Astrid Böhne

Astrid Böhne
Research Museum Alexander Koenig | ZFMK · Center for Molecular Biodiversity Research (zmb)

PhD

About

71
Publications
14,534
Reads
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969
Citations
Additional affiliations
January 2011 - present
University of Basel
Position
  • Sex determination in cichlids
January 2007 - December 2010
Ecole normale supérieure de Lyon
January 2007 - December 2010
Institut de Génomique Fonctionnelle,

Publications

Publications (71)
Article
Full-text available
Background Sex chromosomes change more frequently in fish than in mammals or birds. However, certain chromosomes or genes are repeatedly used as sex determinants in different members of the teleostean lineage. East African cichlids are an enigmatic model system in evolutionary biology representing some of the most diverse extant vertebrate adaptiv...
Article
Full-text available
Background The factors determining sex are diverse in vertebrates and especially so in teleost fishes. Only a handful of master sex-determining genes have been identified, however great efforts have been undertaken to characterize the subsequent genetic network of sex differentiation in various organisms. East African cichlids offer an ideal model...
Article
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Males and females of the same species differ largely in gene expression, which accounts for most of the morphological and physiological differences and sex-specific phenotypes. Here, we analyzed sex-specific gene expression in the brain and the gonads of cichlid fishes from Lake Tanganyika belonging to four different lineages, so-called tribes (Ere...
Article
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Teleost fishes are the most species-rich clade of vertebrates and feature an overwhelming diversity of sex-determining mechanisms, classically grouped into environmental and genetic systems. Here, we review the recent findings in the field of sex determination in fish. In the past few years, several new master regulators of sex determination and ot...
Article
Full-text available
Sex determination mechanisms are highly variable across teleost fishes and sexual development is often plastic. Nevertheless, downstream factors establishing the two sexes are presumably conserved. Here, we study sequence evolution and gene expression of core genes of sexual development in a prime model system in evolutionary biology, the East Afri...
Article
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The unique male pregnancy in pipefishes and seahorses ranges from basic attachment (pouch-less species: Nerophinae) of maternal eggs to specialized internal gestation in pouched species (e.g. Syngnathus and Hippocampus) with many transitions in between. Due to this diversity, male pregnancy offers a unique platform for assessing physiological and m...
Article
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The evolution of pregnancy exposes parental tissues to new, potentially stressful conditions, which can trigger inflammation.1 Inflammation is costly2,3 and can induce embryo rejection, which constrains the evolution of pregnancy.1 In contrast, inflammation can also promote morphological innovation at the maternalembryonic interface as exemplified...
Article
Cichlids are well known for their propensity to radiate generating arrays of morphologically and ecologically diverse species in short evolutionary time. Following this rapid evolutionary pace, cichlids show high rates of sex chromosome turnover. We here studied the evolution of sex-biased gene (SBG) expression in 14 recently diverged taxa of the L...
Article
Sex is a fundamental trait determined by environmental and/or genetic factors, including sex chromosomes. Sex chromosomes are studied in species scattered across the tree of life, yet little is known about tempo and mode of sex chromosome evolution among closely related species. Here, we examine sex chromosome evolution in the adaptive radiation of...
Article
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Changes in gene expression play a fundamental role in phenotypic evolution. Transcriptome evolutionary dynamics have so far mainly been compared among distantly related species and remain largely unexplored during rapid organismal diversification, in which gene regulatory changes have been suggested as particularly effective drivers of phenotypic d...
Article
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Adaptive radiation is the likely source of much of the ecological and morphological diversity of life1–4. How adaptive radiations proceed and what determines their extent remains unclear in most cases1,4. Here we report the in-depth examination of the spectacular adaptive radiation of cichlid fishes in Lake Tanganyika. On the basis of whole-genome...
Article
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Cichlid fishes are celebrated for their vast taxonomic, phenotypic, and ecological diversity; however, a central aspect of their evolution - the timeline of their diversification - remains contentious. Here, we generate draft genome assemblies of 14 species representing the global cichlid diversity and integrate these into a new phylogenomic hypoth...
Article
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Variation in gene expression contributes to ecological speciation by facilitating population persistence in novel environments. Likewise, immune responses can be of relevance in speciation driven by adaptation to different environments. Previous studies examining gene expression differences between recently diverged ecotypes have often relied on on...
Preprint
Full-text available
Sex is a fundamental trait that is determined, depending on the species, by different environmental and/or genetic factors, including various types of sex chromosomes. However, while the functioning and evolution of sex chromosomes have been explored in species scattered across the eukaryotic tree of life, little is known about tempo and mode of se...
Article
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Background: Differential gene expression can be translated into differing phenotypic traits. Especially during embryogenesis, specific gene expression networks regulate the development of different body structures. Cichlid fishes, with their impressive phenotypic diversity and propensity to radiate, are an emerging model system in the genomics era...
Article
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Why sex chromosomes turn over and remain undifferentiated in some taxa, whereas they degenerate in others, is still an area of ongoing research. The recurrent occurrence of homologous and homomorphic sex chromosomes in distantly related taxa suggests their independent evolution or continued recombination since their first emergence. Fishes display...
Article
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When trying to find a mate, one might think about using a dating app. Imagine that someone else has installed the same app and tries to access the same potential mates that you have chosen, and that this someone uses false facts about him/herself to increase the chance of dating someone before you or anyone else with honest status information does....
Article
Disentangling the processes and mechanisms underlying divergent selection in diversification is facilitated by the comparative study of replicate population pairs that have diverged along a similar environmental gradient. Such a setting is realized in a cichlid fish from southern Lake Tanganyika, Astatotilapia burtoni, which occurs both within the...
Article
Sex steroids are major drivers of sexual development and also responsible for the maintenance of the established gender. Especially fishes exhibit great plasticity and less conservation in sex determination and sexual development compared to other vertebrate groups. In addition, fishes have a constant sex steroid production throughout their entire...
Article
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The emergence of the steroid system is coupled to the evolution of multicellular animals. In vertebrates in particular, the steroid receptor repertoire has been shaped by genome duplications characteristic to this lineage. Here, we investigate for the first time the composition of the androgen receptor signaling pathway in ray-finned fish genomes f...
Article
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The origin of novel phenotypic characters is a key component in organismal diversification; yet, the mechanisms underlying the emergence of such evolutionary novelties are largely unknown. Here we examine the origin of egg-spots, an evolutionary innovation of the most species-rich group of cichlids, the haplochromines, where these conspicuous male...
Article
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In two swordtail species of the genus Xiphophorus, the onset of puberty has been shown to be modulated at the P locus by sequence polymorphism and gene copy number variation affecting the type 4 melanocortin hormone receptor Mc4r. The system works through the interaction of two allelic types, one encoding wild-type, and the other dominant-negative...
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Conference Paper
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Background / Purpose: The East African Great Lakes Tanganyika, Malawi and Victoria are home to one of the most fascinating examples of adaptive radiations: more than 2000 endemic cichlid species evolved in a few million years only, making these cichlid species-flocks prime model systems in evolutionary biology. Yet, the abiotic and biotic factors...
Article
Teleost fish genomes display remarkable features, possibly linked to the impressive biodiversity observed in these animals. An event of genome duplication has taken place in the ray-finned fish lineage before teleost radiation. From this event, thousands of genes have been kept as duplicates, with frequent divergent evolution in different teleost s...
Article
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Transposable elements are widespread mobile DNA sequences able to integrate into new locations within genomes. Through transposition and recombination, they significantly contribute to genome plasticity and evolution. They can also regulate gene expression and provide regulatory and coding sequences (CDSs) for the evolution of novel gene functions....
Article
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Members of the makorin (mkrn) gene family encode RING/C3H zinc finger proteins with U3 ubiquitin ligase activity. Although these proteins have been described in a variety of eukaryotes such as plants, fungi, invertebrates and vertebrates including human, almost nothing is known about their structural and functional evolution. Via partial sequencing...
Article
A consensus sequence, encoding a putative DNA polymerase type B derived from a Polinton transposon, was assembled from the sex determination region of Xiphophorus maculatus. This predicted protein, which is 1,158 aa in length, contains a DNA_pol_B_2 domain and a DTDS motif. The DNA polymerase type B gene has about 10 copies in the haploid X. macula...
Article
In contrast to mammals and birds, fish display an amazing diversity of genetic sex determination systems, with frequent changes during evolution possibly associated with the emergence of new sex chromosomes and sex-determining genes. To better understand the molecular and evolutionary mechanisms driving this diversity, several fish models are studi...
Article
In an attempt to study the sex-determining region in the platyfish Xiphophorus maculatus bacterial artifical chromosome (BAC)-Contigs have been assembled covering the sex-determining region on the X and the Y chromosome. Rearrangements, characterized by duplication or inversions, have been noticed in the genomic structure of this region. Sequencing...
Article
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Poeciliids, a family of live-bearing freshwater fish, including among others platyfish, swordtails and guppies, fully illustrate the diversity of genetic sex determination mechanisms observed in teleosts. Besides unisexuality, a variety of sex-determining systems has been described in this group of fish, including male and female heterogamety with...
Chapter
The development of high-throughput DNA sequencing methods has opened the era of genomics, which has revolutionized biology, medicine, and biotechnology over the last decade. The rise of genomics has generated an impressive wave of novel information concerning genome structure, function, and evolution. Massive analysis of functional gene variability...
Article
Full-text available
In order to better understand the molecular and evolutionary mechanisms driving sex determination in fish, bacterial artificial chromosome (BAC) contigs covering the sex determination region of the X and Y sex chromosomes of the platyfish Xiphophorus maculatus have been constructed. Initial analysis of these contigs led to the identification of ele...
Article
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Comparative genomics has revealed that major vertebrate lineages contain quantitatively and qualitatively different populations of retrotransposable elements and DNA transposons, with important differences also frequently observed between species of the same lineage. This is essentially due to (i) the differential evolution of ancestral families of...
Article
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Due to the presence of genetically well-defined sex chromosomes, with a relatively restricted sex-determination region containing markers identified at the molecular level, the platyfish Xiphophorus maculatus is one of the best models for the positional cloning of a master sex-determining gene in fish. Both male and female heterogametes and three d...

Projects

Projects (2)
Project
1) Relative importance in wild populations of the Nile tilapia 2) Mechanisms 3) Consequences
Project
Sex determination is not conserved in fish and different mechanisms and genes act to determine the sex. I investigate this in the largest adaptive radiation of vertebrates, the fascinating cichlids from East African Lake Tanganyika.