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Barbara Pinto

Barbara Pinto
Università di Pisa | UNIPI · Department of Translational Research on New Technologies in Medicine and Surgery

DrPH, & Speciality in Biotechnology

About

62
Publications
11,272
Reads
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1,098
Citations
Additional affiliations
December 2014 - July 2021
Università di Pisa
Position
  • Lecturer
Description
  • Module of Environmental Mutagenesis, Course of Biological Science; Dept. Translational Research NTMS - Lecturer: Safety in the laboratories - CdS "Biomedical laboratory techniques" - Academic year 2018/18ì9 and 2019/20
February 2013 - December 2013
University Hospital of Pisa
Position
  • Lecturer
Description
  • Training Course on Emerging and re-emerging Parasitic Diseases
April 2012 - July 2021
Università di Pisa
Position
  • Lecturer
Description
  • Module of Environmental Mutagenesis, Course of Natural and Environmental Sciences
Education
September 1994 - December 1996
Università di Pisa
Field of study
  • Plant Biotechnologies

Publications

Publications (62)
Article
Matrix metalloproteinases (MMPs), are implicated in the pathogenesis of multiple sclerosis (MS) and in its animal model, experimental autoimmune encephalomyelitis (EAE). Our aim was to investigate whether amelioration of EAE in Dark Agouti (DA) rats, induced by Trichinella spiralis muscle larvae excretory-secretory products (ES L1), could be relate...
Chapter
Enterobiasis, also called oxyuriasis, is caused by the nematode (roundworm) Enterobius vermicularis or pinworm (“small, thread-like worm,” by 1837, from “pin” and “worm”). The infection is one of the most common human parasitic helminthiasis the United States and Western Europe with prevalence rates up to 37.5% even though rate as high as 50% have...
Article
Full-text available
Toxoplasma gondii (TG) is one of the most widespread intracellular parasites in the world, despite the slight declining trend in industrialized countries. Whilst the infection is often asymptomatic in immunocompetent hosts, in immunocompromised patients such as organ transplant recipients it can have important clinical sequels with even fatal conse...
Article
The glutathione-S-transferases omega (GSTO) are multifunctional enzymes involved in cellular defense. During the nurse cell (NC) formation in Trichinella spiralis infection, the structural and regulatory genes of the skeletal muscle cell are downregulated and a new phenotype is acquired which advances parasite growth and survival. Previous studies...
Article
Full-text available
Alternative therapies with new drugs are needed because the clinical efficacy of conventional chemotherapy is often reduced due to collateral effects. Many natural products of plant origin, including essential oils (EOs) have proved to be effective in prevention and therapy of several diseases such as bacterial infections, chronic diseases and canc...
Article
Full-text available
Background Medicinal plant research may contribute to develop new pharmacological control tools for vector borne diseases, such as malaria. Methods The effects of methanol extracts (ME) obtained from seed kernel of ripe and unripe Azadirachta indica fruits were studied on erythrocytic proliferation of the rodent malaria parasite Plasmodium berghei...
Article
Toxoplasmosis has been previously associated with an increased risk of having Schizophrenia or Bipolar disorder in several epidemiological studies. The aim of this observational, cross-sectional study was to examine the seroprevalence of Toxoplasma infection in a cohort of Italian psychiatric inpatients and to verify the presence of circulating Tox...
Article
Full-text available
The aims of this case-control study were to determine the prevalence of Anisakis -specific IgE in patients reporting chronic or acute gastrointestinal (GI) symptoms and to investigate the correlation with raw fish ingestion habits. A group of patients undergoing gastric endoscopy and a control group of healty subjects answered a self-administered q...
Chapter
Matrix metalloproteinases (MMPs) constitute a wide family of more than 20 distinct endopeptidases, either secreted or membrane-bound. They are involved in many physiological (embryogenesis, precursor or stem cell mobilization, tissue remodeling during wound healing, etc.) as well as pathological (inflammation, tumor progression and metastasis in ca...
Article
Full-text available
An accurate estimate of the impact of toxoplasmosis on the population in Italy is not available. We performed a cross-sectional study on individuals living in Italy to assess: (1) differences in access to Toxo testing and in the prevalence of recent and past Toxoplasma gondii infection according to gender and age, and (2) the clinical impact of dis...
Article
Full-text available
Recent evidence suggests the involvement of Toxoplasma gondii infection in the emergence of psychotic and affective disorders. In this report, we describe the case of a young Brazilian woman affected by recurrent ocular toxoplasmosis and presenting with a manic episode with psychotic features in the context of a diagnosis of Bipolar Disorder (BD),...
Article
Matrix Metalloproteinases (MMPs) are involved in many physiological and pathological processes. As regards parasitic infections, the role of these proteins has been particularly studied in malaria, neurocysticercosis and angiostrongyloidosis. Recently, we evaluated serum levels of MMP-9 and -2 (gelatinases) in mice experimentally infected with Tric...
Article
Toxoplasma gondii is a widespread protozoan parasite infecting approximately one third of the world population. After proliferation of tachyzoites during the acute stage, the parasite forms tissue cysts in various anatomical sites including the Central Nervous tissue, and establishes a chronic infection. Clinical spectrum normally ranges from a com...
Article
Trichinella spiralis and Trichinella pseudospiralis exhibit differences in the host-parasite relationship such as the inflammatory response in parasitized muscles. Several studies indicate that matrix metalloproteinases (MMPs) represent a marker of inflammation since they regulate inflammation and immunity. The aim of this study was to evaluate the...
Article
The objective of this in vitro study was to determine the effect of nonylphenol (NP) as an environmental toxicant on the spermatozoa motility and viability. The dose- and time-dependent effect of nonylphenol (1, 10, 100 and 200 μg/mL) dissolved either in 0.1% dimethyl sulfoxide (DMSO) or 0.1% ethanol (ETOH) on the motility and viability of bovine s...
Article
Full-text available
Matrix metalloproteinases (MMPs) represent a large family of over twenty different secreted or membrane-bound endopeptidases, involved in many physiological (embryogenesis, precursor or stem cell mobilization, tissue remodeling during wound healing, etc.), as well as pathological (inflammation, tumor progression and metastasis in cancer, vascular p...
Article
Full-text available
SUMMARY This study aimed to estimate possible changes in seroprevalence of anti-Toxoplasma gondii IgG and IgM antibodies in people living in the area of Massa and Carrara (central Italy), in recent years. Serum samples from over 13 000 individuals were tested for both IgG and IgM anti-Toxoplasma antibodies using an immunoenzymatic method (Access® T...
Article
Full-text available
Toxoplasmosis is a widespread infection, with clinical spectrum ranging from a completely asymptomatic infection to multi-organ involvement. After entering the body, the parasite forms tissue cysts and establishes a chronic infection, involving also the central nervous system (CNS). During the last years, a lot of research has focused on the possib...
Article
Aim. Matrix metalloproteinase(MMP) -2 (MMP-2, gelatinase A) and 9 (MMP-9, gelatinase B) are endopeptidases of the (MMP) family produced by a variety of cells including macrophages and monocytes, that take part in the degradation of extracellular matrix (ECM) and basement membranes. These enzymes are involved in a wide spectrum of both physiological...
Article
Full-text available
This survey aimed to estimate the prevalence of anti-Toxoplasma IgG and IgM antibodies in people living in north west Tuscany (central Italy) and to investigate the adherence to antenatal screening programs and access to the Toxo-test as well. Sera from a large sample of individuals suspected to have acute infection or from pregnant women (10,352 s...
Article
Full-text available
Tomato fruit has assumed the status of 'functional food' due to the association between its consumption and a reduced likelihood of certain types of cancers and CVD. The nutraceutical value of tomatoes can be affected by the cultivation conditions, e.g. the phytochemical content of the fruits may increase with the establishment of beneficial mycorr...
Article
The estrogenic/antiestrogenic activity and the genotoxicity/antigenotoxicity of bee pollen from Salix alba L. and Cystus incanus L. and its derivative extracts in yeast and human cells was investigated. All samples showed a marked inhibitory effect on the activity of the natural estrogen 17 beta-estradiol (higher than 90% for extracts 2) and failed...
Article
High purity water for endocrine disruptors (EDs) analysis in experimental tests is an indispensable requirement for the preparation of reagents and solutions employed in biological laboratories. Commercial ultrapure water may contain traces of organic compounds, which can interfere with in vitro bioassays carried out to detect the potential estroge...
Technical Report
Full-text available
Interferenti endocrini: un modello di test a breve termine in vitro per identificare i possibili rischi per la salute della popolazione connessi ad esposizione a composti estrogenomimetici
Article
Oxidant/antioxidant status, estrogenic/anti-estrogenic activity and gene expression profile were studied in mice fed with Cystus incanus L. (Cistaceae) reach bee pollen from location in Central Croatia's Dalmatia coast and offshore islands. Seven phenolic compounds (out of 13 tested) in bee pollen sample were detected by high performance liquid chr...
Article
Bottled mineral water consumption is steadily rising in the World. Italy is the largest natural mineral water consumer in Western Europe, about 200L per capita per annum. Recently, research has concentrated upon emerging toxicological problems such as the presence in drinking water of substances which interfere with the function of the endocrine sy...
Article
Natural flavonoids are currently receiving much attention because of their estrogenic and antiestrogenic properties. Six isoflavones (isoprunetin, isoprunetin 7-O-beta-D-glucopyranoside, isoprunetin 4',7-di-O-beta-D-glucopyranoside, genistein, genistein 7-O-beta-D-glucopyranoside, daidzein), four flavones (luteolin, luteolin 7-O-beta-D-glucopyranos...
Article
Full-text available
We evaluated the concentration and congener distribution of seven "target" polychlorinated biphenyls (PCBs) present in water collected in different aquaculture farms of the Mediterranean area, commercial feeds, and farmed seafood. PCBs were present in feed and in tissues of all the analysed organisms at levels ranging from 1.96 ng g(-1) to 124.00 n...
Article
Full-text available
A wide variety of environmental pollutants occur in surface waters, including estuarine and marine waters. Many of these contaminants are recognized as endocrine disrupting chemicals (EDCs) which can adversely affect the male and female reproductive system by binding the estrogen receptor and exhibiting hormone-like activities. In this study the es...
Article
Full-text available
A wide variety of environmental pollutants occur in surface waters, including estuarine and marine waters. Many of these contaminants are recognised as endocrine disrupting chemicals (EDCs) which can adversely affect the male and female reproductive system by binding the estrogen receptor and exhibiting hormone-like activities. In this study the es...
Article
Full-text available
Evidence has been presented that a number of environmental agents perturbs the function of the sex hormone signalling pathways in marine animals. In this work the estrogenic/antiestrogenic activity of coastal marine water samples from five sites of the Northern Mediterranean Sea was assayed using a recombinant Saccharomyces cerevisiae yeast strain...
Article
Six isoflavones, daidzein (4',7,-dihydroxyisoflavone), genistein (4',5,7-trihydroxyisoflavone), genistin (genistein 7-O-beta-D-glucopyranoside), isoprunetin (4',7-dihydroxy, 5-metoxyisoflavone), isoprunetin 7-O-beta-D-glucopyranoside, isoprunetin 4',7-di-O-beta-D-glucopyranoside and four flavones, luteolin (3',4',5,7-tetrahydroxyflavone), luteolin...
Article
Full-text available
Monitoring program of hygienic quality water in twelve public swimming pools was performed. Legally required microbiological indicator parameters of safety for gastrointestinal illness were measured besides the analyses of Pseudomonas spp. and Staphylococcus spp. prevalence, frequency of recovery and number of nontuberculous mycobacteria. We detect...
Article
Full-text available
The study investigated the responsiveness of a recombinant Saccharomyces cerevisiae yeast strain to screen estrogen-like chemicals in order to use it as a first level short-term bioassay for environmental preventive medicine purposes. We used the yeast strain RMY326 ER-ERE, containing the human estrogen receptor alpha and a reporter gene lacZ encod...
Article
p> Obiettivi : valutazione del rischio di esposizione della popolazione a composti chimici estrogenomimetici rilasciati da materiali plastici utilizzati nell’industria delle bevande e nel packaging di alimenti Metodi : messa a punto di un metodo di estrazione di composti organici da flussi acquosi tramite l’uso di resine di amberlite® XAD®-2 con p...
Article
Full-text available
The recent revision of the taxonomy of 'faecal streptococci' prompted us to verify the importance of identifying the species of this group of cocci. During a study carried out to assess the hygienic quality of environmental samples from a variety of sources, we isolated 198 strains named faecal streptococci on the basis of conventional internationa...
Article
Full-text available
Densities of mesophilic aeromonads (A. hydrophila, A. caviae, A. sobria) were compared in freshwater rivers in central Italy by means of water quality parameters commonly used to measure the trophic state. Aeromonads concentrations were statistically correlated with phytoplankton community, chlorophyll a concentration, water temperature and recipro...
Article
Full-text available
The frequency of L. monocytogenes and other listerias was determined in different types of Italian-made soft cheeses purchased from retail outlets (shops and supermarkets) located in different areas and different towns of central Italy. Of the 164 examined samples, eight proved to be positive for L. monocytogenes (4.9%), seven strains belonged to s...
Article
Full-text available
In a project to assess the environmental impact of dumping contaminated harbour material in a coastal marine area of the Northern Tyrrhenian Sea, sediments from different sites (harbour, dumping and uncontaminated sites) were sampled in December 1993 and February 1994. In order to evaluate sediment quality, concentrations of heavy metals (Cd, Pb, C...
Article
In cultured mammalian cells, sister chromatid exchanges are easily induced by agents that perturb the scheduled timing of DNA replication. In this work a blockage of DNA synthesis induced by 1-beta-D-arabinofuranosylcytosine was applied to non-tumorigenic and tumorigenic CHEF18 Chinese hamster cells, and their responsiveness was compared. The data...
Article
Full-text available
Doubling time and generation time represent two parameters by which the proliferation of cultured mammalian cells can be monitored. In this study we report the characterization of CHEF18 Chinese hamster cell line during the progression toward tumorigenicity by analysis of doubling time and generation time. The two parameters reveal that the prolife...
Article
Full-text available
Different host-specific bacteriophages were monitored in several water systems together with the usual faecal contamination indicator bacteria (thermotolerant coliforms). The host bacteria used for the plaque assay were E. coli K12, E. coli K12 Hfr, E. coli CHfr and S. typhimurium LT2F+. S. typhimurium LT2F+ showed significantly lower phage counts...
Article
Full-text available
Numerose sostanze chimiche di origine antropica presenti nell'ambiente acquatico possono alterare i modelli di regolazione ormonale negli animali, inclusi gli organismi marini, costituendo un rischio per la biodiversità animale. Sebbene tali sostanze siano presenti in tracce, studi effettuati su pesci del Mediterraneo hanno evidenziato la presenza...

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