Benedikt Weber

Benedikt Weber
Medical University of Vienna | MedUni Vienna · Skin and Endothelium Research Division SERD

About

133
Publications
12,534
Reads
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2,225
Citations
Citations since 2016
66 Research Items
1569 Citations
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300

Publications

Publications (133)
Article
Full-text available
Atherosclerotic plaques are cholesterol-induced inflammatory niches accumulating in the vascular sub-endothelial space. Cellular and extracellular composition of human plaques is maneuvered by local inflammation that leads to alterations in the original vascular microenvironment and to the recruitment of an invading fibrous layer (fibroatharoma). I...
Article
Full-text available
Human mesenchymal stromal cell (hMSC) secretomes have shown to influence the microenvironment upon injury, promoting cytoprotection, angiogenesis, and tissue repair. The angiogenic potential is of particular interest for the treatment of ischemic diseases. Interestingly, hMSC secretomes isolated from different tissue sources have shown dissimilarit...
Article
Full-text available
An ideal cell source for human therapeutic and disease modeling applications should be easily accessible and possess unlimited differentiation and expansion potential. Human induced pluripotent stem cells (hiPSCs) derived from peripheral blood mononuclear cells (PBMCs) represent a promising source given their ease of harvest and their pluripotent n...
Article
Full-text available
Pluripotent stem cells (PSCs) hold great potential for novel therapeutic approaches to regenerate or replace functionally impaired tissues. Since the introduction of the induced pluripotent stem cell technology in 2006, the number of scientific publications on this topic has constantly been increasing. However, so far no therapy based on PSCs has f...
Article
Full-text available
Zusammenfassung Das Ulcus cruris venosum entsteht im Rahmen einer fortgeschrittenen chronisch-venösen Insuffizienz und stellt die häufigste Entität chronischer Ulzerationen der unteren Extremität dar. Da die Prävalenz im Alter deutlich ansteigt, stellen venöse Ulzera in einer alternden Gesellschaft ein zunehmend relevantes Gesundheitsproblem dar, w...
Article
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Background Endovenous thermal and non-thermal therapeutic approaches have become standard of care for the treatment of venous insufficiency. However, comparative studies on its use in the population of venous leg ulcer patients are scarce. Methods The present study aimed at a comparison of the efficacy of endovenous laser ablation (EVLA) and ultra...
Article
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In an aging society, chronic ulcers pose an increasingly relevant healthcare issue associated with significant morbidity and an increasing financial burden. Hence, there is an unmet medical need for novel, cost-effective therapies that improve healing of chronic cutaneous wounds. This prospective, randomized, open-label, phase I trial investigated...
Article
Background: Calciphylaxis and the arteriolosclerotic ulcer of Martorell (ASUM) represent two entities of cutaneous calcific arteriolopathies. Their differential diagnosis can be challenging, given similarities in their clinical and histological presentation. Calcification patterns have been proposed as a possible discriminative histological criter...
Article
Full-text available
Herein, we report a case of a new-onset Raynaud’s phenomenon (RP), which occurred in an otherwise healthy 31-year-old Caucasian woman, who lacked any known risk factors and associations with possible causes for secondary RP. However, 2 weeks prior to the development of RP, the patient had received her first injection of the COVID-19 vaccine contain...
Article
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Background The aim of this retrospective study was to compare the efficacy and safety of different phototherapeutic modalities in the treatment of cutaneous lichen planus (LP). Methods We retrospectively analyzed the chart data of 53 patients with generalized LP who had been subjected to narrowband UVB (NB-UVB) or photochemotherapy (PUVA) between...
Article
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Cell-cell junctions of blood endothelial cells are critical barriers in inflammatory diseases. Endothelial tight junctions (TJs) control barrier function, and the cytoplasmic adaptor protein cingulin connects TJs to signalling pathways. However, local events at TJs during inflammation are largely unknown. In this study, we investigate the local res...
Article
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Background Arteriolosclerotic ulcers of Martorell are histologically characterized by hyaline arteriolosclerosis resulting in concentric occlusion of the arteriolar lumina. Although several authors have previously reported on hyaline changes in hypertensive arteriolopathies, so far, little information is available on the molecular composition of hy...
Article
Clinical differential diagnosis of arteriolosclerotic ulcers of Martorell is challenging due to the lack of clearly affirmative instrument-based diagnostic criteria. The aim of this study was to develop vascular histomorphological diagnostic criteria differentiating Martorell ulcers from other types of leg ulcers. The histomorphology of patients di...
Preprint
Full-text available
Atherosclerosis is an arterial disease characterized by intravascular plaques. Disease hallmarks are vessel stenosis and hyperplasia, eventually escalating into plaque rupture and acute clinical presentations. Innate immune cells and local variations in hemodynamics are core players in the pathology, but their mutual relationship has never been inv...
Article
Full-text available
Drug‐induced photosensitivity, the development of phototoxic or photoallergic reactions due to pharmaceuticals and subsequent exposure to ultraviolet or visible light, is an adverse effect of growing interest. This is illustrated by the broad spectrum of recent investigations on the topic, ranging from molecular mechanisms and culprit drugs through...
Article
Full-text available
Zusammenfassung Medikamenten‐induzierte Photosensibilität, also phototoxische oder photoallergische Reaktionen durch Pharmazeutika und nachfolgende Exposition gegenüber ultraviolettem oder sichtbarem Licht, ist eine unerwünschte Wirkung, die zunehmend an Interesse gewinnt. Dies zeigt sich am breiten Spektrum aktueller Untersuchungen, die von moleku...
Chapter
Vascular in vitro tissue engineering has seen a constant evolution from first in vitro engineered constructs to fully functional, growing replacements in large animal in vivo models. The rise of the induced pluripotent stem cells (iPSCs) technology had a major impact on the entire field providing a promising cell source for tissue engineering and r...
Preprint
Full-text available
Background: Pluripotent stem cells (PSCs) hold great potential for novel therapeutic approaches to regenerate or replace functionally impaired tissues. Since the introduction of the induced pluripotent stem cell technology in 2006, the number of scientific publications on this topic has constantly been increasing. However, so far no therapy based o...
Article
The arteriolosclerotic ulcer of Martorell (ASUM) represents an important differential diagnosis of leg ulcers. According to Martorell, this condition is characterized by several clinical features including a typical ulcer location on the lower leg(s), absence of relevant peripheral artery occlusive disease or venous insufficiency, disproportionate...
Article
Background: Recent experimental data suggest a role for apremilast in the treatment of alopecia areata. Small clinical studies have so far provided contradictory results. Objectives: We retrospectively evaluated the efficacy and safety of apremilast in five cases of extensive and treatment-resistant alopecia areata. Materials and methods: Apre...
Article
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Background The donkey pericardium is considered a good candidate to manufacture percutaneous heart valves based upon its thinness, low cellularity and undulating collagen bundles and laminates. Decellularization represents an avenue worth exploring, should its superiority to glutaraldehyde-treated pericardium be demonstrated. Materials and methods...
Article
Full-text available
Background: Drug-induced photosensitivity refers to the development of cutaneous adverse events due to interaction between a pharmaceutical compound and sunlight. Although photosensitivity is a very commonly listed side effect of systemic drugs reliable data on its actual incidence are lacking so far. Objectives: A possible approach to evaluate...
Article
Full-text available
Background: Narrowband UVB phototherapy (NB UVB) is a mainstay in the treatment of numerous inflammatory dermatoses. Whereas a wealth of studies has shown that NB UVB treatment increases 25-hydroxyvitamin D3 (25(OH)D) levels, only sparse and controversial data exist on its effect on serum folate and cobalamin. Objectives: To determine whether ex...
Article
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The ovine developmental model represents the standard in vivo model for studies involving maternofetal physiology, amniotic fluid (AF) research, and fetal cell therapy prior to human clinical use. Although being close to the human fetal anatomy, 2 separate extraembryonic fluid compartments remain during gestation, known as the amnion and the allant...
Article
Background: A hallmark of Euphorbia myrsinites (EM), a member of the widespread perennial Euphorbia species, is the extrusion of a poisonous, latex-like sap irritant to the skin and eye after contact. The exact mechanisms underlying these effects have not been unraveled so far. Objectives: The aims of the study were to allocate EM sap-induced ph...
Patent
The invention provides a method for the generation of a layered cellular 3 D microtissue aggregate, comprising the steps of contacting myeloid cells with a protein kinase C agonist, yielding primed myeloid cells; incubating the primed myeloid cells in the presence of LDL in a confined volume, particularly in a hanging drop culture; yielding a 3 D c...
Article
Full-text available
Introduction Finding new markers to assess prognosis of melanoma without the necessity to perform a surgical interventions is an important goal in melanoma research. The current study aimed to assess the correlation of clinical course and prognosis of primary and metastatic melanoma with expression of VEGF family and their receptors. Methods A ngT...
Data
Staining protocols using BOND-III fully automated IHC stainer. (DOCX)
Data
Loading plot showing the relationship between original variables and the first two components extracted by using principal component analysis (PCA). The angle between vectors is proportional to the degree of correlation between variables, while the length of vectors is proportional to the correlation between variables and PCA components. (TIF)
Data
Distribution of study scores across demographics and clinical characteristics of patients with metastasis. (DOCX)
Data
Univariate analysis of the association between categorized scores and overall and disease-free patients’ survival. (DOCX)
Data
Red staining was performed a cut from TMA-block stained using anti-VEGF-D antibody (R&D systems). The stained slide was scanned by panoramic Digital Slide Scanner (3DHISTECH). The unsuitable cores for analysis were marked as ’Missing data’ and excluded. (TIF)
Data
Pearson’s correlations between each pair of scores. (DOCX)
Data
Distribution of study scores across demographics and clinical characteristics of patients with a diagnosis of MM. (DOCX)
Article
Full-text available
Despite advances in understanding the underlying mechanisms of flap necrosis and improvement in surgical techniques, skin flap necrosis after reconstructive surgery remains a crucial issue. We investigated the efficacy of electroporation-mediated IL-10 gene transfer to random skin flap with an aim to accelerate wound healing and improve skin flap s...
Article
Statement of significance: The concept of living tissue engineered, self-repairing, autologous cardiovascular replacements, was proposed alternatively to existing synthetic/xenogeneic prostheses. Recent studies in animal models demonstrate faster in vivo recellularization after grafts pre-seeding with cells prior implantation. Pre-seeded cells hol...
Article
Background: Replacement of the tricuspid valve is uncommon. Prostheses specifically designed for the this position are not available. Bovine, porcine as well as mechanical valve prostheses are currently used, however, the most suitable prosthesis type has not been defined. Methods: We retrospectively analyzed all consecutive patients who underwe...
Chapter
Heart valve disease is a significant cause of morbidity and mortality worldwide. Its spectrum ranges from congenital disorders, where heart valves are either absent or malformed, to dysfunctional heart valves due to infection or age-related structural changes. Current options of surgical heart valve replacement are associated with several disadvant...
Article
Autologous cell-based therapies got a step closer to reality with the introduction of induced pluripotent stem cells. Fetal stem cells, such as amniotic fluid and membrane mesenchymal stem cells, represent a unique type of undifferentiated cells with promise in tissue engineering and for reprogramming into iPSC for future pediatric interventions an...
Article
Fetal stem cells are a unique type of adult stem cells that have been suggested to be broadly multipotent with some features of pluripotency. Their clinical potential has been documented but their upgrade to full pluripotency could open up a wide range of cell-based therapies particularly suited for pediatric tissue engineering, longitudinal studie...
Article
Amniotic fluid represents an abundant source of multipotent stem cells, referred as broadly mul-tipotent given their differentiation potential and expression of pluripotency-related genes. How-ever, the origin of this broadly multipotent cellular fraction is not fully understood. Several sources have been proposed so far, including embryonic and ex...
Article
Biodegradable scaffold matrixes form the basis of any in vitro tissue engineering approach by acting as a temporary matrix for cell proliferation and extracellular matrix deposition until the scaffold is replaced by neo-tissue. In this context several synthetic polymers have been investigated, however a concise systematic comparative analyses is mi...
Article
Background: Regular use of sunbed exposure has been reported to increase 25-hydroxyvitamin-D3 [25(OH)D] serum levels. However, the influence of sunbeds compliant with the recent European Union standard EN-60335-2-27 on 25(OH)D serum levels is unknown. Objective: We investigated the impact of standard sunbed use compliant with the European Union...
Chapter
Heart valve disease is a leading cause of mortality worldwide. New techniques and prostheses for valvular replacement have been recently introduced into the clinical routine, increasing the range of treatment opportunities for the patients. Despite the efforts, today's heart valve artificial prostheses are still associated with increased risk of th...
Article
Tissue engineered scaffolds have emerged as a promising solution for heart valve replacement because of their potential for regeneration. However, traditional heart valve tissue engineering has relied on resource-intensive, cell-based manufacturing, which increases cost and hinders clinical translation. To overcome these limitations, in situ tissue...
Article
Background: Autosomal recessive congenital ichthyosis (ARCI) is a genetically heterogeneous group of rare Mendelian skin disorders characterized by cornification and differentiation defects of keratinocytes. Mutations in nine genes including PNPLA1 are known to cause non-syndromic forms of ARCI. To date, only ten distinct pathogenic mutations in P...
Article
To date, clinical success of cardiac cell-therapies remains limited. To enhance the cardioreparative properties of stem cells, the concept of lineage-specification through cardiopoietic-guidance has been recently suggested. However, so far, only results from murine studies and from a clinical pilot-trial in chronic heart-failure (CHF) are available...
Article
Background: Cutaneous (CLP) and oral lichen planus (OLP) as the main subtypes of lichen planus (LP) present with different clinical manifestation and disease course, although their histopathologic features such as the band-like lymphocyte infiltrate and keratinocyte apoptosis are similar. So far, the underlying cellular and molecular mechanisms re...
Article
Introduction: Chronic insufficiency of lower extremity venous valves represents a frequent structural disorder of the vascular system being responsible for a substantial global disease load. While in the field of superficial valve insufficiency surgical as well as endoluminal interventions represent good therapeutic options with high rates of comp...
Article
Aims: This study set out to assess indications, feasibility, complications, and clinical outcome of percutaneous transcatheter device closure of atrial septal defects (ASDs) in infants with a bodyweight below 10 kg. Methods and results: Retrospective single center chart and echocardiography review study from 8/2005-12/2013. Twenty-eight children...
Article
Background: Port-wine stains (PWS) are relatively common and often cause cosmetic and psychological concerns. The pulsed dye laser is currently the treatment of choice for PWS. Objective: To assess the effectiveness of the pulsed sequential dual wavelength 595 and 1064 nm laser as first-line treatment for PWS and to identify prognostic factors f...
Article
Background: Heterotopic heart transplantation (HHT) in rodent animal models represents an important technique enabling studies on organ transplantation immunology and pharmaceutical development. Recent investigations used nonworking HHT designs, with the left ventricle (LV) bypassed in the anastomosis system. In spite of their principal success, t...
Article
Full-text available
In the effort of improving treatment for cardiovascular disease (CVD), scientists struggle with the lack of the regenerative capacities of finally differentiated cardiovascular tissues. In this context, the advancements in regenerative medicine contributed to the development of cell-based therapies as well as macro- and micro-scale tissue-engineeri...
Article
BACKGROUND AND AIM OF THE STUDY: Bioengineered living autologous valves with remodeling and growth capacity represent a promising concept for future cardiac and venous valve repair. A meticulous understanding of the mechanisms involved in recellularization and remodeling is essential for the safe and efficient clinical translation of this technolog...
Article
Valvular heart disease remains to be a major cause of death worldwide with increasing prevalence, mortality and morbidity. Current heart valve replacements are associated with several limitations due to their nonviable nature. In this regard, heart valve tissue engineering has shown to represent a promising concept in order to overcome these limita...
Article
Amniotic fluid stem cells (AFSC) represent an attractive potential cell source for fetal and pediatric cell-based therapies. However, upgrading them to pluripotency confers refractoriness towards senescence, higher proliferation rate and unlimited differentiation potential. AFSC were observed to rapidly and efficiently reacquire pluripotency which...
Article
Full-text available
Abstract Introduction: A major challenge associated with heart valve tissue engineering is the in vitro creation of mature tissue structures compliant with native valve functionality. Various cell types have been investigated for heart valve tissue engineering. In addition to prenatal, umbilical cord- and vascular-derived cells, mesenchymal stem ce...
Article
Full-text available
Restless legs syndrome (RLS) is characterised by an uncontrollable urge to move, in particular the lower limbs, often accompanied by discomfort or a painful sensation that occurs typically at night. It is categorized under the ICD-Classification of extrapyramidal and movement disorders. As patients often suffer from insomnia due to the involuntary...