Barry Saville

Barry Saville
Trent University · Forensic Science Program

BSc, MSc, PhD

About

113
Publications
29,223
Reads
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3,315
Citations
Additional affiliations
July 2020 - present
Trent University
Position
  • Managing Director
July 2019 - July 2020
Trent University
Position
  • Chair
July 2012 - July 2016
Trent University
Position
  • Managing Director
Education
September 1986 - January 1991
University of Toronto
Field of study
  • RNA biochemistry, molecular biology
September 1984 - September 1986
University of Toronto
Field of study
  • Botany/ Molecular Biology
September 1980 - May 1984
University of Guelph
Field of study
  • Botany/ Biochemistry

Publications

Publications (113)
Article
Full-text available
Mercury contamination in aquatic systems poses a serious environmental stress to phototrophic plankton. We used Euglena gracilis to gain an understanding of the physiochemical changes resulting from mercury stress across the transcriptome and metabolome. Using a combination of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS)...
Preprint
teaserEuglenoids show great promise to benefit our world; as biofuels, environmental remediators, anti-cancer agents, robotics design simulators and food nutritional agents, but the absence of reference genomes currently limit realizing these benefits. The Euglena International Network (EIN) (https://euglenanetwork.org/) aims to address these chall...
Article
Full-text available
The emergence of new fungal pathogens threatens sustainable crop production worldwide. One mechanism by which new pathogens may arise is hybridization. To investigate hybridization, the related smut fungi, Ustilago maydis and Sporisorium reilianum, were selected because they both infect Zea mays, can hybridize, and tools are available for their ana...
Article
Full-text available
Smut fungi are a large group of mainly biotrophic plant pathogens, many of which cause disease on cereal crops [...]
Article
As a biotrophic fungus, Ustilago maydis (D.C.) Corda, the causal agent of common smut of corn, must establish and maintain a relationship with the host throughout the pathogenic cycle. Functional characterization of the U. maydis transcription factor, Zfp1, suggests a role in modulating pathogenic development. Deletion of zfp1 resulted in attenuate...
Article
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Reactive oxygen species (ROS) play an important role during host‐pathogen interactions and are often an indication of induced host defense responses. In this study, we demonstrate for the first time that Puccinia triticina (Pt) generates ROS, including superoxide, H2O2, and hydroxyl radicals, during wheat infection. Through pharmacological inhibiti...
Article
Full-text available
Biotrophic basidiomycete plant pathogens cause billions of dollars in losses to cereal crops annually. The model for this group of fungi is the corn smut pathogen Ustilago maydis. Annotation of its genome identified antisense RNAs (asRNAs) complementary to over half of the coded mRNAs, some of which are present at high levels in teliospores but det...
Article
Full-text available
From the evening of March 12, till dinner on March 13, 2017, the 1st International Ustilago/Smut Convergence took place as a workshop prior to the start of the 29th Fungal Genetics Conference, in Asilomar, California. The overall goals of the meeting were to expand the smut model systems being used and to expand participation by the next generation...
Article
Full-text available
Pseudogymnoascus destructans is the causal agent of bat white-nose syndrome (WNS), which is devastating some North American bat populations. Previous transcriptome studies provided insight regarding the molecular mechanisms involved in WNS; however, it is unclear how different environmental parameters could influence pathogenicity. This information...
Article
Smut fungi are the etiological agents of several devastating agricultural diseases. They are characterized by the production of teliospores, which are thick-walled dispersal agents. Teliospores can remain dormant for decades. The dormancy is characterized by low metabolic rates, paused macromolecular biosynthesis and greatly reduced levels of respi...
Article
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Mitigation of emerging infectious diseases that threaten global biodiversity requires an understanding of critical host and pathogen responses to infection. For multihost pathogens where pathogen virulence or host susceptibility is variable, host–pathogen interactions in tolerant species may identify potential avenues for adaptive evolution in rece...
Article
Throughout the plant disease cycle, biotrophic fungal pathogens must obtain host-derived carbon molecules to act as building blocks and sources of energy. Gaining access to these resources requires biotrophic fungi to breach plant cell walls without eliciting substantial plant defences. The plant cell wall is composed mostly of glucose- and xylose-...
Article
Full-text available
Background Biotrophic fungal plant pathogens cause billions of dollars in losses to North American crops annually. The model for functional investigation of these fungi is Ustilago maydis. Its 20.5 Mb annotated genome sequence has been an excellent resource for investigating biotrophic plant pathogenesis. Expressed-sequence tag libraries and microa...
Article
Full-text available
The basidiomycete smut fungus Ustilago maydis causes common smut of corn. This disease is spread through the production of teliospores, which are thick-walled dormant structures characterized by low rates of respiration and metabolism. Teliospores are formed when the fungus grows within the plant, and the morphological steps involved in their forma...
Article
A potential cause of amphibian population declines are the impacts of environmental degradation on tadpole development. We conducted RNA sequencing on developing northern leopard frog tadpoles and through de novo transcriptome assembly we annotated a large number of open reading frames comparable in number and extent to genes identified in Xenopus....
Article
Fungal derived phytohormones play a key role in regulating plant-pathogen interactions however deciphering the separate contribution of the pathogen from the plant during infection has been difficult. Here the Ustilago maydis-Zea mays pathosystem was used to investigate this chemical exchange. U. maydis, the causative agent of corn smut, produces c...
Article
Full-text available
In this study, Ustilago maydis Ndt80 homolog one, unh1, of the obligate sexual pathogen Ustilago maydis,is described. Unh1 is the sole Ndt80-like DNA-binding protein inU. maydis. In this model basidiomycete, Unh1 plays a role in sexual development, influencing tumor maturation, teliospore development and subsequent meiotic completion. Teliospore fo...
Article
Full-text available
Wheat leaf rust, caused by Puccinia triticina Eriks., is of worldwide concern for wheat producers. The disease has been an annual problem for Canadian wheat producers since the early days of wheat cultivation in the 1800s, and research focused on combating this disease began in the early 1900s. Significant progress was made towards understanding th...
Article
Full-text available
Ustilago maydis is the causative agent of common smut of corn. Early studies noted its ability to synthesize phytohormones and, more recently these growth promoting substances were confirmed as cytokinins (CKs). Cytokinins comprise a group of phytohormones commonly associated with actively dividing tissues. Lab analyses identified variation in viru...
Article
Full-text available
Race-specific resistance of wheat to Puccinia graminis f. sp. tritici is primarily posthaustorial and often involves the induction of a hypersensitive response (HR). The aim of this study was to investigate host defense responses induced in interactions between P. graminis f. sp. tritici races and wheat lines carrying different race-specific stem r...
Article
Full-text available
The phytohormones, abscisic acid and cytokinin, once were thought to be present uniquely in plants, but increasing evidence suggests that these hormones are present in a wide variety of organisms. Few studies have examined fungi for the presence of these "plant" hormones or addressed whether their levels differ based on the nutrition mode of the fu...
Article
The sustainable control of basidiomycete biotrophic plant pathogenesis requires an understanding of host responses to infection, as well as the identification and functional analysis of fungal genes involved in disease development. The creation and analysis of a suppressive subtractive hybridization (SSH) cDNA library from Ustilago maydis-infected...
Article
Full-text available
Thatcher near-isogenic lines (NILs) of wheat carrying resistance gene Lr2a, Lr3, LrB or Lr9 were inoculated with Puccinia triticina races of virulence phenotype BBBD, MBDS, SBDG and FBDJ. Puccinia triticina infection structures were analysed under the fluorescence microscope over a course of 14 days after inoculation (dai). The relative proportion...
Article
Full-text available
Ustilago maydis infection of Zea mays leads to the production of thick-walled diploid teliospores that are the dispersal agent for this pathogen. Transcriptome analyses of this model biotrophic basidiomycete fungus identified natural antisense transcripts (NATs) complementary to 247 open reading frames. The U. maydis NAT cDNAs were fully sequenced...
Article
Full-text available
The released genome sequence of Ustilago maydis provided immense insight into this pathogen's genetic structure; however, thorough annotation of the genome requires data from many sources. Information from 4425 expressed sequence tags from the filamentous dikaryon provided an excellent resource for genome annotation. This allowed confirmation and c...
Article
Full-text available
Fungi are models for investigating many eukaryotic molecular processes. The identification of natural antisense transcripts (NATs) in fungi led to the discovery of mechanisms for controlling gene expression through transcriptional interference, chromatin remodelling and dsRNA formation. An overview of these mechanisms and the description of specifi...
Article
Full-text available
Ustilago maydis is the model for investigating basidiomycete biotrophic plant pathogens. To further the annotation of its genome, 12,943 full-length cDNA sequences were used to construct databases for the promoter and untranslated regions of U. maydis genes. A subset of clones was sequenced to determine full cDNA sequences. These and the original E...
Article
Full-text available
The infection of maize (Zea mays) by the basidiomycete fungus Ustilago maydis leads to common smut of corn characterized by the production of tumors in susceptible aboveground plant tissues. LC-(ES)MS/MS profiles of abscisic acid (ABA) and 12 different cytokinins (CKs) were determined for infected and uninfected maize tissues over a time course fol...
Article
Full-text available
Rust fungi are biotrophic basidiomycete plant pathogens that cause major diseases on plants and trees world-wide, affecting agriculture and forestry. Their biotrophic nature precludes many established molecular genetic manipulations and lines of research. The generation of genomic resources for these microbes is leading to novel insights into biolo...
Article
Full-text available
Comprehensive genome annotation requires extensive cDNA analysis. This analysis has identified natural antisense transcripts (NATs), which are distinct from the microRNAs, siRNAs, and piRNAs, in a number of diverse eukaryotes. This wide conservation supports the possibility of an important role for NATs in regulating cellular processes. Investigati...