Bela Böddi

Bela Böddi
Eötvös Loránd University · Department of Plant Anatomy

PhD, DSc Professor emeritus

About

105
Publications
20,387
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2,343
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Additional affiliations
January 2004 - present
January 2003 - December 2010
Semmelweis University
Description
  • Cooperation
August 2000 - present
Eötvös Loránd University
Description
  • Research and teaching

Publications

Publications (105)
Article
Full-text available
Nowadays, the use of algae is prevalent for both industrial and agricultural purposes. The determination of chlorophyll (Chl) content is a commonly used method for estimating the phytoplankton abundance in different water bodies or biomass density of algal cultures. The aim of the present work is to optimise the efficiency of the Chl extraction fro...
Article
Full-text available
The effects of distinct UV-A and UV-B radiations were studied on etiolated pea (Pisum sativum L.) epicotyls. Emission spectra of the native protochlorophyll and protochlorophyllide forms were measured when epicotyls were excited with 360 or 300 nm light. The UV-A (360 nm) excited mainly the non-enzyme-bound monomers of protochlorophyll and protochl...
Article
Full-text available
The recently synthesized isocyanonaphtalene derivatives ACAIN and CACAIN are fluorochromes excitable at wavelengths of around 366 nm and bind cysteine-rich proteins with hydrophobic motifs. We show that these compounds preferentially label tonoplasts in living Arabidopsis and tobacco (Nicotiana tabacum SR1) cells. ACAIN-labeled membranes co-localiz...
Article
Full-text available
Background: Respiratory diseases are mainly derived from acute and chronic inflammation of the alveoli and bronchi. The pathophysiological mechanisms of pulmonary inflammation mainly arise from oxidative damage that could ultimately lead to acute lung injury. Apigenin (Api) is a natural polyphenol with prominent antioxidant and anti-inflammatory p...
Article
Full-text available
A long-living (of up to several years) bipartite system was constructed between the unicellular green alga Chlamydomonas reinhardtii and the ascomycetous fungus Alternaria infectoria. The metabolic cooperation between the two organisms was tested with infecting A. infectoria hyphae into nitrogen starving yellow C. reinhardtii culture. After the inf...
Article
The maintenance but substantial transformation of plastids was found in lowermost hypocotyl segments of soil-grown bean plants (Phaseolus vulgaris cv. Magnum) during a 60-day cultivation period. Although the plants were grown under natural light–dark cycles, this hypocotyl segment was under full coverage of the soil in 5–7 cm depth, thus it was nev...
Article
Photosynthetic activity was identified in the under-soil hypocotyl part of 14-day-old soil-grown bean plants (Phaseolus vulgaris L. cv. Magnum) cultivated in pots under natural light-dark cycles. Electron microscopic, proteomic and fluorescence kinetic and imaging methods were used to study the photosynthetic apparatus and its activity. Under-soil...
Article
Full-text available
The photoreduction and photooxidation processes of different protochlorophyll(ide) forms were studied in the innermost leaves of cabbage (Brassica oleracea var. capitata L.) under monochromatic irradiations. Room-temperature fluorescence emission spectra were measured from the same leaf spots before and after illumination to follow the wavelength d...
Article
Full-text available
Nitrogen is an essential factor for normal plant and algal development. As a component of nucleic acids, proteins, and Chl molecules, it has a crucial role in the organisation of a functioning photosynthetic apparatus. Our aim was to study the effects of nitrogen starvation in cultures of the unicellular green alga, Chlamydomonas reinhardtii, maint...
Article
The effects of nitrogen (N)-deprivation were studied in etiolated pea plants (Pisum sativum cv. Zsuzsi) grown in shoot cultures. The average shoot lengths decreased and the stems significantly altered considering their pigment contents, 77 K fluorescence spectra and ultrastructural properties. The protochlorophyllide content and the relative contri...
Article
Protochlorophyllide (Pchlide), protochlorophyll (Pchl) and chlorophyll (Chl) contents, their distribution and native arrangements were studied in under-soil hypocotyl segments of 4-, 7- and 14-day-old bean (Phaseolus vulgaris L. cv. Magnum) seedlings. The plants were grown in general potting soil under natural illumination conditions in pots. For s...
Article
‘Chlorella’ and ‘Nannochloris’ were traditional genera of minute coccoid green algae with numerous species described in the past century, including isolates used as experimental test organisms. In the last few years, the introduction of DNA-based phylogenetic analyses resulted in a large number of taxonomic revisions. We investigated and reclassifi...
Article
The transformation of desiccoplasts into etioplasts and the parallel appearance of protochlorophyllide (Pchlide) forms were observed with transmission electron microscopy and 77K fluorescence spectroscopy, when air-dried detached leaves of the poikilochlorophyllous desiccation tolerant plant Xerophyta humilis were floated in water in the dark. Afte...
Article
The phototransformation pathways of protochlorophyllide forms were studied in 8-14-day-old leaves of dark-germinated wheat (Triticum aestivum L.) using white, 632.8 nm He-Ne laser and 654 nm laser diode light. The photon flux density (PFD) values (0.75-360 μmol photons m(-2) s(-1)), the illumination periods (20 ms-10 s) and the temperature of the l...
Article
To study if etiolation symptoms exist in plants grown under natural illumination conditions, under-soil epicotyl segments of light-grown pea (Pisum sativum) plants were examined and compared to those of hydroponically dark-grown plants. Light-, fluorescence- and electron microscopy, 77 K fluorescence spectroscopy, pigment extraction and pigment con...
Article
Full-text available
To study the formation of the photosynthetic apparatus in nature, the carotenoid and chlorophyllous pigment compositions of differently developed leaf primordia in closed and opening buds of common ash (Fraxinus excelsior L.) and horse chestnut (Aesculus hippocastanum L.) as well as in closed buds of tree of heaven (Ailanthus altissima P. Mill.) we...
Article
We describe Chloroparva pannonica Somogyi, Felföldi & Vörös gen. et sp. nov., a new trebouxiophycean picoplanktonic alga isolated from a turbid, shallow soda pan in Hungary. The cells are spherical to oval, less than 2 mm in diameter, with simple ultrastructure typical to small green algae. Cells divide by autosporulation, forming two daughter cell...
Article
Full-text available
A long-living artificial tripartite symbiosis involving a green alga (Chlamydomonas), a bacterium (Azotobacter) and a fungus (Alternaria) was established on carbon- and nitrogen-free medium. The basis of the interdependence is the complementation of photosynthetic CO2 assimilation and atmospheric nitrogen fixation. Green color of the colonies indic...
Article
The regeneration and stability of the NADPH:protochlorophyllide oxidoreductase (POR, EC 1.3.1.33) enzyme complexes were studied in bleached epicotyls of 9-day-old dark-germinated pea (Pisum sativum L. cv. Zsuzsi) seedlings. Middle segments were illuminated with 1300 micromol m(-2) s(-1) photon flux density (PFD) white light and subsequently incubat...
Article
Using fluorescence imaging and fluorescence spectroscopic methods, the localisation of the laticifers and the native spectral properties of the milk were studied in various organs of greater celandine (Chelidonium majus L.). Direct measurements on tissue pieces (without the extraction and the separation of the components) provided information about...
Article
Type-II, singlet oxygen-mediated photosensitized damage has already been shown to occur in epicotyls of dark-germinated pea (Pisum sativum L.) seedlings upon illumination, resulting in fast turgor loss and wilting. In this study we show evidence that the palette of reactive oxygen species (ROS) is more complex. Hydrogen peroxide, superoxide and hyd...
Article
The last steps of chlorophyll (Chl) biosynthesis were studied at different light intensities and temperatures in dark-germinated ginkgo (Ginkgo biloba L.) seedlings. Pigment contents and 77 K fluorescence emission spectra were measured and the plastid ultrastructure was analysed. All dark-grown organs contained protochlorophyllide (Pchlide) forms w...
Article
Fluorescence spectra and fluorescence lifetimes of protochlorophyll (Pchl) were measured in organic solvents having different physical and chemical properties and were analyzed taking into account the nonspecific (dependent on bulk solvent parameters), and specific (e.g. H bonds, Mg coordination) solvent-solute interactions. The energy of the fluor...
Article
Low-temperature (77K) fluorescence emission spectra of 100 dark-grown pea (Pisum sativum L.) seedlings of various ages were measured. The spectra of the 100 leaf samples were collected into a separate data group and those of epicotyls formed another one. This group was divided into three sub-groups as spectra of uppermost, middle and lowermost 3 cm...
Article
Abstract— Partial phototransformation caused the formation and successive accumulation of spectral forms of chlorophyllide in etioplast inner membrane fractions from wheat (Triticum aestivum L. cv. Walde), Low-temperature fluorescence emission and circular dichroism spectra showed the formation of two chlorophyllide forms with emission maxima at 68...
Chapter
Full-text available
When exposed to salt stress, leaves from dark-grown wheat seedlings (Triticum aestivum, cv. Giza 168) showed reduced accumulation of chlorophyll during irradiation. Plastids from salt-treated leaves had highly swollen prothylakoids (PTs) whereas the prolamellar bodies (PLBs) were regular. The long-wavelength Pchlide form emitting at 656 nm was inta...
Article
The activation energy and activation volume of the spectral blue shift subsequent to protochlorophyllide phototransformation (called Shibata shift in intact leaves) were studied in prolamellar body (PLB) and prothylakoid-(PT)-enriched membrane fractions prepared from dark-grown wheat (Triticum aestivum, L.) leaves. The measurements were done at 20,...
Article
Full-text available
Etiolation symptoms and the greening process are usually studied on dark-germinated seedlings and this raises the question--can these results be generalized for plants growing under field conditions? This work examines various aspects of the plastid differentiation under the covering of the achene wall, which often remains attached to the cotyledon...
Article
The steady-state and time-resolved fluorescence characteristics of protochlorophyll (Pchl) dissolved in neat Triton X-100 and in Triton X-100 micelles were investigated, and the fluorescence lifetimes of different Pchl spectral forms were studied. Varying the concentration of Pchl or diluting the micellar solutions either with a buffer or with a mi...
Article
Full-text available
High salinity causes ion imbalance and osmotic stress in plants. Leaf sections from 8-d-old dark-grown wheat (Triticum aestivum cv. Giza 168) were exposed to high salt stress (600 mM) and the native arrangements of plastid pigments together with the ultrastructure of the plastids were studied using low-temperature fluorescence spectroscopy and tran...
Article
SUMMARY To understand the phenomenon by which infection of seed-transmitted Barley stripe mosaic virus (BSMV) alters membrane structures and inhibits protochlorophyllide biosynthesis of dark-grown barley (Hordeum vulgare L.) plants, we analysed the presence of NADPH:protochlorophyllide oxidoreductase (POR, EC 1.3.1.33) and the galactolipid content...
Article
The effects of high concentrations of Hg (2+) (10 (-2) M and 10 (-3) M) were investigated on the ultrastructure and on the light-induced transformation of isolated prolamellar bodies (PLBs) of dark-grown wheat leaves. Our earlier work on wheat leaf homogenates ( , Plant Biology 6, 358 - 368) showed that, depending on the concentration, Hg (2+) reac...
Article
The transmission spectra of bud scales of 14 woody species and the 77 K fluorescence emission spectra of the innermost leaf primordia of closed and opened buds of 37 woody species were studied. Pigment concentrations were determined in some species. Bud scales had low transmittance between 400 and 680 nm with a local minimum around 680 nm. Transmit...
Article
An accompanying paper reports the accumulation of photoactive protochlorophyllide (Pchlide) in the innermost leaf primordia of buds of many tree species. In this paper, we describe plastid differentiation, changes in pigment concentrations and spectral properties of bud scales and leaf primordia of horse chestnut (Aesculus hippocastanum L.) from Ja...
Article
Artificial formation of flash-photoactive oligomeric protochlorophyllide complexes was found in etiolated pea (Pisum sativum L. cv. Zsuzsi) epicotyl homogenates containing glycerol (40% v/v) and sucrose (40% m/v). The 77 K fluorescence emission spectra indicated that the ratio of the 644 and 655 nm emitting forms to the 636 nm form increased during...
Article
Low-temperature fluorescence emission spectra of 6.5-day-old dark-grown epicotyls of pea (Pisum sativum) revealed the presence of protochlorophyll(ide). The upper part of the epicotyl contained 30% of the protochlorophyll(ide) content per fresh weight found in pea leaves, whereas the lower part contained 3%. Three discrete spectral forms of protoch...
Article
The in vivo absorbance spectrum of the inner seed coat of Cyclanthera explodens Naud. showed a main peak in the red region at 671 nm and a weak shoulder at about 640 nm. The pigments were extracted with acetone. separated by paper chromatography and analysed spectrophotometrically. The only detectable pigment was protochlorophyll. The in vivo fluor...
Article
Protochlorophyll was found in roots of dark-grown plants of seven species investigated. It was identified by absorbance and fluorescence spectra of acetone and ether extracts. Chlorophyll was also found in roots of one pea species. The concentration of protochlorophyll was usually highest in young root tips and decreased upwards along the roots. Th...
Article
A comparison was performed of the tetrapyrrole transformations that occur upon irradiation of epicotyl or leaves of dark-grown Pisum sativum L. (var. Zsuzsi, Hungary). High performance liquid chromatography analysis after continuous or flash-irradiation showed that the biosynthetic pathway from protochlorophyllide (Pchlide) to chlorophyll (Chl) a w...
Article
The phototransformation of protochlorophyllide forms was studied in epicotyls of dark-germinated pea (Pisum sativum L. cv. Zsuzsi) seedlings. Middle segments were illuminated with white or 632.8 nm laser flash or continuous light at room temperature and at −15°C. At low light intensities, photoreduction could be distinguished from bleaching. 77 K f...
Article
The effect of Barley stripe mosaic virus (BSMV) infection was studied oil the ultrastructure of etioplasts and the greening process of barley (Hordeum vulgare cv. Pannonia) plants infected by seed transmission. The leaves of 7-11-day old etiolated seedlings were examined with transmission electron microscopy and absorption spectroscopy. The etiopla...
Article
Full-text available
Possible mechanisms behind the light-induced wilting of dark-germinated pea (Pisum sativum L.) epicotyls were studied. Illumination with photosynthetically active radiation caused a fast turgor loss and wilting in the middle segments of the epicotyls accompanied by accumulation of water in the intercellular cavities. During this process, room tempe...
Article
Full-text available
The outer leaves of white cabbage act as optical filters and decrease the light intensity considerably inside the cabbage head. As a consequence, the inner leaf primordia develop under prolonged etiolation conditions. The detailed analysis of plastid ultrastructure and chlorophyll biosynthesis of the different leaf layers of cabbage head was done i...
Article
Photoreduction and interconversion processes of different protochlorophyllide complexes were studied in epicotyl segments of dark-germinated pea (Pisum sativum L.) seedlings illuminated with low-intensity white or 632.8 nm laser light. Analyses of 77 K fluorescence emission spectra showed the direct phototransformation of the monomer, 636 nm emitti...
Article
Most studies on chlorophyll biosynthesis and greening are carried out on angiosperm leaves. Unlike angiosperms, most gymnosperms can synthesize chlorophyll in the dark and cannot be etiolated. Ginkgo (Ginkgo biloba L.) is an exception. In this work, the greening of the stems and the leaves of ginkgo were analyzed. Pigment content changes, changes i...
Article
Plastid differentiation and the greening process are usually studied in etiolated leaves. In this work, we studied stems or stem related organs of various plant species. Electron microscopy studies combined with fluorescence spectroscopy showed the formation of protochlorophyllide forms similar to those of etiolated leaves but their ratio had a big...
Article
The contents of protochlorophyllide, protochlorophyll and chlorophyll together with the native arrangements of the pigments and the plastid ultrastructure were studied in different leaf layers of white cabbage (Brassica oleracea cv. capitata) using absorption, 77 K fluorescence spectroscopy and transmission electron microscopy. The developmental st...
Article
The molecular background of Hg2+-induced inhibition of protochlorophyllide (Pchlide) photoreduction was investigated in homogenates of dark-grown wheat leaves. Our earlier work showed that 15 min incubation with 10-2 M Hg2+ completely inhibits the activity of NADPH: Pchlide oxidoreductase. Detailed analysis of spectra recorded at 10 K indicated the...
Article
Cuttings of grapevine (Vitis vinifera L. cv. Chardonnay) were dark-forced at least three weeks. Pigment contents, 77 K fluorescence emission, excitation spectra of the leaves, petioles, stems, transmission electron micrographs of the etioplasts from leaves, the chlorenchyma tissues of the stems were analysed. The dark-grown leaves, stems contained...
Article
The Shibata shift was analyzed in flash irradiated wheat (Triticum aestivum, L., cult. MV17) leaf homogenates in the pressure range of 0.1 to 500 MPa, at temperatures of 20, 30 and 40 degrees C. The kinetics of the blue shift (called Shibata shift in case of intact leaves) was followed by repeated recording of fluorescence emission spectra after ph...
Article
Full-text available
Three alkalitolerant bacterial strains were isolated from the surface of decomposing rhizomes of reed [Phragmites australia (Cav.) Trin. et Steudel] in Lake Fertö (Hungary). Cells of the novel isolates were Gram-negative, motile rods and formed star-shaped aggregates. They were facultatively anaerobic and chemo-organotrophic. Bacteriochlorophyll a...
Article
The steps of protochlorophyllide (Pchlide) photoreduction and subsequent chlorophyllide (Chlide) transformations which occur in the seconds to minutes time-scale were studied using a diode array spectrofluorometer in dark-grown barley leaves. The intensity of the excitation light was varied between 3 and 2,500 micromol m(-2) s(-1) and a series of f...
Chapter
In dark-grown higher plants the chlorophyll biosynthetic pathway stops at protochlorophyllide because the enzyme, NADPH-pchlide oxidoreductase (POR), responsible for the transformation of pchlide into chlorophyllide (chlide) requires light for catalysis. As a consequence, the transformation of pchlide into chlorophyllide can be triggered with illum...
Article
Full-text available
Chlorophyll-a content is widely used as an indicator of the quality of freshwater bodies. Quantification of chlorophyll-a is a routine procedure in the test laboratories of water works, and in research laboratories. Although attempts have been made to standardise the measurement procedure, there are nonetheless many procedures currently in use. Thi...
Article
The photochemical activity of NADPH:protochlorophyllide oxidoreductase (POR) was studied in etiolated wheat (Triticum aestivum, L., cult. MV17) leaf homogenates. The kinetics of the transformation of protochlorophyllide into chlorophyllide was detected by fluorescence intensity changes at 690 nm (formation of chlorophyllide) and 655 nm (decay of pr...
Article
The relationship between the structural and functional changes of the photosynthetic apparatus in the flower bud of Lilium longiflorum during chloroplast-chromoplast transition was examined. Compared with green petals, there was a five-fold increase of the carotenoid content in yellow petals, whereas the chlorophyll content decreased five-fold. Abs...
Article
The effect of Hg++ was studied on the arrangement and photoactivity of NADPH:protochlorophyllide oxidoreductase (POR) in homogenates of dark-grown wheat (Triticum aestivum L.) leaves. 77 K fluorescence emission spectra of the homogenates were recorded before and after the irradiation of the homogenates and the spectra were deconvoluted into Gaussia...
Article
The effect of barley stripe mosaic hordeivirus (BSMV) was studied on the ultrastructure of etioplasts, protochlorophyllide forms and the greening process of barley (Hordeum vulgare cv. Pannónia) plants infected by seed transmission. The leaves of 7- to 11-day-old etiolated seedlings were examined by transmission electron microscopy, fluorescence an...
Article
The kinetics of protochlorophyllide phototransformation and of the subsequent blue shift were studied in homogenates prepared from etiolated wheat leaves using fluorescence spectroscopy at high pressures. The pressure dependence of processes was studied between 0.1 and 400 MPa. The activation volumes were calculated from the pressure dependence of...
Article
Full-text available
Effects of water deficit on the chlorophyllide (Chlide) transformation pathway were studied in etiolated barley (Hordeum vulgare) leaves by analyzing absorption spectra and 77-K fluorescence spectra deconvoluted in components. Chlide transformations were examined in dehydrated leaves exposed to a 35-ms saturating flash triggering protochlorophyllid...
Article
Full-text available
Protochlorophyllide (Pchlide) and protochlorophyll (Pchl) were found in epicotyls of 14-day-old dark-germinated seedlings and in 100-day-old dark-grown stems of red oak (Quercus rubra L.). Fluorescence spectroscopy measurements of epicotyls at 77 K showed that the majority of Pchlide and Pchl is present as a shorter wavelength-emitting monomer with...
Article
Full-text available
Protochlorophyllide forms, their phototransformation in the greening process and the ultrastructure of the etioplasts were studied in BSMV-infected barley plants. The aim of this work was to determine whether the chlorotic symptoms caused by the infection of this virus result from inhibition of chlorophyll biosynthesis. 77 K fluorescence emission s...
Article
Protochlorophyllide contents and the spectral properties together with photoactivities of native protochlorophyllide forms have been studied in dark-forced stems of 26 and epicotyls or hypocotyls of 9 plant species. The 77 K fluorescence emission spectra show that a form emitting at 629-631 nm is general in these organs. Besides this short-waveleng...
Article
The fluorescence properties and role in energy transfer of protochlorophyllide (Pchlide) forms were studied in dark-grown wheat leaves by conventional and laser excited high resolution methods in the 10 K–100 K temperature range. The three major spectral bands, with emission maxima at 633, 657 (of highest intensity) and 670 nm as Bands I, II, and I...
Article
Protochlorophyllide, chlorophyll a and chlorophyll b have been found with HPLC and 77 K fluorescence spectroscopy in the lowermost (30 mm) section of epicotyls and in the first stipulas of dark-grown pea seedlings. The total amount of chlorophylls is as high as 0.1 mu g/plant but there are also plants having only traces of chlorophylls. The amount...
Article
The fast (1 min) regeneration process of the photoactive Pchlide forms after a light flash was studied in etiolated wheat leaves, and this process was simulated in vitro by incubating etioplast inner membranes of wheat with excess NADPH or NADP+. The 77 K fluorescence spectra were recorded after flash illumination, dark incubation and a subsequent...
Article
The spectral properties of the protochlorophyllide forms in the epicotyls of dark-grown pea seedlings have been studied in a temperature range, from 10 to 293 K with conventional fluorescence emission and excitation spectroscopy as well as by fluorescence line narrowing (FLN) at cryogenic temperatures. The conventional fluorescence techniques at lo...
Article
Room-temperature fluorescence emission and excitation spectra of 3-day or 10-day old dark-grown bean (Phaseolus vulgaris L. cv Commodore) leaves were measured. The excitation light was defocused in such way that only a low rate of phototransformation took place and protochlorophyllide (Pchlide) forms could be detected. The spectra were resolved int...
Article
For a better understanding of the origin of different cold stress responses of certain maize lines, low-temperature fluorescence spectroscopy and electron microscopy was done on dark-grown and illuminated leaves. The plants of both lines were grown in darkness at 25°C for 7 days, then kept at 12°C for 6 hours. After cutting out non-illuminated samp...
Article
Room-temperature fluorescence emission and excitation spectra of 3-day or 10-day old dark-grown bean (Phaseolus vulgaris L. cv Commodore) leaves were measured. The excitation light was defocused in such way that only a low rate of phototransformation took place and protochlorophyllide (Pchlide) forms could be detected. The spectra were resolved int...
Article
Low-temperature fluorescence emission spectra of epicotyls of 6.5-day-old dark-grown seedlings of pea (Pisum sativum L.) showed the dominance of short-wavelength protoch lorophyllide forms with emission maxima at 629 and 636 nm, respectively. The presence of long-wavelength protochlorophyllide with emission maxima around 650 nm was just detectable....
Article
The paper describes the basic problems occurring in absorption and fluorescence spectroscopy and gives advices how to understand or correct the signal modifications appearing very often by measuring different biological samples. In the second part a short description is given about the connection between the electronic structure of chlorophyll type...
Article
Leaves and chloroplast suspensions of severely and slightly iron deficient cucumber (Cucumis sativus L.) plants were characterized by low-temperature fluorescence emission spectroscopy and Deriphat polyacrylamide gel electrophoresis. The emission spectra of the chloroplast suspensions were resolved into Gaussian components and those changes induced...
Article
Low-temperature fluorescence emission and excitation analyses revealed four discrete spectral forms of protochlorophyllide and/or protochlorophyll in the etioplast inner membranes isolated from leaves of wheat (Triticum aestivum cv. Kosack). The emission spectra were recorded with various excitation wavelengths between 400 and 500 nm. The excitatio...
Article
Low-temperature (77 K) fluorescence emission spectra of dark-grown leaves from 12 different plant species were measured, corrected and analysed at different stages of development. The second and fourth derivative of the spectra indicated the peak positions of possible spectral bands, which were used for resolution of the fluorescence spectra into g...
Chapter
In this paper we describe the early light-induced changes of the organization of the chlorophyllous pigments as etioplasts of dark-grown wheat leaves (Triticum aestivum) develop to chloroplasts. Based on results of both in vitro and in vivo experiments, we present our view on how changes in the state of protochlorophyllide (PChlide) and chlorophyll...
Chapter
In dark-grown leaves the plastid development is arrested at the etioplast level with the formation of typical prolamellar bodies (PLBs) and prothylakoids (PTs). The dominating protein of isolated PLBs is NADPH-protochlorophyllide oxidoreductase (PChlide-reductase). The PLBs also contain most of the phototransformable PChlide (Lindsten et al., 1988;...
Article
Abstract— Irradiation of an etioplast inner membrane fraction caused the transformation of two photoactive spectroscopically different protochlorophyllide forms into two chlorophyllide forms. A weak light flash, 6% of a saturating flash, preferentially caused the formation of a short wavelength chlorophyllide form absorbing at 672 nm and emitting a...
Article
Solid films were prepared from diethyl ether solutions of divinylprotochlorophyllide on glass surfaces and then treated with vapours of acetone. The transformation of the films and the appearance of different spectroscopic forms of divinylprotochlorophyllide were observed by absorption spectroscopy. Computer analysis allowed resolution of the absor...
Chapter
Pioneering investigations of the chlorophyll biosynthesis have shown that the knowledge of the primary chemical structure of chlorophylls and their precursors is not nearly enough for understanding of the process of this biosynthesis. This is especially true for the final steps of this process; a large body of data has been accumulated about the co...