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B. Valdazo-González

B. Valdazo-González
Medivet Laboratory Services Ltd · Molecular Biology

PhD, DVM

About

41
Publications
7,249
Reads
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930
Citations
Additional affiliations
March 2020 - June 2020
Medivet
Position
  • Medical Professional
Description
  • Real time PCR of infectious diseases in dogs and cats, including the detection of coronaviruses and parvoviruses.
February 2015 - February 2020
Medicines and Healthcare products Regulatory Agency (MHRA)
Position
  • PostDoc Position
Description
  • Poliovirus mutations using Mutant Analysis by PCR and Restriction Enzyme Cleavage (MAPREC) and next generation sequencing (NGS). Detection of viruses causing acute flaccid paralisis using metagenomics. Detection of viruses in sewage using NGS.
July 2009 - February 2015
The Pirbright Institute
Position
  • PostDoc Position
Description
  • Key scientist in the following DEFRA-funded research projects: - Molecular epidemiology of foot-and-mouth disease virus; - Development of forensic tracing tools for foot-and-mouth disease virus in the world using full-genome sequencing.
Education
May 2001 - June 2009
Universidad de León
Field of study
  • Pestivirus infection in small ruminants:clinical and epidemiological signs and genetic and antigenic characterization of the virus
September 1995 - June 2000
Universidad de León
Field of study
  • Animal Health

Publications

Publications (41)
Article
Full-text available
Background: Next-Generation Sequencing (NGS) is revolutionizing molecular epidemiology by providing new approaches to undertake whole genome sequencing (WGS) in diagnostic settings for a variety of human and veterinary pathogens. Previous sequencing protocols have been subject to biases such as those encountered during PCR amplification and cell c...
Article
Full-text available
Foot-and-mouth disease virus (FMDV) is a highly contagious and genetically variable virus. Sporadic introductions of this virus into FMD-free countries may cause outbreaks with devastating consequences. In 2010 and 2011, incursions of the FMDV O/SEA/Mya-98 strain, normally restricted to countries in mainland Southeast Asia, caused extensive outbrea...
Article
Full-text available
Improvements to sequencing protocols and the development of computational phylogenetics have opened up opportunities to study the rapid evolution of RNA viruses in real time. In practical terms, these results can be combined with field data in order to reconstruct spatiotemporal scenarios that describe the origin and transmission pathways of viruse...
Article
A real time one-step RT-PCR was designed to detect and type border disease virus (BDV), bovine viral diarrhea virus (BVDV) type 1 and BVDV type 2 in ovine samples. The real time RT-PCR was shown to behave in a linear manner and had limits of detection of 100-1000 copies of viral RNA as judged by in vitro transcribed RNA. The real time RT-PCR was va...
Article
Full-text available
A selection of 10 pestiviruses isolated from sheep from the Iberian Peninsula from 2001 to 2004 was characterised at the molecular level. The 5' untranslated region (5'-UTR) and N(pro)-coding gene were amplified by the reverse transcription-polymerase chain reaction (RT-PCR) and sequenced directly from purified products. All isolates were also type...
Article
Full-text available
Introduction: Standardisation is important across the life cycle of medicinal products, supporting the diagnosis, treatment and prevention of a wide range of diseases. In particular, reference materials have a crucial role in helping to harmonise data across assays, standardise results between labs, and develop medicinal/diagnostic products or vacc...
Article
Aims Human Sapoviruses (HSaVs) are etiological agents of sporadic cases and outbreaks of acute gastroenteritis in humans of all ages. Evidence of worldwide distribution of HSaV has been documented, however little is known about HSaV circulation in Italy. To study their occurrence and genetic diversity a nation‐wide environmental surveillance was un...
Article
Full-text available
The original version of this article unfortunately contained a mistake. The presentation of Table 1 was incorrect. The corrected table is given below. The original article has been corrected. © 2018 Springer Science+Business Media, LLC, part of Springer Nature
Article
Full-text available
Noroviruses (NoV) are a major cause of gastroenteritis worldwide. Recently, a novel variant of NoV GII.17 (GII.P17_GII.17 NoV), termed Kawasaki 2014, has been increasingly reported in NoV outbreaks in Asia, and has also been described in Europe and North America. In this study, sewage samples were investigated to study the occurrence and genetic di...
Article
Human adenoviruses (HAdVs) are of major public health importance and are associated with a variety of clinical manifestations, including gastroenteritis, respiratory, ocular and urinary tract infections.
Article
Full-text available
Full-genome sequences have been used to monitor the fine-scale dynamics of epidemics caused by RNA viruses. However, the ability of this approach to confidently reconstruct transmission trees is limited by the knowledge of the genetic diversity of viruses that exist within different epidemiological units. In order to address this question, this stu...
Article
Full-text available
Foot-and-mouth disease viruses are often restricted to specific geographical regions and spread to new areas may lead to significant epidemics. Phylogenetic analysis of sequences of the VP1 genome region of recent outbreak viruses from Libya and Saudi Arabia has revealed a lineage, O-Ind-2001, normally found in the Indian subcontinent. This paper d...
Article
The stamping-out policy for the control of foot-and-mouth disease virus (FMDV) in countries that are free from FMD without vaccination has a dramatic socio-economic impact, huge animal welfare issues and may result in the loss of farm animal genetic resources. As an alternative to pre-emptive culling or emergency vaccination we further explore the...
Article
Full-text available
Foot-and-mouth disease Virus (FMDV) is an economically important, highly contagious picornavirus that affects both wild and domesticated cloven hooved animals. In developing countries, the effective laboratory diagnosis of foot-and-mouth disease (FMD) is often hindered by inadequate sample preservation due to difficulties in the transportation and...
Article
Full-text available
Foot-and-mouth disease (FMD) is endemic in most countries of southern Africa where it affects cloven-hoofed animals that include livestock and wildlife. Southern Africa relies profoundly on livestock production as a source of economic growth and livelihoods. Despite the importance of FMD in southern Africa, the epidemiology of FMD virus (FMDV) and...
Article
Full-text available
The complete genomes of foot-and-mouth disease (FMD) viruses recovered in Libya and Saudi Arabia in 2013 are described here. These viruses belong to an FMD virus lineage (Ind-2001, topotype Middle East-South Asia, serotype O) which is normally endemic in the Indian subcontinent. A contemporary virus sequence from Bhutan is also reported here.
Article
Full-text available
Advances in sequencing technology coupled with new integrative approaches to data analysis provide a potentially transformative opportunity to use pathogen genome data to advance our understanding of transmission. However, to maximize the insights such genetic data can provide, we need to understand more about how the microevolution of pathogens is...
Data
Full-text available
The epidemiology of foot-and-mouth disease (FMD) in North Africa is complicated by the co-circulation of endemic FMD viruses (FMDV), as well as sporadic incursions of exotic viral strains from the Middle East and Sub-Saharan Africa. This report describes the molecular characterization of SAT 2 FMD viruses that have caused widespread field outbreaks...
Article
Foot-and-mouth disease (FMD) is a highly infectious viral disease of cloven-hoofed animals with debilitating and devastating consequences for livestock industries throughout the world. Key antigenic determinants of the causative agent, FMD virus (FMDV), reside within the surface-exposed proteins of the viral capsid. Therefore, characterization of t...
Data
Oligonucleotide primers used for the FMDV amplification and sequencing at The Pirbright Institute (UK). (DOCX)
Data
Evaluation of the temporal signal and ‘clock-likeness’ of the data (Path-O-Gen). A. Regression of root-to-tip distances against date of sampling of 19 sequences to investigate the ‘clock-likeness’ of its molecular phylogeny. B. ‘Best fitting root’ to the hypothesis that the 19 viruses have a constant rate of evolution. (TIF)
Data
Estimation of viral effective population size (Neτ, interpretable as the product of the effective number of infected animals and the virus generation time) through time using a Bayesian skyline plot (BEAST). Five potential population size transitions underlay the demographic model. The thick solid black line is the median estimate and the blue line...
Data
Oligonucleotide primers used for the FMDV amplification and sequencing at the National Veterinary Institute (Denmark). (DOCX)
Data
Spatiotemporal reconstruction of the incursion of FMDV into Bulgaria from Anatolia (Turkey). (MP4)
Data
Spatiotemporal reconstruction of the incursion of FMDV within Burgas region (Bulgaria). (MP4)
Article
Full-text available
Analysis of full genome sequences was previously used to trace the origin and transmission pathways of foot-and-mouth disease virus (FMDV) outbreaks in the United Kingdom in 2001 and 2007. Interpretation of these data was sometimes at variance with conventional epidemiological tracing, and was also used to predict the presence of undisclosed infect...
Article
Full-text available
The epidemiology of foot-and-mouth disease (FMD) in North Africa is complicated by the co-circulation of endemic FMD viruses (FMDV), as well as sporadic incursions of exotic viral strains from the Middle East and Sub-Saharan Africa. This report describes the molecular characterization of SAT 2 FMD viruses that have caused widespread field outbreaks...
Article
Full-text available
Two foot-and-mouth disease virus (FMDV) genome sequences have been determined for isolates collected from recent field outbreaks in North Africa (Egypt) and the Middle East (Palestinian Autonomous Territories). These data represent the first examples of complete genomic sequences for the FMDV SAT 2 topotype VII, which is thought to be endemic in co...
Article
Full-text available
Using foot-and-mouth disease (FMD) as an example, this review describes new tools that can be used to detect and characterise livestock diseases. In recent years, molecular tests that can detect and characterise pathogens in a diverse range of sample types have revolutionised laboratory diagnostics. In addition to use in centralised laboratories, t...
Article
Full-text available
Foot-and-mouth disease (FMD) outbreaks recently affected 2 countries (Japan and South Korea) in eastern Asia that were free of FMD without vaccination. Analysis of viral protein 1 nucleotide sequences indicated that FMD serotype A and O viruses that caused these outbreaks originated in mainland Southeast Asia to which these viruses are endemic.
Article
Full-text available
FURTHER to the letter from Nigel Gibbens, the UK Chief Veterinary Officer ( VR , February 5, 2011, vol 168, pp 136–137), we would like to provide additional information about the recent appearance of foot-and-mouth disease (FMD) in south-eastern Bulgaria. Since the end of December 2010, three outbreaks of FMD have been reported in Burgas region, c...
Presentation
Full-text available
This presentation outlines results from a 1 year collaborative project funded by FAO (EuFMD): Application of Tools for High-Resolution FMDV Molecular Epidemiology in Western EurAsia
Poster
Full-text available
Full-genome sequencing methods for FMDV provide molecular epidemiology data at greater resolution data than those generated by “conventional” methods such as VP1 analysis. This opens up the potential for using genome sequencing to reveal and identify the origin of un-clarified transmission events within epidemics. The aim of this study was to devel...
Article
The prevalence of border disease virus (BDV) viraemia in Spanish lambs was determined from 2089 sera randomly collected at two slaughterhouses in 2001 and 2003, as well as in 126 sera obtained in 2004 from a fattening unit with an acute disease problem. BDV was detected with an indirect peroxidase monolayer assay (IPMA), and for the fattening unit...
Article
Between 2001 and 2002, samples from 1,413 animals in 21 Spanish small ruminant flocks, most of them with animals showing clinical signs compatible with Border disease (BD), were screened for the presence of Pestivirus antigen and antibodies by an indirect peroxidase monolayer assay (IPMA) and the virus neutralization test (VNT), respectively. Altho...

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