Ashok Bhatia

Ashok Bhatia
GLA University · biotechnology

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76
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582
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Publications

Publications (76)
Article
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Over the years, biodegradation has been an effective technique for waste water treatment; however, it has its own limitations. In order to achieve a higher degradation efficacy, integrated processes are being focus in this area. Therefore, the present study is targeted towards the coupling of biodegradation and photocatalytic degradation of p-creso...
Article
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Johne's disease (JD), caused by Mycobacterium avium subspecies paratuberculosis (MAP), is endemic in livestock leading to low per animal productivity. MAP as survives pasteurization, poses a public health problem because of high exposure to animals and humans. There is an urgent need for newer diagnostic tests with high specificity and sensitivity...
Article
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Foot-and-mouth disease (FMD) is an important transboundary disease affecting domestic and wild ruminants. Due to FMD outbreaks, the annual economic losses in endemic countries range from USD 6.1 billion to 200 billion. It also restricts the export of animals/animal by-products to FMD-free countries. FMD-free countries can experience a more severe e...
Article
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High endemicity of Johne’s disease (JD) in herds adversely affects heavy milk yielding breeds by reducing the per animal productivity and ‘productive life-span’.This review evaluates different vaccines used for its control and summarizes the benefits of ‘global vaccine’ in the four major domestic livestock species, namely goat, sheep, buffalo and c...
Article
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Immunomodulatory activity of hot aqueous extract of Neolamarckia cadamba leaves with particular reference to splenocyte proliferation and induction of cytokines has been investigated using Wistar albino rat model. Study of hot aqueous extract (HAE) of N. cadamba leaves over cell mediated immune response was carried out using six groups (Gp-I as con...
Article
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Epilepsy is one of the most common neurological disorders and there is a pressing need to explore therapeutics based on Complementary and Integrative system of medicines. The present study was designed to validate the protective action of a homeopathic medicine Nux vomica on mice in experimental epilepsy, and also to elucidate its probable mechanis...
Article
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Tuberculosis meningitis (TBM) is the most common form of chronic infection of central nervous system. Regardless of the extent of the problem, the general diagnostic outlook is discouraging specifically; there is no generally accepted early confirmative diagnostic protocol available for TBM. In the current work, 16 Cerebrospinal Fluid (CSF) cases w...
Article
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Monitoring tuberculosis (TB) treatment success is crucial for clinical decision-making. The only available tool in this regard is sputum microscopy, but it has demerits. Moreover, in case of smear negatives and extrapulmonary TB, an efficient tool is still sought for. Therefore, we evaluated T regulatory cell (Treg)-associated markers (CD25, CD39,...
Article
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Introduction Nanotechnology is a branch of nanosciences which includes the manipulation of particles at their atomic level in order to obtain certain desirable properties that enhance their use in the treatment of various metabolic disorders as well as other life-threatening diseases including cancer. Photosynthesized nanoparticles are eco-friendly...
Article
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Neolamarckia cadamba (Roxb.) Bosser syn. Anthocephalus cadamba (Roxb.) Miq., an evergreen tropical tree, known in Hindu Mythology, for its various medical properties. In the present study, immunomodulatory potential of N. cadamba has been investigated in Wistar albino rat model. The effect of hot aqueous extract (HAE) of N. cadamba leaves over diff...
Article
Johne's disease (JD) is the most widely distributed disease of the domestic livestock species world-wide. In the absence of control programs, bio-load of Mycobacterium aviumsubspecies paratuberculosis (MAP) had shown increasing trends in the Indian goat population over the years. Recently a therapeutic ‘Indigenous vaccine’ has been developed agains...
Article
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Highly versatile and robust 'Indigenous ELISA kit' for the diagnosis of Mycobacterium avium subsp. paratuberculosis infection in cattle herds was compared with 'Ethanol Vortex (EV) ELISA kit' of USA. Of 160 (118 vaccinated and 42 nonvaccinated) cattle screened, 129 and 35 were positive in 'Indigenous' and 'Ethanol Vortex' ELISA kits, respectively....
Article
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Present study estimated status of Johne’s disease in the buffalo population of Malwa region using goat based Indigenous plate ELISA test. Of 156 serum samples screened 41.0% (64) and 85.8% (134) were positive for MAP infection by Indigenous plate ELISA kit; condition (A), condition (B), respectively. Study showed that despite high slaughter rate, p...
Article
Full-text available
Objective:To investigate the antiepileptic effect of homeopathic remedy Nux vomica on mice and its comparison with standard therapeutic diazepam. Methods: BALB-c mice were taken and divided into three groups comprising ten mice in each group.The first group was treated as control; the second group received standard therapeutics (diazepam, i.p.) and...
Article
Full-text available
Treatment monitoring is an essential aspect for tuberculosis (TB) disease management. Sputum smear microscopy is the only available tool for monitoring, but it suffers from demerits. Therefore, we sought to evaluate markers and cellular subsets of T regulatory (Treg) cells and T helper (Th) 17 cells in pulmonary TB patients (PTB) for TB treatment m...
Article
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Johne’s disease is world-wide in distribution and in the absence of control programs bio-load of Mycobacterium avium subspecies paratuberculosis continues to increase in domestic livestock and their milk in the country. For the diagnosis of MAP we developed novel recombinant secretary proteins based 'cocktail ELISA' by cloning using six highly immu...
Article
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2017) Mycobacterium avium subspecies paratuberculosis-an important food borne pathogen of high public health significance with special reference to India: an update, Veterinary Quarterly, 37:1, 282-299, ABSTRACT This review underlines the public health significance of 'Indian Bison Type' of Mycobacterium avium subspecies paratuberculosis (MAP) and...
Article
Aim: Maintenance of high-density microalgal culture forms the basic feature of algal biofuel technology.Algal biomass cultures explore few microalgal species over the other exposing these to grazers, pathogens and predators. So there is an urgent need to explore technologies that will permit early detection and control of these parasites. The prese...
Article
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This review underlines the public health significance of ‘Indian Bison Type’ of Mycobacterium avium subspecies paratuberculosis (MAP) and also its potential as ‘zoonotic infection’. In the absence of control programs, bio-load of MAP is increasing and if we take total population of animals (500 million plus) and human beings (1.23 billion plus) int...
Article
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Background: Type 2 diabetes (T2D) is a common multi-factorial disease that is primarily ac-counted to ineffective insulin action in lowering blood glucose level and later escalates to impaired insu-lin secretion by pancreatic β cells. Deregulation in insulin signaling to its target organs is attributed to this disease phenotype. Various genome-wid...
Data
Supplementary material is available on the publisher’s web site along with the published article.
Article
Increasing greenhouse gases, ozone depletion, and continued decline of fossil fuel reserves have necessitated researchers to search for a low input, high output, non-palatable fuel crop which should not compete with food crops for agricultural land and water. The only organisms that come close enough to possess all the above characteristic features...
Article
Full-text available
Mycobacterium avium subspecies paratuberculosis (MAP) is the cause of chronic incurable granulomatous enteritis in domestic livestock and has been associated with number of human autoimmune disorders like thyroiditis. Indigenous ELISA kit was used to monitor the sero-status of MAP infection in the patients of thyroiditis confirmed by pathology labo...
Article
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The present study was carried out to investigate the putative beneficial effects of insulin-like growth factor-I (IGF-I) and cysteamine supplementation alone or their combination on in vitro embryo development competence of fertilized goat oocytes. Presumptive zygotes (18 h post insemination) were randomly assigned for in vitro embryo development i...
Article
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The present study was aimed to assess the efficacy of different culture media for blastocyst development. Oocytes (2,539) from 1,059 ovaries recovered by follicle puncture technique were utilized for present investigation. In experiment 1, effect of different embryo culture media (Gr. 1; mKSOMaa; Gr. 2; TCM-199+OEC; Gr. 3; mSOFaa and Gr. 4; mCR2aa)...
Article
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Nowadays, the bioremediation of toxic pollutants is a subject of interest in terms of health issues and environmental cleaning. In the present review, an eco-friendly, cost-effective approach is discussed for the detoxification of environmental pollutants by the means of natural purifier, i.e., blue–green algae over the conventional methods. Indust...
Article
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Reports are few on the bio-incidence of Bovine Johne’s disease (BJD) in the important native breeds of buffaloes. Study estimated bio-incidence in the native tract of different buffalo breeds (Murrah, Zaffarabadi and Bhadawari) in dairy farms and farmer’s herds located in Central and North India. Of 156 serum samples screened from a dairy herd in K...
Article
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The present study compared the immune-reactivity of 3 antigens of Mycobacterium avium subspecies paratuberculosis (MAP) sourced from different geographical regions and livestock species for the diagnosis of Johne's disease (JD) in goats. Screening of 360 faecal and serum samples of goats by microscopy, i-ELISA, b-ELISA and r-ELISA gave 56.9, 40.0,...
Article
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Present study was carried out to test the efficacy of hot whole plant aqueous extract (HAE) of Solanum xanthocarpum Schrad and Wendl fam. Solanaceae against some gram positive and gram negative bacteria and its antioxidant activity. Hot whole plant aqueous extract (HAE) showed the significant(p>.01) antibacterial activity against the Bacillus subti...
Article
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Paratuberculosis (pTB) is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP) in a wide variety of domestic and wild animals. Control of pTB is difficult due to the lack of sensitive, efficacious and cost-effective diagnostics and marker vaccines. Microscopy, culture, and PCR have been used for the screenin...
Article
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The aim of this study was To characterize Mycobacterium tuberculosis isolates by MIRU-VNTR typing and IS 6110 – RFLP and to compare the efficiency of MIRU-VNTR typing and IS 6110 – RFLP in discriminating Mycobacterium tuberculosis isolates. In Mycobacterium genome a large number of insertional and repetitive elements have been identified. In the ge...
Article
Full-text available
Hot aqueous extract (HAE) of leaves of Anthocephalus cadamba (Roxb.) Miq, Rubiaceae has been screened for antimicrobial and antioxidant activity. Leaf extract was tested against some gram positive, gram negative and certain fungal strains. Leaf extract showed significant (p < .01) antibacterial and antifungal activity against Staphylococcus aureus,...
Article
Full-text available
The aim of this study was To characterize Mycobacterium tuberculosis isolates by MIRU-VNTR typing and IS 6110 – RFLP and to compare the efficiency of MIRU-VNTR typing and IS 6110 – RFLP in discriminating Mycobacterium tuberculosis isolates. In Mycobacterium genome a large number of insertional and repetitive elements have been identified. In the ge...
Article
Full-text available
Mycobacterium avium subspecies paratuberculosis (MAP), the cause of incurable Johne’s disease (JD), is endemic in both domestic livestock and human population of the country. It is the major cause of low per animal productivity and reduced productive life in domestic livestock, wherein buffaloes, goats and sheep go for early slaughter for harvestin...
Article
With the increase in environmental pollution in today's life, new techniques need to be evolved for its treatment too. In this regard we try to rely much on sustainable ways to treat the pollution. Bioremediation is one such promising technique in which microorganisms are used for the treatment of environmental pollutants and can be defined as biol...
Article
Full-text available
This work has been performed to study the efficacy of hot whole plant aqueous extract of Solanum xanthocarpum Schrad and Wendl on hematological and Biochemical parameters of wistar albino rats.Doses hot aqueous extract of different concentration like 125 mg/kg, 250 mg/kg, 500 mg/kg and 1000 mg/kg body weight was given orally to different group of r...
Article
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Plants play a major role on the earth and human beings depend on plants because of their medicinal properties. About 80% of the world population using plants as a medicinal drugs because plants have no side effects and show synergistic effect unlike modern medicine. In the present study, review and authentication of the various aspects of the plant...
Article
The Study aimed to compare the efficacy of MALDI-Tof identification system with conventional biochemical tests. Six water samples collected in duplex from water bodies of Mathura region were subjected to culturing, predominant strains isolation. Cultures were subjected to thirty-three different biochemical tests and MALDI-Tof-MS analysis. Biochemic...
Article
GM crops have been formed by altering the genetic makeup of traditional crop varieties. High profit margin, easy maintenance, potential to resist environmental changes has fascinated farmers to adapt these crops. In India more than 14 different research centers have been committed for the development of different GMO plants with Bt-Cotton, Bt-Brinj...
Article
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The present study aimed at comparing the relative efficacy of different capacitating agents used for sperm pretreatment during in vitro fertilization (IVF) and subsequent embryo development of caprine oocytes. In experiment 1, the effect of different concentrations of heparin (0, 20, and 50 μg/mL) on sperm pretreatment for different periods of time...
Article
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Human Papilloma Virus (HPV) infection is transmitted primarily via sexual and affects mostly basal epithelial cells of the skin or inner lining of tissues and are categorized as cutaneous types or mucosal types. It is known that HPV infection is strongly related to the development of cervical cancer, as viral DNA is detected in 99% of cervical canc...
Article
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Cross reactivity of three antigens of Mycobacterium avium subspecies paratuberculosis with sera of sheep endemic for Johne’s disease was evaluated. Out of 40 sheep tested by fecal microscopy, 72.5% were shedding MAP. Using protoplasmic antigens (PPA) from three MAP strains isolated from different livestock species and geographical regions, 90, 77.5...
Article
Full-text available
Johne's disease is endemic in the domestic riverine buffalo population of the country and bio-load of Mycobacterium avium subspecies paratuberculosis is increasing in the absence of indigenous diagnostic kits and control programs. A new 'dot-ELISA kit' has been developed and validated with indigenous plate ELISA for the screening of buffaloes again...
Article
The present study was aimed to evaluate the immunomodulatory activities of hot aqueous extract of pods & leaves (HAEP & HAEL) of C. fistula in albino rats. HAEP/HAEL were given oral dose of 125, 250 & 500 mg/ kg to healthy rats divided into one unfed (control group) and six fed groups. The humoral immune response was carried out by testing antibody...
Article
Full-text available
Objectives: The present study was undertaken to unravel the newer marker phytoconstituents in methanolic extract of Moringa oleifera leaves (MOLE) and evaluation of its immunomodulatory and splenocytes proliferation potential in rats. Materials and methods: Hot methanolic extract of MOLE was subjected to gas chromatography-mass spectrometry (GC-...
Article
Full-text available
Mycobacterium avium subspecies paratuberculosis (MAP), the cause of granulomatous chronic enteritis in ruminants (Johne’s disease) is under reported due to difficulties in diagnosing pre-clinical cases. Compromised specificity is a problem due to extensive sharing of antigens /epitopes among MAP and other mycobacterial strains. Culture filtrate (CF...
Article
Full-text available
In the quest to achieve success in fighting infections, innumerous antibiotics have been overexploited leaded to drug resistance, forcing researchers to focus on ayurvedic medicines. C. fistula, a native of Indian subcontinent is distributed throughout SouthEast Asia, is used as a medicinal plant for treatment of pruritus, leucoderma, diabetes, hae...
Article
Nothing shines like success. In this era of cutthroat competition in higher education, especially in life sciences, success requires not only hard work & determination but also key guidance. CSIR-NET-JRF is the stepping stone to build your career in life sciences. The paper provides an in-depth study about the pattern of examination, strategy invol...
Conference Paper
Fossil fuels are the sole energy source globally. Increased population size, industrial revolution have exploited these fuels, adding large amount of green house gases (CO 2 , CO, etc) into the atmosphere causing several environmental problems. In the attempt to quench our quest for fossil fuels several sources have been explored. First generation...
Conference Paper
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C. fistula a medicinal plant has been used in the treatment of various ailments in different parts of the world since ancient time. Studies indicated that the different parts of these plants contain a broad spectrum of secondary metabolites. The qualitative and quantitative analysis of hot aqueous extract of pods and leaves (HAEP & HAEL) was carrie...
Article
Full-text available
Bio-load and bio-profile of Mycobacterium avium subspecies paratuberculosis was studied in the domestic livestock population of the country. Of the 23 429 farm and farmer's animals screened, average bio-load was 23.3% (Period of study; 28 years for goats; 13 years for sheep, cattle and buffaloes). Species-wise, bio-load was 20.1, 32.7, 39.3 and 28....
Article
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The present study was undertaken to study the immuno modulatrory effects of leaves of Ocimum santum (OS) and Argemone mexiana (AM) plants in chicken model. 250 mg/kg body weight oral dose of OS and AM was found ideal and nontoxic in chickens and experimental chickens were fed this dose. Humoral immune response to S. enterica serovar Typhimurium 'O'...
Article
The present study was undertaken to explore the in vitro antiviral potential of hot aqueous extract (HAE) of Ocimum sanctum (OS) and Argemone mexicana (AM) leaves against Newcastle disease virus (NDV) and Infectious bursal disease virus(IBDV) in chicken embryo fibroblast (CEF) cell culture. First the nontoxic dose of HAE of OS and AM was determined...
Article
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In the present study 220 stool samples collected from diarrheic children admitted to different hospitals and nursing homes of Uttar Pradesh and Uttarakhand were screened for rotavirus. Of 220 diarrheic samples screened 46 samples were found to be positive for rotavirus by RNA PAGE. All the isolates exhibited 4-2-3-2 migration pattern suggesting gro...
Article
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Group-A rotaviruses are major causative agents of diarrhea in calves (under 1 month age) causing increased mortality in local cattle herds. 127 diarrheic fecal samples and 63 non diarrheic were collected from dairy farms of Mathura, India. 19 samples out of 127 diarrheic samples were found positive for rotaviruses by RNA-Polyacrylamide gel electrop...
Chapter
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Algae like some other higher plants, produces stored lipids in the form of triacylglycerol (TAG). Comparatively algae produce more oil than any other biofuel technology which is currently being used. Micro-algal species can accumulate substantial quantity of lipid, sometimes even 50% of their biomass. Algae can inhabitant a wide variety of natural...
Conference Paper
Full-text available
Algae like some other higher plants, produces stored lipids in the form of triacylglycerol (TAG). Comparatively algae produce more oil than any other biofuel technology which is currently being used. Micro-algal species can accumulate substantial quantity of lipid, sometimes even 50% of their biomass. Algae can inhabitant a wide variety of natural...
Article
Haemorrhagic septicaemia (HS) caused by Pasteurella multocida (P. multocida) B:2 is a fatal disease of cattle and buffaloes causing heavy mortality in untreated animal within 72 hours. Oil adjuvanted vaccines are in use in many countries, but associated with local adverse reactions and short-term immunity. Present study attempts to improve the HS v...
Article
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Neonatal diarrhoea is one of the most important conditions of calves, associated with morbidity and mortalities. Diarrhoeal diseases have an adverse effect on calf health status, survival and productive performances. Corona virus is one of the etiological agents responsible for calf diarrhea worldwide. However there is paucity of literature stating...