Beata Grzywacz

• Dr.
• Institute of Systematics and Evolution of Animals, Polish Academy of Sciences

Steppes form large zonal habitats in Asia but only consist of localised outposts in Europe. An ideal subject for the study of differences within species between the main steppe zone and the localized more western outposts is the Orthopteran Stenobothrus eurasius, widespread across the Siberian and Central Asian steppes but present only in isolated...

COVID-19 expanded rapidly throughout the world, with enormous health, social, and economic consequences. Mental health is the most affected by extreme negative emotions and stress, but it has been an underestimated part of human life during the pandemic. We hypothesized that people may have responded to the pandemic spontaneously with increased int...

The Balkan Peninsula is treated as a hotspot of biodiversity with over 40% of European bush-crickets occurring there. Poecilimon Fischer, 1853 is one of the largest Palaearctic orthopteran genera containing several species groups. One of them is the Poecilimon ornatus group (Schmidt, 1850) with 13 species and 5 subspecies. Among the group, the Poec...

The genus Poecilimon contains 145 species, widely distributed in the Palaearctic, among which the Poecilimon ornatus group has the greatest diversity in the Balkans. Despite several revisions of the genus, the systematics of the species group, and in particular, of the taxa associated with the species Poecilimon affinis, is still unsolved. Due to m...

East Africa is a hotspot of biodiversity of many orthopteran taxa, including bushcrickets. Gonatoxia Karsch, 1889 species are fully alate Phaneropterinae, which are perfectly adapted to the foliage of forests. We examined five species using combined cytogenetic and molecular data to determine the inter- and intraspecific genetic diversity. The vari...

Ophiocordyceps fungi are commonly known as virulent, specialized entomopathogens; however, recent studies indicate that fungi belonging to the Ophiocordycypitaceae family may also reside in symbiotic interaction with their host insect. In this paper, we demonstrate that Ophiocordyceps fungi may be obligatory symbionts of sap-sucking hemipterans. We...

East African mountains constitute a network of isolated habitat islands among dry savannah and are thus ideal for studying species diversification processes. This study elucidated the phylogenetic and phylogeographic relationships of all bushcricket species comprising the genus Aerotegmina. Our analysis indicated that large-scale climatic and topog...

Why do some species occur in small, restricted areas, while others are distributed globally? Environmental heterogeneity increases with area and so does the number of species. Hence, diverse biotic and abiotic conditions across large ranges may lead to specific adaptations that are often linked to a species' genome size and chromosome number. There...

Gonatoxia helleri Hemp, 2016 is one of the most widespread bush-crickets of the genus Gonatoxia Karsch, 1889 in East Africa. This species with seven large chromosomes (2n♂ = 7) differs from other representatives of the genus Gonatoxia drastically by its reduced chromosome number, the asymmetrical karyotype including karyomorphs rarely found in tett...

Severe acute respiratory syndrome coronavirus 2, the virus that causes coronavirus disease 2019 (COVID-19), has expanded rapidly throughout the world. Thus, it is important to understand how global factors linked with the functioning of the Anthropocene are responsible for the COVID-19 outbreak. We tested hypotheses that the number of COVID-19 case...

Two endemic genera of large tettigoniids are found in the Canary Islands and Madeira, which are morphologically similar to the genus Tettigonia. In this study, the karyotypes of representatives of the Glyphonotini genera Calliphona and Psalmatophanes are described for the first time. Comparison of their chromosomes with that of members of Tettigoni...

Abstract. The genus Mecistorhabdia Kiriakoff, 1953 is reviewed and one new species M. sulaki sp. n. from the Central African Republic and Cameroon (Afrotropical region) described. The holotype female of the type species M. haematoessa (Holland, 1893) is matched with a conspecifi c male using a molecular technique. M. burgessi Kiriakoff, 1957 is rem...

The cytogenetic characteristics of the grasshopper Podisma sapporensis (two races 2n = 23♂ X0/XX and 2n = 22♂ neo-XY/neo-XX) were analysed through fluorescence in situ hybridization with rDNA and telomeric DNA probes, C-banding, fluorochrome and silver staining. For the first time, samples from the neighbourhood of a hybrid population (i.e., Mikuni...

Mealybugs (Hemiptera, Coccomorpha: Pseudococcidae) are plant sap-sucking insects which require close association with nutritional microorganisms for their proper development and reproduction. Here, we present the results of histological, ultrastructural, and molecular analyses of symbiotic systems of six mealybugs belonging to the Phenacoccinae sub...

Five species belonging to the genus Gampsocleis Fieber, 1852 were analyzed using fluorescence in situ hybridization (FISH) with 18S rDNA and telomeric probes, as well as C-banding, DAPI/CMA 3 staining and silver impregnation. The studied species showed two distinct karyotypes, with 2n = 31 (male) and 2n = 23 (male) chromosomes. The drastic reductio...

The data in the Table 1 of the original version of this article were inadvertently shifted during the editing process. Therefore the entries as published in this table did not correspond to their correct data.

The possession of wings and ability to fly are a unifying character of higher insects, but secondary loss of wings is widespread. Within the bushcrickets, the subfamily Phaneropterinae (Orthoptera: Tettigonioidea) comprises more than 2000 predominantly long-winged species in the tropics. However, the roughly 300 European representatives are mainly...

In the present paper, karyotypes of 7 Japanese Podismini species, Anapodisma beybienkoi, Fruhstorferiola okinawaensis, Parapodisma caelestis, P. mikado, P. setouchiensis, P. tenryuensis, and Sinopodisma punctata (2n♂ = 21, all acrocentric), are described and compared on the basis of conventional (C-banding, DAPI/CMA3-staining, Ag-NOR) and molecular...

Two new genera of East African Acrometopini are erected: the monotypic Peronurella n. gen. with P. centralis n. sp. from Miombo woodlands in central Tanzania, and Tenerasphaga n. gen. for a morphological uniform group of species formerly included in Horatosphaga, T. chyuluensis n. sp., T. mbulu n. sp. T. nanyuki, T. nuda n. comb, T. meruensis n. co...

This is the first study to address the molecular phylogeny of East African Meconematinae. We used DNA sequence data from the three genes cytochrome oxidase subunit I (COI), 16S rRNA and histone H3 to reconstruct the relationships within the genus Amytta using Bayesian Inference and maximum likelihood. We included 45 individuals representing 19 Meco...

Members of the tribe Podismini (Orthoptera: Acrididae: Melanoplinae) are distributed mainly in Eurasia and the western and eastern regions of North America. The primary aim of this study is to explore the phylogenetic relationship of Japanese Podismini grasshoppers by comparing partial sequences of cytochrome c oxidase subunit I (COI) mitochondrial...

The present study focused on the evolution of the karyotype in four genera of the tribe Pholidopterini: Eupholidoptera Mařan, 1953, Parapholidoptera Mařan, 1953, Pholidoptera Wesmaël, 1838, Uvarovistia Mařan, 1953. Chromosomes were analyzed using fluorescence in situ hybridization (FISH) with 18S rDNA and (TTAGG) n telomeric probes, and classical...

Cytogenetical studies were performed in nine taxa (eight species and one additional subspecies) of Parnassiana and two additional closely related taxa belonging to tribe Plactyleidini. Classical (C-banding, DAPI/CMA3-staining, Ag-NOR) and molecular (fluorescence in situ hybridization with 18S rDNA probe) methods were used for cytological discrimina...

The genus Tettigonia includes 26 species distributed in the Palaearctic region. Though the Green Bush-crickets are widespread in Europe and common in a variety of habitats throughout the Palaearctic ecozone, the genus is still in need of scientific attention due to the presence of a multitude of poorly explored taxa. In the present study, we sought...

The genus Tettigonia includes 26 species distributed in the Palaearctic region. Though the Green Bush-crickets are widespread in Europe and common in a variety of habitats throughout the Palaearctic ecozone, the genus is still in need of scientific attention due to the presence of a multitude of poorly explored taxa. In the present study, we sought...

The East African species of Parapyrrhicia Brunner von Wattenwyl, 1891, are reviewed. Beside the description of five new species from the West Usambara, Udzungwa, Uluguru Mountains, and coastal Tanzania and the male of P. zanzibarica Brunner von Wattenwyl, 1891, data on habitat, morphology, acoustics, and the chromosomes are provided. A key to the s...

Due to active tectonic evolution of the Aegean Area during Miocene and Pleistocene, the Balkans and Anatolia have repeatedly connected and disconnected causing isolation and secondary contact of populations along the present Dardanelles – Sea of Marmara – Bosphorus waterway. This has led to an outstandingly rich fauna and a reticulate biogeography...

Genus Odontura characterizes with a complicated communication system and low mobility that may have led to an allopatric speciation promoted by geomorphological and climatic factors. Subgenus Odontura comprises 10 of the 12 recognized species of its genus, most distributed in northwestern Africa. Presently, some of its taxa are known from single sp...

Based on faunistic lists documenting the distribution of taxa and molecular data, a phylogenetic tree of African Agraeciini is presented and detailed hypotheses of speciation patterns discussed. It is discussed that the observed radiation in the geologically old Eastern Arc chain is young since Afroanthracites montium endemic to Mts. Kilimanjaro an...

The Plangia graminea complex is partly reviewed and two new species of Plangia are described, Plangia multimaculata n. sp. from savanna habitats and Plangia satiscaerulea n. sp. from the submontane zones in northern Tanzania. Plangia compressa (Walker 1869) is synonymized with P. graminea (Serville, 1838). Data on habitat, bioacoustics, and chromos...

The flightless Agraeciini genus Afroanthracites, a genus restricted to East Africa, is reviewed and two new species are described. Exemplary for the genus Afroanthracites the ecological niche of A. montium from Mt Kilimanjaro is defined. A. montium occupies habitats in humid and perhumid conditions and thus shows a broad altitudinal range (1250-270...

Plant sap-feeding hemipterans harbor obligate symbiotic microorganisms which are responsible for the synthesis of amino acids missing in their diet. In this study, we characterized the obligate symbionts hosted in the body of the xylem-feeding leafhopper Evacanthus interruptus (Cicadellidae: Evacanthinae: Evacanthini) by means of histological, ultr...

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##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

Two orthopteran species belonging to the East African genus Spalacomimus, S. verruciferus and S. talpa (Tettigoniidae: Hetrodinae), were investigated using both molecular cytogenetic techniques, including fluorescence in situ hybridization with 18S rDNA and (TTAGG)(n) telomeric probes, and classical techniques, such as C-banding, fluorochrome CMA(3...

##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

Poecilimon and Isophya are the largest genera of the tribe Barbitistini and among the most systematically complicated and evolutionarily intriguing groups of Palearctic tettigoniids. We examined the genomic organization of 79 taxa with a stable chromosome number using classical (C-banding, silver and fluorochrome staining) and molecular (fluorescen...

We used molecular characters to infer the phylogenetic position of the Western Mediterranean bushcricket genus Odontura and to trace its high karyo-type diversity. Analysis of 1391 base pairs of two mitochondrial genes (COI and ND1) and one nuclear sequence (ITS2) was conducted. Phylogenetic topologies were estimated using maximum parsimony, maximu...

The cytogenetic characteristics of 17 species of bushcricket belonging to eight genera of the tribe Barbitistini were examined by fluorescence in situ hybridization with 18S rDNA and (TTAGGn) telomeric as probes and by C-banding, silver, and fluorochrome staining. These markers were used to understand chromosomal organization and evolutionary relat...

Although Eurycorypha is the most species-rich Phaneropterinae genus in Africa, little is known about the distribution and the ecology of the species. In this study data on distribution, ecology, song and on chromosomes of some East African species are provided. The nymphs of some species are shown, famous for their ant-like appearance and behaviour...

A critical review of the taxonomy and systematics of the genus Isophya from the Balkan Peninsula, together with a revision of the I. modesta group, including its representatives outside the Balkans, has been made using morphological, bioacoustic and karyological data. As a result, the following taxonomic considerations have been proposed: (1) the s...

Chromosomes of six European species (one with two subspecies) of Orthoptera belonging to the tribes Ephippigerini and Bradyporini were analyzed using C-banding, Ag-NOR, DAPI (AT-rich)/CMA3 (GC-rich) staining and fluorescence in situ hybridization (FISH) using the 18S rDNA and (TTAGG)n telomeric probes with the aim to better understand chromosomal o...

Genetic variability between populations of the orthopteran insect Tetrix tenuicornis, collected from six locations in Poland, was assayed by using the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) method. The results show that insects in a population from metal polluted areas in Bolesław have reduced genetic variability in c...

The present study focused on the evolution of the karyotype in 21 taxa of the genus Isophya, which was done by mapping the location on the chromosomes of ribosomal RNA (rRNA) coding genes using fluorescence in situ hybridization (FISH) with an 18S rDNA probe and using silver staining (AgNO3) to evaluate the activity of major rDNA clusters. Since th...

Chromosomes of the males of five species of Odontura, belonging to the subgenera Odontura and Odonturella, were analyzed. Intensive evolution of the karyotype was recorded, both in terms of changes in the numbers of chromosomes (from 2n = 31 to 27) and the sex chromosome system (from X0 to neo-XY and X0 to neo-X1X2Y). Karyotype evolution was accomp...

A preliminary phylogenetic analysis involving 22 taxa of the genus Isophya was conducted using four molecular markers (sequences of mitochondrial cytochrome b (cyt b), fragments of mitochondrial cytochrome oxidase subunit II (COII) and the internal transcribed spacers I and II (ITS1 and ITS2)). Our results indicate that Isophya is a monophyletic gr...

We report the karyotype characteristics including chromosome numbers of Saga campbelli campbelli, S. c. gracilis, and S. rammei using the following classical cytogenetic methods: C-banding, silver staining, and fluorochrome staining DAPI and CMA3. We also present FISH data showing the distribution of telomeric repeats and 18S rDNA on the chromosome...

Podisma sapporensis Shiraki, 1910 (Orthoptera: Acrididae) is distributed on the islands of northern Japan and Far East Russia (Hokkaido, Sakhalin, and Kunashiri), and it exhibits a unique diversity of chromosomal races (X0 and neo-XY) on the island of Hokkaido. To infer the history of geographical isolation and chromosome rearrangements (main races...

Podisma sapporensis Shiraki, 1910 (Orthoptera: Acrididae) is distributed on the islands of northern Japan and Far East Russia (Hokkaido, Sakhalin, and Kunashiri), and it exhibits a unique diversity of chromosomal races (X0 and neo-XY) on the island of Hokkaido. To infer the history of geographical isolation and chromosome rearrangements (main races...

The random amplified polymorphic DNA (RAPD-PCR) method was used to study the genetic polymorphism of 20 species of the genus Isophya. Each primer amplified a different set of DNA fragments, all oligonucleotides failed to generate any specific diagnostic band that could lead to the identification of Isophya species, and none of the amplified fragmen...

The genus Isophya represents one of the largest orthopteran genera with about 45 species occurring in Europe. All over its range the genus includes groups of sibling species, the recognition of which is hampered by their morphological similarity. In part, some species were grouped according to their morphology: the Isophya straubei-group, the I. am...