
Beñat Olave- PhD in Chemistry
- Professor (Associate) at University of the Basque Country
Beñat Olave
- PhD in Chemistry
- Professor (Associate) at University of the Basque Country
Hi! To find out more just visit Linkedin: https://www.linkedin.com/in/benatolave/
About
17
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Introduction
benat.olave@ehu.eus || +34697817659
Current institution
Additional affiliations
Education
April 2016 - October 2016
September 2015 - October 2016
September 2011 - July 2015
Publications
Publications (17)
This thesis focuses on the controlled synthesis of metallic nanoparticles in aqueous micellar
solutions formed by surface-active ionic liquids. These nanoparticle containing systems have
been used in hydrogenation reactions.
In the first and second part, different types of surface-active ionic liquids based on imidazolium
cations were successfu...
Smart membranes that respond to environmental stimuli are gaining attention because of their potential use in a variety of applications, from drug delivery to water treatment. Their surface characteristics and/or permeation properties, including pressure-driven hydraulic permeability and concentration-driven diffusional permeability, can be adjuste...
Neutrinoless double beta decay ($\beta\beta0\nu$) is a putative nuclear decay that can occur if, and only if, neutrinos are their own antiparticles. Due to the smallness of neutrino masses, the lifetime of $\beta\beta0\nu$ is expected to be larger than $10^{26}$ yr, and the tiny expected signals are deeply buried in backgrounds associated with the...
Thesis: Functional DNA in Non-Conventional Solvents
https://addi.ehu.es/handle/10810/55214
Chapter 8. DNA-based gating membranes and asymmetric catalysis
8.1. Introduction
8.2. Results and Discussion
8.2.1. DNA nanostructures on anodized aluminum oxide
8.2.2. A custom-made microfluidic diffusion cell
8.2.3. DNA-based asymmetric catalysis
8.3. Concl...
Thesis: Functional DNA in Non-Conventional Solvents
https://addi.ehu.es/handle/10810/55214
Chapter 7. Stimuli-responsiveness of DNA nanostructures
7.1. Introduction
7.2. Results and Discussion
7.2.1. Design of the layer-by-layer supersandwich build-up
7.2.2. Disassembly of the supersandwich by molecular target recognitionVI
7.2.3. Influence of mism...
Thesis: Functional DNA in Non-Conventional Solvents
https://addi.ehu.es/handle/10810/55214
Chapter 6. Selection of an ATP-Aptamer in a hydrated ionic liquid
6.1. Introduction
6.2. Results and Discussion
6.2.1. SELEX in non-physiological media
6.2.2. Binding studies of potential aptamer sequences
6.2.3. Secondary structure of novel ATP DNA-aptamers...
Thesis: Functional DNA in Non-Conventional Solvents
https://addi.ehu.es/handle/10810/55214
Chapter 5. Long-term use and function of DNA in deep eutectic solvents
5.1. Introduction
5.2. Results and Discussion
5.2.1. Long-term secondary structure of the ATP DNA-aptamer
5.2.2. The recognition capacity of the ATP DNA-aptamer
5.2.3. The recognition capa...
Thesis: Functional DNA in Non-Conventional Solvents
https://addi.ehu.es/handle/10810/55214
Chapter 4. Computational studies of a DNA double helix in mixed solvents
4.1. Introduction
4.2. Results and Discussion
4.2.1. Intermolecular interactions between solvent and DNA components
4.2.2. Solvation of a double helix in mixed solvents and their nanostr...
Thesis: Functional DNA in Non-Conventional Solvents
https://addi.ehu.es/handle/10810/55214
Chapter 3. Spectroscopic studies of DNA double helixes in mixed solvents
3.1. Introduction
3.2. Results and Discussion
3.2.1. The thermal stability of a short double helix in deep eutectic solvents
3.2.2. The nanoenvironment close to DNA via time-resolved flu...
Nucleic acids have the ability to generate advanced nanostructures in a controlled manner and can interact with target sequences or molecules with high affinity and selectivity. For this reason, they have applications in a variety of nanotechnology applications, from highly specific sensors to smart nanomachines and even in other applications such...
This study investigates the beliefs of primary school teachers about multilingual language teaching and learning approaches and examines the relationship between these beliefs and the current ideas on multilingualism. This paper reports key elements of the multilingual educational reality in the Basque Country, where a minority language (Basque), a...
The present research focuses on studying teachers’ beliefs about the complexity of learning the curricular languages in the Basque Country, with special focus on Basque, the minority language.The study adopts a mixed-methods research design. For the quantitative data, 1093 participants completed a specifically designed online questionnaire, which w...
Nucleic acids have been essential to all known life forms for millions of years, and in recent decades, their usefulness has been extended to non-biological contexts as well. The ability of DNA and RNA to form specific interactions between their bases or even other molecules has enabled their integration into various applications and the creation o...
The community approach is essential for a better response to social needs. An expression of such an approach can be educational support and reinforcement programmes and projects where volunteers accompany and create spaces for rest, socialisation and learning. This communication focuses on the case of Gipuzkoa and the work of volunteers of the UPV/...
Observation of the neutrinoless double β decay is the only practical way to establish that neutrinos are their own antiparticles¹. Because of the small masses of neutrinos, the lifetime of neutrinoless double β decay is expected to be at least ten orders of magnitude greater than the typical lifetimes of natural radioactive chains, which can mimic...
At the Basque Autonomous Country, the majority of students with foreign origin at the compulsory education are Hispanic Americans. In general, these students study in model D at the primary school, the same as native ones. However, at higher educational levels there is a huge movement of the former students towards the models A and B; in addition,...
Ionic liquids are attractive because they offer versatility in the design of organic salts. As ion-rich media, ionic liquids can control the systems properties by tuning the size, charge, and shape of the composing ions. Whilst the focus has mainly been on the potential applications of ionic liquids as solvents, they also provide innovative opportu...
Questions
Questions (3)
Does the hybridization percentage matter while measuring the melting temperature of short DNA at the spectrophotometer?
If it is the case, at low hybridization percentages, I suposse it is more recomendable to measure the melting temperature at the spectrofluorimeter with a fluorophore and quencher pair or intercalator.
Hello,
I am measuring the melting temperature of two hybridized oligomers (23 bp) with the steady state fluorescence in the spectrofluorimeter. In one strand I have a fluorophore (AF488) and in the other a quencher (BHQ), that is how it can be measured the Tm (when the temperature raises the signal too).
The thing is that when I put the DNA in presence of Ionic Liquids or DESs I observe always a melting transition at 30 ºC, 10 times less intense than the melting transition of the DNA which is also present around 50-60 ºC.
In different articles this melting transition also appears but they never explain why. Any idea?