Barkhutova Darima

• Principal Investigator at Institute of General and Experimental Biology of SB RAS

Microbial diversity in the fecal samples of Bactrian camels in Transbaikalia under various grazing management (free grazing (group I), mixed (group II) and stall housing (group III)) was revealed using high-throughput sequencing of the 16S rRNA gene variable regions. The microbial community of the fecal microbiota was found to be diverse and to dep...

In the vast expanse of Baikal Siberia, indigenous nomadic animal groups have been conserved, grazing on pastures throughout the year. It is believed that the fecal microbiota of these diverse nomadic animal species is unique to each species and closely tied to their feeding environments. We conducted a pioneering comparative analysis of the taxonom...

The Buryatian horse is an ancient breed and, as an indigenous breed, they have unique adaptive abilities to use scarce pastures, graze in winter, and survive in harsh conditions with minimal human care. In this study, fecal microbiota of Buryatian horses grazing in the warm and cold seasons were investigated using NGS technology on the Illumina MiS...

Soil microbial communities play key roles in biogeochemical cycles and greenhouse gas formation during the decomposition of the released organic matter in the thawing permafrost. The aim of our research was to assess the taxonomic prokaryotic diversity in soil-ecological niches of the Darkhituy-Khaimisan transect during the initial period of soil t...

Fermented milk products (FMPs) have numerous health properties, making them an important part of our nutrient budget. Based on traditions, history and geography, there are different preferences and recipes for FMP preparation in distinct regions of the world and Russia in particular. A number of dairy products, both widely occurring and region-spec...

The microbial diversity, transformation potential and ability of microorganisms of the microbial mat consortium of the Kucheger thermal spring (Baikal Rift Zone) to generate electricity were studied. High-throughput sequencing showed the predominance of representatives of the Proteobacteria phylum in the microbial mat community (85.5% of the number...

The Baikal Rift Zone hosts many hot springs with a wide range of temperature and physical-chemical conditions, which may harbour different niches for the distribution of microbial communities. We investigated microbial community composition and their functional activity in two alkaline hot springs with a temperature range of 34.4 to 73.6°C. Compara...

Bacterioplankton community taxonomic composition of four large freshwater lakes of the Yeravninskaya depression (Bolshoye Yeravnoye, Gunda, Isinga, and Sosnovoye) which have great fishery significance for the region has been studied. The microbial community consists in the main of bacterial phyla Gammaproteobacteria (22-74%), Actinobacteria (4-35%)...

We studied freshwater Lake Dikoye located in the coastal zone of Lake Baikal. Negative changes associated with cyanobacterial bloom were observed in the lake. Phototrophs were represented by cyanobacteria, green algae, and diatoms. In the microbial community, Cyanobacteria were the dominant phylum and accounted for up to 48% of the total diversity....

The habitats of colorless sulfur bacteria of the genus Thiothrix have been investigated in 7 hot springs of the Baikal rift zone. The intensity of their development depended on the distance from the outlet of thermal water to the surface and the flow rate of water. The most massive fouling of Thiothrix was observed at the border of mixing thermal a...

The taxonomic diversity of the microbial community of the Baikal rift zone hot springs has been studied. The hot springs were characterized by different temperature (from 34 to 77 C), hydrogen sulfide concentration(from 0.02 to 12.75 mg/L), and silica (from 89 to 144 mg/L). pH values varied from 8.1 to 9.7. The dominance of distinct phyla was revea...

The chemical and microbiological indicators of water were studied in the four large freshwater lakes of the Eravno-Kharginskaya lake system (Buryatia): Isinga, Gunda, Sosnovoe and Bolshoe Eravnoe. These lakes have great fishery and water management significance. The trophication of the lakes and the quality of their waters were evaluated. According...

A draft genome sequence of the bacterial isolate Alg18-2.2, recovered from the highly saline and alkaline lake Gudzhirganskoe (Buryatia, Russia), was determined. The results of bacterial identification using 16S rRNA gene sequence and whole-genome analyses suggest that the bacterium belongs to a novel genus. Some genomic features are discussed here...

The diversity and functional activity of the microbial community of the bottom sediments and water of the thermal alkaline Umkhei Lake (Baikal Rift Zone), which was formed as a result of groundwater discharge, has been studied. The lake water is shown to be thermal hydrocarbonate–sulphate–sodium water of the Kuldur type. Lake sediments are characte...

Alkaline nitrogen thermal springs characterised by high temperatures and pH values are extreme habitats in the Baikal area. Microbial mats are prokaryotic communities consisting of photosynthetic cyanobacteria and a range of other aerobic and anaerobic microorganisms. X-ray diffractometry (XRD) and scanning electron microscopy (SEM) were used to st...

A comprehensive research of water and cyanobacterial mats in Mogoysky and Shurindinsky thermal springs (Baikal rift zone) was carried out by hydrochemical, chemical, microbiological, and mineralogical methods. Detailed descriptions of the springs location and their characteristics were given. According to their chemical composition, the springs wer...

Phototrophic bacterial mats from Kiran soda lake (south-eastern Siberia) were studied using integrated approach including analysis of the ion composition of water, pigments composition, bacterial diversity and the vertical distribution of phototrophic microorganisms in the mats. Bacterial diversity was investigated using microscopic examination, 16...

In this study the bacterial diversity of thermophilic microbial mats (40 to 65°C) in three alkaline hot springs of the Baikal Rift Zone (BRZ) was determined through pyrosequencing of 16S rRNA gene libraries. Significant diversity of bacterial species was found in the biomats of the hot springs with total number of detected phylotypes of 607. The hi...

The prodigious growth of filamentous algae that has been occurring since 2011 in the littoral zone of Lake Baikal poses an acute ecological problem. Samples of algal biomass and aqueous vegetation were collected from the littoral zone of Lake Baikal and converted by hydrothermal treatment into biofuel in the presence of ammonium heptamolybdate. It...

Electrogenic, molecular and some other properties of a microbial mat isolated from the Kuchiger hot spring (Kurumkansky District, Republic of Buryatia) were studied. Molecular analysis showed that representatives of Proteobacteria (85.5 % of the number of classified bacterial sequences) prevailed in the microbial mat of the Kuchiger springs, among...

Alkaline hot springs are unique extreme habitats resemble the early Earth and present a valuable resource for the discovery of procaryotic community diversity and isolation of the novel thermophilic Bacteria and Archaea . One of the model for the possible origin of biochemistry in alkaline hot springs revealed the acetyl-CoA pathway of CO 2 fixatio...

Alkaline hot springs are unique extreme habitats resemble the early Earth and present a valuable resource for the discovery of procaryotic community diversity and isolation of the novel thermophilic Bacteria and Archaea . One of the model for the possible origin of biochemistry in alkaline hot springs revealed the acetyl-CoA pathway of CO 2 fixatio...

Alkaline hydrotherms of the Baikal region are extreme aquatic ecosystems characterized by the presence of the specific microbial communities, mats. A study of the carbohydrate composition of the biomass of microbial maths from four hydrothermal waters of the Baikal region (Alla, Garga, Seya, Umkhei) showed the presence of mannitol (1,93–7,25 mg/g,...

primary destructors in hot springs. The proteolytic bacteria are able to secrete enzymes that are active in wide ranges of pH and temperature. The aim of this work was to study the taxonomic composition, the structure of the bacterial microbial mat, and to study the distribution of peptidases in the thermophilic microbial Garga community. For the s...

Pyrosequencing of the 16S rRNA gene fragments was used to investigate the bacterial community of an Alla hot spring microbial mat. The mat community was mainly represented by the members of five phyla: Deinococcus-Thermus, Nitrospirae, Atribacteria (OP9), Proteobacteria, Chloroflexi, and Firmicutes, with other groups responsible for not more than 2...

Alkaline hydrotherms of the Baikal rift zone are unique systems to study the diversity of thermophilic bacteria. In this study, we present data on the phototrophic bacterial community of cyanobacterial mats from the alkaline Alla hot spring. Using a clonal analysis approach, this study evaluated the species diversity, the proportion of oxygenic and...

Aerobic, facultative anaerobic hydrolytic bacteria are widespread in the sediments and microbial mats of alkaline hot springs in Baikal region (Buryatia). Typical representatives of hydrolytic bacteria are alkalotermophylic bacilli which are capable of utilizing organic matter in alkaline hot spring waters. Two pure cultures of bacteria growing on...

A Gram reaction positive, spore-forming, facultative anaerobic bacterium belonging to the Phylum Firmicutes, was isolated from alkaline hot (80 degrees C, pH 9.8 spring Tsenher, central Mongolia. The cells were rod shaped, feebly motile, peritrichously flagellated. Strain T4 was moderately thermophilic with optimum growth at 60 degrees C. Maximum t...

Hydrochemical and biogeochemical processes in Lake Khilganta were studied during more than ten years. The lake was shown to pass through periods of wetting and drying. This is accompanied by considerable changes in water chemistry and microbiological processes. During the passage from a wet to a dry period, lake water mineralization increases from...

Hoito Gol hot spring of weakly mineralized warm (36–29°C) neutral and partly alkaline nitrogen-carbon dioxide waters belongs to the hydrogeological structure of the actively water-flooded fault marked by the Sentsy River at the southwestern termination of the Baikal rift system. The spatiotemporal evolution of the major parameters of the undergroun...

Two strains of alkaliphilic thermophile bacteria of the genus Anoxybacillus from hydrothermal vents of Lake Baikal were detected and characterized. It was demonstrated that proteinases secreted by these bacteria had wide substrate specificity, hydrolyzed proteins and n-nitroanilide substrates, and showed maximal activity at pyroglutamyl-alanine-ala...

Two strains of alkaliphilic thermophile bacteria of the genus Anoxybacillus from hydrothermal vents of Lake Baikal were detected and characterized. It was demonstrated that proteinases secreted by these bacteria had wide substrate specificity, hydrolyzed proteins and n-nitroanilide substrates, and showed maximal activity at pyroglutamyl-alanine-ala...

Fossil coral reefs provide geological records of late Quaternary sea level changes, tectonic movements and paleoclimate. Most studies on fossil reefs were done on pristine corals that preserve the original aragonite composition and texture. Yet, many fossil corals are affected by various degrees of diagenesis, e.g. recrystallization of aragonite to...

In article minerals formation and elements accumulation in microbial mats of some hot springs of the Barguzin basin (Baikal Rift Zone) is discussed. The content of a wide spectrum of elements in microbial mats is studied by means of the method SR-XRF. Regularity of elements accumulation by community depending on geochemical features of hot spring's...

The structure and functional activity of the microbial communities formed under different environmental conditions of the Khoito-Gol mineral springs are investigated. The habitat of microorganisms in the Khoito-Gol springs is characterized by abundant hydrogen sulfide and intense circulation of sulfur with the participation of sulfate-reducing, thi...

The long-term investigations of structure and biogeochemical activity of phototrophic microbial mats and non-phototrophic biofilms of hydrotherms, freshwater lake, soda and saline lakes located in Baikal rift zone were carried out. Microbial mats, especially phototrophic, are high productive systems with prevalence of production above destruction....

Sulfide and sulfide‐oxide ores of silver‐lead‐zinc deposits (Tarbagatai, Mykert-Sanzheev, and Dovatka) of the Dzhida‐Vitim base metal belt confined to the Paleozoic‐Mesozoic Uda‐Vitim rift zone are characterized by high contents of Ag (up to 2990 g/t), platinum group metals (up to 12.3‐74.6 g/t), and Au (up to 1.2 g/t) [1]. Oxidized ores are more e...

The structure and production characteristics of microbial communities from the Urinskii alkaline hot spring (Buryat Republic, Russia) have been investigated. A distinctive characteristic of this hot spring is the lack of sulfide in the issuing water. The water temperature near the spring vents ranged from 69 to 38.5 degrees C and pH values ranged f...

Results of hydrochemical and microbiological studies of five largest water bodies belonging to the Gusino-Ubukunskaya group are presented. Present-day environmental state of the ecosystem subject to anthropogenic load is described. The possibility of using water quality parameters of the investigated lakes as indicators of the degree of contaminati...

The dominant amino acids (arginine, serine, triptophan, and phenylalanine) of microbial mats of the alkaline Transbaikal Uro and Garga hot springs, the Romanovskii cold mineral spring, and of Khilganta and Verkhnee Beloe soda lakes were determined by thin-layer chromatography. The influence of the mobile phase composition on the selectivity and eff...

Hydrochemical research of the Selenge and its main tributary the Orkhon river on the territory ofMongolia has been conducted. Concentrations of the main water ions were measured. Distribution ofheavy metals was determined. Dynamics of biogenic elements (NO3-, NH4+, phosphates) and degree ofphenol pollution was determined

The microbial mats of different types of springs in Central Asia (Zabaikalye and Mongolia) aredescribed. The species diversity of mat-formed phototrophic bacteria was determined. The rates ofmicrobial destruction processes (sulfate reduction and methane formation) were measured. An importantrole of bacteria and algae in the formation of mineral wat...