Assist. Prof. Dr. Sevan O. MajedSalahaddin University - Erbil | SUH · College of Education
Assist. Prof. Dr. Sevan O. Majed
Doctor of Philosophy
About
19
Publications
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44
Citations
Introduction
Sevan Majed currently works at the College of Education, Salahaddin University - Erbil. Sevan does research in Evolutionary Biology, Cancer Research and Bioinformatics. Their most recent publication is 'Phylogenetic Relationship among Phosphomannose Isomerase (PMI), Phosphomannose Mutase (PMM) and GDP-D-Mannose Pyrophosphorylase (GMP) in Photosynthetic Eukaryotes'.
Publications
Publications (19)
Abnormally expressed microRNAs (miRNAs) have been observed to interfere with intracellular RNA mechanisms in breast cancer (BC). Small RNA sequencing of 50 BC and 50 normal adjacent tissue (NAT) was conducted to identify the differential expression of non-coding transcripts. The findings showed that 152 tumor-suppressive and 22 oncogenic miRNAs wer...
Introduction: Since its isolation in the UK, the SARS-CoV-2 Delta variant has become an epidemic. This study aimed to decipher the viral appearance and genomic characterization of the Delta variant isolated from patients in the Kurdistan region of Iraq. Methodology: Samples were collected from the West Erbil Emergency Hospital, and infection by SAR...
Introduction: Coronavirus disease 2019 (COVID-19) first appeared in Iraq, including the Iraqi Kurdistan region governorates, in March 2020. Methodology: 48,494 samples were collected from public hospitals in the Kurdish governates from February 2021 to May 2022. Viral RNA was extracted, and real time quantitative polymerase chain reaction (RT-PCR)...
In this study, early adverse impacts that emerged after vaccination with each dose of these vaccines were compared from previously infected participants. Ant-SARS-CoV-2 spike-specific IgG and IgA antibodies produced by these three vaccines have been assessed using ELISA method at different time periodsꓼ including pre-vaccination, 25 days after the...
MicroRNA that is abnormally produced in breast cells can disrupt biological processes, which can lead to cancer. This study aims to screen differentially expressed genes (DEGs) and ncRNAs (DEncRNAs) in the formalin-fixed paraffin-embedded (FFPE) tissues of breast cancer (BC) as compared with the normal adjacent tissues (NAT), and identify miR-4510...
Citation: Majed, S.O.; Mustafa, S.A.; Jalal, P.J.; Fatah, M.H.; Miasko, M.; Jawhar, Z.; Karim, A.Y. SARS-CoV-2 Omicron Variant Genomic and Abstract: Omicron variants have been classified as Variants of Concern (VOC) by the World Health Organization (WHO) ever since they first emerged as a result of a significant mutation in this variant, which show...
This study aims to screen the differential expression of total RNA transcripts in formalin-fixed paraffin-embedded tissues (FFPETs) in breast cancer (BRCA) and normal adjacent tissues (NATs) and identify miR-191 as a new biomarker for early diagnosing BRCA. Differentially expressed genes (DEGs) by MACE-Seq and differentially expressed ncRNAs (DEncR...
Introduction
Disruption of cellular processes in the breast by abnormally expressed miRNA is characterized to develop cancer. We aimed to identify the differential expression of small RNAs (sRNAs) and mRNAs in formalin-fixed paraffin-embedded (FFPE) tissue of the breast cancer (BC) and normal adjacent tissue (NAT). Another aim is to determine the d...
The new emerging coronavirus SARS-CoV2 is an alerting pandemic worldwide. Understanding the epidemiology, viral behaviour in the host and the severity of the disease in an infected patient is a demanding approach for the healthcare system which lead to plan and contemplate the response for further waves of the same virus and even other related viru...
The new emerging coronavirus SARS-CoV2 is an alerting pandemic worldwide. Understanding the epidemiology, viral behavior in the host, and the severity of the disease in an infected patient is a demanding approach for the healthcare system which lead to plan and contemplate the response for further waves of the same virus and even other related viru...
Introduction: Disruption of cellular processes in the breast by abnormally expressed miRNA is characterized to develop cancer. We aimed to identify the differential expression of small RNAs (sRNAs) and mRNAs in formalin-fixed paraffin-embedded (FFPE) tissue of the breast cancer (BC) and normal adjacent tissue (NAT). Another aim is to determine the...
Introduction
Disruption of cellular processes in the breast by abnormally expressed miRNA is characterized to develop cancer. We aimed to determine the differential expression of coding and non-coding RNAs in formalin fixed paraffin embedded (FFPE) blocks of breast cancer (BC) tissue and normal adjacent tissue (NAT). Another aim is to determine dif...
Phosphomannose isomerase (PMI), phosphomannose mutase (PMM) and GDP-D-mannose pyrophosphorylase (GMP) are three key enzymes in the regulation of the initial steps of the L-ascorbate biosynthesis pathway. They are widely distributed in lineages of vascular plants but hardly found in non-vascular plants. It is hypothesized that there is an evolutiona...
Phosphomannose isomerase (PMI), phosphomannose mutase (PMM) and GDP-D-mannose pyrophosphorylase (GMP) are three key enzymes in the regulation of the initial steps of the L-ascorbate biosynthesis pathway. They are widely distributed in lineages of vascular plants but hardly found in non-vascular plants. It is hypothesized that there is an evolutiona...
(The occurrence of L-GALACTONO
GalLDH) in L-ascorbate biosynthesis pathway from photosynthetic
eukaryotes)
Abstract: L-galactose dehydrogenase (L-GalDH) in photosynthetic eukaryotes is acting as a key enzyme
in the pathway of L-ascorbate biosynthesis. This enzyme is widely distributed in most lineages
of photosynthetic eukaryotes. From this study, multiple sequence alignment inferred that there
is an evolutionary relationship amongst the L-GalDH sequenc...
L-Galactono-1, 4-lactone dehydrogenase (L-GalLDH) is a key enzyme that catalyzes the last step in the L-ascorbate pathway in biosynthetic eukaryotes. It is widely distributed in the different lineages of photosynthetic eukaryotes. Here, we hypothesized that there is a close evolutionary relationship between L-GalLDHsequences of these eukaryotic lin...
Questions
Questions (21)
I have FFPE breast tissue, I want to extract protein from it using RIPA lysis buffer? is the RIPA buffer great for the FFPE tissue?
Hi friends
Who knows about Kaplan Meier analysis. I have gene expression data. I want to design the Kaplan Meier curve. If somebody knows, let me know,
Dear/Madam
I am a phD Student I have a kaplan meire data for patient survival. I would like to analyse them.
If you have any software or website, please let me knwo
Hi
I want to design miRNA targets and schematic of miRNA targets for microRNA. I need a great software. Does anyone has it? if you have any software, please could you give me?
Best Regards
Sevan
Dear Sir/Madam
I know there are some websites giving you the target predicted for microRNA. I am a little confused about them. Can anyone has a reliable websites that is helpful to get the best target for microRNAS?
BEST REGARDS
PhD student SEVAN
Dear Sir/Madam
I want to read this article if someone has can you send to me?
Downregulated miR-1247-5p associates with poor prognosis and facilitates tumor cell growth via DVL1/Wnt/β-catenin signaling in breast cancer
I need this software for PhD data analysis. Please if you have this software, I will be grateful?
Dear/ Madam
I am a little confused about extraction of microRNA from FFE block. whether total RNA is first extracted from FFE tissue and then miRNA is extracted from total RNA or Can I synthesize single strand cDNA for miRNA can be synthesised from total RNA.
Dear/Madam
I want to extract microRNA from FFPE tissue. I have used Norgen Kits for miRNA purification but my result is not good when I run Real Time PCR. I donot know why? . I think I have had some contamination. Can you give me some advise. In the protocol, DNase I is not mentioned but I know DNase I is great to break the remaining DNA. Can I use that solution during washing miRNA Enrichment Column? If it is ok, How much concentration should I use?
Dear Madam
I need a good protocol for MicroRNA purification from FFPE-breast tissue. please If anybody has it, can you send it to me.
Dear/Madam
I am a Ph.D. student. I am working on microRNA expression in FFPE breast tissue. Today I did a pretest for one sample (Cancer) to detect one microRNA. I ran a breast cancer sample and negative control. The amplification plot is then designed like the attached file. Can anybody explain that for me? Is this a good result? will I continue at the same procedure?
I have no idea about data analysis of RT-qPC. Can somebody tell me this plot is good or not?
Dear/Madam
I need this article "Extraction of miRNAs from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues". If somebody has it, please send me.
Best Regards
Sevan
