Axel Thiel

Axel Thiel
French National Centre for Scientific Research | CNRS · Centre of Molecular Genetics

About

10
Publications
425
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227
Citations
Citations since 2016
1 Research Item
151 Citations
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2016201720182019202020212022051015202530
2016201720182019202020212022051015202530
2016201720182019202020212022051015202530

Publications

Publications (10)
Article
Full-text available
The Ori region of bacterial genomes is segregated early in the replication cycle of bacterial chromosomes. Consequently, Ori region positioning plays a pivotal role in chromosome dynamics. The Ori region of the E. coli chromosome is organized as a macrodomain with specific properties concerning DNA mobility, segregation of loci and long distance DN...
Article
Full-text available
Initiation of chromosome segregation in bacteria is achieved by proteins acting near the origin of replication. Here, we report that the precise choreography of the terminus region of the Escherichia coli chromosome is also tightly controlled. The segregation of the terminus (Ter) macrodomain (MD) involves the structuring factor MatP. We characteri...
Data
Tolerance to Large Inversions within a Replichore Nucleoid and cell analyses of strains carrying intrareplichore inversions between NSright and Ori MD (strain Intra O-NSright2), between NSright and Right MD in a strain deleted for TerH and TerI (strain Intra R-NSright3 ΔTerHI), and between NSleft and Left MD (Intra L-NSleft1). Nucleoid and cell ana...
Data
Physiology of the R127-LC13-BO-NSR transposed strain. (A) Cells were grown until OD 0.2 in LB, fixed and DNA stained with DAPI as described before (Esnault et al., 2007). Percentage of cells in each size category according to their number of nucleoids in the wild type strain (left) and the transposed LC13-R127-BONSR strain (right). Coloured horizon...
Data
Imbalance of Replication Arms Nucleoid and cell analyses of strains carrying chromosomes with various levels of imbalance. These inverted configurations were obtained upon interreplichore inversions. For each strain, the genetic map of the chromosome in the inverted configuration is shown. The level of imbalance is indicated above the map, the colo...
Data
Coefficient diffusion of chromosomal markers. (DOCX)
Data
PCR reactions to control the transposition reaction generating strain LC13-R127-BO-NSR. (A) Schematic map of att sites in strain LC13-R127 before and after transposition. To control the result of the transposition reaction, 5 pairs of primers flanking the att sites were used (black arrows). Pairs 1 and 2 amplify respectively the attB and attR sites...
Data
parS tags used in this study. (DOCX)
Data
Long range interactions measured by excisive recombination. (DOC)
Article
Full-text available
The organization of the Escherichia coli chromosome into a ring composed of four macrodomains and two less-structured regions influences the segregation of sister chromatids and the mobility of chromosomal DNA. The structuring of the terminus region (Ter) into a macrodomain relies on the interaction of the protein MatP with a 13-bp target called ma...

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