Begoña García

Begoña García
Universidad Pública de Navarra | UPNA · Instituto de Agrobiotecnología

About

35
Publications
2,976
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1,412
Citations
Citations since 2017
17 Research Items
600 Citations
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2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120

Publications

Publications (35)
Article
Full-text available
Abstract Salmonellosis is the second most common food-borne zoonosis in the European Union, with pigs being a major reservoir of this pathogen. Salmonella control in pig production requires multiple measures amongst which vaccination may be used to reduce subclinical carriage and shedding of prevalent serovars, such as Salmonella enterica serovar T...
Article
Full-text available
Bacteria use two-component systems (TCSs) to sense and respond to environmental changes. The core genome of the major human pathogen Staphylococcus aureus encodes 16 TCSs, one of which (WalRK) is essential. Here we show that S. aureus can be deprived of its complete sensorial TCS network and still survive under growth arrest conditions similarly to...
Article
Full-text available
RNA-binding proteins (RBPs) are essential to fine-tune gene expression. RBPs containing the cold-shock domain are RNA chaperones that have been extensively studied. However, the RNA targets and specific functions for many of them remain elusive. Here, combining comparative proteomics and RBP-immunoprecipitation-microarray profiling, we have determi...
Article
Full-text available
Many bacteria build biofilm matrices using a conserved exopolysaccharide named PGA or PNAG (poly-β-1,6-N-acetyl-D-glucosamine). Interestingly, while E. coli and other members of the family Enterobacteriaceae encode the pgaABCD operon responsible for PGA synthesis, Salmonella lacks it. The evolutionary force driving this difference remains to be det...
Data
Strains and plasmids used in this study. (PDF)
Data
Analysis of PGA synthesis dependence on c-di-GMP. Quantification of PGA exopolysaccharide production by dot blot. Serial dilutions (1/10) of the samples were spotted onto nitrocellulose membranes and PGA production was detected with specific anti PIA/PNAG antibodies. UD; undiluted sample. (A) Dot blot analysis of the PGA accumulated by S. Enteritid...
Data
Oligonucleotides used in this study. (PDF)
Data
Strains WT PphoP::adrA and ΔbcsA PphoP::adrA PcL::pga produce cellulose and PGA, respectively, in a phoP dependent fashion. A cellulose based biofilm is produced by WT PphoP::adrA after overnight growth in low-Mg2+ liquid medium (top panel). An aggregate of ΔbcsA PphoP::adrA PcL::pga bacteria can be observed at the bottom of the tube as Mg2+ concen...
Data
Fitness of ΔbcsA versus the wild type or ΔbcsA PcL::pga strains. The competitive fitness of ΔbcsA in co-culture with either the wild type or ΔbcsA PcL::pga was determined by combining the two strains in LB broth, incubating at 37°C and enumerating each strain over time. (EPS)
Data
Gene neighborhood analysis for pgaA, as provided in the STRING database [36]. Results for Enterobacteriaceae genomes are fully displayed, while those from other groups are presented in a collapsed mode. (EPS)
Data
Hydrophobicity analysis of macrocolony biofilms formed by WT PcL::adrA and ΔbcsA PcL::adrA pJET::pga. (A) The cellulose overproducing strain, WT PcL::adrA, the PGA overproducing strain, ΔbcsA PcL::adrA pJET::pga and the control strain, ΔbcsA PcL::adrA, were incubated on LB agar or LB agar Cb media for 48h at 28°C. A bent tip was used to visualize t...
Data
Synthesis of PGA by a Salmonella strain that constitutively expresses the pgaABCD operon from the chromosome. Comparison of the PGA accumulated by WT PcL::pga and WT pJET::pga, expressing the pgaABCD operon from the chromosome or from a plasmid, respectively, after 24 hours of growth at 37°C in LB or LB Cb media. Serial dilutions (1/10) of the samp...
Data
PGA is overproduced by E. coli MG1655 when the pgaABCD operon is expressed from a constitutive promoter. Dot blot analysis of the PGA accumulated by E. coli MG1655, a pgaC mutant and a derivative of E. coli MG1655 in which the pgaABCD operon was placed under the control of the constitutive promoter PcL, after 24 hours of growth at 37°C in LB media....
Data
Phylogenetic trees of the analyzed proteins. First column indicates the protein name, and the second column is the phylogenetic tree in standard newick format. Labels indicate species names, as retrieved from NCBI, followed by an underscore separator and the gene identifier. (PDF)
Article
Full-text available
Salmonellosis is one of the most important bacterial zoonotic diseases transmitted through the consumption of contaminated food, with chicken and pig related products being key reservoirs of infection. Although numerous studies on animal vaccination have been performed in order to reduce Salmonella prevalence, there is still a need for an ideal vac...
Data
Representative IFN-γ and TNF-α/CD4 staining for ΔXIII stimulated splenocytes. 28 days after immunization, splenocytes from control and ΔXIII immunized mice were plated and stimulated for 6 hours with medium, 106 or 107 cfu of heat-killed ΔXIII bacteria. Cells were surface stained with anti-CD4-FITC antibody and intracellularly stained with anti IFN...
Data
Strains used in this study. (DOCX)
Article
Full-text available
The Staphylococcus aureus biofilm mode of growth is associated with several chronic infections that are very difficult to treat due to the recalcitrant nature of biofilms to clearance by antimicrobials. Accordingly, there is an increasing interest in preventing the formation of S. aureus biofilms and developing efficient anti-biofilm vaccines. Give...
Article
Biofilm development is recognized as a major virulence factor underlying most chronic bacterial infections. When a biofilm community is established, planktonic cells growing in the surroundings of a tissue switch to a sessile lifestyle and start producing a biofilm matrix. The initial steps of in vivo biofilm development are poorly characterized an...
Article
Full-text available
Cyclic di-GMP (c-di-GMP) is a secondary messenger that controls a variety of cellular processes including the switch between a biofilm and a planktonic bacterial lifestyle. This nucleotide binds to cellular effectors in order to exert its regulatory functions. In Salmonella, two proteins, BcsA and YcgR, both of them containing a c-di-GMP binding Pi...
Article
Full-text available
The Rcs phosphorelay pathway is a complex signaling pathway involved in the regulation of many cell surface structures in enteric bacteria. In response to environmental stimuli, the sensor histidine kinase (RcsC) autophosphorylates and then transfers the phosphate through intermediary steps to the response regulator (RcsB), which, once phosphorylat...
Article
Full-text available
The Extradomain A from fibronectin (EDA) has an immunomodulatory role as fusion protein with viral and tumor antigens, but its effect when administered with bacteria has not been assessed. Here, we investigated the adjuvant effect of EDA in mice immunizations against Salmonella enterica subspecies enterica serovar Enteritidis (Salmonella Enteritidi...
Data
Full-text available
Susceptibility of Salmonella Enteritidis parental and mutant strains to cationic peptides and non-immune human serum. (A) Minimal Bactericidal Concentration (MBC) to Polymyxin B; and (B) Susceptibility to conventional human serum, with respect to heat-inactivated serum. Results are expressed as the mean and SD (n = 6) of Polymyxin B concentration (...
Data
Full-text available
Flow cytometry of bacterins B-SEΔgal, B-SEΔwaaL and B-SEwt labeled with carboxyfluorescein succinimidyl ester (CFSE). Unlabeled SE-wt bacterins (B-SEwt) were used as negative control.
Data
Dose–response assay with Salmonella Enteritidis 3934 (SE-wt) strain in mice.
Article
Fresh fruit and vegetables are important components of a healthy and balanced diet. However, they are increasingly being recognized as important vehicles for transmission of human pathogens that were traditionally classified as zoonotic. There is a significant gap in our knowledge and understanding of the mechanisms by which human pathogens coloniz...
Article
Full-text available
Staphylococcus aureus can establish chronic infections on implanted medical devices due to its capacity to form biofilms. Analysis of the factors that assemble cells into a biofilm has revealed the occurrence of strains that produce either a polysaccharide intercellular adhesin/poly-N-acetylglucosamine (PIA/PNAG) exopolysaccharide- or a protein-dep...
Article
Full-text available
Bacteria have developed an exclusive signal transduction system involving multiple diguanylate cyclase and phosphodiesterase domain-containing proteins (GGDEF and EAL/HD-GYP, respectively) that modulate the levels of the same diffusible molecule, 3'-5'-cyclic diguanylic acid (c-di-GMP), to transmit signals and obtain specific cellular responses. Cu...
Article
We evaluated the efficacy of surface components enriched hot saline extracts (HE) from parental and two isogenic rough mutant strains of Salmonella Enteritidis as subcellular vaccine candidates. By a randomized mutagenesis approach from a clinical isolate of S. Enteritidis there were selected two rough mutants defective in LPS synthesis (R1 and R2...
Article
Salmonella enterica serovar Typhimurium is capable of producing cellulose as the main exopolysaccharide compound of the biofilm matrix. It has been shown for Gluconacetobacter xylinum that cellulose biosynthesis is allosterically regulated by bis-(3',5') cyclic diguanylic acid, whose synthesis/degradation depends on diguanylate cyclase/phosphodiest...
Article
We report here a new screening method based on the fluorescence of colonies on calcofluor agar plates to identify transposon insertion mutants of Salmonella enteritidis that are defective in biofilm development. The results not only confirmed the requirement of genes already described for the modulation of multicellular behaviour in Salmonella typh...

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