Benayache Samir

• Professor
• Head of Department at University Frères Mentouri Constantine 1

This study was aimed at determining the phenolic and volatile bioac-tive components of Senecio massaicus extracts. According to GC-MS results, the main constituents of the petroleum ether extract were 2-(2, 2, 6-trimethylcyclohex-1-enyl) cyclopropane carboxylic acid, methyl ester, (-) caryophyllene oxide, 1-methoxy-3, 5-dimethyl cyclo-hexene, and d...

In this study, we investigated the volatile composition of the Algerian medicinal plant Calendula arvensis, as well as the antioxidant evaluation, total phenolics and flavonoids contents, and synergistic antibacterial effect of its ethanolic extract (CAEE) with chemical antibiotics against some bacterial strains. The results of the GC-MS and GC-FID...

This work examined the volatile profile, mineral, and water contents of the aromatic species Cedrus atlantica gathered in the Algerian Aurès zone during the summer. The presence of fifty-seven (57) compounds in C. atlantica oil was revealed by GC-MS and GC-FID analyses, with the major compounds being caryophyllene oxide (35.1%), humulene-2-epoxide...

Seven components from the methanol extract of the aerial part of the endemic species Helianthemum confertum were isolated and identified for the first time. Investigating this species and its separated components chemical make-up and radical scavenging capacity, was the main goal. Using an online HPLC-ABTS˙⁺ test, ORAC, and TEAC assays, the free ra...

Fagonia longispina (Zygophyllaceae family) has long been employed in traditional practices for combating cancer, with scientific research yielding insights into its biological impact. This study aimed to validate the potential of this plant by evaluating the antioxidant, antiproliferative, and antibacterial properties of the n-butanol, ethyl acetat...

Background Non-alcoholic Fatty liver disease (NAFLD) is becoming a major global health burden in the world. Cynara cardunculus is an edible plant growing wild in the North of Algeria. Its potential as a source of health-promoting compounds is still underexplored. Objectives This study aimed to explore the preventive effect of Cynara cardunculus (C...

Objective(s) The current study was conducted to assess the protective mechanisms of n-BuOH fraction from the aerial part of Genista cephontala (BEGC) on APAP-induced liver injury compared to necrostatine-1 (Nec-1). Materials and Methods A model of APAP-induced hepatotoxicity was created in male rats by injecting a single dose; 1000 mg/kg APAP, the...

In this study, for the first time, we analyzed the chemical composition of essential oils (EOs) steam‐distilled from the flowers and leaves of Perralderia coronopifolia by GC‐FID/MS. The objective was to explore new anticancer and antioxidant bioactive substances and understand their mechanisms of action through the use of plant‐derived natural pro...

Artemisia herba-alba and Artemisia campestris were harvested from the Algerian Aurès area. Isolation of their essential oils was performed using steam distillation method, and the volatile composition was characterized using GC-MS and GC-FID techniques. The results revealed that the dominant constituents of A. herba-alba oil were camphor (35.4%), c...

Hammada articulata is a plant widely used by the locals of the Algerian Sahara for multiple medicinal purposes. However, little was known about its chemical composition and the mechanisms of its bioactivity. For this purpose, the derived extracts [chloroform (CHCl3), ethyl acetate (EtOAc) and n-butanol (n-BuOH)] of the 80% ethanol extract of its ae...

The diversity of extractable compounds provides a potentially rich source of high-value products. The corrosion inhibition performances of seven Thymus algeriensis extracts on mild steel in 0.5 M HCl were investigated using the gravimetric method, electrochemical measurements, and surface analysis. The results indicate that the addition of all the...

Since antiquity, Centaurea species have been used in folk medicine to treat several diseases owing to their potential biological activities that distinguish this genus such as antioxidant, anticancer, and anti-inflammatory effect. The current study aimed to investigate the possible neuroprotective effects of the n-butanol extract of Centaurea maroc...

Volutaria lippii (L.) Cass. ex Maire, syn. Centaurea lippii (L.), (Asteraceae) is a plant from the central region of Algeria, considerably distributed in all Mediterranean areas. Herein, the antioxidant activity of the three derived fractions [chloroform (CHCl3), ethyl acetate (EtOAc) and n-butanol (n-BuOH)] of the 70% methanol extract of the aeria...

The essential oil of the aerial parts of Helianthemum ruficomumwas obtained by steam distillation andanalyzed by GC-FID and GC-MS. 59components were identified corresponding to 98.3% of the total oil. Among the identified constituents, oxygenated compounds represented 56.3% and hydrocarbons (43%).The major components were palmitic acid (9.1%), hene...

This work aimed to investigate the anti-inflammatory and the antioxidant activities of the hydroalcolic extract (HAEs) from aerial parts (stems and leaves separately) of an Algerianmedicinal plantCytisustriflorusL'Hérit. The anti-inflammatory activity is evaluated by the carrageenan induced mice hind paw edema model. Oral administration of HAEs tes...

The objective of this research was to investigate the chemical composition of the essential oil of Hammada articulata obtained by steam distillation and to investigate its antioxidant and anticorrosive activities. Gas chromatography–mass spectrometry analysis of this oil resulted in the identification of 46 compounds, which accounted for 97.0% of t...

The present study was aimed to investigate the essential oil composition, antioxidant and antibacterial evaluation of an Algerian Cistus albidus L. from the Aures region. Analyzes of oil was carried out using GC-MS and GC-FID techniques. The results revealed that the main compounds were respectively 15,16-dinorlabd-8(20)-en-13-one, methacrylic acid...

The present study, an Algerian Saharan species P. inuloides aerial part was investigated for its volatile composition, antioxidant and antibacterial capacity. The EO composition was characterized using GC-MS technique. Moreover, The CHCl 3 , AcOEt and n-BuOH extracts were tested for their antioxidant and antibacterial potential. The results reveale...

Abstract Doxorubicin (DOX) is a potent anticancer drug; its use has been limited by its hepatotoxicity, which is due to free radicals generation. This work aims to investigate whether the n-butanol soluble part of the 70% methanol extract of the aerial parts of Moricandia arvensis, alleviates doxorubicin-induced hepatotoxicity. According to the lit...

The Cistaceae is a large family of perennial herbaceous plants consisting of 8 genera including the genus Cistus and around 175 species. It is frequently found distributed in many parts of the world including the Aures area. C. albidus is a small evergreen shrub distributed especially in the arid places. In fact, many biological properties have bee...

The present study was to investigate the essential oil composition and the antioxidant properties of P. inuloides collected from the southwest of Algeria. The volatile composition was characterized using GC-MS technique, and antioxidant capacity was estimated by DPPH and β-carotene assays. The results revealed that the main compounds were δ-cadinen...

L'Algérie, de sa position géographique, présente une large gamme d'étages bioclimatiques, induisant une biodiversité de plantes utilisées comme condiments, aliments naturels et pour des buts thérapeutiques [1]. Elle possède une flore représentée par 4125 plantes vasculaire inventoriées, réparties en 123 familles botaniques [2]. Cette richesse fait...

Algerian Aures area is well known for its diversity in medicinal and aromatic species those have used in different forms and various ways. The genus Artemisia (Asteraceae) comprises around 500 species, mostly found in arid and semi-arid regions. This genus is known by its richness in essential oils. The aime of this work was to investigate and enri...

The present work was conducted to evaluate the quantitative analysis of aerial parts of Serratula cichoracea (DC.) and to evaluate their potential antioxidant, anti-alzheimer and antidiabetic properties in vitro. The chloroform, ethyl acetate and n-butanol extracts of aerial parts of Serratula cichoracea (DC.) were subjected to quantitative determi...

Background Many studies have used rotenone (ROT) to create an experimental animal model of Parkinson's disease (PD) because of its ability to induce similar behavioral and motor deficits. PD is the most common age-related motoric neurodegenerative disorder. Neuroinflammation and apoptosis play an important role in the pathogenesis of this disease....

The essential oil of the fresh aerial parts of Centaurea calitrapa Desf. (Asteraceae), synonym: Calcitrapa calcitrapa (L.) Hill (1768) (L.), was obtained by steam distillation and analyzed by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Seventeen components were identified corresponding to 94.7 % of the total oil. T...

Background The aim of the present study is to evaluate the protective effect of n-BuOH fraction of Genista vepres Pomel and Vitamin E against Isoniazid and Rifampicin (INH-RIF)-induced liver injury. Methods: Male Wistar Albino rats were dividing into eight equal groups treated with plant fraction (50 mg/kg, 100 mg/kg), vitamin E (100 mg/kg) and INH...

The composition of the essential oil obtained from the dried aerial parts of Senecio massaicus was analyzed by GC/MS. Twenty-two components have been identified and represented 97.41 % of the total oil composition. The major constituents of the essential oil were m-cymene (30.58 %), n-hexadecanoic acid (14.88 %) and docosane-11-decyl (10.43 %). Fou...

Background Long-term administration of valproic acid (VPA) is known to promote renal tubular injury mediated by increase in renal oxidative stress. Recent evidence indicates that natural antioxidants are alternative to attenuate oxidative stress and kidney damage. Objective This study was performed to investigate the protective effects of n-butano...

A new flavonoid, 5,7,2′,4′,5′-pentahydroxyflavone 3-O-β-D-galactopyranoside (12) and twelve known derivatives: an aryltetralin-lignan (3), seven flavonoids (4-5, 7-10, 13) and four phenolic acids (1-2, 6, 11) have been isolated from the aerial parts of Helianthemum getulum Pomel. (Cistaceae family) an endemic species to the septentrional Sahara tha...

The aim of the present study is to evaluate the total phenolic contents, antioxidant and anti-proliferative activities of the n-BuOH extract of Centaurea maroccana (BECM). The total phenolic and flavonoid of the butanolic extract of the plant were assessed by using Folin-Ciocalteau and aluminium chloride colorimetric assays, respectively, and the q...

Various parts of Crataegus oxyacantha (hawthorn) plant have been used in traditional medicine in many countries including Algeria. In this study, antioxidant (GOR, ABTS, FRAP, CUPRAC and phenanthroline), photoprotective, and inhibition ability of n-butanol and ethyl acetate extracts from leaves and fruits of C. oxyacantha against Alzheimer’s diseas...

Solid-Liquid Extraction (SLE) using solvent of different polarities (CHCl3, EtOAc, and n-BuOH) has been applied to leaves and fruits from (Crataegus oxyacantha L.), a deciduous shrub with an expected rich phytochemical profile. The total polyphenols content and the radical scavenging activity of each extract were evaluated. These extracts were anal...

Protozoan pathogens that cause neglected tropical diseases are a major public health concern in tropical and developing countries. In the course of our ongoing search for new lead compounds as potential antiprotozoal agents, this study aims to perform a bio-guided fractionation of Pituranthos battandieri, using an in vitro assay against Leishmania...

Limonium duriusculum is used in folk medicine to treat inflammatory disorders and has gained attention due to its richness in apigenin. The present investigation was performed to evaluate and confirm its anti-inflammatory properties, in cell lines and animal models. The potential anti-inflammatory properties of n-butanol (n-BuOH) extract of L. duri...

Background: Pistacia atlantica Desf. (Anacardiaceae) has various applications for dietetic and medicinal purposes. Objective: The aim of the present study was to evaluate antioxidant, antiproliferative and anticholinesterase activities of different extracts from leaf and stem of Pistacia atlantica Desf. Methods: The antioxidant activity was perfo...

The structural, electronic, and thermal properties of the BeSiAs2 and MgSiAs2 chalcopyrite compounds have been investigated using the full-potential linearized augmented plane wave (FP-LAPW) method. The exchange-correlation part of the potential is treated within the Wu and Cohen generalized gradient approximation (WC-GGA). Moreover, Tran and Blaha...

The present study was aimed to investigate the essential oil composition and the antioxidant properties of an Algerian Cistus albidus L. from the Aures region. The essential oil was extracted by steam distillation technique and analyzed by GC-MS and GC-FID methods and the antioxidant evaluation was determined by the DPPH radical scavenging assay. T...

Three undescribed coumarin derivatives, pituranthosin A–C (1-3), along with nineteen known compounds were isolated and identified from the chloroform and ethyl acetate fractions of the hydroalcoholic extract of Deverra battandieri (Apiaceae). The structures of the compounds were established by interpretation of their spectral data using 1D-NMR (¹H,...

This study was aimed to determine the chemical composition of essential oils obtained from aerial parts of Thymus algeriensis Boiss. & Reut. and Marrubium vulgare L. collected in the Aures region of Algeria. Essential oils were extracted by steam distillation method and analyzed using GC-MS and GC-FID techniques. Thirty-five (35) and twenty-nine (2...

Background: This study was aimed to evaluate the protective effects of n-butanol extract of Chrysanthemum fontanesii against oxidative stress induced by sodium valproate (VPA) in brain of female mice in comparison to vitamin E (Vit E). Method: Mice were randomly divided into 5 groups (n: 8) and treated daily for 12 days. They received VPA (300 mg/...

Background In Algerian traditional medicine, Centaurea species are well known in traditherapy. The Centaurea africana has been used in folk medicine for the treatment of several inflammatory disorders. Objective This study aims to examine the antioxidant, anti-inflammatory and anti-proliferative potential of both nButanol (BECA) and ethyl acetate...

The present study was to investigate the essential oil composition and the antioxidant evaluation of P. inuloides growing in the Algerian Sahara. The chemical composition was determined using GC-MS method, and antioxidant properties were estimated by DPPH and β-carotene assays. The major components were δ-cadinene (24.0%), α-epi-cadinol (15.0%), α-...

The present study investigates the phenolic composition, antioxidant and antimicrobial activities of an ethyl acetate extract from C. triflorus L’Her. The phytochemical study on the aerial parts of C. triflorus belonging to the Fabaceae family led to the isolation and structural elucidation of 5-Hydroxy-7-O-glucosylflavone (P1), 5-Hydroxy-7-O-galac...

The chloroform and ethyl acetate extracts obtained from the aerial parts of Anthriscus vulgaris Bernh. were analyzed by gas chromatography-mass spectrometry (GC-MS). 36 components have been identified in each extract. The major constituents were 1-monooleoylglycerol (20.72%), caffeic acid (15.20%), cinnamic acid (11.31%) and benzene acetic acid (10...

Ethnopharmacology relevance: Cymbopogon schoenanthus (C. schoenanthus) and Helianthemum lippii (H. lippii) are Saharan species found in the South West of Algeria, in the region of Bechar. Both plants are used in traditional medicine to treat gastrointestinal disorders. Objective: The aim of our study was to characterize the composition of the et...

Leishmaniasis is a tropical disease caused by protozoans of the genus Leishmania. According to WHO (World Health Organization), leishmaniasis affects around 12 million people in 98 countries from Africa, Asia, America and Europe [1]. In Algeria, leishmaniasis remains a major public health problem, where, it classified cutaneous and visceral leishma...

The essential oil of the aerial parts of Thymus ciliatus (Desf.) belonging to the Lamiaceae family, was obtained by steam distillation and analyzed by GC-FID and GC-MS. 75 components were identified corresponding to 95.57% of the total oil. The major constituents of the oil were: elemol (6.80%), carvacrol (5.86%), γ-muurolene (5.18%), β-sesquiphell...

Phenolic extracts of aerial parts of Genista ferox have been characterized and evaluated for their pharmacological properties which are still not reported. The total phenol and flavonoid contents in the extracts were estimated spectrophotometrically via the Folin-Ciocalteu and aluminium chloride methods, respectively. Leaves and pods extracts showe...

Artemisia campestris L., (Asteraceae) called “Dgouft’’ in Algeria (Belhattab et al., 2011) is a medicinal and aromatic herb rich in essential oil (Chalchat et al., 2003). It is used in folk medicine for its anti-inflammatory and antimicrobial properties (Akrout et al., 2011) and against digestive troubles and gastric ulcer (Dob et al., 2005). Accor...

Leishmaniasis remains a major world health problem, and in particular, Algeria ranks second for the incidence of cutaneous leishmaniasis. Pulicaria inuloides is a well-known Arabian Peninsula medicinal plant. In the present study, the chloroform, ethyl acetate and n-butanol extracts from the roots of Pulicaria inuloides were analyzed for antioxidan...

The pro-apoptotic property of n-BuOH extract of Limonium duriusculum (BEL) and its major isolated components [apigenin (APG1) and apigenin7-O-β-D-(6''-methylglucuronide) (APG2)] were tested. The anti-proliferative IC50 of BEL and APG1 was quantified as 7.60 µg/mL and 25.74 µM respectively, while APG2 did not affect cell proliferation in HCT116 p53...

In the present study, in vivo antioxidant properties of the n-butanol extract obtained from aerial parts of Perralderia coronopifolia were investigated in term of its hepatoprotective effect of female Wistar albino rats (n, 36; average age, 48 ± 5 days; weighing 150 ± 18 g) against PCP (pentachlorphenol)-induced toxicity. PCP (20 mg/kg b.w.) and pl...

This study aimed to evaluate the in vitro antiproliferative and inhibition of oxidative DNAdamage activities of n-butanol (n-BuOH) extract of Centaurea sphaerocephala. The in vitro antioxidant activity of the ethyl acetate (EtOAc) and the n-BuOH extracts of this plant were also assayed. To investigate the antioxidant potential, extracts were tested...

Asteriscus graveolens (Forsk) Less. is a Saharan medicinal plant of Asteraceae family. A new acyclic sesquiterpene [7,12-dihydroxy-6,7-dihydro-5,(6) E-dehydronerolidol (3)] and sesquiterpene germacranolide lactone derivatives [9β-hydroxy-11β,13-dihydroparthenolide-9-O-β-D-glucopyranoside (7) and 9α-hydroxy-11β,13-dihydroparthenolide-9-O-β-D-glucopy...

This study aimed to evaluate the in vitro antiproliferative and inhibition of oxidative DNA-damage activities of n-butanol (n-BuOH) extract of Centaurea sphaerocephala. The in vitro antioxidant activity of the ethyl acetate (EtOAc) and the n-BuOH extracts of this plant were also assayed. To investigate the antioxidant potential, extracts were teste...

This study was aimed to evaluate the antidiabetic, dermatoprotective, neuroprotective and antioxidant activities of n-butanol extracts from leaves and flowers of Chrysanthemum fontanesii. β-carotene-linoleic acid, Galvinoxyl radical (GOR), ABTS, hydroxyl radical, reducing power, CUPRAC, O-phenanthroline and metal chelating assays were used to evalu...

Plants are the main sources of natural antioxidants in the form of phenolic compounds, which help human beings to deal with oxidative stress, caused by free radical damage. For this reason, the present study was carried out to evaluate the antiproliferative, antioxidant and inhibition of oxidative DNA damage activities of n-butanol extract obtained...

Background: Herbal medicines have been used in the treatment of liver diseases for a long time. The current study was elaborated to evaluate in vitro and in vivo antioxidant and anti-inflammatory effect of Lotus corniculatus (L. corniculatus) butanolic extract. Method: The in vitro antioxidant and anti-inflammatory property of L. corniculatus wa...

L'objectif de notre travail est d'étudier l’effet protecteur de l’extrait n-butanol de la plante Genista sp (Fabaceae) contre la cardiotoxicité induite par la doxorubicine chez des rats Wistar albinos mâles en utilisant des paramètres biochimiques. Les rats ont été soumis à un traitement oral pré- et post-phylactique concomitant d'extrait n-butanol...

Natural products of plant source are still a necessary part of old-style therapeutic systems in developing nations. Pentachlorophenol (PCP) is a well-known organochlorine compound primarily used as a general herbicide, pesticide and wood preservative, as well as an insecticide and broad-spectrum biocide. In the present study, in vivo antioxidant p...

Background The antioxidant activity and the total phenolic and flavonoid contents of the derived extracts (chloroform, ethyl acetate and n-butanol) of the 70% hydroalcoholic extract of the aerial parts of Centaurea solstitialis growing in Algeria was assessed. The active extracts were selected for phytochemical investigations. Methods The antioxid...

Dimer of herniarin (1) together with herniarin (2), scopoletin (3), and (2E,4E)-6-(2-thienyl)-2,4-hexadien-isobutylamide (4) were isolated from the flowers of Matricaria pubescens. Their structures were established by spectroscopic data and by comparison with spectral data of known compounds. Compound 1 is reported for the first time in plant kingd...

Background: Hawthorn (Crataegus oxyacantha), a common edible plant, is widely used for the preparation of different foodstuff and is also used in traditional medicine to treat heart problems and gastrointestinal ailments. Recently, a few patents of Crataegus preparation for protective effects (prevention of cardiovascular and hepatic diseases) hav...

This study was performed on the derived fractions [chloroform (FT-C), ethyl acetate (FT-E) and n-butanol (FT-B)] of the 70% ethanol extract prepared from Frankenia thymifolia, endemic to North Africa. The fractions showed high total phenolic and flavonoid contents particularly FT-E. These results agreed with their antioxidant effects. Indeed, all t...

Convolvulus trabutianus Schweinf. & Muschl. is an endemic plant from northern Sahara used in folk medicine. Herein we report, the isolation, characterization and evaluation of the radical scavenging properties of twenty three compounds from different extracts of this species by on-line HPLC-ABTS•+ screening. These compounds include nine phenolic ac...

Two new amino acid-sesquiterpene lactone conjugates, named centaureolide A (1) and centaureolide B (2) along with a germacrane derivative (3), five flavonoids (4–8) and one quinic acid derivative (9) have been isolated from the aerial parts of Centaurea pungens (Asteraceae). Their structures were established by a combination of one- and two-dimensi...

The phytochemical investigation of both chloroform and ethyl acetate extracts of Centaurea microcarpa Coss. & Dur. led to the isolation of a new cyanogenic glucoside 6'-methacrylate prunasin (3) together with seven known compounds: hydroxy-11β,13-dihydro onopordaldehyde (1), β-sitosterol (2), daucosterol (4), nepetin (5), prunasin (6), astragalin (...

Aim and Objective: A simple catalytic method has been developed to achieve the reduction of nitroarenes with NaBH4 using diphenyl diselenide catalyst in order to obtain the azoxyarene, azoarene products under mild conditions. Materials and Methods The reduction of different substituted nitroarenes was realized in alkaline ethanolic solution using...

Abstract Cardiotoxicity, nephrotoxicity, and hepatotoxicity are the common complications of doxorubicin (DOX) chemotherapy and thus limit the use of DOX in clinic. In the present study, we investigated the protective efficacy of n-butanol extract of a Rosaceae medicinal plant (Crataegus oxyacantha) against DOX-induced cardiac, renal and hepatic to...

The aim of this study was to identify and quantify the phenolic compounds of the Ethyl acetate extract from Lepidium draba using HPLC-TOF/MS; and to investigate the antioxidant potencies. In this work, we have found that the Ethyl acetate soluble fraction of L. draba is a good source of antioxidant, which is the result of its phenolic acids and fla...