as they mediate long distance sugar allocation, but also shape the subcellular and tissue-specific carbohydrate profiles by balanci...
Article
Full-text available
Potassium (K) is essential for the processes critical for plant performance, including photosynthesis, carbon assimilation, and response to stress. K also influences translocation of sugars in the phloem and regulates sucrose metabolism. Several plant species synthesize polyols and transport these sugar alcohols from source to sink tissues. Limited...
Article
During their first year of growth, overwintering biennial plants transport sucrose through the phloem from photosynthetic source tissues to storage tissues. In their second year, they mobilize carbon from these storage tissues to fuel new growth and reproduction. However, both the mechanisms driving this shift and the link to reproductive growth re...
Article
Most cellular sucrose is present in the cytosol and vacuoles of plant cells; however, little is known about the effect of this sucrose compartmentation on plant properties. Here, we examined the effects of altered intracellular sucrose compartmentation in Arabidopsis thaliana leaves by heterologously expressing the sugar beet (Beta vulgaris) vacuol...
Article
Sugar transport proteins are crucial for coordinated allocation of sugars. In this review we summarize recent key findings of roles and regulations of sugar transporters in inter- and intracellular transport by focusing on applied approaches, demonstrating how sucrose transporter activity may alter source and sink dynamics and their identities. The...
Preprint
Full-text available
During vegetative growth, biennial sugar beets maintain a steep gradient between the shoot (source) and the sucrose-storing taproot (sink). To shift from vegetative to generative growth, they require a chilling phase, called vernalization. Here, we studied sugar beet sink-source dynamics upon cold temperature-induced vernalization and revealed a pr...
Preprint
Full-text available
Although we know that most of the cellular sucrose is present in the cytosol and vacuole, our knowledge on the impact of this sucrose compartmentation on plant properties is still fragmentary. Here we attempted to alter the intracellular sucrose compartmentation of Arabidopsis mesophyll cells by either, overexpression of the vacuolar sucrose loader...
Article
The ability of plants to withstand cold temperatures relies on their photosynthetic activity. Thus, the chloroplast is of utmost importance for cold acclimation and acquisition of freezing tolerance. During cold acclimation, the properties of the chloroplast change markedly. To provide the most comprehensive view of the protein repertoire of the ch...
Preprint
Full-text available
The ability of plants to cope with cold temperatures relies on their photosynthetic activity. This already demonstrates that the chloroplast is of utmost importance for cold acclimation and acquisition of freezing tolerance. During cold acclimation, the properties of the chloroplast change markedly. To provide the most comprehensive view of the pro...
Article
Full-text available
Nodulin26‐like‐intrinsic‐proteins (NIPs) play essential roles in transporting the nutrients, silicon and boron, in seed plants, but the evolutionary origin of this transport function and the co‐permeability to toxic arsenic remains enigmatic. Horizontal gene transfer of a yet uncharacterized bacterial AqpN‐aquaporin group was the starting‐point for...
Article
Full-text available
Vascular tissues essentially regulate water, nutrient, photo-assimilate, and phytohormone logistics throughout the plant body. Boron (B) is crucial for the development of the vascular tissue in many dicotyledonous plant taxa and B deficiency particularly affects the integrity of phloem and xylem vessels, and, therefore, functionality of long-distan...
Article
Full-text available
The sophisticated uptake and translocation regulation of the essential element boron (B) in plants is ensured by two transmembrane transporter families: the Nodulin26‐like Intrinsic Protein (NIP) and BOR transporter family. Though the agriculturally important crop Brassica napus is highly sensitive to B deficiency and NIPs and BORs have been sugges...
Article
Sucrose is one of the most important types of sugars in plants serving inter alia as a long-distance transport molecule, a carbon and energy storage compound, an osmotically active solute, and fuel for many anabolic reactions. Sucrose biosynthesis and degradation pathways are well known; however, regulation of sucrose intracellular distribution is...
Article
Full-text available
Boron (B) is an essential micronutrient for seed plants. Information on B-efficiency mechanisms and B-efficient crop and model plant genotypes is very scarce. Studies evaluating the basis and consequences of B-deficiency and B-efficiency are limited by the facts that B occurs as a trace contaminant essentially everywhere, its bioavailability is dif...
Article
The sessile lifestyle of higher plants is accompanied by their remarkable ability to tolerate unfavourable environmental conditions. This is, because during evolution plants developed a sophisticated repertoire of molecular and metabolic reactions to cope with changing biotic and abiotic challenges. In particular, the abiotic factors light intensit...
Article
Full-text available
• Sugar beet (Beta vulgaris L.) is one of the most important sugar producing plants worldwide and provides about one third of the sugar consumed by humans. Here we report on the molecular characterization of the BvSUT1 gene and on the func- tional characterization of the encoded transporter. • In contrast to the recently identified, tonoplast-local...
Article
Full-text available
The Yang or Methionine (Met) Cycle is a series of reactions catalyzing the recycling of the sulfur (S) compound 5'-methylthioadenosine (MTA) to Met. MTA is produced as a byproduct in ethylene, nicotianamine and polyamine biosynthesis. Whether the Met Cycle preferentially fuels one of these pathways in a S-dependent manner remained unclear so far. W...
Article
Full-text available
Metalloids are a group of physiologically important elements ranging from the essential to the highly toxic. Arsenic, antimony, germanium, and tellurium are highly toxic to plants themselves and to consumers of metalloid-contaminated plants. Boron, silicon, and selenium fulfill essential or beneficial functions in plants. However, when present at h...
Article
Full-text available
Aquaporins (AQPs) are essential channel proteins that regulate plant water homeostasis and the uptake and distribution of uncharged solutes such as metalloids, urea, ammonia and carbon dioxide. Despite their importance as crop plants, little is known about AQP gene and protein function in cabbage (Brassica oleracea) and other Brassica species. The...
Article
Full-text available
Sugar beet provides around one third of the sugar consumed worldwide and serves as a significant source of bioenergy in the form of ethanol. Sucrose accounts for up to 18% of plant fresh weight in sugar beet. Most of the sucrose is concentrated in the taproot, where it accumulates in the vacuoles. Despite 30 years of intensive research, the transpo...
Data
Young seedlings (a) and fully developed rosettes (b) of plants grown with supplementation of various amounts of biotin.
Data
Seedlings (a) and fully developed rosettes (b) of the same plants germinated on biotin-containing (1mm) agar medium for 10 days, transferred to soils and supplemented with various amounts of biotin.
Article
Full-text available
The Arabidopsis SUC5 protein represents a classical sucrose/H(+) symporter. Functional analyses had revealed that SUC5 could also transport biotin, an essential cofactor for fatty acid synthesis. However, evidence for a dual role in the transport of the structurally unrelated compounds sucrose and biotin in plants was lacking. Here we show that SUC...
Article
Full-text available
The 5-methylthioadenosine (MTA) or Yang cycle is a set of reactions that recycle MTA to Met. In plants, MTA is a byproduct of polyamine, ethylene, and nicotianamine biosynthesis. Vascular transcriptome analyses revealed phloem-specific expression of the Yang cycle gene 5-METHYLTHIORIBOSE KINASE1 (MTK1) in Plantago major and Arabidopsis thaliana. As...
Thesis
Full-text available
Die Leitbündel Höherer Pflanzen stellen die wesentlichen Gewebe zur Verteilung von Assimilaten, zum Transport von Wasser und zur Übermittlung von Informationen innerhalb und zwischen den einzelnen pflanzlichen Organen dar. Die Zellen des vaskulären Gewebes unterscheiden sich von denen des Mesophylls nicht nur in ihrer Gestalt und Funktion, sondern...
Article
Full-text available
Several plant families generate polyols, the reduced form of monosaccharides, as one of their primary photosynthetic products. Together with sucrose (Suc) or raffinose, these polyols are used for long-distance allocation of photosynthetically fixed carbon in the phloem. Many species from these families accumulate these polyols under salt or drought...
Article
Full-text available
The vascular tissue of higher plants consists of specialized cells that differ from all other cells with respect to their shape and size, their organellar composition, their extracellular matrix, the type of their plasmodesmata, and their physiological functions. Intact and pure vascular tissue can be isolated easily and rapidly from leaf blades of...
Article
K+ channels control K+ homeostasis and the membrane potential in the sieve element/companion cell complexes. K+ channels from Arabidopsis phloem cells expressing green fluorescent protein (GFP) under the control of the AtSUC2 promoter were analysed using the patch-clamp technique and quantitative RT-PCR. Single green fluorescent protoplasts were se...

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Projects

Projects (3)
Project
Balanced nutrition is crucial for plant development and stress tolerance. Imbalance of the nutrient supply might result in physiological and metabolic disorders affecting proper growth and adjustment to environmental changes. We are interested in molecular and physiological processes altered during impaired nutrient supply. In particlar we explore connections between carbohydate metabolism, sink to source allocation of sugars, and alted supply of macro and micronutrients. We are also interested in the discovery of factors responsible for nutrient efficiency and stress tolerance.
Project
We aim to improve the cold hardiness of sugar beet (Beta vulgaris) plants and to establish the bi-annual cultivation of elite sugar beet varieties in temperate climate zones.
Project
Metabolic engineering of carbon pathways to enhance yield of root and tuber crops