Arthur Fernandes Siqueira

Arthur Fernandes Siqueira
Tohoku University | Tohokudai · Department of Environmental Life Sciences

Life Sciences Ph.D.

About

13
Publications
23,379
Reads
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175
Citations
Additional affiliations
September 2013 - September 2014
July 2011 - present
Brazilian Agricultural Research Corporation (EMBRAPA)
Position
  • Sctructural and functional genomics of diazotrophs allowed for commercial inoculants production in Brazil
Description
  • Studies related to plant-diazotrophs interaction using genomic approaches.
Education
February 2011 - March 2013
Londrina State University
Field of study
  • Genomics and Microbiology

Publications

Publications (13)
Article
Full-text available
N2O is an important greenhouse gas influencing global warming, and agricultural land is the predominant (anthropogenic) source of N2O emissions. Here, we report the high N2O-reducing activity of Bradyrhizobium ottawaense, suggesting the potential for efficiently mitigating N2O emission from agricultural lands. Among the 15 B. ottawaense isolates ex...
Preprint
Full-text available
N 2 O is the major greenhouse gases influencing global warming, and agricultural land is the predominant (anthropogenic) source of N 2 O emissions. Here, we report the high N 2 O-reducing activity of Bradyrhizobium ottawaense , suggesting the potential for efficiently mitigating N 2 O emission from agricultural lands. Among the 15 B. ottawaense iso...
Article
Full-text available
Soybean plants host endosymbiotic dinitrogen (N2)-fixing bacteria from the genus Bradyrhizobium. Under oxygen-limiting conditions, Bradyrhizobium diazoefficiens and Bradyrhizobium japonicum perform denitrification by sequentially reducing nitrate (NO3–) to nitrous oxide (N2O) or N2. The anaerobic reduction of NO3– to N2O was previously shown to be...
Article
Full-text available
The soybean symbiont Bradyrhizobium diazoefficiens grows anaerobically in the presence of nitrate using the denitrification pathway, which involves the nap, nir, nor, and nos genes. We previously showed that NasT acts as a transcription antitermination regulator for nap and nos gene expression. In the present study, we investigated the targets of N...
Article
Full-text available
When soil oxygen levels decrease, some bradyrhizobia use denitrification as an alternative form of respiration. Bradyrhizobium diazoefficiens (nos⁺) completely denitrifies nitrate (NO3–) to dinitrogen, whereas B. japonicum (nos–) is unable to reduce nitrous oxide to dinitrogen. We found that anaerobic growth with NO3– as the electron acceptor was s...
Article
Full-text available
Background The soybean-Bradyrhizobium symbiosis can be highly efficient in fixing nitrogen, but few genomic sequences of elite inoculant strains are available. Here we contribute with information on the genomes of two commercial strains that are broadly applied to soybean crops in the tropics. B. japonicum CPAC 15 (=SEMIA 5079) is outstanding in it...
Article
Full-text available
The 450-million-year-old interaction between the majority of land plants and mycorrhizal fungi is one of the most ancient, abundant, and ecologically important symbiosis on earth. The early events in the evolution of mycorrhizal symbioses seem to have involved reciprocal genetic changes in ancestral plants and free-living fungi. new data on the mec...
Article
Full-text available
Utilização de enzimas do estresse oxidativo como biomarcadoras de impactos ambientais Using oxidative stress enzymes as biomarkers in environmental impacts Resumo O uso intensivo de compostos xenobióticos em atividades humanas é responsável pela contaminação de vários ambientes, provocando alterações em diferentes níveis ecológicos. O monitoramento...
Article
Full-text available
Desvendando a H + -Pirofosfatase vacuolar e o seu papel na biotecnologia vegetal Unravelling the vacuolar H + -Pyrophosphatase and its role on plant biotechnology Resumo As proteínas de membrana são de fundamental importância para manter o funcionamento da célula e a homeostase iônica dos seres-vivos. Há uma diversidade de proteínas que ajudam a ma...
Article
Full-text available
s) autor(es). Publicado pela ESFA. Queiroz S, Maximiniano A, Siqueira AF, Cruz ZMA & Ramos AC (2007) Parâmetros físico-químicos e microbiológicos do processo de atenuação natural monitorada em área contaminada por solventes clorados. Natureza on line 5(1): 10-18. [on line] http:
Article
Full-text available
Resumo O uso intensivo de compostos xenobióticos em atividades humanas é responsável pela contaminação de vários ambientes, provocando alterações em diferentes níveis ecológicos. O monitoramento das possíveis consequências do uso indiscriminado desses compostos é realizado por intermédio de biomarcadores ou bioindicadores, principalmente em ambient...

Questions

Questions (20)
Question
I had a personal communication about it. I was told that, sometimes, when the promoter is TOO strong, the desired gene target can be not over-expressed or can be inhibited.
I searched for some bibliography but I could not find it.
Thank you very much.
Question
Inside the Computer Science and Biology fields.
Question
Crop Yield: Harvested per unit of land area, normally measured in metric tons per hectare (or kilograms per hectare).
Question
nif genes have an important role in the nitrogen fixation process. In the nifRLA operation, what is the role of the nifR gene, and which proteins are encoded?
Question
For example: Genes responsible to nitrogen fixation in Rhizobia, genes responsible for photosynthesis in A. thaliana etc. They are classified as critical genes, right?
Question
I want to perform a simple comparison test between 3 genomes, build a Venn diagram to analyze the percentage of common, exclusive and different genes among these 3 genomes.
Question
I have found it in a bacteria genome during an analysis in slilico. Thanks, Merry Christmas and Happy New Year.
Question
I have my metagenome uploaded in MG-Rast. How can I download the data and use "order" (level) in STAMP to run statistical analysis?
Question
If I have a hypothetical protein in my genome sequence (bacterial genome sequence) and I want to do a lab study (not silico analysis) to validate this protein, what are the basics steps to goal? (For example an ABC transporter).

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