Asha Dilrukshi Wijegunawardana

Asha Dilrukshi Wijegunawardana
Rajarata University of Sri Lanka · Department of Bioprocess Technology

B.Sc. in Agri (Biotechnology spp), M.Phil (Molecular Biology, PhD (Molecular Biology)

About

60
Publications
11,851
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
176
Citations
Introduction
My research interest included molecular biology research work related to both plant and animals. Currently, I am doing research related to the use of sterile insect technique for the control of Aedes mosquitoes in Sri Lanka. I have skills and experience for most of the molecular biology technique from DNA extraction, PCR, RT-PCR, tissue culture methods, gene transformation techniques, microarray techniques, etc.
Additional affiliations
January 2020 - March 2020
Rajarata University of Sri Lanka
Position
  • Lecturer
Description
  • Undergraduate teaching - Genetics & Evaluation, Molecular Biology
April 2014 - January 2020
University of Kelaniya
Position
  • Research Assistant
Description
  • Assisting & supervising rooting mosquito surveillance, establishment & maintaining of the insectary, laboratory practices, preparation & maintaining the lab report & SOPs, an arrangement of lab meetings, purchasing of lab chemicals & equipment.
February 2011 - March 2014
Anti Filariasis Campaign, Colombo, Sri Lanka
Position
  • Research Officer
Education
July 2014 - December 2017
University of Kelaniya
Field of study
  • PhD degree in Molecular Biology
May 2008 - September 2010
University of Kelaniya
Field of study
  • MPhil degree in Molecular Biology
January 2001 - July 2006
Wayamba University of Sri lanka
Field of study
  • B.Sc. Honours degree in Agriculture majoring Biotechnology

Publications

Publications (60)
Article
Full-text available
The objective of this study was to develop a site directed geographic information system (GIS) map of lymphatic filariasis (LF) in Gampaha District, Sri Lanka as a guide for targeted control activities. Epidemiological and entomological screening of LF was carried out in nine pre-identified endemic areas in Gampaha District, using night blood scree...
Article
Full-text available
To compare Wuchereria bancrofti (W. bancrofti) infection rates of Culex quinquefasciatus, using dissection and PCR-ELISA in two consecutive time periods (from 2007 to 2008 and from 2008 to 2009). Mosquitoes were collected in 30 sentinel and 15 non-sentinel sites in 15 Medical Officer of Health areas of Gampaha District known for the presence of W....
Article
Full-text available
Background: Mass Drug Administration (MDA)-based Global Lymphatic filariasis (Lf) eradication programmes are aimed at stopping transmission of Wuchereria bancrofti by its mosquito vector. The study was designed to compare one year post treatment (mass distribution of Diethylcarbamazine-Albendazole) infection rates of Wuchereria bancrofti in Culex q...
Article
Full-text available
Background: The Sri Lankan Anti-Filariasis Campaign conducted 5 rounds of mass drug administration (MDA) with diethycarbamazine plus albendazole between 2002 and 2006. We now report results of a comprehensive surveillance program that assessed the lymphatic filariasis (LF) situation in Sri Lanka 6 years after cessation of MDA. Methodology and pri...
Article
Full-text available
Background: Many low-income and middle-income country (LMIC) researchers have disadvantages when applying for research grants. Crowdfunding may help LMIC researchers to fund their research. Crowdfunding organizes large groups of people to make small contributions to support a research study. This manuscript synthesizes global qualitative evidence a...
Presentation
No: 221) With the onset of the COVID-19 pandemic, the use of medicinal plants for their immune-enhancing properties is becoming popular in human society, since they can be used safely and inexpensively, instead of pharmaceuticals with uncertain side effects and the higher cost.
Presentation
Full-text available
The Motion Graphic video, as featured on TDR’s YouTube channel https://youtu.be/4BQ-DAILR2E
Conference Paper
Full-text available
Mosquitoes transmit serious human diseases causing millions of deaths every year. The use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational costs. Insecticides of plant origin have been reported as useful for the control of mosquitoes. Therefo...
Conference Paper
Full-text available
Abstract With the onset of the COVID-19 pandemic, the use of medicinal plants for their immune-enhancing properties is becoming popular in human society. They can be used as safe and inexpensive alternatives for pharmaceuticals with uncertain side effects and the higher cost. However, evaluating and standardization of these herbal immune booster fo...
Cover Page
Full-text available
Editorial Article: ‘I love to read users' reviews before making a purchase decision’ Senior lecturer Asha Wijengunawardana reveals her passion for engaging with the community on vector-borne infectious diseases and emphasizes the importance of lab product reviews
Technical Report
Full-text available
Overview Public engagement is increasingly recognized as an important part of science. Robust community involvement can spur innovation, meaningfully connect with local communities and improve health outcomes. Recognizing this change, many major science funders now require public engagement in research studies. How can we organize more effective pu...
Conference Paper
Full-text available
Dengue is one of the most important arboviral diseases in humans and a rapidly spreading mosquito-borne viral ailment in the world. The primary vector of the dengue virus is the Aedes aegypti mosquito. Synthetic insecticides are widely used to repress mosquitoes. However, it instigates problems such as insecticide resistance among insects, bio-accu...
Presentation
Abstract presentation of the Peradeniya University International Research Sessions (iPURSE) 2021, Sri Lanka, on 11th November 2021 (Abstract No. iPURSE21ABS00178).
Preprint
Full-text available
Background: Many exceptional researchers, especially in low-and middle-income countries (LMICs) face structural disadvantages in applying for research grants. Public engagement and crowdfunding make it possible for researchers to directly seek support from the public, by engaging large groups of people who make small contributions to support a proj...
Article
Full-text available
Dengue virus is a serious public health issue in tropical and subtropical regions. The global incidence of dengue necessitates potent antiviral medication for the prevention of proliferation of the virus inside the human body. The DEN2 NS2B/NS3 protease presents in the dengue virus, is an attractive drug target due to its essential role in viral re...
Conference Paper
Full-text available
Dengue virus is a serious public health issue in tropical and subtropical regions. The global incidence of dengue necessitates the potent antiviral medication for the prevention of proliferation of the virus inside the human body. The DEN2 NS2B/NS3 protease presents in the dengue virus, is an attractive drug target due to its essential role in vira...
Article
Full-text available
Phlebotomus argentipes is the main suspected vector for leishmaniasis in Sri Lanka. Investigations on the presence of aerobic bacteria in the gut of sand flies which evidence a potential approach to control leishmaniasis transmission through a paratransgenic strategy are still not available for the local sand fly populations. Field-caught unfed fem...
Article
Full-text available
The establishment of a laboratory colony is an essential first step for conduction of laboratory studies on the biology and control of Aedes albopictus mosquitoes. Therefore, with the objective of generating high quality mosquitoes as research material for utilization in ongoing vector control studies and to disseminate authenticated, high-quality...
Article
Aedes aegypti L. (Diptera: Culicidae), being the primary vector of pathogenic arboviruses, is a target for the development of novel genetic approaches to complement current conventional vector control strategies such as the combined sterile insect and incompatible insect technique (SIT/IIT). A transinfected line of Ae. aegypti carrying the wAlbB Wo...
Article
Full-text available
Background Sex separation of mosquitoes at different stages is currently being attempted to ensure the successful release of male mosquitoes in novel vector control approaches. Mechanical and behavioral techniques have been tried most frequently. Methods Batches of (n = 300) Aedes aegypti and Ae. albopictus pupae were used for standard sieving (us...
Article
Full-text available
Constant monitoring of Aedes vector indices such as Aedes mosquito abundance and ovitrap data is important for the control of dengue epidemics. Therefore, the current study attempted to evaluate the effect of larval and climatic factors on the incidence of dengue outbreaks in the Gampaha district. Based on the distribution of previously reported de...
Conference Paper
Full-text available
Introduction: This study focused on the effectiveness of using a novel and environmental friendly strategy of combined sterile insect technique (SIT) and a Wolbachia-based incompatible insect technique (IIT) approach in Aedes albopictus population control efforts. The above strategy uses the intracellular endosymbiont bacteria Wolbachia, in insects...
Conference Paper
Full-text available
This study addresses the possibility of using a novel and environmental friendly strategy of combined sterile insect technique (SIT) and a Wolbachia-based approach in Aedes albopictus population control efforts in Sri Lanka. The above strategy combines irradiation and the intracellular endosymbiont bacteria Wolbachia, in insects which are verticall...
Presentation
Full-text available
Abstract Presentation conducted at the 19th Conference on Postgraduate Research International Postgraduate Research Conference- 2018 Presentation title: "The potential use of a combined sterile insect technique (SIT) and Wolbachia-based approach for the control of the dengue vector �Aedes albopictus in Sri Lanka."
Article
Lymphatic filariasis (LF) is the second most common vector-borne disease globally. Approximately 90% of the estimated 120 million LF infections are caused by Wuchereria bancrofti. Routinely, W. bancrofti infection is diagnosed by morphological identification of microfilariae by microscopy, which is labour intensive, less sensitive and time consumin...
Article
Investigations on oviposition behaviour of dengue vectors are critical for effective controlling of vector breeding. Therefore, the aim of the present study was to determine the oviposition behaviour of dengue vectors, Aedes aegypti and Aedes albopictus in Sri Lanka. Batches of 1000 adult mosquitoes (1 : 1, male: female ratio) housed in rearing cag...
Poster
Full-text available
INTRODUCTION In the absence of a 100% reliable sex separation method Sterile Insect Technique (SIT) combined with Insect Incompatible Technique (IIT) is being considered as a promising strategy as it appears to be one of the safest and sustainable methods towards population control of mosquito vector pests. SIT is based on the mass rearing of a tar...
Conference Paper
Full-text available
The current research study focused on the possibility of using a novel and environmental friendly strategy of Wolbachia bio-pesticide in dengue vector control as an additional tool in the integrated dengue vector control in Sri Lanka. The above method uses Wolbachia, an obligate intracellular bacterium that lives inside insects and is transmitted v...
Presentation
Full-text available
Abstract presentation at the Peradeniya University International Research Sessions (iPURSE) - 2016
Conference Paper
Full-text available
Dengue fever is a rapidly emerging arthropod-borne viral disease that has threatened approximately one-third of the world‟s population. Due to the absence of an effective drug or vaccine for dengue, prime focus of health sectors lies on the biological control of vector densities via innovative approaches such as Sterile Insect Techniques and Incomp...
Conference Paper
Full-text available
symbionts like Wolbachia can be useful tools for the control of major agricultural pests and disease vectors, including Aedes mosquitoes. The objective of the present study was to determine and characterize the presence of Wolbachia infections in wild mosquito populations in Sri Lanka in order to identify suitable strains which may be useful in the...
Conference Paper
Full-text available
Most of the innovative vector mosquito control methods such as Sterile Insect Techniques (SIT) and Incompatible Insect Techniques (IIT) require mass rearing and releasing of sterile male mosquitoes in large scale. Thus, effective sex separation methods are needed to eliminate female mosquitoes from the mass releases, ensuring that the potential tra...
Conference Paper
Full-text available
Three months ovitrap survey was conducted to assess the suitable position in placing the ovitraps for dengue vector mosquito surveillance and this study was initiated due to loss of valuable data from our previous studies as a result of physical damage of the ground kept ovitarps. Thirty four households in the Ragama Medical Officer of Health area...
Conference Paper
Full-text available
There is an increasing demand for exploration of the potential for applying the Sterile Insect Technique (SIT) in area-wide integrated vector management (AW-IVM) in many countries. However, because female mosquitoes, unlike male mosquitoes, can transmit disease, means to eliminate them from the mass production process are a critical prerequisite. I...
Research
Full-text available
Abstract presented at the International Research Sessions in University of Peradeniya, Sri Lanka (6th November, 2015).
Cover Page
Full-text available
SA Comprehensive Assessment of Lymphatic Filariasis in Sri Lanka Six Years after Cessation of Mass Drug Administration CITED 43 TIMES as of July 2020.
Thesis
Full-text available
Entomological and epidemiological tools are considered important to monitor the chemotherapy-based filariasis eradication programs aimed at stopping the transmission of Wuchereria bancrofti by its obligatory mosquito vector. Objectives of this study were the comparison of dissection of mosquitoes and Polymerase Chain Reaction Enzyme-Linked Immunoso...
Poster
Full-text available
Background: The ecology, development, behavior, and survival of mosquitoes and the transmission dynamics of the diseases they transmit are strongly influenced by climatic factors. Objective: The objective of this study was to identify the population density variation of Culex mosquitoes within a period of one year in the Gampaha district, Sri Lanka...
Conference Paper
Full-text available
Some methods of mosquito population control, especially the application of pesticides for adult mosquitoes, can have an impact on the environment and individuals with a heightened sensitivity to pesticides. Of the available control tools, biological controls are considered to be generally host-specific and environmental friendly. Although biologica...
Conference Paper
Full-text available
Entomological surveys are important tools for monitoring progress of lymphatic filariasis (Lf) eradication programs. In this study, dissection of Culex quinquefasciatus was compared with a Polymerase Chain Reaction - Enzyme Linked Immunosorbent Assay (PCR-ELISA) for pooled mosquitoes to assess filarial infection levels in the major vector of Wucher...
Conference Paper
Full-text available
Introduction: Lymphatic filariasis (Lf) is the second most common vector-borne disease globally. Approximately 90% of global burden of Lf is caused by Wuchereria bancrofti. W. bancrofti is routinely diagnosed by morphological identification of microfilariae (Mf) by microscopy which is a labour intense, low sensitive and time consuming method. Detec...
Conference Paper
Full-text available
Human infection with Wuchereria bancrofti causes a disabling parasitic disease known as lymphatic filariasis, which is a major public health and socio-economic problem in many parts of the world. Little is known about the prevalence of filariasis among high-risk populations for filariasis. Objective of this study was to determine such prevalence of...
Conference Paper
Full-text available
Lymphatic Filariasis (LF) is the leading cause of lymphoedema worldwide, affecting an estimated 120 million people. Gampaha, which is outside the filarial belt in Sri Lanka, has been found to have higher endemicity of filariasis and was included in the National Filariasis Control Program. Entomological surveys are critical for evaluation of filaria...

Questions

Questions (2)
Question
Can anyone suggest highest efficient PCR cloning kit for ligation strand of less than 400 bp use for diagnostic purposes?
Question
I am interesting to learn some species identification keys in order Diptera and Lepidoptera. if you have any references or books which I could use for this purposes please let me know.
Thanks. 

Network

Cited By