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Arumugam Kumaresan

Arumugam Kumaresan
Southern Regional Station of ICAR - National Dairy Research Institute · Theriogenology

M.V.Sc., Ph D (Veterinary Gynecology and obstetrics)

About

278
Publications
88,217
Reads
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2,689
Citations
Additional affiliations
January 2017 - present
Southern Regional Station of ICAR-National Dairy Research Institute
Position
  • Principal Investigator
April 2010 - March 2011
Sveriges Lantbruksuniversitet
June 2009 - January 2017
National Dairy Research Institute
Position
  • Senior Researcher

Publications

Publications (278)
Article
Estrus detection is a major problem in buffaloes because of the poor expression of estrus signs leading to low reproductive efficiency. Salivary transcripts analysis is a promising tool to identify biomarkers; therefore, the present study was carried out to evaluate their potential as estrus biomarkers. The levels of HSD17B1, INHBA, HSPA1A, TES tra...
Article
Anti‐Müllerian hormone (AMH) is a member of the TGF‐β superfamily produced by follicular granulosa cells (GCs) in women and cattle and is considered an endocrine biomarker of ovarian follicular reserve. The study examined how age and parity influence serum AMH concentration and investigated the presence of Single Nucleotide Polymorphisms in AMH gen...
Article
Full-text available
This study aimed to estimate the changes in the milk yield, milk fat, energy indicators [NEFA, BHBA, Dry Matter Intake (DMI) and Body Condition Score (BCS)] and concentration of innate immune molecules (Haptoglobin: Hp, Serum Amyloid A: SAA, TLR-4, TNF-α, IL-1β, IL-6, and IL-8), during the transition period in primiparous and multiparous dual-purpo...
Article
Full-text available
Protamines are the nuclear proteins essential for chromatin compaction during spermatogenesis. During chromatin compaction, histones are replaced by transition proteins, which are then replaced by protamines. This process is essential for DNA stability. Protamines are rapidly evolved proteins with high evolutionary variation and encompass positivel...
Chapter
Infrared thermography (IRT) is a non-invasive technique that measures mid- to long-wave infrared radiation emitted from all objects and converts this to temperature. The thermogram depicts visual representation of the energy emitted, transmitted, and reflected by an object and its environment. IRT is a simple, effective, on-site, and non-invasive m...
Article
Full-text available
Bull fertility is of paramount importance in bovine industry because semen from a single bull is used to breed several thousands of cows; however, so far, no reliable test is available for bull fertility prediction. In the present study, spermatozoa from high- and low-fertility bulls were subjected to high-throughput transcriptomic, proteomic and m...
Article
Bull fertility is of paramount importance in bovine industry because semen from a single bull is usedto breed several thousands of cows; however, so far, no reliable test is available for bull fertility prediction. In the present study, spermatozoa from high- and low-fertility bulls were subjected to high-throughput transcriptomic, proteomic and me...
Book
This book provides updated information on the current concepts in bovine reproduction. It describes the complex issues associated with fertility and infertility in bovines and suggests strategies for achieving high reproductive efficiency. It discusses topics related to the fertility trend in bovines, estrus detection, controlled breeding, postpart...
Preprint
The expression of genes related to lactogenesis in peripheral blood mononuclear cells (PBMCs) are less understood. To understand the biology of milk production in early lactating non pregnant cows in the blood, the transcriptome from PBMCs was analysed by RNA sequencing. We are able to generate a total of ~ 62.5 Gbp RNA-Seq data files (~ 24 million...
Article
The reason for poor semen quality among the breeding bulls is not well understood. In the present study, we performed high throughput RNAseq analysis of spermatozoa to identify the SNPs present in good and poor quality semen producing Holstein Friesian breeding bulls. A total of 21360 and 44650 SNPs were identified in good and poor quality semen wi...
Chapter
The first-generation reproductive technology, artificial insemination is the tool that expedited the genetic progress in cattle and buffaloes during last few decades. The genetic improvement can be achieved only when superior quality bulls are used for artificial insemination. Even when the superior bulls are used for cryopreservation, their sperma...
Research
Full-text available
Accurate determination of estrus is essentially required for efficient reproduction management of farm animals. Buffalo is a shy breeder and does not manifest overt signs of estrus that make estrus detection difficult resulting in a poor conception rate. Therefore, identifying estrus biomarkers in easily accessible biofluid such as saliva is of utm...
Article
Seminal plasma proteins and pathways associated with sperm motility have not been elucidated in stallions. Therefore, in the current study, using the high throughput LC/MS-MS approach, we profiled stallion seminal plasma proteins and identified the proteins and pathways associated with sperm motility. Seminal plasma from six stallions producing sem...
Preprint
Mastitis is the top most reason for antibiotic usage, which is an important public health concern due to antimicrobial resistance development in pathogen. Citrate is an important precursor for milk synthesis and regulation of homeostasis between Ca ²⁺ and H ⁺ ions and thereby milk fluidity. The aim of this study was to evaluate the effect of trisod...
Article
The aim of the present study was to identify fertility associated sperm membrane proteins in crossbred bulls. Sperm membrane proteins from high‐ and low‐fertile Holstein Friesian crossbred bulls (n = 3 each) were subjected to high‐throughput liquid chromatography–mass spectrometry (LC–MS/MS) for comparative proteomic analysis. Proteomic profiling i...
Conference Paper
Full-text available
Estrus detection is a major problem in buffaloes because of poor expression of estrus signs and higher incidences of silent estrus especially during summer season. Salivary transcript analysis is a noninvasive and promising tool for biomarker discovery. Hence, present study was carried out to evaluate the potential of salivary transcripts as estrus...
Conference Paper
Estrus detection is a major problem in buffaloes because of poor expression of estrus signs and higher incidences of silent estrus especially during summer season. Salivary transcript analysis is a noninvasive and promising tool for biomarker discovery. Hence, present study was carried out to evaluate the potential of salivary transcripts as estrus...
Preprint
Background : Estrus detection is a major problem in buffaloes because of the poor expression of estrus signs leading to low reproductive efficiency. Salivary transcripts analysis is a promising tool to identify biomarker; therefore, the present study was carried out to evaluate their potential as estrus biomarker. Methods : The levels of HSD17B1 ,...
Article
Full-text available
Spermatozoa carries a reservoir of mRNAs regulating sperm functions and fertilizing potential. Although it is well recognized that a considerable proportion of high genetic merit breeding bulls produce poor-quality semen, the transcriptomic alterations in spermatozoa from such bulls are not understood. In the present study, comparative high-through...
Article
Full-text available
Antimicrobials use (AMU) is the key driver for development of antimicrobials resistant (AMR) pathogen in human and veterinary medicines. Therefore, understanding AMU pattern is prerequisite for focused intervention on AMR. The aim of this study was to understand the AMU pattern and their indications in dairy farm and individual farmer production co...
Article
Full-text available
Male fertility is extremely important in dairy animals because semen from a single bull is used to inseminate several thousand females. Asthenozoospermia (reduced sperm motility) and oligozoospermia (reduced sperm concentration) are the two important reasons cited for idiopathic infertility in crossbred bulls; however, the etiology remains elusive....
Chapter
The success of artificial insemination and optimal use of genetically superior bulls is determined by the fertility of the bull, which in turn depends on sperm quality in frozen semen. Therefore, precise estimation of sperm fertilizing potential is important for achieving greater fertility in dairy bovines. There were the days when semen quality wa...
Chapter
Spermatogonial stem cells (SSCs) are the only adult stem cells in males capable of transmitting their genetic information to the next generation. Over the past two decades, spermatogonial stem cell transplantation has shown colossal potential for fertility restoration and transgenesis in livestock. It is based on the transfer of SSCs from fertile d...
Article
Full-text available
The present study was conducted to evaluate the functional membrane integrity of cryopreserved spermatozoa from stallions belonging to two contrasting motility groups (high and low motility groups). A dual staining method comprising of carboxy fluoroscein diacetate (CFDA) and propidium iodide (PI) was used for this purpose. Stallions were classifie...
Article
Full-text available
Prolonged oestrus is one of the conditions associated with perturbed follicular dynamics that culminates into substantial economic losses to dairy industries through low success rate of artificial insemination, increased inter-calving period, reducing total milk yield, lowering calf production, shortening reproductive life of the animals, lowering...
Article
The incidence of sub‐fertility is higher in crossbred bulls compared to zebu bulls. In the present study, we analysed the metabolomic profile of seminal plasma from crossbred and zebu bulls and uncovered differentially expressed metabolites between these two breeds. Using a high‐throughput LC‐MS/MS‐based approach, we identified 990 and 1,002 metabo...
Article
Full-text available
In this study, we evaluated the peripheral concentrations of Toll-like receptors (TLR)-4 during transition period in relation to postpartum productive and reproductive performances of Deoni (zebu) cattle. Accuracy and threshold values of TLR-4 to predict the postpartum performance were also estimated using receiver operating characteristics (ROC) a...
Article
During transit and storage in epididymis, spermatozoa undergo final maturation, ac- quire motility, functional competence and the ability to fertilise an oocyte. Epididymal secretions contain a complex biochemical milieu of diverse inorganic ions, proteins, metabolites and other molecules. Since it is believed that spermatozoa are translationally...
Article
Full-text available
Crossbreeding of indigenous cattle (Bos indicus) with improved (Bos taurus) breeds gained momentum and economic relevance in several countries to increase milk production. While production performance of the crossbred offspring is high due to hybrid vigor, they suffer from a high incidence of reproductive problems. Specifically, the crossbred males...
Article
Full-text available
Background Low conception rate (CR) despite insemination with morphologically normal spermatozoa is a common reproductive restraint that limits buffalo productivity. This accounts for a significant loss to the farmers and the dairy industry, especially in agriculture-based economies. The immune-related proteins on the sperm surface are known to reg...
Article
In the present study, we standardized an in vitro oviduct explants model for cattle and assessed the oviduct explants binding ability and phenotypic characteristics of spermatozoa obtained from breeding bulls with high- and low-sperm DNA fragmentation index (DFI%). Cryopreserved spermatozoa from Holstein Friesian crossbred breeding bulls (n=45) wit...
Article
The present study was carried out to compare the proteomic profiles of spermatogenic cells of crossbred and zebu cattle in an effort to understand the possible reasons for a higher incidence of sub-fertility in crossbred bulls. The spermatogenic cells collected from the testes of pre-pubertal (6 mo) and adult (24 mo) crossbred and zebu males throug...
Article
Full-text available
Crossbred bulls produced by crossing Bos taurus and Bos indicus suffer with high incidence of infertility/subfertility problems; however, the etiology remains poorly understood. The uncertain predictability and the inability of semen evaluation techniques to maintain constant correlation with fertility demand for alternate methods for bull fertilit...
Article
Background: The selection of genetically superior animals at an early stage of life, the molecular markers are used along with traditional selection. The study was carried out to identify the genetic polymorphism in the exon3 region of the Prolactin and enumerate its effect on milk production performance in Sahiwal cattle. Prolactin plays an impera...
Article
Background: Improper estrus detection causes huge economic losses due to inaccurate time of insemination leading to poor conception rate. So, here pedometer proved to be a promising tool in defining optimal time of insemination,improving fertilization rates based on increased number of steps. The study is to determine the relationship between incre...
Article
Poor semen quality and infertility/subfertility are more frequent in crossbred than zebu bulls. Using a high-throughput liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based approach, we established the preliminary metabolomic profile of crossbred and zebu bull spermatozoa (n=3 bulls each) and identified changes in sperm metabolomics betw...
Article
Full-text available
Receiver Operating Characteristics (ROC) analysis is a popular method to discriminate the two conditions of tested animals. In this study, we estimated accuracy and threshold values of metabolic (Dry matter Intake; DMI and Body Condition Score: BCS, NEFA and BHBA) and immune indicators (Haptoglobin: Hp, Serum Amyloid A: SAA, IL-6, TNF-α, IL-1β, and...
Article
The presence of various forms of RNAs having roles in fertilisation and early embryonic development is well documented in mammalian spermatozoa. In the present study, using Agilent microarray platform, we compared sperm mRNA expression profiles between high‐ and low‐fertile crossbred bulls with normal semen parameters. Microarray data acquisition a...
Article
Full-text available
The milk and milk products from cows reared under grazing system are believed to be healthier and hence have high demand compared to milk from cows reared in the non-grazing system. However, the effect of grazing on milk metabolites, specifically lipids has not been fully understood. In this study, we used acetonitrile precipitation and methanol:ch...
Article
Ethnopharmacological relevance A combination of Trachyspermum ammi L., Curcuma longa L., Cuminum cyminum L., Trigonella foenum-graecum L., Foeniculum vulgare Mill., Anethum graveolens L and Zingiber officinale Roscoe is used as immunity booster and reproductive efficiency enhancing agents in folklore medicine. Aim of the study The present study ai...
Article
Genetic modification of spermatogonial stem cells followed by their transplantation to homologous recipients has shown immense potential to complement the current methods being used for generating transge-nic farm animals. Using this technique, the time duration required to generate transgenic founder animals can be reduced at least by half, which...
Article
Full-text available
Although, it is known that spermatozoa harbor a variety of RNAs that may influence embryonic development, little is understood about sperm transcriptomic differences in relation to fertility, especially in buffaloes. In the present study, we compared the differences in sperm functional attributes and transcriptomic profile between high- and low-fer...
Preprint
Full-text available
Background Low conception rate (CR) despite insemination with morphologically normal spermatozoa is a common reproductive restraint which limits buffalo productivity. This accounts for a significant loss to the farmers and the dairy industry, especially in agriculture-based economies. The immune-related proteins on the sperm surface are known to re...
Article
The objective of this study was to assess the influence of spermatozoa surface antigenic proteins on the functional competence of bovine neat and frozen-thawed semen. The breeding bulls (n = 38) were screened for seminal antigenic levels in neat semen based on the agglutination titrations with anti-sperm antibody (ASA). Bulls having high (n = 8) an...
Article
Full-text available
Understanding the changes in the spermatozoa during cryopreservation is indispensable for tailoring and increasing the efficiency of cryopreservation process success. However, the dynamics of damage to sperm organelles during different stages of cryopreservation is underexplored. This study assessed the mitochondrial membrane potential (MMP) and DN...
Article
Full-text available
Background: The testicular biometry such as scrotal circumference (SC) and scrotal volume, affect semen quality hence bull fertility and thus testicular biometry can be used for breeding soundness of bulls. Methods: The present study was carried out to study the testicular parameters of 77 Sahiwal males of different ages and to establish its import...
Article
Full-text available
Sperm, which are believed to be transcriptionally and translationally inactive, synthesize RNA and proteins before there is gradual disappearance of the ribosome during chromatin compaction. Sperm transfer several functionally relevant transcripts to the oocyte, controlling maternal-zygotic transition and embryonic development. The present study wa...
Article
Although reduced reproductive efficiency during summer has been well documented in buffaloes, the reason for the same is yet to be understood. The present study was conducted to identify the subtle differences in sperm phenotypic characteristics (motility, membrane integrity, acrosome reaction and lipid peroxidation status), oviduct binding ability...
Article
Full-text available
Objective: To assess the stability of 10 candidate internal control genes (ICGs), namely GAPDH, ACTB, RPL23, RPS15A, ATPSF1, GLUT5, HMBS, ATP2B4, PPIA, and BRP to normalize the transcriptional data from testes samples of Zebu and crossbred bulls. Methods: Total RNA was isolated from testicular tissue of Zebu and crossbred bulls (n=6 each) between 2...
Article
Spermatogonial stem cell transplantation provides a unique opportunity to study the biology of spermatogenesis and also offers an alternative approach for genetic modification in large animals. The present study aimed to extend this technique to the water buffalo. Spermatogonial stem cells (SSCs) were isolated from prepubertal buffalo testes (3–6 m...
Article
Full-text available
The glycans on the plasma membrane of cells manifest as the glycocalyx, which serves as an information-rich frontier that is directly in contact with its immediate milieu. The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific function...
Article
Full-text available
The aim of this study was to estimate the prevalence of subclinical mastitis (SCM), its innate immunity and to compare the efficacy of indirect diagnostic tests [California mastitis test (CMT), differential electrical conductivity (DEC), absolute EC, enzyme based color detection method (Tanucheck kit) and brothymol blue strip (BTB) tests] to milk s...
Article
Full-text available
Background: The incidence of poor semen quality and sub-fertility/infertility is higher in crossbred as compared to Zebu males. Several attempts have been made to understand the possible reasons for higher incidence of fertility problems in crossbred males, at sperm phenotype, proteome and genome level but with variable results. Since the quality...
Article
Full-text available
Retention of fetal membranes (RFM) in bovines not only lowers fertility and productivity but also leads to significant economic loss to the dairy farms. The present study compared peripartum progesterone, estradiol, cortisol, Prostaglandin F2 metabolite (PGFM) and relaxin concentrations in crossbred cows with normal parturition (NP) and with RFM. B...