Ashraf Al Ashhab

Dead Sea-Arava Science Center (DSASC) | DSASC · The Dead Sea

Biofouling is the main challenge in the operation of anaerobic membrane bioreactors (AnMBRs). Biofouling strongly depends on temperature; therefore, we hypothesize that the interactions and viscoelastic properties of soluble microbial products (SMP) and extracellular polymeric substances (EPS) vary with temperature, consequently influencing membran...

Freshwater bodies are critical components of terrestrial ecosystems. The microbial communities of freshwater ecosystems are intimately linked water quality. These microbes interact with, utilize and recycle inorganic elements and organic matter. Here, we present three metagenomic sequence datasets (total of 182.9 Gbp) from different freshwater envi...

Antiscalants are organic polymers widely used for scale inhibition in seawater desalination. While they are susceptible to biodegradation, they provide nutrients for bacterial cell growth and energy for the microbes that assimilate and degrade them. This paper shows the biodegradability of three commercial antiscalants (polyacrylate—CA, polyphospho...

Background The welfare of farmed fish is influenced by numerous environmental and management factors. Fish skin is an important site for immunity and a major route by which infections are acquired. The objective of this study was to characterize bacterial composition variability on skin of healthy, diseased, and recovered Gilthead Seabream (Sparus...

Background: The welfare of farmed fish is influenced by numerous environmental and management factors. Fish skin is an important site for immunity and a major route by which infections are acquired. The objective of this study was to characterize bacterial composition variability on skin of healthy, diseased and recovered Gilthead Seabream (Sparus...

Background: The evolutionary relationships between plants and their microbiomes are of high importance to the survival of plants in general and even more in extreme conditions. Changes in the plant's microbiome can affect plant development, growth, fitness, and health. Along the arid Arava, southern Israel, acacia trees ( Acacia raddiana and Acacia...

Aquaculture facilities such as fishponds are one of the most anthropogenically impacted freshwater ecosystems. The high fish biomass reared in aquaculture is associated with an intensive input into the water of fish-feed and fish excrements. This nutrients load may affect the microbial community in the water, which in turn can impact the fish healt...

In the oceans and seas, environmental conditions change over multiple temporal and spatial scales. Here, we ask what factors affect the bacterial community structure across time, depth and size fraction during six seasonal cruises (two years) in the ultra‐oligotrophic Eastern Mediterranean Sea. The bacterial community varied most between size fract...

Aquaculture facilities such as fishponds are one of the most anthropogenically impacted freshwater ecosystems. The high fish biomass reared in aquaculture is associated with an intensive input into the water of fish-feed and fish excrements. This nutrients load may affect the microbial community in the water, which in turn can impact the fish healt...

Excessive use of antimicrobials in aquaculture is concerning, given possible environmental ramifications and the potential contribution to the spread of antimicrobial resistance (AR). In this study, we explored seasonal abundance of antimicrobial resistance genes and bacterial community composition in the water column of an intensive aquaculture po...

micrometre importance in arid environments

The effect of dietary omega-6 long-chain polyunsaturated fatty acid (LC-PUFA) on host microbiome and gut associated immune function in fish is unexplored. The effect of dietary supplementation with the omega-6 LC-PUFA-rich microalga Lobosphaera incisa wild type (WT) and its delta-5 desaturase mutant (MUT), rich in arachidonic-acid and dihomo-gamma-...

In the oceans and seas, environmental conditions change over multiple temporal and spatial scales. Here, we ask what factors affect the bacterial community structure across time, depth and size fraction during six seasonal cruises (two years) in the ultra-oligotrophic Eastern Mediterranean Sea. The bacterial community varied most between size fract...

Background: The evolutionary relationships between plants and their microbiome are of high importance to the survival of plants in extreme conditions. Changes in microbiome of plants can affect plant development, growth and health. Along the arid Arava, southern Israel, acacia trees ( Acacia raddiana and Acacia tortilis ) are considered keystone sp...

Background: The evolutionary relationships and interactions between plants and their microbiomes are of high importance to the survival of plants in extreme conditions. Changes in the plant’s microbiome can affect plant development, growth and health. Along the arid Arava, southern Israel, acacia trees (Acacia raddiana and Acacia tortilis) are cons...

Lakes and other freshwater bodies are intimately connected to the surrounding land, yet to what extent land-use affects the quality of freshwater and the microbial communities living in various freshwater environments is largely unknown. We address this question through an analysis of the land use surrounding 46 inter-connected lakes located within...

Along the arid Arava, southern Israel, acacia trees ( Acacia raddiana and Acacia tortilis ) are considered keystone species. In this study, we investigated the ecological effects of plant species, microclimate (different areas within the tree canopies) and seasonality on the endophytic and epiphytic microbiome associated with these two tree species...

AbstractWater quality, agriculture and human health are inextricably linkedthrough aquatic microbial populations. Harmful Algal Blooms (HABs) areone of the main reasons for reduced water quality worldwide, and canespecially impact regions with limited fresh-water supplies such as theparts of the Mediterranean and the Middle East. To gain a better u...

Ancient agricultural stone terraces, dated to the Roman and Byzantine ages, are prevalent across the Negev drylands of Southern Israel. The goal of these structures was to reduce hydrological connectivity by harvesting water runoff and controlling soil erosion, thus allowing cultivation of cereals. Land abandonment and the lack of maintenance have...

Aquatic microbial populations are intimately connected with the environmental conditions within their host water body, yet how they are affected by the surrounding land use (terrestrial-aquatic coupling) or by connectivity and transport between water bodies is less clear. We address these questions through an analysis of the land use surrounding 46...

While tillage of agricultural lands has been used extensively, its utilization for restoring degraded semi-natural lands is rare. This study was conducted in the arid southern Israel in a shrubland which has faced severe degradation processes over time, including soil erosion and compaction, and negation of vegetation recovery. In 2014, research pl...

The objective of this study was to characterize skin bacterial variability in healthy and sick gilthead seabream (Sparus aurata), using a high-throughput DNA sequencing method. We compared the fish skin microbiome in a controlled environment with UV-treated water and non UV-treated water in both healthy and sick conditions. Swabs samples were colle...

Dead Sea climatotherapy (DSC) is a therapeutic modality for a variety of chronic skin conditions, yet there has been scarce research on the relationship between the cutaneous microbiota and disease states in response to DSC. We characterized the skin bacterial and fungal microbiome of healthy volunteers who underwent DSC. Bacterial community divers...

Laboratory-scale reverse osmosis (RO) flat-sheet systems were used with two parallel flow cells, one treated with cleaning agents and a control (ie undisturbed). The cleaning efforts increased the affinity of extracellular polymeric substances (EPS) to the RO membrane and altered the biofilm surface structure. Analysis of the membrane biofilm commu...

We investigated the influence of feed-water shear rate during reverse-osmosis (RO) desalination on biofouling with respect to microbial community composition developed on the membrane surface. The RO membrane biofilm's microbial community profile was elucidated during desalination of tertiary wastewater effluent in a flat-sheet lab-scale system ope...

Bacteriocins produced by Enterobacteriaceae are high molecular weight toxic proteins that kill target cells through a variety of mechanisms, including pore formation and nucleic acid degradation. What is remarkable about these toxins is that their expression results in death to the producing cells and therefore bacteriocin induction have to be tigh...

Reverse-osmosis (RO) desalination is frequently used for the production of high-quality water from tertiary treated wastewater (TTWW). However, the RO desalination process is often hampered by biofouling, including membrane conditioning, microbial adhesion, and biofilm growth. The vast majority of biofilm exploration concentrated on the role of bac...

Microbial communities in soils may change in accordance with distance, season, climate, soil texture and other environmental parameters. Microbial diversity patterns have been extensively surveyed in temperate regions, but few such studies attempted to address them with respect to spatial and temporal scales and their correlations to environmental...

PCR primers and procedures used in this study. (DOCX)

Comparison of three restriction enzymes (RE) for TRFLP analysis using each of the five microbial markers. For each RE, the power to differentiate between sites was analyzed as the A-statistic of the MRPP test. For each marker, the best RE (i.e. highest A) was set as 100%, and the differentiation power of the other two RE was calculated as percentag...

Abundance of α-proteobacterial, actinobacterial and bacterial 16S rRNA. Values are mean and standard deviation (SD) of two qPCR measurements in each season. (DOCX)

Soil may constitute physical evidence that connects people to geographic locations. We studied several parameters of molecular fingerprinting of microbes from arid soils, revealing that (i) for extraction of soil DNA, the 'total nucleic acid' method provides higher spatial and temporal resolution than the PowerSoil® method; (ii) denaturing gradient...

The exploration of spatial patterns of abundance and diversity patterns along precipitation gradients has focused for centuries on plants and animals; microbial profiles along such gradients are largely unknown. We studied the effects of soil pH, nutrient concentration, salinity, and water content on bacterial abundance and diversity in soils colle...