Arvind Kumar ShuklaPusan National University | PNU · Department of Biomedical Convergence Engineering
Arvind Kumar Shukla
M.Sc. Genetic Engineering Ph.D Biomedical Convergence Engineering
Biofabrication of Functional Tissue/Organs by 3D Bioprinting, Organ-on-a-chip (OOC), Stem cells & Cancer
About
38
Publications
16,686
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Introduction
Arvind Kumar Shukla is working on the Molecular mechanism of Cancer Stem Cells Biology. He is the main focus in the fabrication of functional tissue/organs development by 3D Bioprinting with a study on Stem cells & Cancer signaling pathways and also focuses on various cancer drugs delivery, which are used tested clinically for treatment. Email ID: arvindkumarshuklapnu@gmail.com
Additional affiliations
January 2012 - October 2014
D Y Patil University Mumbai
Position
- Master's Research Student
Description
- Genetic Engineering
Education
February 2020 - February 2024
Field of study
- Biomedical Convergence Engineering (3D Bioprinting, Organ-on-a-chip (OOC), Stem Cells, Cancer).
July 2012 - July 2014
DY Patil University
Field of study
- Genetic Engineering (HIV, Stem Cells, Cancer)
February 2008 - July 2012
Publications
Publications (38)
Sphingosine kinases (Sphk1 and 2) regulate the prodution of sphingosine-1-phosphate (S1P), that is key molecule in cancer development. SphK1, which is commonly overexpressed in malignant tumours, significantly contributes to the pathogenesis of various types of cancer as well as to resistance to different Tyrosine Kinase inibitors (TKIs). Even, Sph...
![Correlation between Lung Cancer Prognosis and COVID-19 infection [141]](profile/Arvind-Kumar-Shukla/publication/386582272/figure/tbl1/AS:11431281296013663@1733814108434/Correlation-between-Lung-Cancer-Prognosis-and-COVID-19-infection-141_Q320.jpg)
the COVID-19 pandemic might hamper. COVID-19-related lung cancer patients’ incidental radiologic abnormalities might show up in regular radiology exams, making it challenging to understand disease progression and the effect of therapy. The COVID-19 pandemic has profoundly impacted lung cancer care, highlighting challenges in patient management, tre...
The ongoing COVID-19 pandemic underscores the urgent need for effective vaccination strategies to mitigate disease burden. The development of neutralizing antibodies is a critical indicator of host defence mechanisms against life-threatening infectious diseases, such as those caused by SARS-CoV-2. Understanding the fundamental biological mechanisms...
Comparative Analysis of Knowledge, Attitude, and Practices (KAP) for Innovation Management Processes to Enhance Healthcare Delivery in Hospital Administration
Page. 24
ABSTRACT
This Knowledge, Attitudes, and Practices (KAP) survey explores the landscape of
innovation management processes within hospital management contexts. Healthcare
innovation is pivotal for addressing evolving patient needs, optimizing operational
efficiency, and advancing clinical outcomes. This study aims to understand the
perceptions, atti...
The Knowledge, Attitudes, and Practices (KAP) survey, a widely used research tool, explores
innovation management in hospital settings, highlighting its significance in addressing patient needs, enhancing
operational efficiency, and improving clinical outcomes. The quantitative method of the KAP survey measures a
target population's knowledge, atti...
Cancer vasculogenesis is a pivotal focus of cancer research and treatment given its critical role in tumor development, metastasis, and the formation of vasculogenic microenvironments. Traditional approaches to investigating cancer vasculogenesis face significant challenges in accurately modeling intricate microenvironments. Recent advancements in...
At the end of 2019, a new disease was reported in Wuhan China and described such as severe acute respiratory syndrome (SARS). This new virus named SARSCoV-2 S is characterized by high infectivity among humans. Such disease can have no symptoms in some individuals, while in others can provoke flu like symptoms or acute respiratory distress syndrome,...
The skin is composed of many cells that are organized into different layers and connected by dense and complex vascular networks. This creates a dynamic microenvironment in which cells interact within the matrix. Significant advancements have been made in this field over the past decade, and various strategies have been developed for accelerating a...
Those affected by cancer or receiving cancer treatment have often been more susceptible to infections due to coexisting chronic diseases, overall poor health status, and systemic immunosuppressive states caused by both cancer and anticancer treatments. This pioneering text is an introduction to the key topics in the relationship between infection,...
High-quality academic publishing is built on rigorous peer review. Bioengineering was able to uphold its high standards for published papers due to the outstanding efforts of our reviewers. Thanks to the efforts of our reviewers in 2022, the median time to first decision was 15 days and the median time to publication was 40 days. Regardless of whet...
High-quality academic publishing is built on rigorous peer review. Diagnostics was able to uphold its high standards for published papers due to the outstanding efforts of our reviewers. Thanks to the efforts of our reviewers in 2022, the median time to first decision was 18 days and the median time to publication was 38 days. Regardless of whether...
High-quality academic publishing is built on rigorous peer review. Advances in Respiratory Medicine was able to uphold its high standards for published papers due to the outstanding efforts of our reviewers. Thanks to the efforts of our reviewers in 2022, the median time to first decision was 23 days and the median time to publication was 64 days....
Induced pluripotent stem cells (iPSCs) are essentially produced by the genetic reprogramming of adult cells. Moreover, iPSC technology prevents the genetic manipulation of embryos. Hence, with the ensured element of safety, they rarely cause ethical concerns when utilized in tissue engineering. Several cumulative outcomes have demonstrated the func...
GLOBOCAN 2020 estimated more than 19.3 million new cases, and about 10 million patients were deceased from cancer in 2020. Clinical manifestations showed that several growth factor receptors consisting of transmembrane and cytoplasmic tyrosine kinase (TK) domains play a vital role in cancer progression. Receptor tyrosine kinases (RTKs) are crucial...
At the end of 2019, a new disease recognized such as severe acute respiratory syndrome (SARS), was reported in Wuhan, China. This disease was caused by an unknown SARS coronavirus 2 (SARS-CoV-2); a virus is characterized by high infectivity among humans. In some cases, this disease can be asymptomatic, while in other cases can induce flu-like sympt...
The effectiveness of cancer treatment strongly depends on the early detection of the disease. Currently, the most common diagnostic method, tissue biopsy, takes time and can be damaging to the patient. Circulating cancer biomarkers such as circulating tumor DNA, micro-RNA (miRNA), tumor proteins, exosomes, and circulating tumor cells have repeatedl...
Today, there are major environmental challenges associated with hazardous
waste management in India. In this regard, hazardous production is increasing rapidly
and it's required proper management to be improving the future of India. In this
context, the Indian government's policy and legislation are not working properly on
Hazardous Waste Managemen...
The Government of India is taking all necessary steps to ensure that to prepared well to face the challenge and threat posed by the growing pandemic of COVID-19 the Corona Virus. With active support of the people of India, we have been able to contain the spread of the Virus in our country. The most important factor in preventing the spread of the...
Cancer is multiple genetic and epigenetic events. The cancer development and progression, tumor cells undergo abnormal epigenetic modifications, including DNA methylation, histone deacetylation and nucleosome remodeling.Currently many scientists and laboratory focus for a new method for cancer treatment and prevention such as genome editing(CRISPR-...
LabRoots for the 5th Annual Genetics and Genomics free virtual conference. Genetics and Genomics 2017 will cover the most recent and exciting advances in genetics research.
Chronic myeloid leukemia (CML) originates from pluripotent hematopoietic stem cells that acquire translocation between the BCR gene on chromosome 22 and the ABL proto-oncogene on chromosome 9. Such rearrangement leads to the the Philadelphia (Ph) chromosome and the oncogenic fusion protein formation. This oncoprotein induces cell proliferation, cau...
Many study shows dietary habits as a modifier of many cancer risk and tumor behavior. Nutrigenomics represents a strategy that can be applied to the study and prevention of many cancer type. It correlated with a molecular relationships between nutrients and genes (nutrigenetics), and these interactions influence changes in the profile of transcript...
Many study shows dietary habits as a modifier of many cancer risk and tumor behavior.Nutrigenomics represents a strategy that can be applied to the study and prevention of many cancer type. It correlated with a molecular relationships between nutrients and genes (nutrigenetics), and these interactions influence changes in the profile of transcripts...
In our previous study, we have shown that SphK1 is up-regulated in imatinib-resistant CML cells by a pathway contingent on
a phosphoinositide 3-kinase/AKT2/mammalian target of rapamycin signaling pathway. In order to better understand this
mechanism, we examined the signaling pathways responsible for transcriptional up-regulation of SphK1in imatini...
The current trend of study molecular genetic studies have revealed some critical mutations underlying the pathogenesis of the sporadic and inherited forms of colorectal cancer (CRC). A relatively limited number of oncogenes and tumor-suppressor genes-most prominently the APC, KRAS, and p53 genes-are mutated in a sizeable fraction of CRCs, and a lar...
Regorafenib, an oral small-molecule small-molecule multi kinase inhibitor, is able to block Vascular Endothelial Growth Factor Receptors (VEGFR-1, 2 and 3), Platelet-Derived Growth Factor Receptors (PDGF), Fibroblast Growth Factor (FGF) receptor 1, Raf, TIE-2, and the kinases KIT, RET, and BRAF. Different studies have displayed its antitumor activi...
Background: The development of assay which is fast targeting bacterial mRNA detection which provide status of mycobacterial infection. mycobacterial viability is detected amplification tests directed at DNA or rRNA targets, mRNA is more rapidly destroyed in cells than rRNA or genomic DNA. This study was carried out to optimize reverse transcriptase...
Colorectal cancer is among four most common malignancies and the second leading cause of cancer death in the western world. Epidermal Growth Factor Receptor (EGFR) and Vascular Endothelial Growth Factor (VEGF) are often overexpressed in colorectal cancer and are associated with inferior outcomes. More recently, further improvements in survival have...
The current trend of burn wound care has shifted to a more holistic approach of improvement in the long-term form and function of the healed burn wounds and quality of life. This has demanded the emergence of various skin substitutes in the management of acute burn injury as well as post burn reconstructions. Skin substitutes have important roles i...
Background: Abacavir is an antiretroviral indicated in combination with other antiretroviral agents for the treatment of HIV-1 infection. It is associated with potentially serious hypersensitivity reactions in 3–9% of Caucasian patients. Carriers of HLA-B*5701 are at high risk for developing abacavir hypersensitivity reaction (AHR). In Caucasians,...
Sirtuins are highly conserved all the way from bacteria to humans. These proteins play important roles inside the cell, as nicotinamide adenine dinucleotide-dependent protein deacetylases. In addition, they are major regulators of multiple cellular functions since they can modify the epigenetic landscape and are able to alter the proteome of the ce...
Abacavir (ABC) is a nucleoside analogue, a reverse transcriptase inhibitor, used for combination antiretroviral therapy in HIV-infected patients. It has been extensively evaluated in clinical studies and is currently recommended as a preferred or an alternative nucleoside reverse transcriptase inhibitor in antiretroviral-naive patients according to...
Questions
Question (1)
Flow cytometry is an effective tool for detecting apoptosis, helping researchers distinguish between live cells, apoptotic cells, and necrotic cells. This guide describes how to use flow cytometry with Annexin V and propidium iodide (PI) staining to detect apoptosis.
1. Sample preparation
Collect cells from culture and wash with cold PBS to remove any residual culture medium. Resuspend cells in binding buffer (provided in most apoptosis detection kits) to maintain viability during staining. For best results, perform apoptosis detection soon after treatment or exposure to stressors, as cells may go through apoptotic stages.
2. Annexin V and propidium iodide staining
Annexin V conjugated to a fluorophore (such as FITC or APC) is added to detect phosphatidylserine on the outer membrane of cells, which is exposed early in apoptosis. Propidium iodide (PI) is added to distinguish between late apoptotic and necrotic cells. PI only enters cells with damaged membranes, staining DNA in late apoptotic or necrotic cells. Incubate cells with both stains for 15 minutes at room temperature in the dark, as light exposure may degrade the fluorophores.
3. Flow cytometry settings
Analyze samples on a flow cytometer, using filters appropriate for Annexin V and PI. Typically, a dual laser setting (488 nm for PI and 633 nm for Annexin V-APC) works well. Make sure forward and side scatter settings are optimized to cover all cell sizes and types.
4. Data analysis
Analyze cells using flow cytometry software, dividing them into four quadrants:
Annexin V-/PI-: live cells
Annexin V+/PI-: early apoptotic cells
Annexin V+/PI+: late apoptotic or secondary necrotic cells
Annexin V-/PI+: necrotic cells
This allows for clear distinction between apoptotic stages.
Flow cytometry provides a quantitative, reliable method for detecting apoptosis, providing valuable insights into cellular responses to treatment or stress.
Reference
[1] Lanzhou Jiang et al, Scientific Reports 2017 (https://lnkd.in/evKCDxZX)
