B. B. Dzantiev

• Russian Academy of Sciences

The effects of magnesium and sodium on the interactions between aptamer, its specific ligand, and short complementary oligonucleotides (cDNAs) differing in affinity of their binding with the aptamer were studied. Aflatoxin B1 (AFB1) and AFB1-binding aptamer were used in the study. Dependencies for the aptamer binding with the fluorophore-labeled AF...

An Erratum to this paper has been published: https://doi.org/10.1134/S1061934825010058

Nanozymes are synthetic nanomaterials with enzyme-like catalytic activity. Since catalytic reactions occur on the surface of nanoparticles, changing their surface properties (charge, chemical composition, adsorbed molecules, etc.) is a tool for tuning the catalytic properties of nanozymes. The review provides a comparative analysis of the chemical...

To ensure the safety of foodstuffs, widespread non-laboratory monitoring for pathogenic contaminants is in demand. A suitable technique for this purpose is lateral flow immunoassay (LFIA) which combines simplicity, rapidity, and productivity with specific immune detection. This study considered three developed formats of LFIA for Salmonella Typhimu...

A membrane test system has been developed for the rapid determination of bisphenol A in drinking water, utilizing a conjugate of gold nanoparticles with an aptamer that specifically binds the target analyte, and a conjugate of mercaptosuccinic acid with a carrier protein impregnated in the test zone of the strip. The working principle of the test s...

Gatifloxacin (GAT), an antibiotic belonging to the fluoroquinolone (FQ) class, is a toxicant that may contaminate food products. In this study, a method of ultrasensitive immunochromatographic detection of GAT was developed for the first time. An indirect format of the lateral flow immunoassay (LFIA) was performed. GAT-specific monoclonal antibodie...

Pectobacterium brasiliense is a bacterial phytopathogen that causes soft and black rot and actively spreads worldwide. Our study is the first development of immunoassays for detecting P. brasiliense. We immunized rabbits and obtained serum with an extremely high titer (1:10⁸). Isolated polyclonal antibodies were tested by enzyme-linked immunosorben...

Nonylphenol is an aromatic organic compound that has an estrogen-like effect and has a negative effect on the human endocrine system. A method has been developed for the competitive determination of nonylphenol using magnetic particles, rabbit antiserum, nonylphenol conjugate with soybean trypsin inhibitor (STI) and biotin. The principle of the ana...

Uncontrolled use of pesticides in agriculture leads to negative consequences for the environment, as well as for human and animal health. Therefore, timely detection of pesticides will allow application of measures to eliminate the excess of maximum residue limits and reduce possible negative consequences in advance. Common methods of pesticide ana...

A bimetallic core–shell-shell nanorods structure with gap-embedded Raman reporter 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) was developed and applied as a SERS-active nanotag in surface-enhanced Raman scattering lateral flow immunoassay (SERS-LFIA) of the insecticide fipronil. Due to the strong SERS signal of the AuDTNB@AgDTNB@AgNRods, fipronil...

Background and Aim In animal husbandry, antibiotics are frequently used as growth promoters, as well as for illness prevention and treatment. They are considered important toxic and allergenic contaminants of food and a serious risk factor for the spread of antibiotic resistance. National and international regulatory authorities have established li...

When developing immunochemical test systems, it is necessary to obtain specific antibodies. Their quality depends, among other things, on the immunogen used. When preparing hapten–protein conjugates to obtain antibodies for low-molecular-weight compounds, the key factors are the structure of the hapten itself, the presence of a spacer, the size of...

Tylosin (TYL) is a macrolide antibiotic widely used in animal husbandry. Due to associated health risks, there is a demand for sensitive methods for mass screening of TYL in products of animal origin. This article describes the development of lateral flow immunoassays (LFIAs) for TYL detection using direct (anti-TYL antibodies conjugated with nanop...

A promising and sought-after class of nanozymes for various applications is Pt-containing nanozymes, primarily Au@Pt, due to their easy preparation and remarkable catalytic properties. This study aimed to explore the freeze–thaw method for functionalizing Pt-containing nanozymes with oligonucleotides featuring a polyadenine anchor. Spherical gold n...

The influence of organic solvents such as methanol and acetonitrile on the results of immunodetermination of butachlor in samples of rice and rice products was studied. The schemes of enzyme immunoassay using: (a) native antiserum containing specific antibodies to butachlor and antispecies antibodies labeled with horseradish peroxidase, and (b) bio...

Fluorescence induced by the excitation of a fluorophore with plane-polarized light has a different polarization depending on the size of the fluorophore-containing reagent and the rate of its rotation. Based on this effect, many analytical systems have been implemented in which an analyte contained in a sample and labeled with a fluorophore (usuall...

Adulteration of meat products is a serious problem in the modern society. Consumption of falsified meat products can be hazardous to health and/or lead to violating religious dietary principles. To identify such products, rapid and simple test systems for point-of-need detection are in demand along with complex laboratory methods. This study presen...

A competitive chemiluminescent immunoenzyme assay (CL-EIA) technique is proposed for the sensitive determination of one of the environmentally significant toxicants of anthropogenic origin-nonylphenol—in natural waters. The chosen chemiluminescent detection is characterized by a higher sensitivity compared to the colorimetric. The limit of nonylphe...

In the current economic situation, after easing the Covid pandemic restrictions, almost all laboratories, which are focused on evaluation of the conformity of food products, have faced issues in supplying for their laboratories. In this regard, in the last years many laboratories have been forced to validate new approaches and introduce new methods...

CRISPR/Cas12a is a potent biosensing tool known for its high specificity in DNA analysis. Cas12a recognizes the target DNA and acquires nuclease activity toward single-stranded DNA (ssDNA) probes. We present a straightforward and versatile approach to transforming common Cas12a-cleavable DNA probes into enhancing tools for fluorescence anisotropy (...

In this study, we developed a sensitive immunochromatographic analysis (ICA) of the Salmonella typhimurium bacterial pathogen contaminating food products and causing foodborne illness. The ICA of S. typhimurium was performed using Au@Pt nanozyme as a label ensuring both colorimetric detection and catalytic amplification of the analytical signal due...

The analytical applications of antibodies are often associated with their immobilization on different carriers, which is accompanied by a loss of antigen-binding activity for a sufficient proportion of the bound antibodies. In contrast to data on plain carriers, minimal data are available on the properties of antibodies on the surfaces of nanoparti...

This study was aimed at the sensitive immunodetection of porcine myoglobin (MG) as a species-specific biomarker in meat products. The enhanced lateral flow immunoassay (LFIA) was created in the sandwich format using monoclonal antibodies (Mab) with specificity to porcine MG and labeled by Prussian blue nanoparticles (PBNPs) as peroxidase-mimicking...

Colored silica particles with encapsulated bromophenol blue dye were synthesized and characterized as potential markers for lateral flow immunoanalytical systems. Initial concentrations of monomers (aminopropyltriethoxysilane and tetraetoxysilan) and time of their polymerization were varied, and the influence of these parameters on size and optical...

The toxic effects of antimony pose risks to human health. Therefore, simple analytical techniques for its widescale monitoring in water sources are in demand. In this study, a sensitive microplate apta-enzyme assay for Sb³⁺ detection was developed. The biotinylated aptamer A10 was hybridized with its complementary biotinylated oligonucleotide T10 a...

Lateral flow immunoassay (LFIA) has found a broad application for testing in point-of-care (POC) settings. LFIA is performed using test strips—fully integrated multimembrane assemblies containing all reagents for assay performance. Migration of liquid sample along the test strip initiates the formation of labeled immunocomplexes, which are detected...

CRISPR/Cas12-based biosensors are emerging tools for diagnostics. However, their application of heterogeneous formats needs the efficient detection of Cas12 activity. We investigated DNA probes attached to the microplate surface and cleaved by Cas12a. Single-stranded (ss) DNA probes (19 variants) and combined probes with double-stranded (ds) and ss...

Reliable detection of specific antibodies against pathogens by lateral flow immunoassay (LFIA) greatly depends on the composition of the detectable complex and the order of its assembly. We compared three LFIA formats for revealing anti-SARS-CoV-2 antibodies in sera with the following detected complexes in the analytical zone of the strip: antigen–...

The use of plasticizers to improve the quality of plastics widely used for household purposes inevitably leads to an increase in their pollution of food and environmental objects. Diisobutyl phthalate (DiBP) is one of the ortho-substituted phthalic acid esters that negatively affect human health and ecosystems. This work is directed to the developm...

Biosensors based on endonuclease Cas12 provide high specificity in pathogen detection. Sensitive detection using Cas12-based assays can be achieved using trans-cleaved DNA probes attached to simply separated carriers, such as magnetic particles (MPs). The aim of this work was to compare polyA, polyC, and polyT single-stranded (ss) DNA with differen...

In this study, an ultrasensitive immunochromatographic determination of the muscle tissue of pork was developed. Myoglobin (MG), a protein contained in skeletal muscles, was proposed as a molecular identifier of pork via the application of specific monoclonal antibodies (MAb) recognizing the porcine form of this protein. The immunochromatographic a...

Pathogen identification requires nucleic acid diagnosis with simple equipment and fast manipulation. Our work established an all-in-one strategy assay with excellent sensitivity and high specificity, Transcription-Amplified Cas14a1-Activated Signal Biosensor (TACAS), for the fluorescence-based bacterial RNA detection. The DNA as a promoter probe an...

Phthalic acid esters are integral components of modern plastic products and packaging materials, which causes significant contamination of food products and the environment, leading to the need for simple productive monitoring methods. This article presents a rapid enzyme-linked immunosorbent assay (ELISA) for the determination of dibutyl phthalate...

Finding optimal conditions for competitive lateral flow immunoassay is a controversial task. The content of specific antibodies labeled by nanoparticles should be simultaneously high to reach intense signals and low to register an influence on the signals for minimal concentrations of the target analyte. We propose to use two kinds of complexes of...

Phthalic acid esters are integral components of modern plastic products and packaging materials, which causes significant contamination of food products and the environment, leading to the need for simple productive monitoring methods. The article presents a rapid enzyme-linked immunosorbent assay (ELISA) for the determination of dibutyl phthalate...

A scheme of modular competitive immunochromatography with an analyte-independent test strip and changeable specific immunoreactants has been proposed. Native (detected) and biotinylated antigens interact with specific antibodies during their preincubation in solution, that is, without the immobilization of reagents. After this, the detectable compl...

Antibiotic usage has become very widespread in aquaculture, and the abuse or overuse of antibiotics has led to the evolution of antibiotic-resistance bacteria, which has adverse effects on aquatic products and ecosystems. Moreover, this evolution can potentially cause harm to human health. Thus, there is an urgent need for diagnostic tools for anti...

Sequence-specific endonuclease Cas12-based biosensors have rapidly evolved as a strong tool to detect nucleic acids. Magnetic particles (MPs) with attached DNA structures could be used as a universal platform to manipulate the DNA-cleavage activity of Cas12. Here, we propose nanostructures of trans- and cis-DNA targets immobilized on the MPs. The m...

Surface-enhanced Raman scattering (SERS) is considered an efficient technique providing high sensitivity and fingerprint specificity for the detection of pesticide residues. Recent developments in SERS-based detection aim to create flexible plasmonic substrates that meet the requirements for non-destructive analysis of contaminants on curved surfac...

Changes in the limits of detection (LODs) for a multiplex lateral flow immunoassay (LFIA) caused by different locations of the binding zone on the test strips were studied. Due to the non-equilibrium conditions of the immune reactions in LFIAs, their analytical parameters are susceptible to the binding constants of antigen–antibody reactions and as...

The work is dedicated to the use of complexes of aptamers with complementary oligonucleotides. In the presence of target the oligonucleotide is displaced from the complex, which is accompanied by a change in the registered fluorescent properties of the label. The obtained regularities were applied for the detection of mycotoxins: ochratoxin A (dete...

Fluoroquinolone antibiotics are used to cure and protect bees and apiaries from infections. Consequently, they may contaminate honey and other products of beekeeping. In this study, a highly sensitive immunoenzyme...

Gold spherical nanoparticles, gold-platinum nanoflowers, and gold-silver nanostars were obtained and compared as labels for immunochromatographic analysis. The nanoparticles were synthesized by chemical reduction from various precursors and then conjugated with staphylococcal protein A to be used in indirect immunochromatographic determination of n...

Isothermal amplifications allow for the highly sensitive detection of nucleic acids, bypassing the use of instrumental thermal cycling. This work aimed to carry out an experimental comparison of the four most promising techniques: recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) coupled with lateral flow...

The work considers a combination of three enhancing approaches for immunochromatographic assay (ICA) and the integration of their impacts into changes of the limit of detection (LOD). Human fatty acid binding protein (FABP), an early biomarker of acute myocardial infarction, was the target analyte. Starting from the common ICA protocol with an LOD...

Assessment of the composition of meat-containing products is the task in demand due to their frequent deviations from declared recipes. The paper presents the developed test system for immunochromatographic determination of total meat content. The assay is based on the simultaneous use of monoclonal antibodies, which specifically interacts with mam...

The paper presents development and characterization of a new bioanalytical test system for rapid detection of lipopolysaccharide (LPS) and whole cells of Francisella tularensis, a causative agent of tularemia, in water samples. Gold nanoparticles (AuNPs) coated by the obtained anti-LPS monoclonal antibodies were used for the assay. Their contact wi...

In this study, a lateral flow immunoassay (LFIA) was developed to detect okadaic acid (OA) belonging to the diarrheic shellfish poisoning group of aquatic toxins. Newly obtained anti-OA monoclonal antibodies and bimetallic core@shell Au@Pt nanoparticles were used in the indirect format of the LFIA. Peroxidase-mimicking nanozyme properties of Au@Pt...

The use of a large amount of toxic synthetic materials leads to an increase in the pollution of environmental objects. Phthalates are compounds structurally related to esters of phthalic acid that are widely used in the manufacturing of synthetic packaging materials as plasticizers. Their danger is conditioned by leaching into the environment and p...

The aim of this study was to develop a novel immunochromatographic analysis (ICA) for the determination of lamb additives in raw meat and meat products. Sheep immunoglobulins of class G (IgG) served as the molecular identifier. The ICA was implemented in a sandwich format using anti-species antibodies labeled with gold nanoparticles. Sheep IgG was...

Mercury pollution is a global environmental problem, especially in low-resource areas where artisanal iron mining is taking place and industrialization is on the rise. Therefore, there is a demand for simple methods for the determination of toxic metals at low. In this study, an on-field membrane lateral flow test system for sensitive and specific...

Aquatic toxins are a group of toxic compounds produced by several types of freshwater and marine algae and cyanobacteria and transported through the food chains of water bodies. Potential contamination of aquaculture products (raw and processed fish and seafood) with aquatic toxins requires the use of efficient screening methods for their control....

In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under optimal conditions, the instrumental detection li...

The detection of ochratoxin A (OTA), a harmful toxic contaminant of foods causing serious health problems, is of great importance. Common analytical methods for this purpose are expensive, time- and labor-consuming. To overcome these limitations, a fluorescence resonance energy transfer (FRET)-based immunosensor for the detection of ochratoxin A (O...

Hydrochlorothiazide, an antihypertensive and diuretic drug only for clinic purpose, was recently found as an adulterant in functional foods. Due to the potential health risk, it is urgent to develop a simple and rapid assay for its identification. In this work, two hapten structures were rationally designed based on molecular modeling, and a highly...

An immunochromatographic test system based on the indirect competitive format has been developed for the rapid control of cyanotoxin microcystin-LR (MC-LR) in drinking water. To increase the assay sensitivity, a hydroquinone-based reduction of silver ions on the surface of gold nanoparticles as part of the immune complexes formed on the immunochrom...

The presence of pathogen-specific antibodies in the blood is widely controlled by a serodiagnostic technique based on the lateral flow immunoassay (LFIA). However, its common one-stage format with an antigen immobilized in the binding zone of a test strip and a nanodispersed label conjugated with immunoglobulin-binding proteins is associated with r...

A lateral flow immunoassay (LFIA) of phycotoxin domoic acid (DA) contaminating seawater and marine organisms was developed in this investigation. Nine clones of monoclonal antibodies against DA were produced and characterized. The test system was implemented in the indirect competitive format, where gold nanoparticles as a marker were conjugated wi...

Short oligonucleotides are widely used for the construction of aptamer-based sensors and logical bioelements to modulate aptamer–ligand binding. However, relationships between the parameters (length, location of the complementary region) of oligonucleotides and their influence on aptamer–ligand interactions remain unclear. Here, we addressed this t...

Nowadays, the presence of pathogen-specific antibodies in the blood is widely controlled by a serodiagnostic technique based on the lateral flow immunoassay (LFIA). However, its common one-stage format with an antigen immobilized in the binding zone of a test strip and a nanodis-persed label conjugated with immunoglobulin-binding proteins is associ...

A new bioanalytical labeling system based on alloyed quantum dots' (QDs) photoluminescence quenching caused by an enzymatic reaction has been developed and tested for the first time. The catalytic role of the enzyme provides high sensitivity and the possibility of varying detecting time to improve assay sensitivity. Alloyed luminescent QDs were cho...

CRISPR-Cas12-based biosensors are a promising tool for the detection of nucleic acids. After dsDNA-target-activated Cas12 cleaves the ssDNA probe, a lateral flow test (LFT) is applied for rapid, simple, and out-of-laboratory detection of the cleaved probe. However, most of the existing approaches of LFT detection have disadvantages related to inver...

The growing interest in the development of new platforms for the application of Raman spectroscopy techniques in biosensor technologies is driven by the potential of these techniques in identifying chemical compounds, as well as structural and functional features of biomolecules. The effect of Raman scattering is a result of inelastic light scatter...

The current COVID-19 pandemic has increased the demand for pathogen detection methods that combine low detection limits with rapid results. Despite the significant progress in methods and devices for nucleic acid amplification, immunochemical methods are still preferred for mass testing without specialized laboratories and highly qualified personne...

The detection limit of lateral flow immunoassay (LFIA) is largely determined by the properties of the label used. We compared four nanoparticle labels differing in their chemical composition and colour: (1) gold nanoparticles (Au NPs), red; (2) Au-core/Pt-shell nanoparticles (Au@Pt NPs), black; (3) latex nanoparticles (LPs), green; and (4) magnetic...

This study presents the development of a qualitative double immunochromatographic analysis (ICA) for the rapid simultaneous determination of ovalbumin (OVA) and immunoglobulins of class Y (IgY) of chicken origin. The latter serve as biomarkers to control the presence of chicken meat and eggs in food products. Polyclonal antibodies against OVA were...

In this study, tannic acid-modified gold nanoparticles were found to have superior nanozyme activity and catalyze the oxidation reaction of 3,3′,5,5′-tetramethylbenzidine in the presence of hydrogen peroxide. Enhancing the catalytic activity of the nanozyme by Pb²⁺ ions caused by selectively binding metal ions by the tannic acid-capped surface of g...

Heavy metal pollution is still of actual task despite on many developments. The growth in nanotechnology allowed for application of nanoparticles modified with receptor molecules for selective or group-selective recognition of concrete metal ions. One of these molecules are aptamers which consist of nucleotides linked in necessary order. In our inv...

A device for multiplex lateral flow immunoassay (LFIA) of major viral (potato viruses X and Y, potato leafroll virus) and bacterial (Ralstonia solanacearum, Clavibacter michiganensis, Dickeya solani, Pectobacterium atrosepticum) potato pathogens was developed. The device includes test strips connected with the central fiberglass reservoir. The addi...

Antibody–nanoparticle conjugates are widely used analytical reagents. An informative parameter reflecting the conjugates’ properties is the number of antibodies per nanoparticle that retain their antigen-binding ability. Estimation of this parameter is characterized by a lack of simple, reproducible methods. The proposed method is based on the regi...

Verifying the authenticity of food products is essential due to the recent increase in counterfeit meat-containing food products. The existing methods of detection have a number of disadvantages. Therefore, simple, cheap, and sensitive methods for detecting various types of meat are required. In this study, we propose a rapid full-cycle technique t...

Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens. Commonly, PCR techniques are used in laboratories for highly sensitive detection of DNA/RNA from viral, viroid, bacterial, and fungal pathogens of plants. However, using PCR-based methods for in-field diagnostics is a challenge and sometimes nearly impossib...

The combination of recombinase polymerase amplification (RPA) and lateral flow test (LFT) is a strong diagnostic tool for rapid pathogen detection in resource-limited conditions. Here, we compared two methods generating labeled RPA amplicons following their detection by LFT: (1) the basic one with primers modified with different tags at the termina...

Many studies have found that gold nanoparticles with branched surfaces (nanoflowers) are markers for immunosensors that provide higher sensitivity gains than the commonly used spherical gold nanoparticles. Although the analytical characteristics of nanoparticle-using systems vary significantly depending on their size and shape, the question of choo...

The development of reliable and highly sensitive methods for heavy metal detection is a critical task for protecting the environment and human health. In this study, a qualitative colorimetric sensor that used mercaptosuccinic-acid-functionalized gold nanoparticles (MSA-AuNPs) to detect trace amounts of Fe(III) ions was developed. MSA-AuNPs were pr...

Potato blackleg caused by Dickeya spp. bacteria is one of the most important bacterial diseases of potatoes. The rapid spread of this disease in the territory of Russia requires new effective diagnostic tools for the timely detection of infection. To solve this problem, antisera specific to Dickeya spp. were obtained. Polyclonal antibodies isolated...

In this study, we developed a lateral flow immunoassay (LFIA) for the detection of pork additives in meat products. LFIA of porcine immunoglobulins (IgG) as a molecular biomarker was carried out in a sandwich format for species identification. Gold nanoparticles as a nano-dispersed label were conjugated to secondary antibodies specific to anti-porc...

In this study, an immunochromatographic test system for the ultrasensitive and rapid detection of the highly toxic phycotoxin microcystin-leucine-arginine (MC-LR) has been developed. The lateral flow immunoassay (LFIA) is based on an indirect competitive format with the use of magnetic particles (MPs) as a colored label for secondary antibodies and...