B. Babu

• M.Pharm. PhD
• Faculty Member at JSS College of Pharmacy (ooty)

An effective, simple and sensitive analytical method has been developed employing liquid chromatography coupled with tandem mass spectrometry and validated for estimation of 5 carbamate pesticides at trace levels in 3 fruits and 4 vegetables. Plackett-Burman design and central composite design was used to screen and optimize the significant factors...

Article history Pesticide residues in food and water have been linked to birth defects, foetal toxicity, cancers, genetic defects, blood disorders, neurotoxicity and endocrine disruption. It is obvious that analysis of pesticide is important not only in the development process but also in providing the detailed information about the food stuffs tha...

This study implies to development of a sensitive liquid chromatography tandem mass spectrometry method. Further using this method ribociclib can be estimated in the drug formulation. Ribociclib was recently approved by the United States Food and Drug Administration in the year 2017 for metastatic and advanced breast cancer, which is used for resist...

Cyproterone acetate (CA) is an anti-androgenic drug with progestogenic activity. CA binds to the androgen receptor and prevents androgen-induced receptor activation in target tissues, inhibiting the growth of testosterone-sensitive tumor cells. The current method aims to develop an RP-HPLC method for the detection of CA in both its pure and formula...

A new simple, accurate and precise HPLC method has been developed and validated for estimation of Azelnidipine in its formulation. In RP-HPLC method, a C8 column and methanol: water (85:15), pH adjusted to 3.0 using orthophosphoric acid were used at a flow rate of 1.0 mL/min and detected at 257 nm. The retention time for Azelnidipine was found to b...

Zonisamide (ZON) is an anti epileptic used for adjunctive therapy of partial seizures in adults, and children (6 years and above). Many methods for the estimation of Zonisamide have been reported which include spectrophotometry and HPLC methods. A validated HPLC method has been developed for estimation of Zonisamide in its formulation. In HPLC meth...

The objective of present work was to develop and validate forced degradation analysis and characterization of degradation products of sixteen membered lactone ring macrolide antibiotic Spiramycin under hydrolytic, oxidative, thermal and photolytic stress degradation condition as per the International Conference on Harmonization (ICH) guidelines. A...

A bioanalytical method for the quantification of rosiglitazone on rat plasma and tissues (adipose tissue, heart, brain, bone, and kidney) using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was developed and validated. Chromatographic separation was achieved on Gemini C18 column (50mm x 4.6mm, 3μm) using mobile phase consis...

A novel and reliable stability-indicating high-performance liquid chromatography method was developed using design of experiments. Under forced degradation conditions (hydrolysis, oxidative, photolytic and thermal) Nilotinib produced five major degradation products utilizing sodium hydroxide in base hydrolysis. The degradation products were separat...

A simple, sensitive and rapid high performance liquid chromatographic method has been developed for the determination of Propofol. The main focus of the method was to determine Propofol in solution form as well as in marketed formulation. Chromatographic separation was achieved on Inertsil ODS-3V column (250mm x 4.6mm; 5µm) with a mobile phase cons...

Background Several analytical approaches for assessing anti-cancer drugs in pharmaceutical formulations have been developed over the last few years. QbD plays an important in establishing accurate analytical methods to analyze anti-cancer compounds. Quality by design has become a risk analysis as well as a science-based strategy, implemented by exp...

In the present study, a sensitive LC-MS/MS method was developed and validated to measure pioglitazone (PGZ) concentrations in rat plasma and tissues. The chromatographic separation was achieved by using a YMC Pro C18 column (100 mm × 4.6 mm, 3μ) with a mobile phase consisting of formic acid (0.1% v/v) and acetonitrile (5 : 95) at a flow rate of 0.7...

The present study aimed to investigate the dissipation kinetics and residual levels of five organophosphate pesticides in a carrot- and potato-field ecosystem by sensitive and selective liquid chromatography-tandem mass spectrometry method. The optimization of the modified QuEChERS extraction method was carried out using the cost-effective chemomet...

The present study was aimed to develop and validate a simple, sensitive and economical bio-analytical high-performance liquid chromatographicultraviolet method for the determination of irbesartan in human plasma. The method involves the use of simple precipitation method for the determination of irbesartan, using methanol as precipitating agent and...

A novel simple, precise, accurate and a validated, liquid chromatography-tandem mass spectroscopy method has been developed for the quantitative determination of Midodrine hydrochloride from the commercially available formulation. Caffeine was used as the internal standard. Isocratic separation was achieved using jones C18 column (4.6 x 150mm, 3µm)...

Considering, the use of Fingolimod hydrochloride for the treatment of multiple sclerosis and its prophylaxis. The objective of the current study is to establish a simple, precise and rapid liquid chromatography tandem mass spectrometer method using electrospray ionization for the quantitative determination of the immunosuppressive drug Fingolimod h...

A wide variety of pesticides have been used in agriculture to increase the yield, quality and extend the storage life of crops. However, the use of pesticide has been increased now a day due to the ever-increasing population and rapid urbanization. The continuous uses of these pesticides have resulted in contamination of the environment, crops and...

Topiramate is an anticonvulsant used to treat seizures and prevent migraines. The aim of this study was to develop and validate a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify Topiramate in its formulation. Acetonitrile and ammonium acetate were used as a mobile phase (85:15 v/v ratio), and isocra...

A simple and sensitive liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) method has been developed and validated for the determination of Fingolimod in human blood. The analyte and internal standard (IS) Fingolimod‐d4 were extracted from 300 μL of human blood using protein precipitation coupled with solid‐phase extraction method. The chroma...

A stability-indicating high-performance liquid chromatography method for Pemetrexed bulk was developed and validated as per the ICH guidelines by employing design of experiments methodology equipped with Box-Behnken design. The separation of Pemetrexed and its oxidative degradation product was achieved in isocratic elution mode with Zorbax C18 colu...

An ultrafast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method was developed for the simultaneous estimation of artesunate (ART), dihydroartemisinin (DHA, an active metabolite of ART) and quercetin (QRT) in rat plasma. The separation was achieved using a Zorbax C18 column (3 μm, 50 mm × 4.6 mm) as a stationary phase with a mobile p...

Abstract A simple, sensitive, and specific liquid chromatography-tandem mass spectrophotometry (LC-MS/MS) method was developed and validated for the quantification of lenalidomide in human plasma. The separation was carried out on a symmetry, C18, 5-μm (50 × 4.6 mm) column as stationary phase and with an isocratic mobile phase of 0.1% formic acid i...

A simple, sensitive and rapid high performance liquid chromatographic method has been developed for the determination of Gemcitabine. Chromatographic separation was achieved on anInertsil ODS-3V column (250mm x 4.6mm; 5µm) with a mobile phase consisting of water: acetonitrile (90:10; pH 3.5 adjusted with orthophosphoric acid) with a flow rate of 1....

A simple, sensitive chiral liquid chromatographic method was developed for the separation and quantification of enantiomers Midodrine. A chiral PAK IG-3 (150 x 4.6 mm) 3µm column was used for the separation of the enantiomers. The mobile phase consists of 10 mM ammonium bicarbonate in water and acetonitrile in the ratio of 95:5, v/v with a flow rat...

An effective, simple and sensitive analytical method has been developed employing liquid chromatography coupled with tandem mass spectrometry and validated for estimation of five organophosphate pesticides at trace levels in six fruits and twelve vegetables. Plackett-Burman design and central composite design was used to screen and optimize the sig...

The present study reports on a liquid chromatography-tandem mass spectroscopy method for the analysis of Cilostazol drug substance in its formulation. The materials utilize a Zorbax SB C18 analytical column with electrospray ionization and a triple quadrupole mass detector in multiple reaction monitoring (MRM) mode, resulting in a short analysis of...

The current study reports on a mass spectrometric method for the quantification of gemfibrozil drug substance in its formulation. Isocratic method was used for separation with help of Zorbax SB C 18 column (4.6 × 50 mm, 3 µm) using a triple quadrupole mass detector in multiple reaction monitoring (MRM) mode and electrospray ionization as ionization...

A validated direct chiral HPLC method was developed for the separation of enantiomers of Mefloquine. The separation was achieved using chiral PAK IG-3 (250 x 4.6 mm) 3μm column. The mobile phase was composed of 10 mM ammonium acetate and methanol in the ratio of 30:70, v/v. The flow rate of the mobile phase was set at 0.7 ml/min. The detection wave...

A rapid LC-MS/MS method has been developed and validated for the quantitative determination of Febuxostat from the commercially available formulations. The separation was achieved using Zorbax SB C18 column (4.6 × 50 mm, 5 μm) as a stationary phase and the mobile phase consists of (10 mM) Ammonium formate (pH4.0): methanol (10:90 v/v) with a flow o...

Objective: In the present study, an isocratic chiral reverse-phase high-performance liquid chromatography method was developed and the resolution of the drug and complete separation from its degradation products were successfully achieved. Methods: An isocratic method developed with a Phenomenex Lux 5 μ Cellulose 1 (150 mm×4.6 mm i.d., 5 μ) using U...

Objective: The present study was aimed to develop a rapid, specific and sensitive method based on high performance liquid chromatographic method was developed for the determination of telmisartan using indapamide as an internal standard.Methods: The utilization of single step protein precipitation method using methanol as a precipitating agent beco...

Objective: The purpose of this study was to develop a simple, sensitive, precise, and rapid LC-MS/MS method for quantitative determination of anti-hypertensive drug Riociguat in its formulation using electrospray ionization in positive mode. Method: In this method, a zorbax (50mm x 4.6mm x 5µm) column with mobile phase 0.1% Formic acid: Acetonitril...

Background Pantoprazole (PTZ) and Levosulpiride (LS) were proven as effective agents for the treatment of Gastro-Esophageal Reflux Disease (GERD). It is a complex motor disorder that results in regurgitation of the gastric contents into the lower esophagus with consequent symptoms such as heart burn, retrosternal pain, dysphagia and belching. Meth...

Objective The aim of the present work is to achieve a novel highly sensitive chromatographic method for the simultaneous determination of hepatitis C agents, sofosbuvir and velpatasvir from human plasma using ritonavir as an internal standard. Methods Chromatographic separation was achieved using Hypersil C18 column (50mm x 4.6mm, 3μm) with an iso...

A sensitive LC-MS/MS method has been developed and validated for the quantitative determination of Dexlansoprazole from the commercially available formulations. Omeprazole was used as the internal standard. Isocratic separation was achieved using Zorbax SB C 18 column (4.6 × 100 mm, 3 µm) as a stationary phase and the mobile phase consists of (0.5...

Background Midodrine (MD) is a prodrug which is converted into Desglymidodrine (DMD) after oral administration. Objective: The aim of the present study is to develop and validate a precise, accurate liquid chromatography- tandem mass spectroscopy (LC-MS/MS) method for the separation and detection of Midodrine and Desglymidodrine. Methods The qua...

Dasatinib is a medication used primarily to treat chronic myelogenous leukemia and selected cases of acute lymphoblastic leukemia. In the present study a validated stability-indicating LC-MS/MS method was developed for the estimation of Dasatinib and its degradants on Hypersil ODS (150mm × 4.6 mm i.d., 5μ) column using Methanol: 10mM Ammonium Forma...

Determination of Erlotinib hydrochloride in the presence of its degradation products was studied and validated by a RP-HPLC method. The RP-HPLC method was developed for the chromatographic separation of Erlotinib and its impurities by using Hibar C 18 (250x4.6 mm, 5 µm) column with a mobile phase combination of 10 mM ammonium formate with pH-4.0 an...

Soluble epoxide hydrolase (sEH) inhibition is reported to elevate endogenous epoxyeicosatrienoic acids (EET's), which are known to play an important role in neuroprotection by inhibiting oxidative stress and neuroinflammation. In the present study, PTUPB, a dual inhibitor of sEH and COX-2, has been tested for its antiparkinson activity against rote...

Objective: A stability indicating chiral high performance liquid chromatographic (HPLC) method was developed and validated for the separated (S) and (R) pioglitazone in raw material and its determination in the presence of degradation products formed during forced degradation studies. Methods: In the present study, an isocratic normal phase-HPLC me...

A novel simple, sensitive, and rapid isocratic reverse phase high-performance liquid chromatographic (RP-HPLC) method was developed for the quantitative determination of alosetron HCl, a 5-HT3 antagonist used in the treatment of severe irritable bowel syndrome (IBS) in females. The optimized chromatographic separation was achieved using a stationar...

A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the simultaneous estimation of ondansetron and ranitidine from pharmaceutical dosage forms. The method was carried out using a Phenomenex column C 18 (250 mm × 4.6 mm, i.d 5 μ) with a mobile phase consisting of 50 mM potassium dihydrogen orthophospha...

The aim of our present study is to develop oral sustained release dosage form for Losartan Potassium (SS) with wet granulation technique. Xanthan gum with additives MCC PH101 was used for the present study. The dissolution study is carried out. The studies indicated that the drug release can be modulated by varying the concentration of the polymer...

This work aims at investigating the use of xanthan gum to formulate sustained release matrix tablets containing rosiglitazone. The immediate release tablets and sustained release (SR) tablets of rosiglitazone were prepared by wet granulation and direct compression method respectively. Xanthan gum based tablet formulations alone showed high release...

A sensitive and reproducible high performance liquid chromatography (HPLC) method has been developed and validated for the quantification of metolazone in human plasma, after solid phase extraction (SPE). A Good resolution was achieved on a reverse-phase LichroCART Purospher® C₁₈ column using the mobile phase acetonitrile–0.5% triethylam...