Bambang Irawan

Airlangga University | UNAIR · Department of Biology

The first mitochondrial genome of Ophiocara porocephala was determined by the combination of next-generation sequencing (NGS) and Sanger sequencing methods. A complete circular mitogenome of O. porocephala (16,529 bp) consisted of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and two non-coding regions, including a control region (...

Sea cucumbers have been reported in Surabaya east coastal area for their existence and exploitation. Although Colochirus quadrangularis was one of species which firstly reported from Surabaya waters in 2014, this species has never been properly described. Three specimens of C. quadrangularis were obtained from Surabaya coastal waters. Morphological...

Abstract. The alarming rate of global mangrove forest degradation corroborates the need for providing fast, up-to-date and accurate mangrove maps. Conventional scene by scene image classification approach is inefficient and time consuming. The development of Google Earth Engine (GEE) provides a cloud platform to access and seamlessly process large...

Mangrove forest grows on tropical coastal areas and has an ecological role for its surrounding environment. Mangrove forest protects the coast from large waves and becomes a habitat for various marine fauna. It stores the highest densities of carbon among any other ecosystem globally. In Southeast Asia, Mangrove forest is highly biodiverse and cont...

The objectives of this study were to analyze and compare the effects of mercury (Hg) exposure on osmoregulation and hematological responses in East Java strain tilapia (Oreochromis niloticus). Fish were exposed to 0, 0.1, and 1 mg L⁻¹ Hg at 0, 5, 10, and 15 g L⁻¹ salinities, and serum osmolality (SO), ion level, hematological parameters, and sodium...

Mangroves are very important ecosystems, because of their economic value and environmental services, including as a habitat for various wildlife species, storing carbon, and protecting land from sea abrasion. Indonesia is known to have large mangrove area and diversity. It is estimated that the area of mangroves in Indonesia in 2015 reached about 3...

Research Highlights: This paper provides an alternative approach to contextualize mangrove forest loss by integrating available environmental and socio-economic data sets and products. Background and Objectives: Mangrove forest ecosystems grow in brackish water especially in areas exposed to accumulation of organic matter and tides. This forest typ...

The main objective of this research was to calculate the carrying capacity and growth rate ofisolated Microcystis result in Sutami reservoir on a variety of nitrate and phosphateconcentrations in the B-12 selective medium. Research was conducted in the laboratory withpure experiments using completely randomized factorial design with factors of nitr...

Ticks are one of crucial vectors of disease and parasites of animals. Traditionally, identification of tick species has been conducted using morphological features. This identification method has some limitation such as limited by the condition of samples, little variation among specimens, and difficult to correctly identify the specimens subadult...

Poecilia reticulata is a freshwater fish from the northeastern part of South America and spread widely to various countries in Asia and other continents. However, research about P. reticulate is limited even though it is a well-known fish species in Indonesia. The purpose of study was to identify the fish species of P. reticulata through DNA barcod...

The water shortage has emerged the world since the past decade, it is worsened by the climate change which could affect the hydrological cycle. This condition need to be resolved by finding new sustainable water resource. Sea is dominating 97% of world water resource, yet it has not been explored maximally due to practical and economic issue. Seawa...

DNA barcoding is a new method for identifying species from any biological species using standardized target gen and/or intergenic target. However, identification success is only as good as the underlying database. In this study, cytochrome c oxidase I (COI) sequences of 6 individuals were generated and analyzed from Taphozous melanopogon. Genetic d...

In the present study, we investigated the effects of waterborne copper (Cu) on the levels of metallothionein (MT) and malondialdehyde (MDA), as well as activities of superoxide dismutase (SOD) and catalase (CAT) in gills of cichlid fish Oreochromis niloticus. The Cu concentrations in gills were measured using an atomic absorption spectrometer. The...

The present study reports the presence of metal concentrations (Zn, Cu, Cd, Pb, Hg, and Cr) in the edible tissue of mantis shrimp (Harpiosquilla harpax de Hann, 1844) collected from the eastern region of Java Sea, Indonesia. The concentrations of metals in the muscle tissues of mantis shrimp were determined by atomic absorption spectrometry. The co...

The effects of cadmium concentration (0, 2.5 and 5 mg L⁻¹) on melanocyte-stimulating hormone (MSH), melanophore index (MI), and melanophore number (MN), as well as a microscopic examination of scale melanocytes in tilapia (Oreochromis niloticus Linnaeus, 1757) was evaluated at different salinity levels (0, 5 and 15 ppt). The levels of MSH, MI, and...

The objectives of this study were to determine the length-weight relationships (LWRs), chela length (ChL)-width (ChW) relationships, carapace length (CL)-width (CW) relationships, sexual dimorphism, and condition factor (K) of Cherax gherardiae from Maybrat, West Papua Indonesia. The sex ratio of C. gherardiae was found to be 1.04:1. The LWRs for m...

Recent research conducted in Southeast Asia reveals that mangrove forest degraded 2.12% from 2002 to 2012. Availability of various data products that commonly used to analyze global and regional phenomenon encourages to be utilized for this topic. Therefore, this research aims to utilising specific environment and socioeconomic data products for ex...

Recent research conducted in Southeast Asia reveals that mangrove forest degraded 2.12% from 2002 to 2012. Availability of various data products that commonly used to analyze global and regional phenomenon encourages to be utilized for this topic. Therefore, this research aims to utilising specific environment and socioeconomic data products for ex...

Guppy fish (Poecilia reticulata) is commonly found in various regions and widespread distribution throughout the world, several aquatic environments in Surabaya have been known belong to the category of water quality class III or class IV. Abiotic factors that present in the environment may influence the polymorphism in a guppy fish population. Whe...

Environmental changing conditions impact the diversity of soil as well as the land fill diversity. Different environmental conditions can show different abundances of microorganism. Especially, in Wonorejo Surabaya as mangrove preservation becomes important for ecological monitoring due to a community changing. The mold diversity had been subjected...

Few studies have been conducted concerning the crabs population from the Java Sea. The objective of the present study was to determine the length-weight relationship (LWR), sex ratio, and condition factors (K) of Portunus pelagicus from Java Sea Indonesia. The methods for this study were collecting of sample, estimating of carapace length-weight re...

Measuring individual body length and weight is a basic procedure in fisheries scientific studies. The length-weight relationship (LWR) is a useful tool to predict the weight at a certain length (and vice versa), analyze condition indices, and estimate the biomass of the fishery resources. The objectives of this study were to determine the LWRs, sex...

Effects of cadmium on serum osmolality, ion levels and hematological parameters of tilapia (Orechromis niloticus) were evaluated at different salinities. Serum osmolalities (SOs) in fish unexposed to Cd (0 mg l⁻¹) and exposed to 2.5 mg Cd l⁻¹ were not significantly different at salinities of 0, 5 and 10 g l⁻¹, while at 15 g l⁻¹, SO was significantl...

The objectives of this study were to evaluate the effects of sub-lethal lead concentrations on serum osmolality, Na⁺ and Cl⁻ levels, and hematological parameters in Nile tilapia, Oreochromis niloticus (L.) at different salinity levels. The serum osmolalities (SO) were not significantly different at any of the salinity levels in the control fish, wh...

The objective of present study is to evaluate the impact of different salinities on the levels of CAT, GSH and MDA of the gills of Nile tilapia (Oreochromis niloticus). Nile tilapia was treated by exposure to salinities concentration 0 ‰, 5 ‰ and 10 ‰. Research models were weakened and sacrificed, then took the left and right sides of the gills. Th...

Xestospongia testudinaria Lamarck, 1815 is a marine sponge that become a main constituent in reef ecosystems at northern waters Probolinggo-Situbondo. This barrel sponge species possesses an oxea type of spicule that varies in dimensions (length and width) in concordance to condition and location of habitat. The experiment aimed to understand how s...

The purpose of this study was to determine the quality of the bio-charcoal briquette with materials from leather cassava tubers and sludge of wastewater treatment plant. The first, bio-charcoal briquette analized stability test and compressive strength. Then, bio-charcoal briquette with best value analyzed for parameter including moisture content,...

This study evaluated the effect of different salinity levels on the physiology of East Java strain tilapia (Oreochromis niloticus) by measuring the serum osmolalities (SO), ion levels and hematological parameters. Their SOs above the external medium (hyper-osmotic) at 0 and 5 ppt, becoming iso-osmotic at 10 ppt and hypo-osmotic at 15 ppt. The conce...

The objectives of this study were to investigate the length-weight relationship and condition factor of crayfish living in the natural habitat of South Sorong and Jayawijaya Regencies, Papua, Indonesia. Three species of crayfish (Cherax monticola, C. albertisii and C. communis) were collected from Jayawijaya Regency and only one species of crayfish...

The present study investigated the effects of sublethal concentrations of cadmium (Cd) at different salinity level on the levels of catalase (CAT), glutathione (GSH) and malondialdehyde (MDA) in gills of white shrimp Litopenaeus vannamei. The levels of CAT in gills were significantly higher at salinity 5 than at 15 and 27 with and without Cd exposu...

Species identification and taxonomy of sea cucumber remains a challenge problem in some taxa. Caudinidae family of sea cucumber was comerciallized in Surabaya, and it was used as sea cucumber chips. Members of Caudinid sea cucumber have similiar morphology, so it is hard to identify this sea cucumber only from morphological appearance. DNA barcodin...

This study evaluated the acute toxicity of lead in different life stages of the freshwater prawn Macrobrachium rosenbergii, determined the effect of sublethal Pb concentrations on osmoregulatory capacity (OC), measured the Pb level in gills, and investigated the effect of Pb on the structure of the gills of adult prawns. The 24-, 48-, and 96-h LC50...

This study measured the levels of total mercury (tHg) in the whole tissues of cockles (Anadara granosa and A. antiquata) harvested from three estuaries of Western Lombok Island (WLI), Indonesia. This paper also evaluated the hazard level posed by the mercury in relation to the maximum residual limit for human consumption and to estimate the weekly...

The prawn Macrobrachium sintangense is likely to be subjected to occasional exposure to combined metal and saline stressors in its natural environment. This research evaluated the acute toxicity (96 h LC50) of cadmium (Cd) on the prawn M. sintangense, with respect to the osmoregulatory capacity (OC) of prawns and to document histological changes in...

Contamination of Pb, Cd, Cu, Zn in the flesh on the two types of fish consisting pelagic fish include Black pomfert (Formio niger) whereas among other demersal fish include Tongue sand (Cynoglossus lingua), was conducted in March-December 2013, which was taken from the western part of Madura Strait (Gresik-Ujung pangkah, Surabaya-Kenjeran, and Sido...

The aims of this study are to provide data and information about diversity, dominance, and distribution of the Unionidae mussels species in Brantas River, East Java, Indonesia. Mussel samples were collected from 14 sampling stations along the Brantas River, starting from upstream in Tulungagung to downstream in Surabaya ( ± 170 km) in April-May 201...

Abstract— Birds are one class of Animalia that have high biodiversity in Indonesia, especially water birds (shorebird) as an active migratory birds from the northern hemisphere to the southern hemisphere, and Indonesia is one of the migration paths of traffic and always used as a stopover place while preparing for the journey to the next. One of th...

The objective of this study was to measure the lead, cadmium and mercury levels in the muscle tissues of Leiognathus equulus collected from East Java coast of Indonesia and its impact on human health. All fish collected from these areas present undetectable concentration of mercury. The levels of lead range from 0.002 to 0.007 mg/kg dry weight (d.w...

The objective of this study was to measure the copper (Cu) concentration in gills of juveniles Litopenaeus vannamei after exposure to Cu at sublethal concentrations, and to evaluate its effect upon the structure of gill tissue. The Cu concentration in gills of control shrimp was 0.075 mg/kg. Copper concentrations increased significantly by 147 %, 1...

The purpose of this study was to evaluate the acute toxicity of lead for different life stages of shrimp, Litopenaeus vannamei, and to determine the effect of its sublethal concentrations on osmoregulatory capacity (OC) as well as the possible histological alteration in the gills of juvenile shrimp. The 24-, 48-, and 96-h LC50 values for lead to L....

This study evaluated the acute toxicity of copper at different life stages of freshwater prawn, Macrobrachium rosenbergii, to determine the effect of sublethal copper concentration on osmoregulatory capacity (OC), to measure the copper level in gills, and to investigate the effect of copper on the histological change of the gills of adult prawns. T...

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The objectives of this study were to evaluate the acute toxicity of copper in different life stages of shrimp Litopenaeus vannamei, and to determine the effect of sub-lethal copper concentrations on osmoregulatory capacity. In L. vannamei, the increase of the tolerance to copper is apt with the developmental stage. The 24 h LC50 values were low in...

ABSTRACT Natural wetlands are the natural habitat of waterbirds, but it is very diffi cult to fi nd an unspoiled wetlands without human intervention curently. East coast of Surabaya is wetlands area in Surabaya that most of the region have change into fi shpond and residental. The aims of this reasearch are to determine the community structure of w...

ABSTRACT Diadema setosum is an ecologically important sea urchin which is exists on Bama Coast, but there is no report about the presence of D. setosum in Bama Coast yet. This study was done in order to know the habitat type occurs in Bama Coast, the presence status of D. setosum, distribution pattern, the habitat preference and the character of ha...

This study compared the acute toxicity of Cd and Zn on juvenile Litopenaeus vannamei when administered at different salinities. It also evaluated the effect of sublethal Cd and Zn concentrations on the osmoregulatory capacity (OC) of the juvenile shrimp. Tolerance to Cd and Zn increased progressively with increasing salinity. Cadmium was more toxic...

Bama Beach is one of the most visited beaches in Baluran National Park, Situbondo. However, this beach does not have information of beach profile which is very important for managing coastal and marine resources. The aims of this research are to make beach profiles based on substrate, presence of seagrass, coral lifeforms, and Echinodermata. This r...

The object of this study was to determined the bird species diversity around the estuary region of Kali Lamong located in between Surabaya and Gresik, East Java. Observation was taken three kilometers far, started from Galang Island in Kali Lamong estuary to upstream side of the river. Twelve stations were made along the river as representative typ...

ABSTRACT The object of this study was to determined the bird species diversity around the estuary region of Kali Lamong located in between Surabaya and Gresik, East Java. Observation was taken three kilometers far, started from Galang Island in Kali Lamong estuary to upstream side of the river. Twelve stations were made along the river as represent...

ABSTRACT Bama Beach is one of the most visited beaches in Baluran National Park, Situbondo. However, this beach does not have information of beach profi le which is very important for managing coastal and marine resources. The aims of this research are to make beach profi les based on substrate, presence of seagrass, coral lifeforms, and Echinoderm...

The problem facing in water ecosystem espescially reservoir in Indonesia is the blooming of Microcystis. �he specific objective of this research was to determined the ability of indigenous bacteria formulation from Sutami reservoir, which be known have a capability of reducing nitrate, to controlled Microcystis growth. The research methodology as f...

A survey of the presence of metals in aquatic animals caught from Gresik coastal waters, Indonesia, has been conducted. The results show that all animals contained Zn levels higher than the level of other metals (As, Cd, Cr, Ni, Cu, Se and Pb) in the same species. The copper level recorded in tissue of banana shrimp showed higher copper concentrati...

Microalgae Cyanobacteria from the genus Microcystis has the potential to bloom in Sutami reservoirs that located in Malang, Indonesia at a particular time. Microcystis is a notorious species because it can produce toxins that are dangerous to other organisms. The objective of this research is to determine the influence of different ratios of Nitrat...

The main objective of this research was to calculate the carrying capacity and growth rate of isolated Microcystis result in Sutami reservoir on a variety of nitrate and phosphate concentrations in the B-12 selective medium. Research was conducted in the laboratory with pure experiments using completely randomized factorial design with factors of n...

A study to evaluate total mercury levels in common edible marine animals (the fishes Leiognathus equlus, Coilia dusumieri, Johnius belengerii, Mugil vaigiensis, Arius leptonotacanthus, Chanos chanos, and the shrimp Penaeus merguensis) harvested from Gresik coastal waters, Indonesia, has been carried out during 2004-2008. Concentrations of mercury i...

The problem facing in water ecosystem espescially reservoir in Indonesia is the blooming of Microcystis. The specific objective of this research was to determined the ability of indigenous bacteria formulation from Sutami reservoir, which be known have a capability of reducing nitrate, to controlled Microcystis growth. The research methodology as f...

A survey of the presence of metals in aquatic animals caught from Gresik coastal waters, Indonesia, has been conducted. The results show that all animals contained Zn levels higher than the level of other metals (As, Cd,Cr, Ni, Cu, Se and Pb) in the same species. The copper level recorded in tissue of banana shrimp showed higher copper concentratio...

The study to evaluate the toxicity of used drilling muds (drilling wastes) and their impact on gill structure of the post larvae of tiger prawn Penaeus monodon has been done. The results showed that the 96 h LC50 of used drilling muds ranged from 30740 and 78271 ppm SPP. All toxicity values considered to be above the standard of Indonesian governme...

A monitoring activity for the existence of portunid crabs in the narrowest part of Madura Strait has been done from 2001 to 2004. Crabs have been collected in June or July each year in day time during spring tide. Crab collection was carried out in four sampling station (I to IV) using 0,5 cm trawl net for 10 minutes netting in each time. The resul...

Abstract The present study looks at the aggressive behaviour of male Siamese fighting fish (Betta splendens Regan), which performs four principal elements of agonistic behaviour to visual stimuli, i.e. attack, threat, flight, and submissive. Two elements of this threat behaviour on black and red fish i.e. attack (frontal display/fFD and lateral dis...

The aim of this study was to know the specific expose period of the Lymnaea sp. embyos and their age in order to utilization eggs of Lymnaea sp. in bioassay. Fifty individuals of Lymnaea sp. collected from Gonggan River Magetan were captured in laboratory. Egg cluster of each individual then was placed in petry dish which was filled by Baycarb 500...

Research on the influence of isoniazid on rifampicin metabolism using rat hepatocytes has been done. As the system metabolism, hepatocytes suspension was prepared by perfusion in the calcium binding media containing EDTA, citrate and glycine was used. In the experiment, combination of rifampicin and INH on various doses was incubated in those metab...

Amylase gene (ALP1) insertion in YCp 50 cloning vector was detected by Direct Screening PCR. Result of Direct Screening PCR showed one transformants which assumed have amylase gene insert. Characterization of the recombinant DNA by Stu1 and EcoR1 restriction enzymes indicated nucleotide sequence of insert gene. Digestion by BamHI, Cla1, and EcoRV r...

In the Shigenobu River Estuary (33 ° 48′ 18″ N, 132 ° 41′ 10″ E), Matsuyama (Japan), Helice tridens inhabits the mid-intertidal zone to the limit of the high tide, while H. japonica is found around the mid-intertidal zone. In addition, H. japonica is distributed further downstream than H. tridens. These differences suggest that H. japonica has a lo...

Experiment on salinity tolerance in Macrobrachium sintangense was conducted. The result showed that Macrobrachium sintangense has a wide salinity tolerance and all individuals could survive in the experimental medium up to 20 ppt salinity level, but no individuals could survive in the 35 ppt salinity more than 24 hours.

A survey on the presence of metals in five species of fish and one species of shrimp was conducted in 2003 and 2004 along the Gresik coastal waters, Indonesia. The results showed that almost all metals measured in this study are relatively lower than the values recorded in aquatic animals from other regions of the world. Only the level of zinc was...