Barbara Kot

Barbara Kot
Siedlce University of Natural Sciences and Humanities · Department of Microbiology, Institute of Biology

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63
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1,279
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Publications

Publications (63)
Article
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The effects of physical factors such as radiation (electromagnetic, microwave, infrared, laser, UVC, and X-ray) and high temperature, as well as chemical factors (controlled atmosphere) on the level of global DNA cytosine methylation in C. albicans ATCC 10231 cells were investigated. Prolonged exposure to each type of radiation significantly increa...
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Klebsiella pneumoniae is an important opportunistic pathogen responsible for severe infections, mainly urinary tract infections (UTIs) and pneumonia. Hospital epidemic infections caused by multiresistant strains of carbapenemase-producing K. pneumoniae are the most concerning. NDM-producing strains are resistant to a wide range of antibiotics and h...
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Klebsiella pneumoniae (KP) is a nosocomial pathogen causing difficult-to-treat infections. The presence of virulence genes and antibiotic resistance of 109 KP isolates from hospitalized patients were investigated. Among them, 68.8% were multi-drug resistant (MDR) and 59.6% produced extended‐spectrum beta‐lactamases (ESBLs). Metallo-β-lactamases (MB...
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Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for hard-to-treat infections. The presence of 19 virulence genes in 120 MRSA isolates obtained from hospitalized patients and genetic relationships of these isolates were investigated. The eno (100%) and ebps (93.3%) genes encoding laminin- and elastin binding proteins, respectively,...
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The World Health Organization points out that the opportunistic pathogen Klebsiella pneumoniae that causes various infections among others, urinary tract infections (UTIs), is one of the high-priority species due to a global problem of antimicrobial resistance. The aim of this study was to investigate antibacterial and anti-biofilm activities of ch...
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The effects of two potential antibacterial agents of plant origin: trans-cinnamaldehyde (TC) and thyme oil (TO) on the peripheral blood parameters and cellular composition of hematopoietic tissue of Cyprinus carpio were studied. Both phytochemicals were used in the doses based on the bactericidal concentrations against Aeromonas spp. developed earl...
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The aim of this study was to determine antibiotic resistance patterns and the prevalence of uropathogenes causing urinary tract infections (UTIs) in patients hospitalized in January–June 2020 in central Poland. Antimicrobial susceptibility testing was performed using the disk-diffusion method. Escherichia coli (52.2%), Klebsiella pneumoniae (13.7%)...
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Hematological, biochemical and hematopoietic effects of therapeutic doses of two antibiotics, oxytetracycline (OTC) and gentamicin (GEN), in clinically healthy common carp juveniles were studied. The fish were divided into four groups: controls 1 and 2 (untreated or injected with 0.6% NaCl solution), and two groups treated with antibiotics (orally...
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Methicillin-resistant Staphylococcus aureus (MRSA) are among the most important biofilm-forming pathogens responsible for hard-to-treat infections. Looking for alternatives to antibiotics that prevent biofilm formation, we investigated the effects of manuka honey on the transcriptional profile of genes essential for staphylococcal biofilm formation...
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Antibacterial agents are commonly present in aquatic environment at low concentrations. Terrestrial animal farms, human medicine and aquaculture are main sources of water contamination with antibacterials. Antibiotics were proved to be directly toxic to fish causing oxidative stress, general stress response, histopathological lesions, hematological...
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Easy-to-perform, fast, and inexpensive methods of differentiation of Escherichia coli strains beyond the species level are highly required. Herein two new, original tools for genotyping of E. coli isolates are proposed. The first of the developed method, a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) test uses a hig...
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The effects of trans-cinnamaldehyde (TC) on transcriptional profiles of biofilm-associated genes and the metabolic activity of two methicillin-resistant Staphylococcus aureus (MRSA) strains showing a different degree of adherence to polystyrene, were evaluated. Metabolic activity of S. aureus in biofilm was significantly decreased in the presence o...
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Urinary tract infections (UTIs) belong to the most common community-acquired and nosocomial infections. A main etiological factor of UTIs is uropathogenic Escherichia coli (UPEC). This review describes the current state of knowledge on the resistance of UPEC to antibiotics recommended for the treatment of UTIs based on the available literature data...
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Antimicrobial activities of phytochemicals—trans-cinnamaldehyde (TC), ferulic acid (FA), p-coumaric acid (p-CA), caffeic acid (CA), chlorogenic acid (CHA), Thymus vulgaris essential oil (TO), Eugenia caryophyllus essential oil (ECO), and Melaleuca alternifolia oil (TTO) against Aeromonas species—were assessed. Growth of all Aeromonas salmonicida su...
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Objective: The aim of this study was to determine antimicrobial resistance profiles of methicillin-resistant Staphylococcus aureus (MRSA) isolates from clinical samples from patients hospitalized during 2015 - 2017 in hospitals of Masovian district in Poland. Materials and methods: Antimicrobial resistance of 112 MRSA isolates were tested with a...
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Biofilm-mediated infections in the hospital environment have a significant negative impact on patient health. This study aimed to investigate biofilm production in vitro and the presence of icaABCD genes in methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) strains isolated from hospitalized patients. MRSA (73) and MS...
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The role of genes that are essential for development of Staphylococcus aureus biofilm during infection is not fully known. mRNA from two methicillin-resistant S. aureus strains that formed weak and strong biofilm on polystyrene plates were isolated at five time points from cells grown in biofilm and planktonic culture. Quantitative real-time PCR an...
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Analysis of Lamiaceae essential oils (EOs) by GC-FID-MS revealed the presence as the major constituents of linalool (16.8%), linalyl acetate (15.7%) in Lavandula angustifolia, menthol (29.0%), menthone (22.7%), menthyl acetate (19.2%) in Mentha x piperita, terpinen-4-ol (27.1%), (E)-sabinene hydrate (12.1%), γ-terpinene (10.0%) in Origanum majorana...
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Antibiotic therapy of staphylococcal mastitis is characterized by significantly lower cure rates compared to infections caused by other microorganisms. Thus, it is necessary to search for new, alternative, non-antibiotic agents that are effective in the eradication of these bacteria. The aim of our research was to investigate the antimicrobial, esp...
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The main objective of the study was to analyse the influence of compost produced from urban green waste, sewage sludge from municipal treatment plants and horticultural peat on changes of selected chemical and microbiological parameters of forest soil reclaimed after a fire. Soil samples were collected one and two years after the components had bee...
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The aim of this study was to evaluate the ability of 0.1% thyme oil (TO), trans-cinnamaldehyde (TC), ferulic acid (FA), p-coumaric acid (p-CA), caffeic acid (CA), lavender essential oil (LO), geranium essential oil (GO) and tee tree oil (TTO) to control biofilms formed by methicillin-resistant Staphylococcus aureus (MRSA) strains. Depending on the...
Chapter
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Promising results were also obtained in the investigation of antimicrobial potential of lavender oil (LO). The oil obtained from Lavandula angustifolia demonstrated in vitro activity against MRSA at concentration of <1% [77]. Several chemically characterized lavender oils were assessed for their antibacterial activity using the disc diffusion metho...
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Background/aim: Escherichia coli is the most frequent cause of urinary tract infections. We investigated the possible associations between the origin of strains, antimicrobial resistance, the presence of urovirulence factors, and biofilm-forming ability. Materials and methods: Antibiotic susceptibility of E. coli strains was tested by disk diffu...
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Staphylococcus aureus is arguably the most important pathogen involved in bovine mastitis. The aim of this study was to determine the virulence gene profiles of 124 Staph. aureus isolates from subclinical mastitis in cows in eastern Poland. The presence of 30 virulence genes encoding adhesins, proteases and superantigenic toxins was investigated by...
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Background/aim: Biofilm on urinary catheters results in persistent infections that are resistant to antibiotics. In this study, phytochemicals were assessed as alternative antimicrobials in preventing and inactivating E. coli biofilm on urinary catheters. Materials and methods: Biofilm prevention was tested using catheter fragments inoculated wi...
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The aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with- and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterot...
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The molecular-typing strategy, ERIC-PCR was used in an attempt to determine the genomic relationship of 28 P. aeruginosa strains isolated from faeces of healthy bovine, bovine mastitis and from faeces of hospital patients as well as from environment. ERIC-PCR fingerprinting revealed large molecular differentiation within this group of isolates. Twe...
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The aim of this study was to examine virulence factors and the ability of S. aureus and CNS species isolated from milk of cows with mastitis to form biofilm, and to compare them with virulence factors of staphylococci from milk of cows without mastitis and cowshed environment. Most of S. aureus strains from cows with mastitis showed haemolytic acti...
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The aim of the studies was analysis of resistance of Staphylococcus aureus isolates from bovine mastitis in eastern part of Poland to a set of 20 antibiotics and three alternative agents: lysostaphin, nisin and polymyxin B. Eighty-six out of 123 examined isolates were susceptible to all of 20 tested antibiotics (70%). The highest percentage of resi...
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Atotal of 44 Pseudomonas aeruginosa clinical strains were studied by random amplified polymorphic DNA PCR. (RAPD)-PCR analysis determined the presence of 15 genotypes, with the most frequent genotype A detected in 27.3% of the strains. It was observed that clonally related strains were isolated from patients within the same ward and among different...
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The main problem in the treatment of nosocomial infections is the increasing drug resistance of microorganisms that cause them, limiting the number of effective antibiotics. Pseudomonas aeruginosa bacilli are the cause of many serious hospital-acquired infections occurring primarily in patients within high-risk groups. The most vulnerable are those...
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The aim of this study was to examine phenotypic and genotypic antimicrobial resistance of staphylococci from milk samples from cows with subclinical and clinical mastitis and from cows without mastitis symptoms to methicillin, tetracyclines, macrolides and lincosamides (ML). Of 207 strains, including 34 S. aureus and 173 coagulase-negative staphylo...
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Investigation of antimicrobial resistance and genetic relatedness of staphylococci from milk of cows with mastitis and cowshed environment was the aim of this study. Antimicrobial resistance against 14 antimicrobials were determined by using a disc diffusion method. Genetic similarity between the most frequently isolated species was analysed by PFG...
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A total of 62 Pseudomonas aeruginosa strains isolated from two hospitals in Siedlce (Poland) were studied by repetitive element based PCR (rep-PCR) using BOX primer. BOX-PCR results revealed the presence of 7 numerous genotypes and 31 unique patterns among isolates. Generally, the strains of P. aeruginosa were characterized by resistance to many an...
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This study shows an association between the frequency of the nan1 gene (encoding neuraminidase) among 62 clinical Pseudomonas aeruginosa isolates and adhesion of these bacteria to human buccal epithelial cells. The 52 strains in which the gene was present (83.9%) were characterized by a higher adhesiveness (the mean number of adhering bacteria was...
Article
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A total of 62 Pseudomonas aeruginosa strains isolated from two hospitals in Siedlce (Poland) were studied by repetitive element based PCR (rep-PCR) using BOX primer. BOX-PCR results revealed the presence of 7 numerous genotypes and 31 unique patterns among isolates. Generally, the strains of P. aeruginosa were characterized by resistance to many an...
Article
This study shows an association between the frequency of the nan1 gene (encoding neurami-nidase) among 62 clinical Pseudomonas aeruginosa isolates and adhesion of these bacteria to human buccal epithelial cells. The 52 strains in which the gene was present (83.9%) were characterized by a higher adhesiveness (the mean number of adhering bacteria was...
Article
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In this study, the BOX-PCR fingerprinting technique was evaluated for the discrimination of clinical Pseudomonas aeruginosa isolates. All isolates were typeable and nearly half showed unique banding patterns. According to our results, BOX-PCR fingerprinting is applicable for typing of Pseudomonas aeruginosa isolates and can be considered a useful c...
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Sixty isolates of Yersinia enterocolitica from pigs belonging to 1A and 4 biotypes, and one non-typeable isolate were investigated in order to determine the occurrence of genes directly connected with pathogenicity of Y. enterocolitica by using a multiplex PCR. The multiplex PCR assay was based on the amplification of the ail, ystA, ystB, and yadA...
Article
Yersinia enterocolitica is a pathogen with a global distribution in nature, showing a big diversity in biochemical activity, chemical composition and structure of O-somatic antigen and pathogenic properties. Y. enterocolitica is isolated from animals belonging to different taxonomic groups as well as from food, soil and water, but pigs are consider...
Article
Determination of sensitivity to antibiotics and chemotherapeutics of 160 E. coli strains isolated from 2007 to 2008 from cases of hospital urinary tract infections and assessment the ability to produce ESBL by these strains. The susceptibility of E. coli strains to ampicillin, amikacin, amoxicillin with clavulanic acid, aztreonam, cephalothin, cefo...
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The 150 Y enterocolitica strains isolated from humans and from pigs belonged to biotypes 4 (68.7%), 1A (18.7%) and 2 (4%), or were biochemically untypeable (8.6%). Biotype 4 was comprised of Y. enterocolitica strains representing serotype O:3, within biotype 1A the strains either belonged to serotypes O:5 and O:6 or were untypeable, and biotype 2 w...
Article
Sixty-two isolates of pig-derived Yersinia enterocolitica belonging to 1A and 4 biotypes and non-typeable isolates were subjected to antimicrobial resistance testing by the disc diffusion method. The presence of beta-lactamase A (Bla-A) and B (Bla-B) was detected by the double disc diffusion test, and the beta-lactamase genes blaA and blaB in these...
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The studies were designed to determine an effect of partial replacement of soybean meal with rape cake supplemented with enzymes on the nutritional value of diets and the number of lactic acid bacteria (LAB) in the ileum and caecum of broilers. The experiment 1 (growth trial) was carried out on 120 one-day-old broiler chickens Ross 308 which were r...
Article
A total of 192 pig tonsils were examined for the presence of Yersinia enterocolitica rods. Y. enterocolitica was isolated from 25 (13.02%) pig tonsil samples collected from various herds in Northeast Poland. All isolates, except for one which was not typeable, were found to belong to biotypes 4 (60%) and 1A (36%). The PCR product of 747 bp, resulti...
Article
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A multiplex PCR assay was developed for the detection and differentiation of the Yersinia enterocolitica and Yersinia pseudotuberculosis isolates in both pure bacterial cultures and pig tonsils. The assay was based on the amplification of the ail, inv, yadA, and ystB genes. The PCR products, corresponding to the ail gene and the plasmid-borne yadA...
Article
The interaction of Y. enterocolitica strains belonging to 4 and 1A biotypes with RAW264.7 murine macrophage-like cell monolayers was studied. Y. enterocolitica strains from humans and pigs were characterized in terms of their internalization and survival within RAW264.7 cell monolayers and their ability to escape from the cells. Y. enterocolitica b...
Article
The aim of the study was to determine the adhesion ability and biofilm formation on the surface of a urinary catheter (siliconised latex) by Y. enterocolitica strains representing different biogroups while taking into account their origin. The method used was based on the estimation of the degree of reduction of TTC substrate to insoluble red forma...
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The influence of the presence of bacteriophage ZD5, temperature, pH and the interaction of these factors on survival of Y. enterocolitica cells in pond water and buffers was examined. The presence of bacteriophage ZD5 and temperature have substantial influence on survival of Y. enterocolitica cells in buffers with different pHs. The statistical ana...
Article
The aim of the study was to evaluate hemagglutination properties of Yersinia rods isolated from humans and pigs. Hemagglutination test with 3% fresh and tanned human and sheep group O erythrocytes in the presence and absence of D-mannose was used for detecting the presence and type of fimbriae in these rods. All the tested Y. pseudotuberculosis str...
Article
The subject of this study was thirty nine strains of Yersinia enterocolitica, isolated from faeces of humans who showed symptoms typical of intestinal yersiniosis, and seventy strains of Y enterocolitica, four strains of Y. pseudotuberculosis, and one strain of Y. kristensenii from healthy pigs. In the population tested the following serogroups app...
Article
The study describes the ability of Pseudomonas aeruginosa strains to adhere to buccal epithelial cells and polyurethane (PU) and siliconised latex (SL) catheters. The subject of the study was strains of Pseudomonas aeruginosa isolated from humans (8), hospital sinks (1), cattle (14), swine (6), minks (8), chinchillas (2), fish (26), deer (1), dogs...
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The distribution of different genotypes of Yersinia enterocolitica strains recovered from humans and from healthy pigs was investigated using PCR fingerprinting. The thirty six strains of Y. enterocolitica from humans, thirty five strains from pigs and Y. enterocolitica ATCC 9610 strain were included in this study. The tested strains of Y. enteroco...
Article
The adhesion of Yersinia enterocolitica to type I collagen (pig collagen foil) and pig skin was analysed. Fifteen strains of Y. enterocolitica, including thirteen isolated from humans showing symptoms of intestinal yersiniosis and two strains from pigs, were the subject of the study. The tested strains of Y. enterocolitica 0:3 indicated the ability...
Article
The aim of the present study was to evaluate the lytic activity of three bacteriophages on Yersinia enterocolitica strains isolated from humans and pigs. The Y. enterocolitica strains tested belonged to 0:3, 0:9 and 0:2 serogroups. The ZD5 phage was obtained from a water sample, but remaining phages were obtained from the lysogenic Y. frederiksenii...
Article
One hundred twenty-two strains of Yersinia enterocolitica, isolated from the faeces of humans who showed symptoms typical of intestinal yersiniosis and from pigs, were the subject of the study. The 0:3, 0:9, 0:2, 0:5 serogroups appeared in the tested population of Yersinia enterocolitica. Tested strains were incubated on CRMOX agar and two groups w...
Article
The aim of the investigations was the identification of pathogenic markers of Yersinia enterocolitica isolated from humans and pigs by means of polimerase chain reaction (PCR). Two pairs of the starters Ail-a, Ail-b and Yst-a, Yst-b were used. One hundred twenty-two strains of Yersinia enterocolitica were tested. Among them, 0:3, 0:9, 0:2, 0:5 sero...
Article
The aim of the study was to evaluate the hydrophobic properties of Yersinia enterocolitica and to determine the influence of the culture conditions, such as: type of medium, temperature, and duration of the culture on the manifestation of these properties. The subject of the study were 117 of Y. enterocolitica strains isolated from humans and pigs....
Article
Nine strains of lactic acid bacteria isolated from raw pork and beef were the subject of the study. The antagonistic activity of the tested strains was determined against pathogenic bacteria: Staphylococcus aureus (MRSA and MSSA), enterpathogenic E. coli ad Listeria monocytogenes. A modified Spelhaugh and Harlander method was used. The research was...
Article
The aim of the study was the investigation of bacteriocinogenic properties of 102 Yersinia enterocolitica strains. The influence of selected factors on the production of bacteriocins by Y. enterocolitica and properties of jersiniacin 44JPSBKOH were also investigated. Bacteriocinogenic properties of Y. enterocolitica strains were tested by using the...
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Journal of Polish Agricultural Universities is the very first Polish scientific journal published exclusively on the Internet, founded on January 1, 1998 by the following agricultural universities and higher schools of agriculture: ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION CONCLUSIONS REFERENCES ABSTRACT Evaluation of adherence...

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