Bambang Purwantara

Bambang Purwantara
Bogor Agricultural University | IPB · Department of Veterinary Clinic, Reproduction and Pathology

DVM, MSc, PhD

About

99
Publications
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840
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March 1984 - present
Bogor Agricultural University
Position
  • Professor

Publications

Publications (99)
Article
Full-text available
Madura bulls are Indonesian germplasm with a very high capacity to adapt to dry environments. Madura bulls come from a crossbreed between Zebu ( Bos indicus ) and banteng ( Bos javanicus ). One of the breeding strategies of Madura cattle is the use of artificial insemination (AI) with frozen semen. Regarding sperm motility as one of the standard pa...
Article
Ongole Grade cattle is one of local cattle in Indonesia.This study was aimed to analyze the effect of bull age and season on semen quality of Ongole Grade cattles macroscopically (sperm motility, and sperm concentration) and microscopically (semen ejaculate volume) to support the policy and management on improvement of semen production. Data were c...
Article
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Background and aim: Indonesia has two National Artificial Insemination centers and 17 Regional Artificial Insemination Centers. The frozen semen production techniques differed between the centers, including the type of diluent and semen dilution technique. The aim of the research was to compare the quality of frozen Limousin bull semen diluted usi...
Article
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Bali cattle are one of the native cattle breeds in Indonesia as a meat producer. The objective of this study was to analyze the correlation between body weight and scrotal circumference and sperm quantity of Bali bulls. To accomplish this objective, primary and secondary data of Bali bulls in Baturiti Artificial Insemination (AI) Center, Bali from...
Article
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Friesian Holstein bull (FH) is one of dairy cattle in Indonesia. It is important to increase the production of dairy cattle to meet the need for milk consumption. The way to develop is through artificial insemination (AI). This study was aimed to evaluate the FH bull fertility by calculating the percentage of first service conception rate (%FSCR) o...
Article
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Sericin is a water-soluble globular protein derived from silkworm Bombyx mori and has the competency as an antioxidant. This study was conducted to examine the effect of sericin supplementation in the collection medium on bovine oocyte nuclear maturation. Sericin with different concentration (0 (control), 0.1 %, 0.5 %, and 1%) was added to collecti...
Article
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The mitochondrial genome is able to provide important information for phylogenetic analysis, and evolutionary biology. Therefore, this research aims at sequencing, annotating, and characterizing that of Bubalus quarlesi, which is an endangered endemic species to Sulawesi. Furthermore, it was entirely 16,354 bp in length, consisting of 13 protein-co...
Article
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Aim: This study aimed to analyze the individual factors influencing the sperm quality of Bali bulls at Baturiti Artificial Insemination (AI) center. Materials and methods: Semen that was ejaculated from nine Bali bulls was collected using artificial vaginas (n=5/bull). Semen ejaculates were evaluated immediately after collection to measure the q...
Article
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Background and aim: Holstein cows and heifers are widely bred in Indonesia by artificial insemination (AI) to increase population and milk production. Sperm fertility is modulated by genetic factors, but the analysis of sperm quality is still based on macro- and microscopic characteristics. This study aimed to analyze both sperm quality and protei...
Article
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Background: To obtain accurate measurements of cortisol (C) and testosterone (T) in Aceh cattle, commercial enzyme-linked immunosorbent assay (ELISA) kits need to be carefully validated. Moreover, repeated freeze-thaw cycles during the storage of the samples may affect the stability of the hormones in the serum. Here, the reliability of C and T con...
Article
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Background: To obtain accurate measurements of cortisol (C) and testosterone (T) in Aceh cattle, commercial enzyme-linked immunosorbent assay (ELISA) kits need to be carefully validated. Moreover, repeated freeze-thaw cycles during the storage of the samples may affect the stability of the hormones in the serum. Here, the reliability of C and T con...
Article
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This study aimed to investigate the physical quality of silverside and sirloin meats of aceh cattle, Indonesian beef cattle native to Aceh Province. Meat samples, 250-300 gram each, were collected in triplicate from cull female Aceh cattle have different hair colors slaughtered at the Slaughter House of Banda Aceh. Meat and fat colors, marbling, te...
Article
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Frozen-thawed Belgian Blue (BB) Embryo transfer (TE) is an effort of fulfilling meat demand in Indonesia. This research aimed at observing the correlation between estrus expressions of standing heat and the correlation of CL characteristics on the success rate of frozen-thawed BB embryo transfer. The study was conducted on 55 recipient cattle with...
Article
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Cryopreservation of semen and artificial insemination have an important, positive impact on cattle production, and product quality. Through the use of cryopreserved semen and artificial insemination, sperm from the best breeding bulls can be used to inseminate thousands of cows around the world. Although cryopreservation of bull sperm has advanced...
Article
Full-text available
Background: To obtain accurate measurements of cortisol (C) and testosterone (T) in Aceh cattle, commercial enzyme-linked immunosorbent assay (ELISA) kits need to be carefully validated. Moreover, repeated freeze-thaw cycles during the storage of the samples may affect the stability of the hormones in the serum. Here, we test the reliability of C a...
Article
Full-text available
Goats and sheep are valuable livestock as they produce food, such as meat, milk, fleece, and other products. In addition, goats and sheep are important both for agriculture and biomedical research. Even though these small ruminants provide essential goods, there are major obstacles preventing the efficient, sustainable, and profitable production of...
Article
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Reproduction and development are fascinating processes that exhibit significant differences among mammals. Successful propagation of species depends on male fertility and the ability of the sperm to fertilize and activate the egg and support early development. Remarkably, the specifics of how sperm structure and function have evolved and their cruc...
Article
The aim of this study was to compare the quality of Bali bull fresh semen in productive group (below 10 years old) with over productive group and to evaluate the frozen semen quality of bali bull after cryopreservation in Tris egg yolk extender using Dimethyl formamide (DMF) and glycerol in three different concentrations (5%, 6%, and 7%). Semen was...
Article
The objective of this study was to determine histomorphological change of seminiferous tubules, sperm quality and testosterone level after administration and cessation of pegagan extract. The research used 4x2 factorial complete randomized design, the first factor was dose-administration duration and the second factor was cessation length of pegaga...
Article
Since the non-invasive field endocrinology techniques were developed, several fecal preservation and extraction methods have been established for a variety of species. However, direct adaptation of methods from previous studies for use in crested macaques should be taken with caution. We conducted an experiment to assess the accuracy and stability...
Article
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Background The change in size and weight of the female reproductive organs during gestation and birth might be affect the perineal muscles and this condition in dairy cow not been reported. This study aimed to assess the ultrasonographic image of coccygeus muscle in 11 inseminated dairy cows with different gestational ages and postpartum. Methods...
Conference Paper
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The intensity of the sex skin coloration of crested macaques chan·ges time to time until the time of partus which suggests that the signal can be used for assessing the parturition. Therefore, the objective of the present study was to evaluate the change of sex skin coloration as a reliable signal for assessing the timing of partus and to demonstra...
Article
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The objective of this study was to examine the effects of repeated freeze-thaw cycles and short-term storage of fecal extracts at ambient temperature on the stability of fecal glucocorticoid (fGCM) and estrogen metabolite (fEM) levels from crested macaques.In total 100 aliquots of fecal extracts from fecal samples collected from female crested maca...
Article
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The development of horse breeding industry in Indonesia was commenced through horse racing events held all over the country. It were accelerated by the development of Thoroughbred-Indonesian local Crossbred horses. There are many broodmares injured during their racing time and retired from the racetracks. They may still has a reproductive vigor to...
Article
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Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of Merawang roosters, Kamp...
Article
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Measuring hormone metabolites from feces is the most often used method to assess hormonal status in wildlife. Although immediate freezing of fecal samples collected in the field is the best method to minimize the risk of degradation of hormones over time, this is often not possible in remote field sites. Therefore, alternative storage and preservat...
Article
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Assessments on the steroid hormones profile play an important role in understanding the physiology of species. In the last several decades an alternative method has been developed to monitor steroid hormones through measurements of steroid hormones metabolites excreted in feces and known as a non-invasive method. This method enables continues sampl...
Article
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Cryopreservation of spermatozoa has been used to preserve spermatozoa in very low temperatures. Glycerol is intracelullar cryoprotectant usually used in Tris citrate containing 20% egg yolk (TCEY), while TCEY-1.5% alginate was used as encapsulation media. The effect of alginate in microencapsulation process and glycerol concentration on viability s...
Article
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Microencapsulation of spermatozoa is a process to entrap a number of spermatozoa in microcapsule. Alginate, as a natural polymer polysaccharide is commonly used in cell microencapsulation. Tris Yolk Citrate buffer is a good buffer for spermatozoa dilution, therefore this experiment aimed to determine optimal concentration of alginate and egg yolk t...
Article
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Low quality of sperm after freezing and thawing process due to changes in extreme temperature and osmolarity. The sperm freezing capability and sperm membrane damage was to evaluate by measuring the levels of malondialdehyde (MDA) of Simmental, Limousin and Friesian Holstein (FH) bull of a total of 10 bulls aged 4-8 years. Data were analyzed with a...
Conference Paper
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Orangutans are on the brink of extinction with serious declines in population sizes. Many attempts have been made to protect this species including a reintroduction program as a new approach mandated by the Indonesian government. In the present study we are reporting the assessments of Sumatran orangutan well-being during the rehabilitation and rei...
Article
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This study was conducted to evaluate the effects of sugars supplementation in skim milk based (SM) and dimitropoulos (DV) extenders on the sperm motility and viability in stallion semen storage at 5 oC and ambient temperature (24-29 oC). Semen samples were collected from 3 stallions; evaluate individually and each of them divided into 8 aliquots. F...
Article
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Anoa, which is the endemic animal in Indonesia, its population is unevitably decreasing, therefore anoa included in the list of endangered species by International Union for Conservation of Nature (IUCN). The experiment was aimed to apply the artificial insemination (AI) technique for anoa and to examine their parturition behavior. The experiment i...
Article
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Pregnanediol glucuronide (PdG) in feces and serum progesterone (P) were analyzed for determining 4 ovarian cycle as well as to evaluate ovarian function of captive-housed Javan Gibbon (Hylobates moloch). A total of four females Javan Gibbons maintained in single-typed cage at Ragunan Zoo (Owa1 and Owa2) and in pairing-typed cage at Taman Margasatwa...
Article
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Bali cattle still represents 27% of the total cattle population in Indonesia, and it is considered the pillar breed for small farmers. Moreover, it is a breed of evolutionary importance regarding its direct ancestry from Banteng. However, there is a need for the establishment of a rational system for the evaluation of breeding soundness for indigen...
Article
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Both Bos indicus (zebu) and Bos javanicus (banteng) contribute to the Indonesian indigenous livestock, which is supposedly of a mixed species origin, not by direct breeding but by secondary cross-breeding. Here, the analysis of mitochondrial, Y-chromosomal and microsatellite DNA showed banteng introgression of 10-16% in Indonesian zebu breeds with...
Article
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ABSTRAK Penelitian ini bertujuan untuk menguji fertilitas semen beku rusa timor (Rusa timorensis)menggunakan teknik inseminasi buatan (IB) intraservikal setelah sinkronisasi estrus menggunakan Controlled Internal Drug Release, for Goat (CIDR-G ®). Inseminasi dilakukan 48-60 jam setelah pencabutan CIDR-G ® pada enam ekor rusa timor. Pengamatan melip...
Article
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In order to improve Timor deer (Cervus timorensis) frozen semen quality, the influence of sugar and glycerol concentration on semen characteristics of sperm was investigated. The semen was collected from five sexually mature Timor deer using an electroejaculator. The semen was evaluated and divided into six equal tubes and diluted with Tris sucrose...
Article
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The aim of the study was to produce polyclonal antibody (rabbit anti-ovPAG) which could detect PAG in the urine of pregnant ewes. Twelve rabbits were immunized against ovPG DEAE-TrisHCl (DT), DEAE-NaCl 20mM (DN2), DEAE-NaCl 40mM (DN4), DEAE-NaCl 80mM (DN8), DEAE-NaCl 160mM (DN16), DEAE-NaCl 320mM (DN32) and DEAE-NaCl 1M (DN1) and NaCl 0.9 % as a pl...
Article
Full-text available
The aims of study was to compare the glycerol concentration in Tris glucose egg yolk (TGEY) diluents on the quality of deer frozen semen. Semen was collected from 5 Timor deer using electroejaculator. Immediately after collection the semen was evaluated macroscopic and microscopically. After initial evaluation, the semen was divided into three tube...
Article
Full-text available
In the most species studied sperm abnormalities have long been associated with male infertility and sterility. This study evaluated the sperm morphology (normality and abnormality) of beef cattle at several Artificial Insemination centers in Indonesia. Total of 142 bulls were used in this study; an ejaculate from each bull was examined. A drop of s...
Article
Full-text available
The aims of this study was to compare Tris egg yolk and Citrate egg yolk extender and supplementation of fructose on citrate egg yolk on the quality of Friesian Holstein (FH) bull semen stored at 5 o C. Semen was collected from 5 FH bulls using an artificial vagina. The semen were evaluated macroscopic and microscopically. The semen divided into th...
Article
Full-text available
Anoa is Indonesia endemic fauna that included into Appendix I by CITES. Anoa breeding efforts have not succeed, due to their agressivity, soliter, wildness and monogamous. The aim of this experiment was to observe morphology and biometry of anoa’s sperm stained using William’s (W) and eosin-nigrosin (EN). Semen was collected from two male anoas by...
Article
Full-text available
Morphologically abnormal sperm in semen has been associated with the sub fertility and sterilityfor many years. This study assessed the sperm morphology of Friesian Holstein bull semen which wascollected from three Artificial Insemination centers in Indonesia. Total of 22 bulls were used in thisstudy; an ejaculate from each bull was examined. Three...
Article
Full-text available
Semen used for artificial insemination (AI) can be prepared in different ways, fresh extended fresh or chilled, or frozen-thawed (FT). The aim of the experiment was to evaluate the use of preserved semen to inseminate the oestrus mares. Nineteen mares were used in this research. The mares were synchronized with double injection of PGF2α 14 days apa...
Article
Full-text available
Cryoprotective agents (CPAs) protect sperm during cryopreservation. The objective of this study was to assess different CPAs on stallion semen cryopreservation. Skim milk (SM) and Dimitropoulos (DV) were the extenders used in this study; each was added by glycerol (Gly), combination of ethylene glycol-glycerol (EG+Gly) or dimethilformamide (OMF). E...
Article
Full-text available
Estrone conjugate (E1C) and pregnanediol glucuronide (PdG) were predominant steroid metabolites of estrogen and progesterone in feces of most primates and could be used to evaluate ovarian function. These metabolites were determined along with records of genital swelling throughout 3-4 months period from three female Javan Gibbons (Hylobates moloch...
Article
Full-text available
Semen used for artifi cial insemination (AI) can be prepared in diff erent ways, fresh extended fresh or chilled, or frozen-thawed (FT). The aim of the experiment was to evaluate the use of preserved semen to inseminate the oestrus mares. Nineteen mares were used in this research. The mares were synchronized with double injection of PGF2α 14 days a...
Article
Anoa is Indonesia endemic fauna that included into Appendix I by CITES. Anoa breeding efforts have not succeed, due to their agressivity, soliter, wildness and monogamous. The aim of this experiment was to observe morphology and biometry of anoa's sperm stained using William's (W) and eosin-nigrosin (EN). Semen was collected from two male anoas by...
Article
Full-text available
Molecular detection methods were used to determine if Africanized Honey Bees (AHBs) are present in populations of imported Apis mellifera in Indonesia. The cytochrome b (cyt b gene) was amplified from mitochondrial DNA and digested with the BglII restriction enzyme (cytb/BglII). Two types of animal DNA extraction kits were used and found suitable f...
Article
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The images for Figures 1 and 3 were incorrectly switched. The image that appears as Figure 1 should be Figure 3, and the image that appears as Figure 3 should be Figure 1. The figure legends appear in the correct order.
Article
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This experiment aimed to investigate characteristics of reproductive organ (external parts) of the anoa (Bubalus sp.) and semen characteristics that collected by using electroejaculator after hCG injection, as an initial step on implementation of assisted reproductive techniques. Anoa is Indonesian endemic fauna that included into Appendix I by CIT...
Article
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The objective of this experiment was to evaluate the effects of isoflavone-riched soybean flour, zinc (Zn) and vitamin E on quality of rats spermatozoa as animal model. Diet was given as isonitrogen and isocaloric with 15% of dietary protein. Thirty male Sprague Dawley post weaning rats (21 days old) were divided into six groups and treated with is...