Ayesha Saleem

Ayesha Saleem
University of Manitoba | UMN · Faculty of Kinesiology and Recreation Management

BSc (Hon), MSc, PhD

About

52
Publications
8,247
Reads
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1,910
Citations
Citations since 2016
24 Research Items
1327 Citations
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
Additional affiliations
September 2007 - August 2013
York University
Position
  • PhD, MSc

Publications

Publications (52)
Article
Full-text available
Endurance exercise-mediated multisystemic adaptations are known to mitigate metabolism-related disorders such as obesity and type 2 diabetes mellitus (T2DM). However, the underlying molecular mechanisms that promote crosstalk between organs and orchestrate the pro-metabolic effects of endurance exercise remain unclear. Exercise-induced release of p...
Article
Full-text available
Background: Human genetic disorders and transgenic mouse models have shown that mitochondrial DNA (mtDNA) mutations and telomere dysfunction instigate the aging process. Epidemiologically, exercise is associated with greater life expectancy and reduced risk of chronic diseases. While the beneficial effects of exercise are well established, the mol...
Article
Several studies have illustrated that the polymerase gamma mutator (PolG) mice have reduced mitochondrial content secondary to systemic mitochondrial dysfunction, and subsequently a lower capacity to perform aerobic respiration and endurance exercise. We sought to delineate the extent of glycolysis as a means of energy production in the PolG mice i...
Article
The major tumour suppressor protein p53 plays an important role in maintaining mitochondrial content and function in skeletal muscle. p53 has been shown to reside in the mitochondria complexed with mitochondrial DNA (mtDNA); however, the physiological repercussions of mitochondrial p53 remain unknown. We endeavoured to elucidate whether an acute bo...
Article
Full-text available
The purpose of this study was to evaluate the role of sirtuin 1 (SirT1) in exercise- and resveratrol (RSV)-induced skeletal muscle mitochondrial biogenesis. Using muscle-specific SirT1-deficient (KO) mice and a cell culture model of differentiated myotubes, we compared the treatment of resveratrol, an activator of SirT1, with that of exercise in in...
Article
Full-text available
Over the past several years, I have been interacting with an increasing number of Iranian scientists, including those currently living in Iran as well as others who are being educated elsewhere or have independent positions outside of that country. In all circumstances, the resulting collaborations have extended my own knowledge and allowed me to c...
Article
Full-text available
Exosomal release pathway and autophagy together maintain homeostasis and survival of cells under stressful conditions. Autophagy is a catabolic process through which cell entities, such as malformed biomacromolecules and damaged organelles, are degraded and recycled via the lysosomal-dependent pathway. Exosomes, a sub-type of extracellular vesicles...
Article
Full-text available
Background: Exercise is associated with health benefits, including the prevention and management of obesity. However, heterogeneity in the adaptive response to exercise training exists. Our objective was to evaluate if changes in extracellular vesicles (EVs) after acute aerobic exercise were associated with the responder phenotype following 6-week...
Article
Full-text available
Extracellular vesicles (EVs), released from all cells, are essential to cellular communication and contain biomolecular cargo that can affect recipient cell function. Studies on the effects of contractile activity (exercise) on EVs usually rely on plasma/serum-based assessments, which contain EVs from many different cells. To specifically character...
Preprint
Full-text available
Extracellular vesicles (EVs) are small lipid membrane-bound structures that are secreted by all cells, and play a central role in cellular communication. EVs are released from skeletal muscle during exercise, but the effects of contractile activity on skeletal muscle-derived EVs (Skm-EVs) are poorly understood due to the challenges in distinguishin...
Preprint
Full-text available
Extracellular vesicles (EVs) released from all cells, are essential to cellular communication, and contain biomolecular cargo that can affect recipient cell function. Studies on the effects of contractile activity (exercise) on EVs usually rely on plasma/serum-based assessments, which contain EVs from many different cells. To specifically character...
Article
Full-text available
Systemic hypoxia is a common element in most perinatal emergencies and is a known driver of Bnip3 expression in the neonatal heart. Bnip3 plays a prominent role in the evolution of necrotic cell death, disrupting ER calcium homeostasis and initiating mitochondrial permeability transition (MPT). Emerging evidence suggests a cardioprotective role for...
Article
Full-text available
Asthma is the most common pediatric disease, characterized by chronic airway inflammation and airway hyperresponsiveness. There are several management options for asthma, but no specific treatment. Extracellular vesicles (EVs) are powerful cellular mediators of endocrine, autocrine and paracrine signalling, and can modulate biophysiological functio...
Preprint
Full-text available
Exercise is associated with various health benefits, including the prevention and management of obesity and cardiometabolic risk factors. However, a strong heterogeneity in the adaptive response to exercise training exists. The objective of this study was to evaluate if changes in extracellular vesicles (EVs) after acute aerobic exercise (AE) were...
Preprint
Full-text available
Systemic hypoxia is a common element in most perinatal emergencies and is a known driver of Bnip3 expression in the neonatal heart. Bnip3 plays a prominent role in the evolution of necrotic cell death, disrupting ER calcium homeostasis and initiating mitochondrial permeability transition (MPT). Emerging evidence suggests a cardioprotective role for...
Article
Full-text available
Extracellular vesicles (EVs) are membranous vesicles secreted by both prokaryotic and eukaryotic cells and play a vital role in intercellular communication. EVs are classified into several subtypes based on their origin, physical characteristics, and biomolecular makeup. Exosomes, a subtype of EVs, are released by the fusion of multivesicular bodie...
Preprint
Extracellular vesicles (EVs) are membranous vesicles secreted by both prokaryotic and eukaryotic cells and play a vital role in intercellular communication. EVs are classified into several subtypes based on their origin, physical characteristics, and biomolecular makeup. Exosomes, a subtype of EVs, are released by the fusion of multivesicular bodie...
Preprint
Extracellular vesicles (EVs) are membranous vesicles secreted by both prokaryotic and eukaryotic cells and play a vital role in intercellular communication. EVs are classified into several subtypes based on their origin, physical characteristics, and biomolecular makeup. Exosomes, a subtype of EVs, are released by the fusion of multivesicular bodie...
Preprint
Full-text available
Asthma is the most common chronic disease in children. It is characterized by difficulty in breathing and chronic airway inflammation associated with narrowing of the airways, and airway hyperresponsiveness. If left untreated, asthma can lead to respiratory distress and even death. A number of medications are available and prescribed to manage asth...
Chapter
Obesity is a progressive chronic disease that is defined by increased adiposity and dysregulated blood lipid and glucose profiles. This, coupled with insulin resistance and hypertension, leads to the development of the metabolic syndrome in obese patients. Furthermore, obesity correlates strongly with an elevated risk and progression of a number of...
Preprint
Full-text available
A central focus of systems biology is the functional mapping of protein-protein interactions under physiological conditions. Here we describe MaGiCaL-BiFC, a lentivirus-based bimolecular fluorescence protein-fragment complementation approach for the high-throughput, genome-scale identification of protein-protein interactions in mammalian cells. Aft...
Chapter
Intriguingly, endurance exercise, obviously a highly beneficial activity in mitigating various metabolic diseases, is intimately related to a transient production of reactive oxygen species (ROS). The natural purpose of the ROS-initiated repair appears to be merely the cleanup of the damage caused by the acute ROS pulse, whether it was caused by ex...
Article
Recent research has suggested that transmembrane protein 65 (TMEM65) is localized within the inner mitochondrial membrane. Little else is known about its function. In this study we investigated the location and function of TMEM65. Further, we report the functional consequences of a novel homozygous splice variant (c.472+1G>A) in the TMEM65 gene in...
Article
Full-text available
The mtDNA mutator mouse lacks the proofreading capacity of the sole mtDNA polymerase, leading to accumulation of somatic mtDNA mutations, and a profound premature aging phenotype including elevated oxidative stress and apoptosis, and reduced mitochondrial function. We have previously reported that endurance exercise alleviates the aging phenotype i...
Article
Mitochondrial DNA (mtDNA) mutator mice express a mutated form of mtDNA polymerase gamma that results an accelerated accumulation of somatic mtDNA mutations in association with a premature aging phenotype. An exploratory metabolomic analysis of cortical metabolites in sedentary and exercised mtDNA mutator mice and wild-type littermate controls at 9-...
Article
Full-text available
The ATP demand required for muscle development is accommodated by elevations in mitochondrial biogenesis, through the co-ordinated activities of the nuclear and mitochondrial genomes. The most important transcriptional activator of the mitochondrial genome is mitochondrial transcription factor A (Tfam); however, the regulation of Tfam expression du...
Article
The purpose of this study was to investigate whether p53 regulates mitochondrial function via changes in mitochondrial protein import, complex IV assembly, or the expression of key proteins involved in mitochondrial dynamics and degradation. Mitochondria from p53 KO mice displayed ultra-structural alterations, and were more punctate in appearance....
Article
Full-text available
An acute bout of exercise activates downstream signaling cascades that ultimately result in mitochondrial biogenesis. In addition to inducing mitochondrial synthesis, exercise also triggers the removal of damaged cellular material via autophagy, and of dysfunctional mitochondria through mitophagy. Here we investigated the necessity of p53 for the c...
Article
Several studies have illustrated that the polymerase gamma mutator (PolG) mice have reduced mitochondrial content secondary to systemic mitochondrial dysfunction, and subsequently a lower capacity to perform aerobic respiration and endurance exercise. We sought to delineate the extent of glycolysis as a means of energy production in the PolG mice i...
Article
The function Bax and/or Bak in constituting a gateway for mitochondrial apoptosis in response to apoptotic stimuli has been unequivocally demonstrated. However, recent work has suggested that Bax/Bak may have unrecognized non-apoptotic functions related to mitochondrial function in non-stressful environments. Wild type (WT) and Bax/Bak double knock...
Article
Introduction To Muscle Skeletal muscle constitutes ~ 40% of total body weight, and its primary function is to provide force and energy for locomotion, breathing, and postural support. It can also act as a source of heat production during cold-induced stress or exercise. Skeletal muscle is a highly adaptable tissue that exhibits a remarkable range o...
Article
SALEEM, A., H.N. CARTER, S. IQBAL, and D.A. HOOD. Role of p53 within the regulatory network controlling muscle mitochondrial biogenesis. Exerc. Sport Sci. Rev., Vol. 39, No. 4, pp. 199-205, 2011. The tumor suppressor protein p53 is recognized to contribute significantly to the regulation of mitochondrial content. Mice without p53 have reduced endur...
Article
Short-term and longitudinal studies have shown that endurance exercise training extends life expectancy and reduces risk for many chronic disorders, including obesity, insulin resistance and type 2 diabetes (Hawley, 2004). Endurance exercise training orchestrates numerous morphological and metabolic adaptations in skeletal muscle, including mitocho...
Article
Full-text available
Our intent was to investigate the mechanisms driving the adaptive potential of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria in young (6 mo) and senescent (36 mo) animals in response to a potent stimulus for organelle biogenesis. We employed chronic electrical stimulation (10 Hz, 3 h/day, 7 days) to induce contractile activity of ske...
Article
Full-text available
Acute contractile activity of skeletal muscle initiates the activation of signaling kinases. This promotes the phosphorylation of transcription factors, leading to enhanced DNA binding and transcriptional activation and/or repression. The mRNA products of nuclear genes encoding mitochondrial proteins are translated in the cytosol and imported into...
Article
p53 is a tumor suppressor protein that also plays a role in regulating aerobic metabolism. Since skeletal muscle is a major source of whole body aerobic respiration, it is important to delineate the effects of p53 on muscle metabolism. In p53 knockout (KO) mice, we observed diminished mitochondrial content in mixed muscle and lowered peroxisome pro...
Article
Full-text available
During aging, skeletal muscle undergoes sarcopenia, a condition characterized by a loss of muscle cell mass and alterations in contractile function. The origin of these decrements is unknown, but evidence suggests that they can be partly attributed to mitochondrial dysfunction. To characterize the nature of this dysfunction, we investigated skeleta...

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Projects

Project (1)
Project
Epidemiological studies have shown that various modalities of exercise (endurance, resistance, and HIIT) mitigate metabolism-related disorders (such as obesity, type 2 diabetes (T2D), NFLD, etc.) and diseases of aging including cancer, arthritis, dementia, etc. However, the underlying molecular signals that promote crosstalk between organs and orchestrate the pro-metabolic effects of exercise remain unclear. Exercise-induced release of peptides, lipids, nucleic acids, and metabolites from skeletal muscle and other organs (collectively termed ‘exerkines’) has been implicated in mediating these systemic adaptations. Given that the extracellular milieu is not a hospitable environment for labile exerkines, a lipid nanovesicles-based (collectively known as extracellular vesicles) mode of delivery has shaped over the course of evolution for intra- and inter- cellular crosstalk. At least, two types of extracellular vesicles (EVs), exosomes and microvesicles, have been shown to carry various peptides & proteins, nucleic acids (including microRNA, mRNA, and mtDNA), and metabolites that participate in a variety of physiological and pathological processes. Intriguingly, circulatory EVs content increases in an intensity-dependant manner in response to exercise. For this project, our working hypothesis is that the systemic benefits of exercise are modulated by various classes of EVs functioning in an autocrine, paracrine, and/or endocrine manner. Furthermore, we posit that EVs isolated from trained individuals will be enriched with exerkines that will have therapeutic utility in the treatment of metabolic diseases and various aging-associated morbidities. We always welcome collaborators interested in this fascinating project, especially with those with "-omics" expertise.