Beatriz L Gómez

Beatriz L Gómez
Universidad del Rosario | UR · Escuela de Medicina y Ciencias de la Salud

PhD

About

90
Publications
13,416
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2,666
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Additional affiliations
September 2011 - August 2017
Universidad del Rosario
Position
  • Professor

Publications

Publications (90)
Article
Full-text available
Background: The Americas are home to biologically and clinically diverse endemic fungi, including Blastomyces, Coccidioides, Emergomyces, Histoplasma, Paracoccidioides and Sporothrix. In endemic areas with high risk of infection, these fungal pathogens represent an important public health problem. Objectives: This report aims to summarise the ma...
Article
Full-text available
A sandwich enzyme immunoassay (EIA) for the detection of Histoplasma antigens (Ag) in urine, developed by Optimum Imaging Diagnostics (OIDx) was evaluated. A verification using a standardized reference panel of urine samples found sensitivity of 92%, specificity of 32% and accuracy of 51%. In this study, the OIDx Histoplasma urinary Ag EIA displaye...
Article
Full-text available
Histoplasmosis is one of the most frequent causes of fungal respiratory infection in endemic regions, has a broad spectrum of clinical manifestations and can present in several forms. The extent of disease is determined by the number of conidia inhaled, the immune response of the host and the integrity of the respiratory tract. From an initial and...
Article
Full-text available
Histoplasmosis is a major cause of mortality in people living with HIV (PLHIV). Rapid methods to diagnose Histoplasma capsulatum disease could dramatically decrease the time to initiate treatment, resulting in reduced mortality. The aim of this study was to validate a MiraVista® Diagnostics (MVD) Histoplasma urine antigen lateral flow assay (MVD LF...
Preprint
Full-text available
A sandwich enzyme immunoassay (EIA) for the detection of Histoplasma antigens (Ag) in urine, developed by Optimum Imaging Diagnostics (OIDx) was evaluated. A verification using a standardized reference panel of urine samples found sensitivity of 92%, specificity of 32% and accuracy of 51%. In this study, the OIDx Histoplasma urinary Ag EIA displaye...
Article
Full-text available
Candida auris, first described in 2009, is an opportunistic pathogenic yeast that causes nosocomial outbreaks around the world, with high mortality rates associated with therapeutic failure. In this study, we evaluated the pathogenicity of 107 isolates from two cities in Colombia, associated with fungemia or colonization processes; to achieve this,...
Article
Full-text available
Histoplasmosis is a frequent fungal opportunistic infection in people living with HIV (PLHIV), associated every year to a total of 5% to 15% of AIDS-related deaths among this population. In 2020, the first global guidelines for diagnosing and managing disseminated histoplasmosis among PLHIV was published. This document recommends (1) detection of c...
Article
Full-text available
Purpose of Review The aim of this report is to summarize the conclusions of the II Regional Meeting on Histoplasmosis in the Americas held in Manaus, Brazil, on March 22–24, 2019. Recent Findings Persons living with advanced HIV are at high risk for developing histoplasmosis. Clinical signs and symptoms of this disease are often non-specific, maki...
Article
Full-text available
Background: Progressive disseminated histoplasmosis (PDH) is an important cause of mortality in persons living with HIV (PLHIV), especially in countries where patients have limited access to antiretroviral therapies and diagnostic testing. Objective: A Lateral Flow Assay (LFA) to detect Histoplasma capsulatum antigen in serum developed by MiraVi...
Article
Full-text available
The diagnosis of fungal Neglected Tropical Diseases (NTD) is primarily based on initial visual recognition of a suspected case followed by confirmatory laboratory testing, which is often limited to specialized facilities. Although molecular and serodiagnostic tools have advanced, a substantial gap remains between the desirable and the practical in...
Article
Full-text available
Disseminated histoplasmosis is a major opportunistic infection of HIV-infected patients, killing thousands in Latin America each year. Yet, it remains a neglected disease that is often confused with tuberculosis, for lack of simple, affordable, and rapid diagnostic tools. There is great heterogeneity in the level of histoplasmosis awareness. The pu...
Article
Full-text available
Background: The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia. Methods: Sixty-seven Candida isolates were tested by; API® 20C-AUX, V...
Article
Full-text available
Histoplasmosis is an important cause of mortality in patients with AIDS, especially in countries with limited access to antiretroviral therapies and diagnostic tests. However, many disseminated infections in Latin America go undiagnosed. A simple, rapid method to detect Histoplasma capsulatum infection in endemic regions would dramatically decrease...
Article
Full-text available
A total of 23/45 (51%) patients with AIDS and histoplasmosis from Medellín, Colombia had other infections. Tuberculosis was the most common (n = 16/23, 70%). Pneumocystosis and cryptococcosis were found in three patients each (13%), bacterial infection and cytomegalovirus occurred each in two patients (9%) while toxoplasmosis, herpes virus and esop...
Chapter
Endemic dimorphic infections are acquired by inhalation of fungal spores which undergo a thermal transition to a yeast-like phase in the host. The causative organisms are geographically restricted and are isolated from the environment; likewise, the infections are associated with people living in, or visiting, these endemic regions. The clinical pr...
Article
Full-text available
Histoplasmosis is considered one of the most important endemic and systemic mycoses worldwide. Until now few molecular techniques have been developed for its diagnosis. The aim of this study was to develop and evaluate three real time PCR (qPCR) protocols for different protein-coding genes (100-kDa, H and M antigens) using an animal model. Fresh an...
Data
Sequences from different H. capsulatum clades used for designing primers and probes used for 100-kDa protocol. The final choices of primer and probe sequences are shown highlighted in yellow and turquoise, respectively. (PDF)
Data
Sequences from different H. capsulatum clades used for designing primers and probes used for M antigen protocol. The final choices of primer and probe sequences are shown highlighted in yellow and turquoise, respectively. (PDF)
Data
Sequences from different H. capsulatum clades used for designing primers and probes used for H antigen protocol. The final choices of primer and probe sequences are shown highlighted in yellow and turquoise, respectively. (PDF)
Article
Full-text available
A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS®)...
Article
Full-text available
Introduction: Invasive candidiasis has high impact on morbidity and mortality in hospitalized patients. Accurate and timely methods for identification of Candida species and determination of equinocandins susceptibility become a priority for laboratories of clinical microbiology. Methods: A study was performed to compare MALDI-TOF MS identificat...
Chapter
Full-text available
Paracoccidioidomycosis, the most prevalent deep mycosis of Latin America, is caused by Paracoccidioides spp., pleomorphic fungi which are present as multibudding yeast cells in infected tissues. Due to their importance to the region, these fungi and their related disease have been the subjects of intense research for over 50 years. The present chap...
Article
Full-text available
Histoplasmosis is one of the most common and deadly opportunistic infections among persons living with human immunodeficiency virus (HIV)/acquired immune deficiency syndrome in Latin America, but due to limited diagnostic capacity in this region, few data on the burden and clinical characteristics of this disease exist. Between 2005 and 2009, we en...
Article
Full-text available
Histoplasmosis is common among persons living with human immunodeficiency virus/acquired immune deficiency syndrome (PLWHA) in Latin America, but its diagnosis is difficult and often nonspecific. We conducted prospective screening for histoplasmosis among PLWHA with signs or symptoms suggesting progressive disseminated histoplasmosis (PDH) and hosp...
Article
Full-text available
Background Histoplasma capsulatum comprises a worldwide complex of saprobiotic fungi mainly found in nitrogen/phosphate (often bird guano) enriched soils. The microconidia of Histoplasma species may be inhaled by mammalian hosts, and is followed by a rapid conversion to yeast that can persist in host tissues causing histoplasmosis, a deep pulmonary...
Data
Median-joining networks showing haplotype distribution of Histoplasma capsulatum sensu. Diagrams were inferred through haplotypes from 4 different loci as follows: arf, ole1, tub1 and H-anti. Circles are proportional to haplotype frequency and numbers of haplotype (h) and Haplotype Diversity index are shown. Black dots represent a single mutation a...
Data
Population frequency and distribution of Histoplasma capsulatum sensu stricto overlaid with the epidemiological map of histoplasmosis in United States. Epidemiological map was obtained at the Centers for Disease Control and Prevention (CDC) web site: www.cdc.gov/fungal/diseases/histoplasmosis/causes.html. (PDF)
Data
Neutrality tests within Histoplasma populations. (XLSX)
Data
Mixture results of BAPS population clusterization of Histoplasma. (XLS)
Data
List of Histoplasma strains used in the present work. (XLSX)
Data
Pairedwise ΦST estimates for the main Histoplasma populations. (XLS)
Article
Full-text available
We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates...
Article
Full-text available
We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates...
Article
Full-text available
Background Candida species are the most frequently found fungal pathogens causing nosocomial disease in a hospital setting. Such species must be correctly identified to ensure that appropriate control measures are taken and that suitable treatment is given for each species. Candida albicans is causing most fungal disease burden worldwide; the chall...
Article
Full-text available
Histoplasmosis causes a significant mortality, especially persons living with human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) from developing countries where access to both appropriate diagnostic methods and antiretroviral therapy are limited. A total of 81 physicians assigned to 17 Colombian departments (states) received...
Chapter
Paracoccidioidomycosis (PCM) is an endemic disease geographically restricted to Latin America, from Mexico to Argentina; Brazil is the most endemic of all countries. Chile and the Caribbean islands are not affected. No outbreaks have been reported. Currently, phylogenetic studies divide the Paracoccidioides genus into two species, namely, Paracocci...
Article
Full-text available
We validated an antigen capture enzyme-linked immunosorbent assay (ELISA) in Colombian persons with AIDS and proven histoplasmosis and evaluated the correlation between antigenuria and clinical improvement during follow-up. The sensitivity of the Histoplasma capsulatum ELISA was 86%, and the overall specificity was 94%. The antigen test successfull...
Article
Full-text available
Diagnosis of histoplasmosis remains challenging in resource-limited regions where HIV/AIDS is epidemic and histoplasmosis is endemic. Early and rapid detection of histoplasmosis is essential to preventing morbidity and mortality, yet few diagnostic options are available in low-resource areas of the world. The aim of this review is to provide an ove...
Article
Full-text available
HIV/AIDS is not a neglected disease. Histoplasmosis is not considered a neglected disease in North America. However, in South America, it should be. It often affects neglected populations and represents a lethal blind spot of the HIV/AIDS data collection systems. Counts of new AIDS cases and AIDS-related deaths are useful to follow the epidemic; ho...
Article
The diagnosis of endemic and invasive fungal disease remains challenging. Molecular techniques for identification of fungi now play a significant and growing role in clinical mycology and offer distinct advantages as they are faster, more sensitive and more specific. The aim of this mini-review is to provide an overview of the state of the art of m...
Article
P. brasiliensis PbP27 gene encodes a protein localized in both the fungal cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with the gene's expression varying among the fungus phylogenetic species. The biological function of the native p27 has yet to be determined either during growth of the yeast or host-i...
Article
Full-text available
Currently, clinical suspicion of histoplasmosis in AIDS patients is based on the evaluation of signs and symptoms presented by the patient, and on the use of nonspecific laboratory tests. The low specificity of the signs and symptoms seriously hinders their diagnosis. The choice of the initial antimycotic therapy is based on the clinical evaluation...
Article
Full-text available
Paracoccidioidomycosis (PCM) is a systemic, endemic fungal disorder restricted to Latin America (Mexico to Argentina); Brazil accounts for the largest number of cases. Imported cases diagnosed in North America, Europe and Asia represent patients who had previously lived in recognized endemic areas. Paracoccidioides brasiliensis, the etiologic agent...
Article
Histoplasmosis is a frequent health problem in Latin American countries, but the fact that it is not a reportable disease prevents reliable estimates of its real incidence and impact on public health. Epidemiologic and clinical data remain patchy in general, and in some countries the information available is very limited. Histoplasmosis has a signi...
Article
p27 is an antigenic protein produced by Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis (PCM). Despite its unknown function, it has been suggested as a putative virulence factor, proposed as a suitable target for the design of diagnostic tools and vaccines, and considered as an enhancer in antifungal treatment of PCM. W...
Article
Several cell wall constituents, including melanins or melanin-like compounds, have been implicated in the pathogenesis of a wide variety of microbial diseases caused by diverse species of pathogenic bacteria, fungi, and helminthes. Among these microorganisms, the dimorphic fungal pathogen Paracoccidioides brasiliensis produces melanin in its conidi...
Article
This chapter covers the dimorphic members of the families Onygenaceae and Ajellomycetaceae, which include Blastomyces dermatitidis, Histoplasma capsulatum, Paracoccidioides brasiliensis, and Coccidioides immitis and C. posadasii as well as Emmonsia species. B. dermatitidis is thermally dimorphic, converting from the mold phase to the yeast phase un...
Article
Full-text available
Invasive candidiasis represents 75% of fungal infections in hospitalized patients, with reported mortalities up to 78%. The frequency of these infections varies according to the hospital services and the risk factors of the patients. In parallel, changes in the epidemiology of the Candida species have been observed, in particular variations in thei...
Article
Full-text available
Invasive candidiasis represents 75% of fungal infections in hospitalized patients, with reported mortalities up to 78%. The frequency of these infections varies according to the hospital services and the risk factors of the patients. In parallel, changes in the epidemiology of the Candida species have been observed, in particular variations in thei...
Article
Full-text available
The fungal pathogen Candida albicans produces dark-pigmented melanin after 3 to 4 days of incubation in medium containing l-3,4-dihydroxyphenylalanine (l-DOPA) as a substrate. Expression profiling of C. albicans revealed very few genes significantly up- or downregulated by growth in l-DOPA. We were unable to determine a possible role for melanin in...
Article
Full-text available
Monoclonal antibodies to Histoplasma capsulatum can modify pathogenesis. We now show that monoclonal antibody H1C to a 70-kDa antigen increases intracellular fungal growth and reduces macrophage nitric oxide release but has no effect on fungal burden or survival in murine infection. This further demonstrates the complexities of host-pathogen intera...
Article
Full-text available
DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissues is difficult and requires special protocols in order to extract small amounts of DNA suitable for amplification. Most described methods report an amplification success rate between 60 and 80%; therefore, there is a need to improve molecular detection and identification of fungi in...
Article
Full-text available
The conventional means of diagnosis of histoplasmosis presents difficulties because of the delay to the time that the diagnosis is made, indicating the need for the implementation of molecular assays. We evaluated 146 clinical samples from 135 patients suspected of having histoplasmosis using a previously reported nested PCR assay for the Histoplas...
Article
Full-text available
Histoplasma capsulatum infection causes significant morbidity and mortality in human immunodeficiency virus-infected individuals, particularly those in countries with limited access to rapid diagnostics or antiretroviral therapies. The fungus easily disseminates in persons with AIDS, resulting in progressive disseminated histoplasmosis (PDH), which...
Article
We aimed at determining involvement of extracellular matrix proteins (ECMp) and an ECM-binding adhesin (32-kDa protein) from Paracoccidioides brasiliensis, in the course of experimental paracoccidioidomycosis. BALB/c mice were infected with P. brasiliensis conidia previously incubated with soluble laminin, fibronectin and fibrinogen or a mAb agains...
Article
The limitations of classical diagnostic methods for invasive fungal infections (IFIs) have led to the development of molecular techniques to aid in the detection of IFIs. Despite good published performance, interlaboratory reproduction of these assays is variable, and no consensus has been reached for an optimal method. This publication describes t...
Article
The adhesion of microorganism to host cells or extracellular matrix (ECM) proteins is the first step in the establishment of an infectious process. Interaction between Paracoccidioides brasiliensis yeast cells and ECM proteins has been previously noted. In vivo, in the chronic phase of experimental paracoccidioidomycosis (PCM), laminin and fibronec...
Article
Full-text available
Melanins are implicated in the pathogenesis of several important human diseases. This study confirmed the presence of melanin particles in Candida albicans in vitro and during infection. Dark particles were isolated from the digestion of C. albicans cultures and from infected tissue, as established by electron microscopy and immunofluorescence tech...
Article
Full-text available
Microorganisms adhere to extracellular matrix proteins by means of their own surface molecules. Paracoccidioides brasiliensis conidia have been shown to be capable of interacting with extracellular matrix proteins. We aimed at determining the presence of fungal proteins that could interact with extracellular matrix protein and, if found, attempt th...
Article
Full-text available
Scytalidium dimidiatum is a pigmented dematiaceous coelomycete that typically causes chronic superficial skin diseases and onychomycosis, as well as deeper infections, such as subcutaneous abscesses, mycetoma, and even fungemia in immunocompromised patients. A second species, Scytalidium hyalinum, has hyaline hyphae and arthroconidia and is conside...
Article
Full-text available
La investigación sobre Paracoccidioides brasiliensis ha estado centrada en la levadura, posiblemente por la carencia de elementos distintivos en el micelio. En 1942 se anotó por primera vez la presencia de esporos en las hifas del hongo. Posteriormente, investigadores brasileros, venezolanos y argentinos demostraron la producción de ¿aleurias¿ en c...
Chapter
Fungi are ubiquitous in nature where they reside as free living organisms. Virtually all of the fungi that have been implicated in infections reside as saprobes in nature, except for the normal symbionts of humans, Malasezzia furfur and opportunistic species of Candida. While we are constantly exposed to fungi, healthy immunological competent indiv...