Bart Van de Vossenberg

Bart Van de Vossenberg
Nederlandse Voedsel- en Warenautoriteit | NVWA · National Reference Centre

PhD

About

68
Publications
20,435
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
844
Citations
Citations since 2017
40 Research Items
654 Citations
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
Introduction
I am the lead molecular biologist of the National Reference Centre for plant health which is part of the Dutch National Plant Protection Organization. We deal with molecular diagnostics and applied research for regulated plant pests and pathogens. Our aim is to act on the forefront of molecular diagnostics and to be a reliable partner in (international) research projects. We also focus on population and genome dynamics for track and trace purposes and the identification of functional markers.
Additional affiliations
January 2012 - August 2019
Nederlandse Voedsel- en Warenautoriteit
Position
  • Researcher

Publications

Publications (68)
Article
Full-text available
Synchytrium endobioticum (Schilb.) Perc. is a chytrid fungus causing potato wart disease and is one of the most important quarantine diseases on cultivated potato. Infected host tissues develop warts rendering the crop unmarketable. Resting spores, that can remain viable and infectious for decades, are formed in warted tissues and are released into...
Article
Full-text available
p>High-throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in a sample. The growing interest in the application of HTS technologies for routine diagnostics in plant health laboratories is triggering the development of guidelines on how to prepare laboratories f...
Article
Full-text available
High-throughput sequencing (HTS) technologies have the potential to become one of the most significant advances in molecular diagnostics. Their use by researchers to detect and characterize plant pathogens and pests has been growing steadily for more than a decade and they are now envisioned as a routine diagnostic test to be deployed by plant pest...
Article
Full-text available
High-throughput sequencing (HTS) technologies have the potential to become one of the most signi cant advances in molecular diagnostics. Their use by researchers to detect and characterize plant pathogens and pests has been growing steadily for more than a decade and they are now envisioned as a routine diagnostic test to be deployed by plant pest...
Article
In the Netherlands, tomato brown rugose fruit virus (ToBRFV; genus Tobamovirus) was first identified in tomato crops in 2019. Since then, the National Plant Protection Organization (NPPO-NL) has performed surveys to track and trace this regulated virus aiming for its eradication. To gain more insight in the epidemiology of ToBRFV, genomes were asse...
Article
Full-text available
Ambrosia beetles of the Euwallacea fornicatus species complex are emerging tree pests with a broad host range including important agricultural crops. Native to Southeast Asia, these species were introduced into various countries, where they cause considerable damage to many tree species. Here we report several outbreaks of E. fornicatus s.l. in Eur...
Article
Full-text available
Nematoda is a diverse phylum and representatives are found in most habitats, including in and on animals and plants. Nematodes are regarded as the most abundant group in terms of individuals in marine and terrestrial sediments. Plant parasitic nematodes are globally responsible for an annual yield loss of $125 billion. Reliable species identificati...
Preprint
Full-text available
Synchytrium endobioticum (Schilb.) Perc. is a chytrid fungus causing potato wart disease and is one of the most important quarantine diseases on cultivated potato. Infected host tissues develop warts rendering the crop unmarketable. Resting spores that can remain viable and infectious for decades are formed in warted tissues which are released into...
Article
Elsinoë species are the causal agents of scab and spot diseases on many economically important plant species. Diagnosis based on symptomology is often problematic and traditional methods are not appropriate as Elsinoë spp. are notoriously difficult to isolate and slow growing. Three Elsinoë species which infect Citrus are regulated as quarantine or...
Article
Full-text available
The Asian tiger mosquito Aedes albopictus is an undesirable invasive mosquito species that causes considerable nuisance through its biting behaviour, and has been proven to transmit more than 22 different viruses under laboratory conditions. Human-aided transportation, the capacity of winter diapause, and possibly global warming have contributed to...
Article
Full-text available
Since 2010, the Centre for Monitoring of Vectors (CMV) of the Netherlands Food and Consumer Product Safety Authority (NVWA), has a surveillance programme in place to detect and control invasive mosquito species (IMS) at locations with increased risk of importation. At the premises of companies that import used tyres from risk areas, the CMV impleme...
Article
Full-text available
Potato wart disease is considered one of the most important quarantine pests for cultivated potato and is caused by the obligate biotrophic chytrid fungus Synchytrium endobioticum. This review integrates observations from early potato wart research and recent molecular, genetic, and genomic studies of the pathogen and its host potato. Taxonomy, epi...
Article
Full-text available
Plots infested with Synchytrium endobioticum, the causal agent of potato wart disease, are scheduled, resulting in prohibition of potato cultivation of and cultivation of plants for planting. On account of robust resting spores that are present in infested soils, plots remain scheduled for 20 years. After this period, plots are intensively sampled...
Article
Full-text available
Rotylenchus is a widely-distributed economically important plant-parasitic nematode group whose species-level identification relies largely on limited morphological characters including character-based tabular keys and molecular data of ribosomal and mitochondrial genes. In this study, a combined morphological and molecular analysis of three popula...
Conference Paper
Full-text available
Genus Longidorus Micoletzky, 1922 comprises ectoparasitic nematode species feeding on plant roots. Some of them have considerable economic importance directly as plant pests or indirectly as vectors of nepo-viruses. During an extensive study on longidorids in Romania Longidorus distinctus Lamberti, Choleva and Agostinelli, 1983 was recovered from v...
Preprint
Full-text available
Ambrosia beetles of the Euwallacea fornicatus species complex are emerging tree pests with a broad host range including important agricultural crops. Native to Southeast Asia, these species were introduced into various countries, where they cause considerable damage to many tree species. Here we report several outbreaks of E. fornicatus s.l. in Eur...
Preprint
Full-text available
Ambrosia beetles of the Euwallacea fornicatus species complex are emerging tree pests with a broad host range including important agricultural crops. Native to Southeast Asia, these species were introduced into various countries, where they cause considerable damage to many tree species. Here we report several outbreaks of E. fornicatus s.l. in Eur...
Article
Full-text available
Background Ticks of the genus Hyalomma , which are vectors for several tick-borne diseases, are occasionally found in areas outside their endemic range including northern parts of Europe. The objective of this study was to analyse adult Hyalomma ticks that were recently found in the Netherlands. Methods Hyalomma ticks were morphologically identifi...
Article
Full-text available
Tomato brown rugose fruit virus (ToBRFV) is a tobamovirus that was first detected in Israel and Jordan following an outbreak of a new disease infecting tomato in 2014. Since then, the virus has been reported from all continents except Oceania and Antarctica. In response to the first finding of the virus in The Netherlands, the Dutch National Plant...
Preprint
Full-text available
Background: Ticks of the genus Hyalomma, which are vectors for several tick-borne diseases, are occasionally found in areas outside their endemic range including northern parts of Europe. The objective of this study was to analyse adult Hyalomma ticks that were recently found in The Netherlands. Methods: Hyalomma ticks were morphologically identifi...
Article
Full-text available
Tomato brown rugose fruit virus (ToBRFV) is a Tobamovirus that was first observed in 2014 and 2015 on tomato plants in Israel and Jordan respectively. Since the first description, the virus has been reported from all continents except Oceania and Antarctica, and has been found infecting both tomato and pepper crops. In October 2019, the Dutch Natio...
Preprint
Full-text available
Tomato brown rugose fruit virus (ToBRFV) is a Tobamovirus that was first observed in 2014 and 2015 on tomato plants in Israel and Jordan causing discolorations and deformation of leaves and fruits. Apart from tomato, damage in pepper fruits has been reported. Since the first description, the virus has been found on all continents except Oceania and...
Article
Full-text available
In Europe, the air-borne accidental introduction of exotic mosquito species (EMS) has been demonstrated using mosquito surveillance schemes at Schiphol International Airport (Amsterdam, The Netherlands). Based upon these findings and given the increasing volume of air transport movements per year, the establishment of EMS after introduction via air...
Article
Full-text available
Potato wart disease is caused by the fungus Synchytrium endobioticum. A workshop on this disease was held in June 2019 at the Dutch National Plant Protection Organization (NPPO‐NL) in Wageningen, the Netherlands. Over 60 participants from 20 countries and 36 organizations came together to share their knowledge via presentations and posters, to stre...
Conference Paper
Full-text available
Potato wart disease is caused by the fungus Synchytrium endobioticum. A workshop on this disease was held in June 2019 at the Dutch National Plant Protection Organization (NPPO-NL) in Wageningen the Netherlands. Over 60 participants from 20 countries and 36 organizations came together to share their knowledge via posters and presentations, to stren...
Article
Full-text available
The pepper weevil, Anthonomus eugenii, is a major pest on Capsicum species. Apart from natural spread, there is a risk of spread via international pepper trade. In the Netherlands, a pepper weevil outbreak occurred in 2012 and affected six greenhouses producing different sweet pepper varieties. The following year, a pepper weevil outbreak occurred...
Article
Full-text available
In June 2019, a single specimen collected at a used tyre company was identified as Aedes flavopictus (Yamada, 1921), a sibling species of Ae. albopictus. Ae. flavopictus has not been recorded outside Japan and South Korea. Although it has only shown dengue virus vector competence under laboratory conditions, its detection demonstrates the value of...
Article
Full-text available
Synchytrium endobioticum is an obligate biotrophic fungus of division Chytridiomycota. It causes potato wart disease, has a worldwide quarantine status and is included on the Health and Human Services and United States Department of Agriculture Select Agent list. S. endobioticum isolates are grouped in pathotypes based on their ability to evade hos...
Article
Full-text available
Synchytrium endobioticum is an obligate biotrophic soilborne Chytridiomycota (chytrid) species that causes potato wart disease, and represents the most basal lineage among the fungal plant pathogens. We have chosen a functional genomics approach exploiting knowledge acquired from other fungal taxa and compared this to several saprobic and pathogeni...
Preprint
Full-text available
Synchytrium endobioticum is an obligate biotrophic fungus of the phylum Chytridiomycota. It causes potato wart disease, has a world-wide quarantine status and is included on the HHS and USDA Select Agent list. S. endobioticum isolates are grouped in pathotypes based on their ability to evade host-resistance in a set of differential potato varieties...
Article
Full-text available
The obligate biotrophic chytrid species Synchytrium endobioticum is the causal agent of potato wart disease. Currently, 39 pathotypes have been described based on their interaction with a differential set of potato varieties. Wart resistance and pathotyping is performed using bioassays in which etiolated tuber sprouts are inoculated. Here, we descr...
Article
In a nature reserve, in Ticino canton in Switzerland amidst a mixed population of non-native Impatiens parviflora and I. glandulifera aberrant plants of I. parviflora were observed. A herbarium voucher and seeds were collected and seedlings subsequently raised in a greenhouse. Molecular analysis for ITS region and chloroplast markers revealed the p...
Article
Full-text available
BACKGROUND: Chytridiomycota species (chytrids) belong to a basal lineage in the fungal kingdom. Inhabiting terrestrial and aquatic environments, most are free-living saprophytes but several species cause important diseases: e.g. Batrachochytrium dendrobatidis, responsible for worldwide amphibian decline; and Synchytrium endobioticum, causing potato...
Preprint
Full-text available
Motivation Numerous tools and databases exist to annotate and interpret the functions encoded in genomes (InterProScan, KEGG, GO etc.). However, analyzing and comparing functionality across a number of genomes, for example of related species, is not trivial. Results We present a novel approach, for which KEGG and Gene Ontology data are imported in...
Article
Full-text available
An international test performance study (TPS) was organised to generate validation data for three molecular Synchytrium endobioticum tests: van den Boogert et al. (European Journal of Plant Pathology 113, 47–57, 2005), and van Gent-Pelzer et al. (European Journal of Plant Pathology, 126, 129-133, 2010) for the detection of S. endobioticum, and the...
Technical Report
Full-text available
DNA barcoding is increasingly used as a diagnostic tool in phytosanitary laboratories. Standardisation of DNA barcoding protocols used in the plant health field and linking them to other barcoding initiatives is essential to benefit from publically available data. DNA barcoding is a generic diagnostic method that uses sequence data of a short stand...
Article
Full-text available
This paper describes a comparison study of test methods and supports the use of real-time polymerase chain reaction (PCR) for the detection of Clavibacter michiganensis subsp. sepedonicus and Ralstonia solanacearum in potato tubers in routine testing. These 2 bacteria are quarantine organisms under European Union (EU) regulatory control and testing...
Technical Report
Full-text available
This Standard provides guidance for the identification of S. littoralis, S. litura, S. frugiperda and S. eridania.
Article
Full-text available
The Old World bollworm, Helicoverpa armigera (Hübner), and the corn earworm, H. zea (Boddie), are two of the most important agricultural pests in the world. Diagnosing these two species is difficult—adults can only be separated with a complex dissection, and larvae cannot be identified to species using morphology, necessitating the use of geographi...
Article
Rapid and reliable tools for detection and identification of plant parasitic nematodes are needed to prevent the introduction and spread of quarantine nematodes. A fast and simple DNA extraction method for target nematodes in nematode suspensions obtained from soil samples and a new quantitative real-time PCR method (qPCR) for the specific detectio...
Article
Full-text available
The European Union (EU) has recently implemented annual surveys in its member States, in order to prevent further introductions of the harmful organism Xylella fastidiosa, within its territory, upon an outbreak in southern Italy where this quarantine pathogen has been identified as the putative agent of the Olive quick decline syndrome (OQDS), (Bos...
Article
Full-text available
Since 2009, The Netherlands Food and Consumer Product Safety Authority carries out surveys focusing on, amongst others, the presence of invasive mosquito species (IMS). Special attention is given to exotic container-breeding Aedes species Aedes aegypti (L.), Aedes albopictus (Skuse), Aedes atropalpus (Coquillett), and Aedes japonicus japonicus (The...
Article
Full-text available
A Banana slug Ariolimax columbianus (Gould, 1851) imported with Salal Summary. In 2013 a Banana slug Ariolimax columbianus (Gould, 1851) was found in a box of Salal Gaultheria shallon imported in the Netherlands. It could be established that banana slugs are regularly imported in the Netherlands with Salal. Two other Ariolimax-specimens that were f...
Article
Full-text available
In the past decade several Phytophthora strains were isolated from diseased Pachysandra terminals plants suffering stem base and root rot, originating from the Netherlands and Belgium. All isolates were homothallic and had a felt-like colony pattern, produced semi-papillate sporangia, globose oogonia and had a maximum growth at ~ 27 C. Several addi...
Article
Full-text available
The genus Spodoptera comprises 31 species, 4 of which are listed as quarantine pests for the European Union: Spodoptera eridania (Cramer), Spodoptera frugiperda (Smith), Spodoptera littoralis (Boisduval), and Spodoptera litura (F.). In international trade, the earlier life stages (eggs and larvae) are being intercepted at point of inspection most f...
Article
Full-text available
In January 2013, a female mosquito collected during the week 18th-25th July 2012 in Lelystad (The Netherlands) during routine national vector surveillance was morphologically identified and genetically confirmed as the Asian bush mosquito, Aedes japonicus japonicus. In order to assess the extent of the infestation area, subsequent extensive mosquit...
Article
Full-text available
Three different versions of the conventional PCR described by Bulman & Marshall (1997) for the identification of cysts and juveniles of Globodera pallida and G. rostochiensis were compared: the original Bulman & Marshall, Bulman & Marshall as described in EPPO PM 7/40 (2) and an in-house modified version of the Dutch National Plant Protection Organ...
Article
Full-text available
The root-knot nematode Meloidogyne ulmi is synonymised with Meloidogyne mali based on morphological and morphometric similarities, common hosts, as well as biochemical similarities at both protein and DNA levels. M. mali was first described in Japan on Malus prunifolia Borkh.; and M. ulmi in Italy on Ulmus chenmoui W.C. Cheng. Morphological and mor...
Article
Full-text available
DNA barcoding protocols for selected EU-regulated arthropods, bacteria, fungi, nematodes and phytoplasmas were developed within the Quarantine organisms Barcoding of Life (QBOL) project financed by 7th framework program of the European Union. DNA barcodes generated with the developed protocols were stored in the Q-bank database. An test performance...
Article
Full-text available
Unlocking the vast genomic diversity stored in natural history collections would create unprecedented opportunities for genome-scale evolutionary, phylogenetic, domestication and population genomic studies. Many researchers have been discouraged from using historical specimens in molecular studies because of both generally limited success of DNA ex...
Data
Percentage, mean and maximum read coverage over all nucleotide positions for chromosomes, scaffolds or linkage groups (LG). (DOCX)
Data
Average nucleotide mis-incorporation rates (substitutions per base) observed in fresh and collection DNA. (DOCX)
Data
Integrity of herbarium DNA. Top, DNA extracts from L. anagyroides fresh (A) and herbarium (B), A. thaliana herbarium (C) and fresh (D), L. tulipifera herbarium (E) and fresh (F), and 1 kb Plus DNA Ladder (Invitrogen), after electrophoresis on 0.8% agarose gels. Bottom, herbarium DNA extracts of A. bisporus (G), P. ostreatus (H) and L. bicolor (I),...
Data
ANOVA on nucleotide mis-incorporation rates for each of the six nucleotide substitution types. (DOCX)
Data
Best BLASTx hits of CDS regions with high number of SNPs or indels. (DOCX)
Data
Genotyping and total number of SNPs and indels in coding sequences (CDS). (DOCX)
Article
Full-text available
In many countries phytosanitary regulations apply to Potato spindle tuber viroid, because it can cause serious diseases in potato and tomato crops. Other pospiviroids, some of which are distributed widely in ornamental crops, can cause similar diseases. Consequently, there is a need for a reliable and cost-effective generic testing method. An assay...
Article
Full-text available
ABSTRACT In this study, six methods for the detection of Phytophthora ramorum in planta were compared using naturally infested rhododendron plant material. The methods included two immunological methods, one an enzyme-linked immunosorbent assay (ELISA) and the other using a lateral flow format (LFD). Three molecular tests based on the polymerase ch...
Article
Full-text available
In this study, six methods for the detection of Phytophthora ramorum in planta were compared using naturally infested rhododendron plant material. The methods included two immunological methods, one an enzyme-linked immunosorbent assay (ELISA) and the other using a lateral flow format (LFD). Three molecular tests based on the polymerase chain react...

Network