Aurélien Forget

Aurélien Forget
University of Freiburg | Albert-Ludwigs-Universität Freiburg · Institute of Macromolecular Chemistry

PhD

About

41
Publications
7,799
Reads
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1,562
Citations
Citations since 2016
32 Research Items
1226 Citations
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
Additional affiliations
October 2018 - present
University of Freiburg
Position
  • Akademischer Rat
February 2017 - August 2018
Queensland University of Technology
Position
  • Lecturer
October 2014 - February 2017
University of South Australia
Position
  • Research Associate
Education
October 2009 - February 2014
University of Freiburg
Field of study
  • Macromolecular Chemistry
September 2007 - June 2009
Sorbonne Université
Field of study
  • Polymer Chemistry

Publications

Publications (41)
Preprint
Full-text available
Phycocolloids are gelling polysaccharides extracted from algae that have found applications as microbiological culture media, food thickener, and cosmetic rheological modifiers. The extensive use of algae species that have not been yet domesticated has led to overexploitation or supply shortages. Thus, extending the library of phycocolloids would p...
Preprint
Full-text available
Phycocolloids are gelling polysaccharides extracted from algae that have found applications as microbiological culture media, food thickener, and cosmetic rheological modifiers. The extensive use of algae species that have not been yet domesticated has led to overexploitation or supply shortages. Thus, extending the library of phycocolloids would p...
Article
Full-text available
In multicellular organisms, cells are organized in a 3-dimensional framework and this is essential for organogenesis and tissue morphogenesis. Systems to recapitulate 3D cell growth are therefore vital for understanding development and cancer biology. Cells organized in 3D environments can evolve certain phenotypic traits valuable to physiologicall...
Article
Full-text available
3D-bioprinting (3DBP) possesses several elements necessary to overcome the deficiencies of conventional tissue engineering, such as defining tissue shape a priori, and serves as a bridge to clinical translation. This transformative potential of 3DBP hinges on the development of the next generation of bioinks that possess attributes for clinical use...
Article
Full-text available
Translational Bioinks for 3D Bioprinting 3D‐Bioprinting, a technology at the interface of additive manufacturing, biomaterials and cell biology, is poised to revolutionize regenerative medicine and drug screening. Bioinks are the lynchpin as they bridge bench to bedside. A biology‐based approach to engineering bioinks with pro‐regenerative attribut...
Article
Full-text available
To study biological processes in vitro, biomaterials-based engineering solutions to reproduce the gradients observed in tissues are necessary. We present a platform for the 3D bioprinting of functionally graded biomaterials based on carboxylated agarose, a bioink amendable by extrusion bioprinting. Using this bioink, objects with a gradient of stif...
Article
Full-text available
With the increasing growth of the algae industry and the development of algae biorefinery, there is a growing need for high-value applications of algae-extracted biopolymers. The utilization of such biopolymers in the biomedical field can be considered as one of the most attractive applications but is challenging to implement. Historically, polysac...
Article
Poly(2-alkyl-2-oxazoline) (PAOx) hydrogels are tailorable synthetic materials with demonstrated biomedical applications, thanks to their excellent biocompatibility and tunable properties. However, their use as injectable hydrogels is challenging as it requires invasive surgical procedures to insert the formed hydrogel into the body due to their non...
Article
Full-text available
One of the challenges in 3D-bioprinting is the realization of complex, volumetrically defined structures, that are also anatomically accurate and relevant. Towards this end, in this study we report the development and validation of a carboxylated agarose (CA)-based bioink that is amenable to 3D printing of free-standing structures with high stiffne...
Article
Full-text available
Following ocular surgery, dressings are commonly applied to the surgical wound. These dressings need to combine medical properties with ease of use while maintaining comfort for the patient. For the ocular area, this means that the dressings need to act as a microbial barrier, allow good conformability to the contours of the eye, and provide evapor...
Article
Full-text available
In tissue engineering, cell-adhesion peptides (CAPs) such as the ubiquitous arginine-glycine-aspartic acid (RGD) sequence have allowed the functionalization of synthetic materials to mimic macromolecules of the extracellular matrix (ECM). However, the variety of ECM macromolecules makes it challenging to reproduce all of the native tissue functions...
Article
Full-text available
Porous biodegradable scaffolds have many applications in bioengineering, ranging from cell culture and transplantation, to support structures, to induce blood vessel and tissue formation in vivo. While numerous strategies have been developed for the manufacture of porous scaffolds, it remains challenging to control the spatial organization of the p...
Article
Full-text available
A method is reported for making hollow channels within hydrogels decorated with cell‐adhesion peptides exclusively at the channel surface. Sacrificial fibers of different diameters are used to introduce channels within poly(ethylene glycol) hydrogels crosslinked with maleimide‐thiol chemistry, which are backfilled with a cysteine‐containing peptide...
Article
Full-text available
3D bioprinting as a process has enabled the creation of complex structures laden with biological information including cells that is not realizable with conventional manufacturing techniques. Within this new manufacturing process, design strategies and design tools have to be adapted to deliver its full potential. In architecture, parametric design...
Article
The limited potential of cartilage to regenerate itself has led to development of new strategies and biomaterials for cartilage tissue engineering and regenerative medicine. Although de novo strategies for cartilage repair have been realized, extrudable hydrogels that can be administered in minimally invasive manner while simultaneously supporting...
Article
Full-text available
Systems for efficient and selective capture of micro-scale objects and structures have application in many areas and are of particular relevance for selective isolation of mammalian cells. Systems for the latter should also not interfere with the biology of the cells. This study demonstrates the capture of microspheres through orthogonal coupling u...
Article
Full-text available
Vascularization is a critical step in the restoration of cellular homeostasis. Several strategies including localized growth factor delivery, endothelial progenitor cells, genetically engineered cells, gene therapy, and prevascularized implants have been explored to promote revascularization. But, long‐term stabilization of newly induced vessels re...
Article
Full-text available
In article number 1808050, V. Prasad Shastri and co‐workers describe the formation of a new perfused blood vessel. The stabilization of this neovessel by a mechanically defined hydrogel microenvironment through the recruitment (extravasation) of mechanosensing CD11b⁺/piezo‐1⁺ cells by this microenvironment is depicted against the backdrop of a hema...
Article
Materials that can be actuated by external stimuli are of particular interest for the manufacturing of interactive objects. Such materials open the possibility to change the shape of objects in response to external conditions, such as humidity. Biocompatible and biodegradable materials like agarose and poly(ε-caprolactone)(PCL)are of high interest...
Article
PolyJet three-dimensional (3D) printing allows for the rapid manufacturing of 3D moulds for the fabrication of cross-linked poly(dimethylsiloxane) microwell arrays (PMAs). As this 3D printing technique has a resolution on the micrometer scale, the moulds exhibit a distinct surface roughness. In this study, the authors demonstrate by optical profilo...
Article
Full-text available
Islet transplantation is currently the only minimally-invasive therapy available for patients with type 1 diabetes that can lead to insulin-independence, however it is limited to only a small number of patients. Although clinical procedures have improved in the isolation and culture of islets, a large number of islets are still lost in the pre-tran...
Article
As an alternative to natural extracellular matrix (ECM) macromolecules, cell-adhesion peptides (CAPs) have had tremendous impact on the design of cell culture platforms, implants, and wound dressings. However, only a handful of CAPs have been utilized. The discrepancy in ECM composition strongly affects cell behavior, so it is paramount to reproduc...
Article
Current organ transplantation protocols require the rapid transport of freshly isolated donor tissue to the recipient patient at the site where the procedure is to be conducted. During transport, the tissue graft can quickly deteriorate as a result of oxygen starvation. In this study, we report the fabrication of oxygen-releasing coatings for impro...
Article
This study introduces a thermogelling bioink based on carboxylated agarose (CA) for bioprinting of mechanically defined microenvironments mimicking natural tissues. In CA system, by adjusting the degree of carboxylation, the elastic modulus of printed gels can be tuned over several orders of magnitudes (5–230 Pa) while ensuring almost no change to...
Article
The mechanical properties of agarose-derived hydrogels depend on the scaffolding of the polysaccharide network. To identify and quantify such higher order structure, we applied Raman optical activity (ROA)-a spectroscopic technique that is highly sensitive toward carbohydrates-on native agarose and chemically modified agarose in the gel phase for t...
Article
Cell aggregates reproduce many features of the natural architecture of functional tissues, and have therefore become an important in vitro model of tissue function. In this study, we present an efficient and rapid method for the fabrication of site specific functionalised poly(dimethylsiloxane) (PDMS) microwell arrays that promote the formation of...
Article
The mechanical properties of agarose-derived hydrogels depend on the scaffolding of the polysaccharide network. To identify and quantify such higher order structure, we applied Raman optical activity (ROA)—a spectroscopic technique that is highly sensitive toward carbohydrates—on native agarose and chemically modified agarose in the gel phase for t...
Chapter
Tissue engineering aims to gain mechanistic insights into human diseases and to develop new treatment protocols. Although 2-dimensional (2-D) flat petri dish culture and in vivo disease-based models are the industrial gold standards for understanding the underlying disease pathophysiology and for drug screening/testing, they are associated with cer...
Article
Islet transplantation, the only curative therapy for type I diabetes, requires isolation of the graft in highly specialized facilities for its later dispatch to remote transplantation centres. During transport and culture, many valuable cells are lost due to several factors such as mechanical stress, islet aggregation and dissociation. Here, we eva...
Article
Hydrogel forming polysaccharides, such as the seaweed derived agarose, are well suited for wound dressing applications as they have excellent cell and soft tissue compatibility. For wound dressings, fibrous structure is desirable as the high surface area can favor adsorption of wound exudate and promote drug delivery. Although electrospinning offer...
Article
pH-sensitive hydrogels play an important role in controlled drug release applications and have the potential to impact the management of wounds. In this study, we report the fabrication of novel carboxylated agarose/tannic acid hydrogel scaffolds cross-linked with zinc ions for the pH-controlled release of tannic acid. The resulting hydrogels exhib...
Article
There is mounting evidence that the mechanical property of tissues provides important cues that control cell fate. However, implementation of hydrogels with tunable physicochemical properties is limited due to the challenges associated with crosslinking chemistries. It has been recently shown that mechanically well-defined injectable polysaccharide...
Article
Full-text available
Mechanical aspects of the cellular environment can influence cell function, and in this context hydrogels can serve as an instructive matrix. Here we report that physicochemical properties of hydrogels derived from polysaccharides (agarose, κ-carrageenan) having an α-helical backbone can be tailored by inducing a switch in the secondary structure f...
Article
When polymeric nanoparticles (NPs) are formed by nanoprecipitation, which is a nucleation-growth process, the control over size requires changing the polymer concentration or solvent composition. Here we demonstrate that the NP size can be controlled independent of polymer variables by introducing a polyelectrolyte (PE) in the aqueous phase. PEs th...
Thesis
Full-text available
The thesis focuses on the development of a system that recapitulates the five criteria for a successful translatable synthetic extra cellular matrix (ECM): 1/ the control of the mechanical environment of the tissue, 2/ the controlled release of soluble signals, 3/ the transduction of the mechanical cues to the cells through immobilized signals, 4/...
Article
The template-free Ionothermal synthesis of porous polymer/carbon-materials, leading to nonperiodic, but well-defined mesoporosity with a broad range of pore sizes and favorable pore connectivity was studied. The experiment included the storage of Zinc chloride in a glove box, whose elemental analysis was obtained from a Various El elemental analyze...
Article
High surface area organic materials featuring both micro- and mesopores were synthesized under ionothermal conditions via the formation of polyaryltriazine networks. While the polytrimerization of nitriles in zinc chloride at 400 degrees C produces microporous polymers, higher reaction temperatures induce the formation of additional spherical mesop...

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Projects

Projects (3)
Project
Utilization the amendable physicochemical properties of carboxylated agarose to develop hydrogel and polysaccharide based solutions to current technological challenges
Project
A series of interdisciplinary workshops with designers and scientists to identify a 21st Century design experience based on the concept of Morphogenetic Prototyping. The workshops will be held in the week of November 21st 2016.