Ashwini Kamble

The Energy and Resources Institute | TERI · Center for Environmental Studies

Many international organizations are trying to put a lot of efforts and money for gender sensitisation in all the fields. Media is more important because it reflects the people's opinions and expectations. UNESCO is the one such organisation. It has introduced Gender Sensitive Indicators for Media to address gender issues in media houses and in the...

p>The concept of laboratory rat is giving way to the computer mouse arose after the famous handshake between Clinton-Blair for the completion of the human genome in April 2003. Bioinformatics is defined as the application of computational techniques to understand and organize the information associated with biological macromolecules. There is avai...

Immobilized cells of a recombinant Escherichia coli expressing nitrilase from Pseudomonas putida were used to catalyze the hydrolysis of mandelonitrile (2-hydroxy-2-phenylacetonitrile) to (R)-(−)-mandelic acid. The cells had been immobilized by entrapment in an alginate matrix. Conditions for the hydrolysis reaction were optimized in shake flasks a...

The intracellular cobalt-type nitrile hydratase was purified from the bacterium Rhodococcus erythropolis. The pure enzyme consisted of two subunits of 29 and 30 kDa. The molecular weight of the native enzyme was estimated to be 65 kDa. At 25 °C the enzyme had a half-life of 25 h. The Michaelis–Menten constants K m and v max for the enzyme were 0.62...

Effects of medium pH (uncontrolled and controlled), aeration rate and agitation intensity on the production of biomass and nitrilase by a recombinant Escherichia coli in a stirred-tank bioreactor are reported. The recombinant bacterium expressed the nitrilase gene of Alcaligenes faecalis. The initial pH of the culture medium had a strong influence...

To evaluate the effect of different physicochemical parameters such as agitation, aeration and pH on the growth and nitrile hydratase production by Rhodococcus erythropolis MTCC 1526 in a stirred tank reactor. Rhodococcus erythropolis MTCC 1526 was grown in 7-l reactor at different agitation, aeration and controlled pH. The optimum conditions for b...

The cross-linked enzyme aggregate (CLEA) method is used for the dual purpose of combining both the purification and immobilization of enzyme in one step. The present work involved the preparation of a carrier-free, highly active reusable biocatalyst (nitrilase) which encounters least mass-transfer limitations with higher thermal and storage stabili...

The reaction conditions for the nicotinamide production by Rhodococcus erythropolis MTCC 1526 have been optimized by statistical experimental design. Application of this approach in the bioprocess can result in rallied product yield, reduced development time, and process variability. In this investigation, response surface methodology and central c...

Culture conditions were optimized for the growth and carbonyl reductase production by a novel yeast strain Candida viswanathii. Response surface methodology was applied for the critical medium components (initial pH, mannitol, yeast extract and calcium chloride) identified earlier by one-factor-at-a-time approach. Central composite design was used...

A new yeast strain Candida viswanathii MTCC 5158, isolated from soil, is capable of carrying out the enantioselective reduction of 1-acetonaphthone to S(−)-1-(1′-naphthyl) ethanol which is an important synthetic intermediate of mevinic acid analogue, (potential inhibitor of 3-hydroxy methyl glutaryl coenzyme A reductase). Studies on the culture con...