
Balaji SeetharamanManipal Academy of Higher Education | MAHE · Department of Biotechnology
Balaji Seetharaman
PhD
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89
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Publications (89)
The chemical, elemental, morphological and toxicological characteristics of organometallic formulation ( Kasthuri Karuppu ) were assessed using UV-spectrophotometer, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, ICP-OES, Energy dispersive X-ray, XRD and Zeta potential. The physical properties such as size (10–80 nm), su...
In the dynamic landscape of targeted therapeutics, drug discovery has pivoted towards understanding underlying disease mechanisms, placing a strong emphasis on molecular perturbations and target identification. This paradigm shift, crucial for drug discovery, is underpinned by big data, a transformative force in the current era. Omics data, charact...
Phytate, an anti-nutritional compound, chelates essential minerals and creates a nutritional deficiency in monogastric animals such as humans. They lack sufficient phytase-producing bacteria in their intestine for phytate degradation. Probiotic bacteria are commonly consumed in humans and can be used as a feasible solution to this problem. Therefor...
Purpose
The textile sector struggles with cotton stickiness from honeydew contamination. It hurts agriculture and marketability. This study aims to examine how bacterial enzymes could reduce honeydew-contaminated cotton adherence in textile businesses sustainably.
Design/methodology/approach
Enzyme was extracted from bacteria isolated from the fer...
New drugs raise regulatory and social concerns about toxicity. Preclinical toxicity investigations require in-silico toxicity prediction since ethics, cost, time, and complexity limit in vitro and in vivo methods. This review discusses the fundamental concepts of biophores and their detection methodologies, tools and techniques, as well as ongoing...
To date, there are very limited reports on sequence analysis and structure-based molecular modeling of phosphatases produced by probiotic bacteria. Therefore, a novel protein tyrosine-like phosphatase was characterized from L. helveticus 2126 in this study. The purified bacterial phosphatase was subjected to mass spectrometric analysis, and the ide...
Chemogenomics, also known as proteochemometrics, covers various computational methods for predicting interactions between related drugs and targets on large-scale data. Chemogenomics is used in the early stages of drug discovery to predict the off-target effects of proteins against therapeutic candidates. This study aims to predict unknown ligand–t...
Purpose
Bales of cotton run through the gins and textile mill instruments, stick to them and make it cumbersome for the ginning mill workers. This is so because more time and money have to be invested in cleaning these instruments. The stickiness of cotton causes health hazards to the workers, decreases the yarn quality and economic loss to the tex...
Introduction:
Deep learning approaches have become popular in recent years in de novo drug design. Generative models for molecule generation and optimization have shown promising results. Molecules trained on different chemical data could regenerate molecules that were similar to the query molecule, thus supporting lead optimization. Recurrent neu...
The 3CL Protease of severe acute respiratory syndrome coronavirus (SARS-CoV), responsible for viral replication, has emerged as an essential target for designing anti-coronaviral inhibitors in drug discovery. In recent years, small molecule and peptidomimetic inhibitors have been used to target the inhibition of SARS-CoV 3CL Protease. In this study...
The present study reports a sustainable source of lignin, from termite frass. Lignin was extracted using Klason’s method and subjected to polarization studies to check the inhibition efficiency and measured the electrochemical performance of the coated sample on the carbon steel using electrochemical impedance spectroscopy. The anticorrosive proper...
Convolutional neural networks (CNNs) have been used to extract information from various datasets of different dimensions. This approach has led to accurate interpretations in several subfields of biological research, like pharmacogenomics, addressing issues previously faced by other computational methods. With the rising attention for personalized...
The role of translocases was underappreciated and was not included as a separate class in the enzyme commission until August 2018. The recent research interests in proteomics of orphan enzymes, ionomics, and metallomics along with high throughput sequencing technologies generated overwhelming data and revamped this enzyme into a separate class. Thi...
The word “bamboo” reminds us of “a hollow stick,” but it is filled with a plethora of health benefits. The tribals of northeastern India ferment these beneficial bamboo shoots for the goodness of mankind. Fermentation is an important age-old biotechnological procedure used for the preservation of food products. Fermented bamboo shoots form the nich...
With millions of smart devices being integrated each day through the Internet of Things (IoT), ensuring ubiquitous computing along with pertinent security has now become more significant than ever. Generally, it is difficult to guarantee zero downtime even if the backhaul network is deployed optimally. But, an automated system that could predict th...
The study aims to characterize and understand the toxicological effects of colloidal mercuric formulation. The physiochemical characterization was carried out using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), Energy dispersive X-ray microanalysis system (EDS), Inductively coupled plasma optical emission s...
Although, 187 years elapsed after the discovery of melezitose, it is a high time to deduce some solid applications as there are only 13 more years left to celebrate a double century of this sugar. The forgotten sugar has multifarious applications; it is used as a metabolic marker to differentiate melezitose fermenting microorganisms, as a carbon so...
The aggressive multiplication of coronavirus in the Indian population is probably due to the faster mutation rate. At the time of commencement of this work, India was not present in the list of Top 10 worst-affected countries. However, upon completion of this manuscript, India is ranked No. 3 and during publication of this manuscript it may even el...
Phytate-linked nutritional deficiency disorders have plagued poultry for centuries. The application of exogenous phytases in poultry feed has served as a solution to this problem. However, they are linked to certain limitations which include thermal instability during prolonged feed processing. Therefore, in this study, Streptococcus thermophilus 2...
Predicting the strength of binding affinity between compounds and proteins with reasonable accuracy is crucial in drug discovery. Computational prediction of binding affinity between compounds and targets greatly enhances the probability of finding lead compounds by reducing the number of wet-lab experiments. Machine-learning and deep-learning tech...
Computational methods have been widely used in drug discovery including identification of novel targets, studying drug target interactions, and in virtual screening of compounds against known targets. Machine learning techniques have been used in predictions of novel targets and drugs with greater accuracy compared to other methods. Machine learnin...
There are extensive research works on HIV-1 genome and its encoded proteins. The genome comprises of nine genes that code for at least 15 proteins. Although these sequences are available to the public, the structure information is incomplete. The structure information is vital to understand the pathogenesis as well as for preventive measures. There...
Global Virology, Volume III: Virology in the 21st Century examines work that has been undertaken, or is planned, in several fields of virology, in an effort to promote current and future work, research, and health. Fields and methods addressed include virology, immunology, space research, astrovirology/astrobiology, plasmids, swarm intelligence, bi...
An in vitro simulated chicken gut model was proposed for studying the phytase activity of selected bacteria such as Streptococcus thermophilus, Sporosarcina pasteurii, Sporosarcina globispora, and Sporosarcina psychrophila using known probiotic bacterium, Lactobacillus helveticus as a control. The selected bacteria were viable in the intestinal lum...
Tetherin, an interferon-induced host protein encoded by the bone marrow stromal antigen 2 (BST2/CD317/HM1.24) gene, is involved in obstructing the release of many retroviruses and other enveloped viruses by cross-linking the budding virus particles to the cell surface. This activity is antagonized in the case of human immunodeficiency virus (HIV)-1...
Probiotics are well known for their wide range of beneficial activities. However, recent use of probiotic Bifidobacterium, Enterococcus, and Lactobacillus spp. has been plagued by certain disadvantages such as complex growth requirements, high maintenance cost, susceptibility to the gastrointestinal environment, pathogenic gene transfer, non-standa...
[This corrects the article DOI: 10.1371/journal.pone.0195800.].
Hydrophobic-Polar model is a simplified representation of Protein Structure Prediction (PSP) problem. However, even with the HP model, the PSP problem remains NP-complete. This work proposes a systematic and problem specific design for operators of the evolutionary program which hybrids with local search hill climbing, to efficiently explore the se...
Microbial degradation of myo-inositol hexakisphosphate (IP6) is crucial to deal with nutritional problems in monogastric animals as well as to prevent environmental phosphate pollution. The present study deals with the degradation of IP6 by microorganisms such as Sporosarcina spp. pasteurii, globiospora, psychrophila, Streptococcus thermophilus and...
Background
The present study is aimed to evaluate the physiochemical properties and cytotoxicity of mercury-based formulation for the development of anticancer therapeuticals.
Methods
The elemental and morphological features of the formulation were characterized by FE-SEM, XPS and EDS. The described formulation was evaluated for its cytotoxicity o...
The prediction of the tertiary structure of a protein from its primary sequence is a long-standing problem
for life science researchers. Solutions to this problem have a direct impact on other research areas such as design and
discovery of new drugs, understanding the sequenced genomic data, solving the puzzle of protein structure, and understandin...
Injection drug use is a major risk behavior associated with transmission of HIV-1B. Yet, despite its importance there have not been many detailed studies characterizing the transmission of HIV-1B in well-defined injection drug use networks. This preliminary study characterized people who were closely associated and injected drugs together under pri...
In order to understand the structure and folding of proteins, Hydrophobic-Polar (HP) model on 2D square lattice is one of the most explored models but parity problem of square lattice make it inefficient for biological applications. This work is dedicated to solve parity issues in 2D square lattice model. This work proposes a revised energy functio...
Significance and impact of the study:
4-Methylumbelliferone (4-MU) is a widely used chloretic and is currently under research for treating colon cancer. Preliminary studies suggest that it has the potential to be used as an effective and sustainable prebiotic for the human microbiome, as it can be naturally obtained from plants. This manuscript de...
Introduction
The escalating threat due to dwindling effect of antibiotics and challenge of tackling rising drug-resistant infections has gathered high focus in current medicinal research.
Methods
In an attempt to find new molecules that can defeat microbial resistance, two new series of 2-[2-substituted ethenyl]-5-(substituted methoxy)-1,3,4-oxadi...
Probiotics play a vital role in clinical applications for the treatment of diarrhea, obesity and urinary tract infections. Phytate, an anti-nutrient, chelates essential minerals that are vital for human health. In the past few decades, research reports emphasize extensively on phytate degradation in animals. There is a growing need for finding alte...
Background:
The traditional Indian medicine 'Siddha' uses metals, metalloids and minerals including toxic ones with no proven toxicity. Thalagak karuppu (TK) is remarkably stable over a century and used for treating Suram (Fever), Kaasam (Cough), Elai (Tuberculosis) and Eraippu Erumal (Bronchial Asthma).
Objective:
The present study addresses el...
The largest digital repository of information, the World Wide Web keeps growing exponentially and calls for data mining services to provide tailored web experiences. This chapter discusses the overview of information retrieval, knowledge discovery and data mining. It reviews the different stages of data mining and introduces the wide spread biologi...
Inositol hexakisphosphate is known to be the phosphorous reserve in plants particularly in the seeds. Although it has been known for its anti-nutrient properties for many years, recent research shed light on its anticancer properties. Hence, the present study focuses on probable protein targets of phytic acid (PA) through computational methods. Ant...
Inositol hexakisphosphate is known to be the phosphorous reserve in plants particularly in the seeds. Although it has been known for its anti-nutrient properties for many years, recent research shed light on its anticancer properties. Hence, the present study focuses on probable protein targets of phytic acid (PA) through computational methods. Ant...
Graphics Processing Unit (GPU) accelerated computing has pioneered a new direction of research for various combinatorial optimization problems. One such problem which requires huge computation is Protein Structure Prediction (PSP). PSP is NP-complete problem. Computational prediction of protein native structure from its primary amino acid sequence...
Background and Objective: The Protein Structure Prediction (PSP) problem is one of the hardest problems in computational biology and technological research. To reduce the complexity of the problem, Dill proposed the Hydrophobic-Polar (HP) model which subsequently became a major tactic to the PSP problem. Methodology: In this study, a novel algorith...
Ab initio protein structure prediction (PSP) models tertiary structures of proteins from its sequence. This is one of the most important and challenging problems in bioinformatics. In the last five decades, many algorithmic approaches have been made to solve the PSP problem. However, it remains unsolvable even for proteins of short sequence. In thi...
Schistosomiasis is an endemic neglected tropical disease caused by trematodes of the genus Schistosoma. Purine nucleoside phosphorylase (PNP) of purine salvage pathway is essential for recovery of nucleosides in Schistosoma species. Schistosoma mansoni PNP (SmPNP) is well-explored drug target for schistosomiasis. The currently available drugs have...
Inositol hexakisphosphate is known to be the phosphorous reserve in plants particularly in the seeds. Though it has been known for its antinutrient properties for many years, recent research shed light to reveal it as a novel anticancer agent. Hence the present study investigates the drug-likeness of phytic acid and its analogues through bioinforma...
The first part of this chapter introduces the proteins encoded by HIV-1. Their consensus sequences, structures, and functions are discussed. Amino acid sequence variations due to different selective pressures that drive the evolution of HIV-1 including compartmentalization and geographical variations are addressed. Sequence and phylogenetic analysi...
On 14 October 2010, the WHO reported that more than 1 billion people worldwide who live in remote rural areas are affected by neglected tropical diseases. Leishmaniasis is caused by protozoa of more than 20 different species in humans. The three major forms of disease are cutaneous, mucocutaneous and visceral leishmaniasis (VL). Cutaneous leishmani...
Bioinspiration through biological nanomachines and their assemblies at molecular level provide guiding principles to build nanoscale functional devices. The rapid advances in the biosynthesis of nanomaterials, including hybrid nanomaterials and their applications are reviewed. Biological synthesis of nanostructures using whole organism as well as b...
Colorectal cancer is the third leading cause of cancer related deaths in humans. Research is going on to increase the treatment options of colon cancer with decreased side effects. Four imidazolidinones were synthesized by the cyclization reaction between Schiff bases and ethylchloroacetate and were characterized by advanced spectral techniques and...
The essential oil extracted by hydro-distillation from rhizome of Curcuma caesia Roxb. grown in southern India were analyzed by gas chromatography and gas chromatography–mass spectrometry. A total of 35 volatile essential oil components were identified in the extracted oil of C. caesia representing tropolone (15.86 %) as major compound, ledol (3.27...
Research Article (E)-1-(2,3-dimethoxyphenyl)-N-(4-methylpyridin-2-yl)methanimineas a potent anticancer agent against colorectal cancer
The author's biomorphic presentations of proteins are intended to promote the understanding of protein structure by analogy with living organisms. The understanding of a protein's structure is necessary to the understanding of its function. The analogies discussed in this paper can be used to understand the structure-function relationship; they con...
Any detailed discussion of protein function inevitably involves protein structure. In this article, a visual representation intended to allow the artistic interpretation of protein structure data is presented. The authors have simplified the representation of proteins through the use of the traditional South Indian folk art kolam. This artistic rep...
Any detailed discussion of protein function inevitably involves protein structure. In this article, a visual representation intended to allow the artistic interpretation of protein structure data is presented. The authors have simplified the representation of proteins through the use of the traditional South Indian folk art kolam. This artistic rep...
Extensive work has been done to explore the potential promises of plant systems in the synthesis of metal nanoparticles. We report here a green approach for the synthesis of silver nanoparticles with a cocktail of biochemical constituents from cashew apple juice (Anacardium occidentale L.). We presume that the reduction potential of these constitue...
Extensive work has been done to explore the potential promises of plant systems in the synthesis of metal nanoparticles. Herein, a convenient and rapid process for the formation of silver nanoparticles with natural reducing and capping agents of Daucus carrota (L.) is reported. The synchronization of secondary metabolites and enzymes as reducing/ca...
Polymers from renewable resources recently attracted research community mainly because of environmental concerns, low cost, availability and their biodegradability after usage. However, the intrinsic deficiencies of natural biopolymers, including poor mechanical properties, poor water resistance and difficult processability limit their use. The ana...
A general type of molecular level metabolic control that is common to all living organisms is the feedback inhibition, wherein an increase in the product of a metabolic pathway regulates an enzyme upstream in the cascade by binding to it and decreasing enzyme activity. The de novo pyrimidine biosynthetic pathway provides a specific example for the...
Large number of papers has been published recently on the eleventh group metallic elements such as Ag, Au and Cu. Our study was focused on biosynthesis of silver nanoparticles, their morphology, reactivity and stability. We were interested to check these properties in two different samples, S1 and S2, respectively. The biosynthesis of silver nanopa...
An elevated level of blood uric acid (hyperuricemia) is the underlying cause of gout. Xanthine oxidase is the key enzyme that catalyzes the oxidation of hypoxanthine to xanthine and then to uric acid. Allopurinol, a widely used xanthine oxidase inhibitor is the most commonly used drug to treat gout. However, a small but significant portion of the p...
Turmeric belongs to the ginger family Zingiberaceae. Currently, cheminformatics approaches are not employed in any of the spices to study the medicinal properties traditionally attributed to them. The aim of this study is to find the most efficacious molecule which does not have any toxic effects. In the present study, toxicity of 200 chemical comp...
The recognition of gene/protein names in literature is one of the pivotal steps in the processing of biological literatures for information extraction
or data mining. We have compiled a lexicon of biomedical words (conserved patterns/ potential motifs) which has the combination of only 20
alphabets of amino acids. The remaining 6 letters of the Eng...
Plant chitinases have been of particular interest since they are known to be induced upon pathogen invasion. Inoculation of Piper colubrinum leaves with the foot rot fungus, Phytophthora capsici leads to increase in chitinase activity. A marked increase in chitinase activity in the inoculated leaves was observed, with the maximum activity after 60...
Cheminformatics approaches are currently not employed in any of the spices to study the medicinal properties traditionally attributed to them. The aim of this study is to find the most efficacious molecule which do not have toxic effects but at the same time have desired pharmacokinetic profile. In the present study of the class 'diphenylheptanoids...
This book (24 chapters) covers the chemistry (chemical composition and structure) of the following spice plants and their products, and provides brief information on the morphology, and postharvest management (storage, packaging and grading) of these crops: black pepper ( Piper nigrum ), small cardamom ( Elettaria cardamomum ), large cardamom ( Amo...
Alkaline shock proteins (ASPs) adapt to atypical conditions, which are categorized into a family of small proteins. Alkaline shock proteins are identified in many bacteria and they are involved in stress response. The molecular basis for the survival of bacteria under extreme conditions in which growth is inhibited is a question of great current in...
The protein based phylogenetic trees based on 21 isolates of Cucumber mosaic virus estimated for 1a, 2a, 3a and 3b proteins, suggest that the 3a movement protein (MP) and 3b coat protein (CP) were congruent and indicated no significant difference (p-value= 0.699 > 0.05) in the branch lengths. The conservation might be due to the coordination of CP...
The molecular basis for the survival of bacteria under extreme conditions in which growth is inhibited is a question of great current interest. A preliminary study was carried
out to determine residue pattern conservation among the antiporters of enteric bacteria, responsible for extreme acid sensitivity especially in Escherichia coli and
Shigell...
The molecular basis for the survival of bacteria under extreme conditions in which growth is inhibited is a question of great current interest. A preliminary study was carried out to determine residue pattern conservation among the antiporters of enteric bacteria, responsible for extreme acid sensitivity especially in Escherichia coli and Shigella...
The ability to calculate the correct sequence alignment is crucial to many types of studies. The accuracy in alignment is
critical in predicting gene ancestry, the number and location of point mutations, evolutionary distance and phylogeny. A
study was conducted to test the biological significance of PIR pairwise alignment using 40 N-terminal signa...
Obtaining structural information about Vif is of interest for several reasons that include the study of the interaction of Vif with
APOBEC3G, a resistance factor. Vif is a potential drug target and its function is essential for the HIV-1 infectivity process. To
study Vif mechanism of action, we need to decipher its structure. Pivotal in this appr...
Obtaining structural information about Vif is of interest for several reasons that include the study of the interaction of Vif with APOBEC3G, a resistance factor. Vif is a potential drug target and its function is essential for the HIV-1 infectivity process. To study Vif mechanism of action, we need to decipher its structure. Pivotal in this approa...
In silico approaches are currently not employed in any of the spices to study the toxicity. The aim of this study is to find the most efficacious molecule which does not have any adverse effects. In the present study one hundred and eight compounds from cardamom were used to predict mutagenicity and carcinogenecity. The results of these studies ind...
To evaluate the effects from the fresh flowers of Pithecellobium dulce (Roxb.) Benth, belonging to the family of Leguminosae subfamily Mimosoideae, a glycoside quercitin has been isolated. The ethyl acetate soluble of P. dulce containing the above glycoside was studied both in silico and in vitro for the anti-inflammatory and anti-bacterial propert...