Bart A Pannebakker

Bart A Pannebakker
Wageningen University & Research | WUR · Department of Genetics

PhD

About

114
Publications
15,945
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2,291
Citations
Additional affiliations
October 2008 - October 2011
University of Groningen
Position
  • PostDoc Position

Publications

Publications (114)
Article
Full-text available
Biological control (biocontrol) of crop pests is a sustainable alternative to the use of biodiversity and organismal health harming chemical pesticides. Aphids can be biologically controlled with parasitoid wasps, however, variable results of parasitoid‐based aphid biocontrol in greenhouses are reported. Aphids may display genetically encoded (endo...
Article
Full-text available
Insects have a wide variety of mating systems. For parasitic wasps, mating sites are often confined to where their hosts occur and this mutual interaction therefore greatly affects the evolution of alternative mating strategies. Parasitoids of the genus Nasonia (Hymenoptera: Pteromalidae) – comprising the species Nasonia vitripennis (Walker), Nason...
Article
Full-text available
During the transition from sexual to asexual reproduction, a suite of reproduction‐related sexual traits become superfluous, and may be selected against if costly. Female functional virginity refers to asexual females resisting to mate or not fertilizing eggs after mating. These traits appear to be among the first that evolve during transitions fro...
Presentation
Presentation at the Netherlands Annual Ecology Meeting 2021 - NAEM2021. Given on Tuesday, February 9, 2021 in Session 3: Global Change Ecology (note, uploaded before presentation occured). Link to meeting: https://www.nern.nl/naem-2021-programme Presentation files are available at figshare: https://doi.org/10.6084/m9.figshare.13759774.v2 Abstract...
Article
Full-text available
Nesidiocoris tenuis (Reuter) is an efficient predatory biological control agent used throughout the Mediterranean Basin in tomato crops but regarded as a pest in northern European countries. From the family Miridae, it is an economically important insect yet very little is known in terms of genetic information and no genomic or transcriptomic studi...
Article
Full-text available
Bracon brevicornis is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, Ostrinia nubilalis. It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read-based genome of B. brevicornis, complete wi...
Preprint
Full-text available
During the transition from sexual to asexual reproduction, a suite of reproduction-related sexual traits become superfluous, and may be selected against if costly. Female functional virginity refers to asexual females resisting to mate or not fertilizing eggs after mating. These traits appear to be among the first that evolve during the gradual tra...
Article
Full-text available
Biological control is widely successful at controlling pests, but effective biocontrol agents are now more difficult to import from countries of origin due to more restrictive international trade laws (the Nagoya Protocol). Coupled with increasing demand, the efficacy of existing and new biocontrol agents needs to be improved with genetic and genom...
Article
Full-text available
Trichogramma brassicae (Bezdenko) are egg parasitoids that are used throughout the world as biological control agents and in laboratories as model species. Despite this ubiquity, few genetic resources exist beyond COI, ITS2, and RAPD markers. Aided by a Wolbachia infection, a wild-caught strain from Germany was reared for low heterozygosity and seq...
Article
Full-text available
Seasonal polyphenism in Drosophila suzukii manifests itself in two discrete adult mor-photypes, the "winter morph" (WM) and the "summer morph" (SM). These morpho-types are known to differ in thermal stress tolerance, and they co-occur during parts of the year. In this study, we aimed to estimate morph-specific survival and fecundity in laboratory s...
Article
Full-text available
Zoophytophagous predators provide biocontrol services in various major crops of modern horticulture due to the combination of its predatory capacity and the induction of plant defenses derived from its phytophagy. However, under certain conditions of prey scarcity, these natural enemies can inflict plant damage. Exploitation of genetic variation an...
Preprint
Bracon brevicornis is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, Ostrinia nubilalis. It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read genome of B. brevicornis, complete with an...
Preprint
Full-text available
Bracon brevicornis is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, Ostrinia nubilalis . It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read genome of B. brevicornis , complete with a...
Article
Full-text available
Background: Whilst adaptive facultative sex allocation has been widely studied at the phenotypic level across a broad range of organisms, we still know remarkably little about its genetic architecture. Here, we explore the genome-wide basis of sex ratio variation in the parasitoid wasp Nasonia vitripennis, perhaps the best studied organism in term...
Article
Full-text available
The central aim of evolutionary biology is to understand patterns of genetic variation between species and within populations. To quantify the genetic variation underlying intraspecific differences, estimating quantitative genetic parameters of traits is essential. In Pterygota, wing morphology is an important trait affecting flight ability. Moreov...
Preprint
Full-text available
Trichogramma brassicae (Bezdenko) are egg parasitoids that are used throughout the world as biological control agents and in laboratories as model species. Despite this ubiquity, few genetic resources exist beyond COI, ITS2, and RAPD markers. Aided by a Wolbachia infection, a wild-caught strain from Germany was reared for low heterozygosity and seq...
Preprint
Full-text available
Background Whilst adaptive facultative sex allocation has been widely studied at the phenotypic level across a broad range of organisms, we still know remarkably little about its genetic architecture. Here, we explore the genome-wide basis of sex ratio variation in the parasitoid wasp Nasonia vitripennis , perhaps the best studied organism in terms...
Preprint
Full-text available
Nesidiocoris tenuis (Reuter) is an efficient predatory biological control agent used throughout the Mediterranean Basin in tomato crops but regarded as a pest in northern European countries. Belonging to the family Miridae, it is an economically important insect yet very little is known in terms of genetic information: no published genome, populati...
Preprint
Full-text available
The concept of genetic improvement in relation to biological control involves the exploitation of natural genetic variation for the benefit of existing biological control agents (BCAs). Despite recent calls for this process to be adopted in biological control research, there is no clear overview of the current state of research into genetic variati...
Preprint
Full-text available
Biological control is widely successful for controlling pests, but effective biocontrol agents are now more difficult to obtain due to more restrictive international trade laws. Coupled with increasing demand, the efficacy of existing and new biocontrol agents needs to be improved with genetic and genomic approaches. Although they have been underut...
Article
Full-text available
Amblyseius swirskii Athias‐Henriot (Acari: Phytoseiidae) is a predatory mite used to control whiteflies and thrips in protected crops. This biocontrol agent, originating from the Eastern Mediterranean region, has been mass‐reared for commercial use since 2005 and is widely used in augmentative biocontrol programs. As a polyphagous predator, it has...
Article
Full-text available
We previously demonstrated that treatment with the demethylating agent 5-aza-2'-deoxycytidine (5-aza-dC) alters the offspring sex ratios produced by females of the parasitoid wasp Nasonia vitripennis. Females allocate offspring sex ratio in line with local mate competition theory, producing more or less female-biased sex ratios as the number of oth...
Presentation
Full-text available
ABSTRACT: While initially confined to the realm of model species, genome sequencing, assembly, and annotation is becoming more accessible and feasible for de novo projects. There are several uses for the genomes of biocontrol agents, including for the purpose of improvement or risk assessment. Featuring work on biocontrol superstars Trichogramma b...
Presentation
Full-text available
This talk was given at the 2018 ESA/ESC/ESBC Joint Meeting in Vancouver, Canada, at the Member Session, “Rapid evolution in biological control systems: implications for safety and effectiveness” on November 11th, 2018. Speakers notes with extra details are in a separate layer, hover mouse on top left corner. Abstract: While initially confined to...
Article
Full-text available
The mechanisms allowing the widespread invasive pest Drosophila suzukii to survive from early spring until the availability of the first fruit crops are still unclear. Seasonal biology and population dynamics of D. suzukii were investigated in order to better understand the contribution of the early spring hosts to the infestation of the first frui...
Preprint
Full-text available
DNA methylation of cytosine residues across the genome influences how genes and phenotypes are regulated in a wide range of organisms. As such, understanding the role of DNA methylation and other epigenetic mechanisms has become very much a part of mapping genotype to phenotype, a major question in evolutionary biology. Ideally, we would like to ma...
Article
Full-text available
Whole-transcriptome technologies have been widely used in behavioural genetics to identify genes associated with the performance of a behaviour and provide clues to its mechanistic basis. Here, we consider the genetic basis of sex allocation behaviour in the parasitoid wasp Nasonia vitripennis. Female Nasonia facultatively vary their offspring sex...
Poster
Full-text available
Poster presented at Entomologendag 2017 in The Netherlands. Made in the style of a choose your own adventure guide, based on a dichotomous key of sequencing approaches. No original data present, but based on our work so far in sequencing the genomes of different biocontrol agents. CC by 4.0 Abstract: The desire for sequencing non-model insect genom...
Presentation
Full-text available
This is a presentation given the the 5th International Entomophagous Insect Conference in Kyoto, Japan, on October 18th, 2017. Abstract: Innovations within the realm of genetic sequencing have led to increased accessibility for biologists, both in terms of costs as well as the educational resources available, regardless of background. Our project i...
Presentation
Full-text available
Abstract: Biocontrol is the introduction of one organism to control the population of another organism, and has had a commercial application for over a century. With the latest advances in next generation sequencing, now is the moment to both improve biocontrol as well as counter potential shortcomings, such as non-target effects. For example, when...
Article
To recognize one's mate is essential for all sexually reproducing animals. In insects, mate recognition is often based on chemical cues such as hydrocarbons which are distributed over the insect's cuticle. In the parasitoid wasp genus Nasonia (Hymenoptera: Pteromalidae), interspecific mating possibly occurs in microsympatry between Nasonia vitripen...
Article
The hygienic behavior of honey bee workers contributes to the social immunity of colonies. The ability of workers to detect and remove unhealthy or dead brood prevents the transmission of brood diseases inside the colony. Over the last five decades, this trait has been extensively studied and improved in several research and breeding programs. Give...
Article
Full-text available
The parasitoid wasp Melittobia is an important insect for basic and applied biology. Specifically, their extremely female-biased sex ratios, which contrast to the prediction of pre-existing theories, are needed to be explained from the aspect of evolutionary biology. In this study, using next-generation sequencing, 20 microsatellite loci were de...
Article
Full-text available
Intraspecific genetic variation in arthropods is often studied in the context of evolution and ecology. Such knowledge, however, can also be very usefully applied to biological pest control. Selection of genotypes with optimal trait values may be a powerful tool to develop more effective biocontrol agents. Although it has repeatedly been proposed,...
Conference Paper
Full-text available
Introduction: Secure and sustainable food production in terms of quantity and quality is a major challenge facing human societies. However, food security is continuously threatened by current and invasive pest species. Regulations for the use of pesticides are getting stricter to ensure food safety and protect ecosystem health. Biocontrol of agricu...
Article
Full-text available
Linking the evolution of the phenotype to the underlying genotype is a key aim of evolutionary genetics and is crucial to our understanding of how natural selection shapes a trait. Here we consider the genetic basis of sex allocation behaviour in the parasitoid wasp Nasonia vitripennis using a transcriptomics approach. Females allocate offspring se...
Article
Full-text available
Abstract The role of epigenetics in the control and evolution of behavior is being increasingly recognized. Here we test whether DNA methylation influences patterns of adaptive sex allocation in the parasitoid wasp Nasonia vitripennis. Female N. vitripennis allocate offspring sex broadly in line with local mate competition (LMC) theory. However, re...
Article
Full-text available
Sex determination mechanisms are known to be evolutionarily labile but the factors driving transitions in sex determination mechanisms are poorly understood. All insects of the Hymenoptera are haplodiploid, with males normally developing from unfertilized haploid eggs. Under complementary sex determination (CSD), diploid males can be produced from...
Article
Full-text available
Haplodiploidy, where females develop from diploid, fertilized eggs and males from haploid, unfertilized eggs, is abundant in some insect lineages. Some species in these lineages reproduce by thelytoky that is caused by infection with endosymbionts: infected females lay haploid eggs that undergo diploidization and develop into females, while males a...
Data
GO-terms and blast2go analysis for Nasonia vitripennis Nvit2.1 assembly. Files This file - Information and background on methods used. 'Nvit2.1_blast2go_20140711_1551.dat' - blast2go DAT file 'Nvit2.1_GeneSet_Total_LOCs_v1.txt' - Gene set file for GO terms Seq source ftp://ftp.ncbi.nlm.nih.gov/genomes/Nasonia_vitripennis/protein/protein.fa.gz
Article
Full-text available
Trait decay may occur when selective pressures shift, owing to changes in environment or life style, rendering formerly adaptive traits non-functional or even maladaptive. It remains largely unknown if such decay would stem from multiple mutations with small effects or rather involve few loci with major phenotypic effects. Here, we investigate the...
Data
Read depth in the N. vitripennis HVRx outbred laboratory population. Table S1 Microsatellite marker properties. Table S2 Expected heterozygosities per microsatellite marker. Table S3 Heterozygosity in HVRx generation 32 and natural N. vitripennis populations.
Article
Full-text available
The parasitioid wasp genus Nasonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology. To further adva...
Article
Full-text available
Linking behavioural phenotypes to their underlying genotypes is crucial for uncovering the mechanisms that underpin behaviour and for understanding the origins and maintenance of genetic variation in behaviour. Recently, interest has begun to focus on the transcriptome as a route for identifying genes and gene pathways associated with behaviour. Fo...
Data
Full-text available
Proportion of Gene Ontology (GO) terms for biological process (a), molecular function (b) and cellular component (c) in down-regulated tag set (white bars) and in Official Gene Set 2 (black bars) in ovipositing versus resting Nasonia vitripennis females. Significantly enriched terms are indicated with an asterisk. (PDF)
Data
Valid tags used for analysis of digital gene expression between ovipositing and resting Nasonia vitripennis females. Table shows tag identifier (TagID), location of tag in N. vitripennis genome (Nvit 1.0) or Official Gene Set v1.2 (Location) and tag sequence (TagSequence). (XLSX)
Data
Full-text available
Proportion of Gene Ontology (GO) terms for biological process (a), molecular function (b) and cellular component (c) in up-regulated tag set (white bars) and in Official Gene Set 2 (black bars) in ovipositing versus resting Nasonia vitripennis females. (PDF)
Data
Differentially expressed tags in ovipositing versus resting Nasonia vitripennis females. Table shows tag identifier (TagID), tag sequence (TagSequence), mean counts, averaged over all samples from both conditions (baseMean), mean counts from ovipositing females (baseMeanA), mean counts from resting females (baseMeanB), fold change from ovipositing...
Article
Full-text available
An attractive way to improve our understanding of sex determination evolution is to study the underlying mechanisms in closely related species and in a phylogenetic perspective. Hymenopterans are well suited owing to the diverse sex determination mechanisms, including different types of Complementary Sex Determination (CSD) and maternal control sex...
Data
Full-text available
Flow cytometric DNA-histograms of a representative diploid female (a), diploid male (b) and haploid male (c) in A. citri. On the y axis is the number of nuclei, and the x axis is the fluorescence intensity in a log scale, which converts to ploidy in this figure. An excitation wave length of 488 nm and a band pass filter of 585 nm were used to detec...
Data
Comparison of sex ratio (SR), brood size (BS) and pupal mortality (PM) between outcrosses and multiple generations of inbreeding in Asobara tabida, A. japonica, A. citri and A. pleuralis . (DOCX)
Data
Individual-based simulations. (DOCX)
Data
Collection sites and rearing conditions of the four Asobara species used in this study. (DOC)
Article
Nasonia (Hymenoptera: Pteromalidae) are small haplodiploid parasitoids of fleshand blowfly pupae that have become model organisms for speciation research. The genus consists of four closely related species that harbor species-specific Wolbachia bacteria that cause postmating reproductive isolation. Antibiotic curing allows for interspecific crosses...