Arzu Tezvergil

Arzu Tezvergil
University of Turku | UTU · Department of Cariology and Restorative Dentistry

DDS, PhD,

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167
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Publications

Publications (167)
Article
Objective: To determine whether smear layer management, via conservative etching protocols, and the hydrophobic-rich content of hybrid layers would affect the fatigue strength of resin-dentin interfaces. Methods: Bar-shaped dentin beams obtained from sound third molars were wet-polished for 30 s. Dentin was etched with 32 % ortho-phosphoric acid...
Article
Objectives To determine whether the composition of universal adhesives and the use of silane coupling agents could affect the fatigue strength of composite repair. Methods Composite samples were aged in water at 37 °C for 90 days and bonded to fresh composite to produce twin-bonded bar-shaped composite specimens (2 × 2 × 12 mm). Five universal adh...
Article
Endogenous dentin proteases contribute to the degradation of collagen fibrils in the hybrid layer. Recently, inhibition of host‐derived proteases by curcuminoids has shown promising results. The aim of this study was to evaluate the effect of curcuminoid treatment on the microtensile bond strength (μTBS) after 24 h or 12 months of storage. Fifty‐fo...
Presentation
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Clinical evaluation of dimethyl sulfoxide pretreatments on the durability of composite restorations
Article
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Objective To determine whether the effect of dentin moisture on the etch-and-rinse bonding may be minimized by dry-bonding protocols utilizing aqueous or ethanolic dimethyl sulfoxide (DMSO) pretreatments. Methods H3PO4-etched mid-coronal dentin surfaces from human molars were randomly blot- or air-dried for 30 s and pretreated with DMSO/H2O or DMS...
Article
Full-text available
Objective To characterize whether the bonding performance and fatigue strength of resin-dentin interfaces created by a universal adhesive would be affected by different H3PO4-application times to more accurately assess long-term durability. Methods Mid-coronal flat dentin surfaces with standardized smear-layers were produced on sound third molars,...
Article
Objective Imperfect polymer formation as well as collagen’s susceptibility to enzymatic degradation increase the vulnerability of hybrid layers over time. This study investigated the effect of new dimethyl sulfoxide (DMSO)-containing pretreatments on long-term bond strength, hybrid layer quality, monomer conversion and collagen structure. Methods...
Article
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We evaluated the effect of phytic acid on matrix metalloproteinase (MMP)‐ or cysteine cathepsin (CC)‐mediated dentin degradation. Demineralized dentin beams were divided into five groups (n = 12) and treated with 1%, 2%, or 3% phytic acid or with 37% phosphoric acid. Untreated demineralized beams served as controls. After incubation for 1 or 3 wk,...
Article
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This study evaluated the cytotoxicity of methacrylate‐based resins containing dimethyl sulfoxide (DMSO). DMSO was incorporated into hydrophobic (R2) and hydrophilic (R5) resins at weight concentrations of 0, 0.01, 0.1, 1, 5, or 10 w/w %. Resin discs (n = 10/group) were prepared. Human gingival fibroblasts (HGF‐1) were exposed to resin eluates for 2...
Article
The study evaluated changes in total enzymatic activity and degradation of demineralized dentin following the application of universal or self-etch adhesives. The universal adhesives -Scotchbond Universal (SU) and All-Bond Universal (ABU) and self-etch adhesives -Adper Easy Bond (EB) and G-aenial Bond (GB) were used for 2 min pretreatment of the de...
Article
Selective carious tissue-removal strategies require specific considerations in selection of restorative materials. A tight marginal seal placed over hard dentin and sound enamel is essential. For selective removal of carious tissue with permanent restoration, bioactive materials, such as high-viscosity glass-ionomer cement (HV-GIC) or calcium silic...
Article
Full-text available
In dentistry, the wet-bonding approach relies on water to maintain demineralized collagen expanded for proper resin infiltration; nevertheless, hydrolytic instability of the resin-dentin interface is inevitable with current bonding techniques. Considering dimethyl sulfoxide's (DMSO) ability to "biomodify" collagen and precipitate enzymes, the aim w...
Article
Matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs) can break down unprotected type I collagen fibrils in dentin matrix. This study investigated the use of potassium fluoride (KF) as a potential inhibitor of MMPs and CCs in dentin. Demineralized dentin beams were divided into groups (n = 10 in each group) and incubated in artificial sali...
Article
Objectives Dentin biomodification using collagen cross-linkers has been proposed as one of the strategies to improve bond durability of adhesives to dentin. However, literature is not very consistent regarding their benefit, in particular when cross-linkers are applied in clinically realistic application times. This study investigated the effect of...
Article
Objectives: Matrix metalloproteinases (MMPs) are dentinal endogenous enzymes claimed to have a vital role in dentin organic matrix breakdown. The aim of the study was to investigate presence, localization and distribution of MMP-7 in sound human dentin. Methods: Dentin was powdered, demineralized and dissolved in isoelectric focusing buffer. Res...
Article
Objective: Matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs) are two distinct enzymatic pathways responsible for the degradation of collagen fibrils in demineralized dentin. NaF and KF have been shown to inhibit salivary MMP-2, -9 and CCs. This study investigated the inhibitory effect of calcium fluoride (CaF2) on the dentin matrix-bo...
Article
Objectives: To evaluate whether selective dentin etching protocols using reduced phosphoric acid (H3PO4) etching-times would affect the resin-dentin interaction of a universal adhesive to improve long-term bonding effectiveness. Methods: Mid-coronal flat dentin surfaces were produced on sound third molars, selectively etched with 32% H3PO4 for 3...
Article
Purpose Fluoride compounds have been reported to play a protective role in the host-derived enzymatic degradation of demineralized dentin matrices. The objective of this study was to evaluate the effect of fluoride pretreatment on the immediate and long-term microtensile bond strength (µTBS) of resin-dentin interfaces. Materials and methods Mid-co...
Article
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Objective: To determine whether bonding effectiveness and hybrid layer integrity on acid-etched dehydrated dentin would be comparable to the conventional wet-bonding technique through new dentin biomodification approaches using dimethyl sulfoxide (DMSO). Methods: Etched dentin surfaces from extracted sound molars were randomly bonded in wet or d...
Article
The aim of this study was to evaluate the effect of curcuminoids on the dentin endogenous protease activity. Demineralized dentin were pretreated with 50 or 100 µM of three different curcuminoids for 60 s and incubated up to 3 months. Untreated beams served as controls. Dry dentin mass was measured after incubation. Aliquots were analyzed for the q...
Article
Full-text available
Objective: To investigate the repair bond strength of fresh and aged nanohybrid and hybrid composite resins using a universal adhesive (UA). Materials and methods: Fresh and aged substrates were prepared using two nanohybrid (Venus Pearl, Heraus Kulzer; Filtek Supreme XTE, 3 M ESPE) and one hybrid (Z100, 3 M ESPE) composite resin, and randomly assi...
Chapter
With the introduction of adhesive technologies, minimally invasive, tooth-colored, adhesive restorations have replaced conventional restorations that sacrificed sound tooth structure for extra retention. Fiber-reinforced composite restorations have gained wide interest due to the possibility of using directly at the chair-side replacement of missin...
Article
Objective: The objectives of the study were to evaluate the ability of a 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide (EDC)-containing primer to improve immediate bond strength of either self-etch or etch-and-rinse adhesive systems and to stabilize the adhesive interfaces over time. A further objective was to investigate the effect of EDC on the...
Article
The enzymatic degradation of dentin organic matrix occurs via both the action of matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs). Zinc can prevent collagen hydrolysis by MMPs. However, its effect on the activity of dentin-bound CCs is not known. The aim of this study was to investigate the effect of zinc on matrix-bound cathepsin K a...
Conference Paper
Objectives: The objective of this study was to evaluate the microtensile bond strength (µTBS) and nanoleakage (NL) of bonded resin-dentin interfaces pretreated with various concentrations of dimethyl sulfoxide (DMSO). Methods: Extracted sound human third molars were used in this study. Occlusal enamel was removed under water cooling and teeth were...
Article
Objective: To compare the effect of a cross-linker pretreatment on the fatigue crack growth resistance of resin-dentin bonds prepared with a two- vs. three-step adhesive system. Methods: Bonded interface Compact Tension (CT) specimens were prepared using commercial two- and three-step etch-and-rinse adhesives and compatible hybrid resin-composit...
Article
A water-soluble crosslinking agent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), has been used as a pretreatment of acid-etched dentin to inactivate matrix-bound endogenous dentin proteases. The aim of this study was to evaluate the effect of pH on the inactivation capacity of EDC. Demineralized dentin beams (1 × 2 × 6 mm) were divided int...
Article
Introduction Cigarette smoking is one of the major risk factors in the pathogenesis of periodontal disease. Despite numerous reports on the general toxic effects of oral smokeless tobacco very few studies have assessed the effect of smokeless tobacco on the viability and inflammatory response of gingival epithelial cells. Material and Methods In t...
Article
The aim of this study was to evaluate the degradation of completely demineralized dentin specimens in contact with a filler-free or 2 ion-releasing resins containing micrometer-sized particles of Bioglass 45S5 (BAG) or fluoride-containing phosphate-rich bioactive glass (BAG-F). Fourier transform infrared spectroscopy (FTIR) and scanning electron mi...
Article
Objective: To examine the influence of a cross-linking treatment using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) on hybrid layer stiffness of resin-dentin adhesive bonds, using spatially-resolved nanoscopic Dynamic Mechanical Analysis (nanoDMA). Methods: Bonded interface specimens were prepared using a two-step (SB) or three-step (SBM...
Article
This study investigated the antimicrobial efficacy and mechanical strength of hard and soft denture liners modified with benzalkonium chloride (BAC). The specimens (1 mm thickness, 8 mm diameter) were prepared by mixing 0.5, 1, 2 and 5 wt% BAC with soft (Sofreliner Medium, Tokuyama) and hard (Rebase II, Tokuyama) denture liners (n = 5/group). BAC w...
Conference Paper
Purpose/Aim: The purpose of this study was to determine the elastic modulus ofdemineralized dentin beams immersed in various concentrations of dimethyl sulfoxide (DMSO) or ethanol for different time intervals. Materials and methods: Dentin beams (1mm×2mm×6mm) were completely demineralized in 10% phos- phoric acid. After baseline measurements of ela...
Article
Objective: Inactivation of dentin proteases by crosslinkers has been suggested as a way to prevent the degradation of dentin collagen in the hybrid layer. However, it is not known if the inhibition is reversible. The aim of this study was to evaluate the inactivation effect of various crosslinkers on dentin protease activity over a period of 6 mon...
Article
Full-text available
Objective: This study evaluated a new approach, named dimethyl sulfoxide (DMSO)-wet bonding, to produce more desirable long-term prospects for the ultrafine interactions between synthetic polymeric biomaterials and the inherently hydrated dentin substrate. Methods: Sound third molars were randomly restored with/without DMSO pretreatment using a...
Article
Purpose: This paper evaluates the changes in surface properties and softness of soft relining materials after cyclic loading in water. Materials and methods: 3 polysiloxane (Silagum AM Comfort, Molloplast B, Mollosil Plus) and 2 acrylic-based (Vertex Soft, Astron LC Soft) proprietary soft relining materials and a vinyl polysiloxane (Imprint 2 Ga...
Article
Matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs) degrade the collagen fibrils of demineralized dentin. Sodium fluoride (NaF) has previously been shown to inhibit recombinant MMP-2 and MMP-9. This study aimed to evaluate the efficacy of NaF on the inhibition of dentin-bound MMPs and CCs. Dentin beams were completely demineralized in 10...
Conference Paper
Objective: The objective of this study was to evaluate water sorption and solubility of one hydrophilic and one hydrophobic experimental resin containing different concentrations of DMSO. Methods: Two experimental methacrylate- based resins of known hydrophilicities were used, one hydrophobic resin (R2) composed of Bis-GMA, TEGDMA, EDMAB. The seco...
Article
Full-text available
The use of tooth-colored dental restorative materials, along with the adhesive techniques, has become routine in today’s dental practice. The longevity of adhesive restorations depends mainly on good bonding between restorative materials and tooth structure, which should be achieved in situ, within minutes. While bonding to enamel is reliable throu...
Article
Objective: To characterize the effect of an EDC pretreatment on the fatigue crack growth behavior of resin-dentin bonds. Methods: Bonded interface Compact Tension (CT) specimens were prepared using a three-step etch-and-rinse adhesive and hybrid resin-composite. Adhesive bonding of the treated groups included a 1min application of an experimenta...
Conference Paper
Objective: The objective of this study was to evaluate the effect of different concentrations of dimethyl sulfoxide (DMSO) pretreatment on permeability of demineralized dentin, determined by amount of HEMA uptake in the demineralized dentin. Methods: Dentin cubes (2x2x2mm) were prepared from coronal portion of noncarious third molars. The cubes wer...
Article
The aim of this study was to evaluate the effect of using UVA-induced crosslinking with or without riboflavin as photosensitizers on degradation of dentin matrix by dentin proteases. Demineralized dentin specimens (0.4×3×6mm(3), n=10/group) were subjected to: (RP1), 0.1% riboflavin-5 phosphate/UVA for 1min; (RP5), 0.1% riboflavin-5 phosphate/UVA fo...
Article
This study evaluated the effect of dentin pretreatment with collagen crosslinkers on matrix metalloproteinase (MMP) and cathepsin K mediated collagen degradation. Dentin beams (1mm×2mm×6mm) were demineralized in 10% H3PO4 for 24h. After baseline measurements of dry mass, beams were divided into 11 groups (n=10/group) and, were pretreated for 5min w...
Article
This study tested whether treatment of demineralized dentin with polyacrylic acid (PAA) has any activatory or inhibitory activity on dentin matrix metalloproteinases (MMP)s or cathepsin K (CAT-K). Dentin beams (1mm×2mm×6mm; n=10) were completely demineralized with EDTA. After initial dry mass assessment, the beams were dipped into 37% phosphoric ac...
Article
This work measured the amount of bound versus unbound water in completely-demineralized dentin. Dentin beams prepared from extracted human teeth were completely demineralized, rinsed and dried to constant mass. They were rehydrated in 41% relative humidity (RH), while gravimetrically measuring their mass increase until the first plateau was reached...
Article
Objectives: This study evaluated the endogenous matrix metalloproteinase (MMP) activity of demineralized dentin matrix following 1 or 5min pretreatment by various collagen crosslinkers. Generic MMP activity assay, total protein analysis, in situ zymography, gelatin zymography and multiplex bead technology were used to evaluate matrix-bound MMP act...
Article
Full-text available
The aim of this study was to evaluate the microleakage of direct restorative composite resin upon the addition of an intermediate glass fiber-reinforced composite (GFRC) layer of various fiber orientations between tooth and particulate filler composite resin (PFRC). Cavities were prepared both on the mesial and distal surfaces of sixty extracted hu...
Article
Dentin matrices release ICTP and CTX fragments during collagen degradation. ICTP fragments are known to be produced by MMPs. CTX fragments are thought to come from cathepsin K activity. The purpose of this study was to determine if quaternary methacrylates (QAMs) can inhibit matrix MMPs and cathepsins.Methods Dentin beams were demineralizated, and...
Conference Paper
Objective: The degradation of resin-dentin bonds are, in part, attributed to the endogenous protease activity in dentin. Both matrix metalloproteinase (MMP) and cysteine cathepsin (CC)-mediated degradation was shown in demineralized dentin matrices. Sodium fluoride (NaF) was previously shown to inhibit (MMP) -2 and -9. The aim of this study was to...
Conference Paper
Objective: Cysteine cathepsines (CC) and matrix metalloproteinases (MMP) have been shown to be responsible for the degradation of resin-dentin bonds. The aim of this study was to evaluate the immediate microtensile bond strength (µTBS) of resin-dentin bonds after pretreatment with specificMMP- or cathepsin-inhibitors (Galardin or E-64, respectively...
Conference Paper
Objective: Dentin biomodification with collagen crosslinkers has been shown to reduce the endogenous enzymatic degradation at the resin-dentin interface. The aim of this study was to evaluate the effect of six collagen crosslinkers on the immediate and one-year micro push-out (µPO) bond strength to dentin. Method: Eighty-eight molars were div...
Article
Full-text available
This study analyzed the amounts of solubilized telopeptides cross-linked carboxyterminal telopeptide of type I collagen (ICTP) and C-terminal crosslinked telopeptide of type I collagen (CTX) derived from matrix-metalloproteinases (MMPs) and cysteine cathepsins (CTPs) subsequent to application of a filler-free (Res.A) or an ion-releasing resin (Res....
Conference Paper
Full-text available
Objectives: The poor durability of resin-dentin bonds created by contemporary adhesives is thought to be due, in part, to the enzymatic degradation of collagen by matrix metalloproteinases (MMP)s or cathepsine-K (CAT-K) in the hybrid layer. Biomodification of dentin by collagen crosslinkers should inactivate all dentin proteases simultaneously. The...
Article
Full-text available
Objective: The clinical survival rates of the adhesive restorations are limited due to the deterioration of resin-dentin bonds over time, partly due to the endogenous enzymatic activity of dentin. Recently, benzalkonium chloride (BAC) has been shown to effectively inhibit endogenous protease activity of dentin. This study evaluated the effect of d...
Conference Paper
Objective: The aim of this study was to evaluate the fatigue resistance of anterior two-unit chair-side cantilever bridges reinforced with unidirectional fiber-reinforcements. Method: Ninety extracted upper canines were used (n=15 per group). Preparation designs were: lingual and buccal supporting wing (F1), 2 mm approximal box with lingual wing (F...
Conference Paper
The XRD studies of Ramachandran et al. (1968), Belli et al., (1994-1996) and Kramer et al., (1999-2000) and NMR studies of Fullerton et al. (2006) and Cameron et al. (2007) demonstrated that most proteins are surrounded by bound water, especially collagen. Objective: As little work has been done on bound water in dentin, the purpose of this work wa...
Conference Paper
Objective: Matrix metalloproteinases (MMPs) and cathepsins were both identified in human dentin and in part responsible for the collagen degradation in hybrid layers created by dentin adhesives. The aim of this study was to evaluate the effect of phosphoric acid (PA) concentration and demineralization duration on the protease activity in dentin. M...
Article
A methylmethacrylate-free denture base polymer (Eclipse) in comparison to a conventional denture base polymer (Palapress vario) was evaluated after water saturation and Stick glass fibre reinforcement. The data were analysed with ANOVA at a = 0.05. Water-storage caused a decrease in the flexural strength and stiffness of the materials (p > 0.05). C...
Article
Full-text available
The purpose of this study was to analyze the shear bond strength of a new composite resin to polymer-based composite substrates using various surface roughnesses and two kinds of polymer matrices. Particulate filler composite resin with cross-linked polymer matrix and fiber-reinforced composite with semi-interpenetrating polymer matrix were used as...
Conference Paper
Objective: Activation of matrix metalloproteinases (MMPs) and cathepsins during the acid-etching step of the restorations are partly responsible for the collagen degradation in hybrid layers created by dentin adhesives. The objective of this study was to determine the effect of dental laser (DL) irradiation on the metalloproteinases-mediated dentin...
Conference Paper
Objective: Endogenous protease activity of dentin compromised the integrity of hybrid layers. Previously, the use of collagen crosslinkers was shown to inhibit matrix metalloproteinases (MMPs). The aim of this study was to evaluate the effect of various collagen crosslinkers on MMP-2 and -9 activity of demineralized dentin. Method: Powdered denti...