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Publications (22)
A novel approach for sustained production of therapeutic proteins is described, using genetic modification of intact autologous micro-organ tissue explants from the subject's own skin. The skin-derived micro-organ can be maintained viable ex vivo for extended periods and is transduced with a transgene encoding a desired therapeutic protein, resulti...
Epitope libraries provide an extremely efficient means for epitope mapping and the development of novel diagnostic markers.
Filamentous phage fd-tet expression systems have been developed in order to construct such libraries containing tens to hundreds
of mlllions of random peptlde sequences that can be screened for their abihty to bind particular...
Systemic delivery of therapeutic proteins through gene transfer approaches has been carried out mostly by ex vivo transduction of single cells or by direct in vivo injection of an expression vector. In this work an intact miniature biopsy of human dermis (microdermis) is harvested and transduced ex vivo by a viral vector encoding a gene for the the...
Molecular Therapy (2004) 9, S352–S353; doi: 10.1016/j.ymthe.2004.06.827
921. Biopump: a novel approach to gene-mediated protein production and delivery with application for erythropoietin treatment of anemia
Einat Brill-Almon1, Andrew Pearlman1, Baruch Stern1, Noga Langer1, Daniel Afik1, Menachem Shavit1, Yitzhak Lippin2,*, Amos Panet3,*, Eithan G...
Fimbrial adhesins that mediate attachment to host cells are produced by most virulent Escherichia coli isolates. These virulence factors play an important role in the initial stages of bacterial colonization and also in determination
of the host and tissue specificity. Isolates belonging to serotype O78 are known to cause a large variety of clinica...
Ras genes are the most frequently detected oncogenes in human malignancies. Data regarding the frequency of c-K-ras mutations in esophageal, gastric, and small bowel tumors are limited and controversial.
DNA was extracted from 262 formalin-fixed, paraffin-embedded sections of gastrointestinal samples and tumors, including Barrett's esophagus, esoph...
Several septicemic Escherichia coli O78 strains, isolated from different sources, were characterized phenotypically and genotypically. Two avian isolates, one of which is known to carry the AC/I fimbriae, hybridized with the sfa determinant in colony dot-blot assay. Southern hybridizations with specific sfa probes, following pulsed-field gel electr...
The construction of combinatorial phage-display epitope libraries is a relatively recent innovation. This article describes how such libraries can be used to elucidate protein secondary structures.
Combinatorial phage display peptide libraries are routinely used to map epitopes of specific monoclonal antibodies. In this study we illustrate that these libraries can be used in the analysis of protein structure. By screening libraries at low stringency, a collection of phages can be obtained. These are characterized by the fact that they are rec...
To further our understanding of the nature of HIV-1 immunogenicity, we injected mice with the virus envelope protein gp120 in different configurations: free, complexed with its receptor CD4, and as an immunocomplex with a monoclonal antibody directed against the V3 loop of the protein. Analyses of the polyclonal sera, as well as of monoclonal antib...
The AIDS epidemic has been threatening us for over a decade and despite the enormous research effort, we are still unable to cope with HIV. Neither cure, treatment nor prophylactic-vaccine have emerged that might provide real efficacy against this “stealth virus”.