Arthur Martynov

SI "I. Mechnikov Institute of microbiology and immunology of NAMSU" · Molecular immunopharmacology

Introduction The paper presents the results of a study on the first synthesized benzimidazole derivatives obtained from labile nature carboxylic acids. The synthesis conditions of these substances were studied, their structure was proved, and some components were found to have sugar-reducing activity on the model of alloxan diabetes in rats. Metho...

Combining probiotic bacteria is a promising strategy to increase the effectiveness and avoid side effects of probiotic therapy. Bacteria that find themselves in a common environment are able to both change their properties and show new ones under the influence of each other. The change of colonization and antagonistic properties, which provide bact...

Enzymatic reactions are the basis of many biotechnological manufacturing and biomedical diagnostic procedures that require effective methods of monitoring over the reaction course. In the current paper, we present the results of the development of a new approach within the differential microwave dielectrometry method for the non-invasive monitoring...

Objectives: Polymyxin is the last line of defense against resistant forms of microorganisms, but it has significant nephrotoxicity. One of the directions in reducing the nephrotoxicity of polymyxin is to modify the charge of the molecule and accordingly, to change the topicity of the polymyxin derivative to the renal megalin. Such modification can...

Nisin is a typical representative of class I bacteriocins belonging to subtype A1 lantibiotics. This lantibiotic is produced by gram-positive bacteria of the genera Lactococcus, Streptococcus, Staphylococcus, and Blautia. Currently, at least twelve natural variants of nisin are known. They differ in some physicochemical properties and biological ac...

Introduction. Fountains (office and outdoor), being small decorative structures used to improve the parameters of the human life environment, in the process of their work during the generation of a water jet, determine the creation of spray and aerosol fractions, thereby allowing the loss of a certain volume of liquid when air moves and wind carryo...

Nisin is the most well-known representative of subtype A1 lantibiotics. This small (< 5 kDa) peptide ribosomally produced by Gram-positive bacteria belonging to Lactococcus, Streptococcus, Staphylococcus and Blautia species. Nisin exhibits antibacterial activity against a broad range of Gram-positive bacteria and may be effective against Gram-negat...

Objectives The study aimed to investigate the structural aspects of polymyxins nephrotoxicity at the atomic level to promote the more purposeful development of the polymyxin’s derivatives with the lower nephrotoxic action. Materials and methods The molecular dynamics simulations of the complexes of polymyxin B and its derivative NAB7061 (that carr...

Subject and Purpose. This study focuses on the original waveguide-differential dielectrometer designed for complex permittivity measurements of high-loss liquids in the microwave range towards the determination of pharmaceutical ingredient concentrations in water solutions at room temperature. The suitability of the device and effectiveness of the...

Aristolochia clematitis has long ago been applied in Asian traditional medicine and demonstrates multidirectional pharmacological activity. Contemporary research has confirmed its expressed antimicrobial activity. Using high performance liquid chromatography (HPLC) we ascertained and determined the content of aristolochic acid 1 in herb and roots o...

The aim of the research was to evaluate the antimicrobial potential of cell-free extracts obtained in various ways from the probiotic strain Lactobacillus reuteri DSM 17938 with respect to their ability to influence the proliferation of antibiotic-resistant bacteria. Materials and methods. Cell-free extracts were obtained: 1) from L. reuteri cell...

One of the most promising methods for non-covalent inactivation of vaccine-producing microorganisms is the use of photoinactivation using riboflavin derivatives. The study used a dynamic combinatorial derivative of riboflavin - succinyl-maleinyl riboflavin. Corpuscular vaccines are divided into the following groups: from 2AB to 5AB - bacteria were...

Purpose: Wound-healing dipyridamole- and papaverine-based aerosols (D1/D2) as activators of the accumulation of cyclic adenosine monophosphate are promising drugs that can accelerate wound healing in wound processes of various origins. Methods: 128 rats were used in the study, including 38 in a pharmacological experiment on a model of stencil wound...

Medicni perspektivi. 2020;25(1):17-24 The purpose of this study was to evaluate the effect of supplementing the culture medium with ascorbic acid on the antimicrobial properties of Lactobacillus reuteri cell-free extracts (CFEs). CFEs were prepared using commercial strain L. reuteri DSM 17938 by culturing lactobacilli in its own disintegrated cell...

Introduction. In one patents demonstrated the riboflavin ability to inactivate vaccine strains of viruses and bacteria, as well as cancer cells during the production of vaccines . DNA and RNA within living organisms have specific aptamers or flavin places where Riboflavin and its derivatives are able to selectively join . Upon further blue light ir...

Introduction. The control over multi-resistant nosocomial strains of microorganisms has been becoming increasingly urgent in recent years. We suggest a new paradigm that is eliminating MDR bacteria death, but makes them sensitive to antibiotics. Based on our paradigm will be decreased and suppressed future selection of resistant bacterial strains....

Introduction: Wound-healing dipyridamole- and papaverine-based aerosols (D1/D2) as activators of the accumulation of cyclic adenosine monophosphate are promising drugs that can accelerate wound healing in wound processes of various origins. Materials and methods: 128 rats were used in the study, including 38 in a pharmacological experiment on a mod...

The article provides an overview of the current state of the use of TRIZ in the pharmaceutical industry and our R&D efforts in that area, based on TRIZ and computer mathematical modeling. Drug development is one of the most important research areas, which affects almost every family, and each one of us. However, nobody in the world has used TRIZ as...

Introduction. Mycobacterium tuberculosis urease (MTU), being an important factor of the bacterium viability and virulence, is an attractive target for anti-tuberculosis drugs acting by inhibition of urease activity. However, known urease inhibitors (phosphorodiamidates, hydroxamic acid derivatives and imidazoles) are toxic and/or unstable, that pre...

Objectives: Many adenocarcinomas have the ability to capture from an extracellular matrix the oligonucleotides and nanoparticles by pinocytosis, when the non-cancerous cells are not capable to capture the oligonucleotides and small liposomes. This provides selective accumulation of proposed protected oligonucleotides (fRNA) in cancer cells and als...

Introduction. M. tuberculosis urease (MTU) is an attractive target for chemotherapeutic intervention in tuberculosis by designing new safe and efficient enzyme inhibitors. A prerequisite for designing such inhibitors is an understanding of urease's three-dimensional (3D) structure organization. 3D structure of M. tuberculosis urease is unknown. Whe...

Introduction. Mycobacterium tuberculosis is the causative agent of tuberculosis that remains a serious medical and social health problem. Despite intensive efforts have been made in the past decade, there are no new efficient anti-tuberculosis drugs today, and that need is growing due to the spread of drug-resistant strains of M.tuberculosis. M. tu...

Introduction. Today, it is well established that almost 80% of all infectious diseases are caused by microorganisms that exist in the form of biofilms. Microorganisms in the biofilms acquire signs of increased resistance to antibiotics, disinfectants and other aggressive environmental factors, complicate the course of infectious diseases and play a...

Introduction Tuberculosis infection (TBI) is still one of the major health problems, despite of global intensive medical and pharmaceutical efforts as it is known, in the majority of immunocompetent individuals TBI is repressed by immune system, and as a result we can observe the latent TBI. The main danger hides in unpredictable activation process...

Background Many adenocarcinomas have the ability to capture from an extracellular matrix the oligonucleotides and nanoparticles by pinocytosis, when the non-cancerous cells are not capable to capture the oligonucleotides and small liposomes. This provides selective accumulation of proposed protected oligonucleotides (fRNA) in cancer cells and also...

Chlorophylls (a, b) are the porphyrin compounds and most common chemical in the plant’s world. In fact, these compounds are an obligatory intermediate product both in energy metabolism and in plant catabolism. At the same time, currently there are few pharmaceutical preparations on the pharmaceutical market based on chlorophylls. Dyes based on hydr...

Introduction The control over multi-resistant nosocomial strains of microorganisms has been becoming increasingly urgent in recent years. We suggest a new paradigm that eliminates killing or inhibiting the growth of bacteria. Excluding bacteria death supresses the selection of resistant strains of microorganisms. We have developed such non-metaboli...

Objectives: Currently, probiotics are increasingly used as the alternative to antibiotics as well as the preventive measures in humans. In particular, probiotics occupy a key position in the treatment of antibiotics-associated intestinal dysbiosis. A spore-forming microorganism lactobacillus Bacillus coagulans is one of the most promising probioti...

Introduction. In contemporary pharmaceutical practice among drugs used in dental gels are most effective, that are easily applied to the mucous and long held on the gums to form a protective film and prolonging therapeutic effect. Therefore it developed a new drug with a carbon dioxide Humulus Lupulus extract antimicrobial action in gel form for tr...

Introduction. The staye of intestinal microflora affects the work of the whole organism. When composition of normal ibtestine microflora changes, its restoration is required. In our days a wide variety of probiotic drugs are available on the market which can be used to solve this problem. Most bacteria having probiotic properties represent the fami...

IIntroduction. Diavenol - new extemporaneous gel for the treatment of venous ulcers. The gel contains a synergistic combination C. majus quinolizidine alkaloids and riboflavin. Activate tissue regeneration shown previously for riboflavin, and сheleretrin / sanguirythrine. The combination of the alkaloids and riboflavin pharmacologically fully justi...

Introduction. The widespread use of drugs with antimicrobial action has led to the formation of microorganism resistance against wide range of antibiotics. One of the approaches to dissolving this problem is the substances modification by inorganic bioactive ions in oder to initiate a controlled reaction in the bone tissues and provision of antimic...

Introduction. Quercetin is a flavone derivatives which known like a substances with vitamin activity, high antioxidant, antimutagenic and anticarcinogenic activity and many other types of biological activity. Wide usage of quercetin prevents their polyphenolic nature structure which does not allow a high bioavailability of pure quercetin when admin...

Immunotropic aspect of the Bacillus coagulans probiotic action Objective Currently, probiotics are increasingly used as the alternative to antibiotics as well as the preventive measures in humans. In particular, probiotics occupy a key position in the treatment of antibiotics-associated intestinal dysbiosis. A spore-forming microorganism lactobaci...

As a result of years’ research of the many research groups around the world able to understand the reason why it will be impossible to create really effective vaccine for the prevention of tuberculosis infection in the near future. The main reason for the impossibility creating such vaccine is an intracellular nature of tuberculosis. In fact, TB is...

This brief review concerns the basic principles of structural organization of multi-subunit bacterial ureases and formation of their quaternary structure. Urease is a nickel-containing enzyme (urea amidohydrolase, ЕС 3.5.1.5) that catalyses the hydrolysis of urea to get ammonia and carbamate which then decomposes with water to get ammonia and carbo...

Introduction. The key element in the formation of tuberculosis infection (TI) is the inability of alveolar macrophage to complete phagocytosis of mycobacteria absorbed by them, that caused by both features of the pathogens biology and the tissue macrophages. It is known, that M. tuberculosis is capable long-term persistence and proliferation in alv...

The purpose of this work is to study the influence of various degrees of protein’s acylation on lytic activity and other biological properties of Pseudomonas M6 bacteriophage. Materials and methods. The subject of the study were samples of the Pseudomonas O 12 bacteriophage, hereinafter named the M6 phage. Pure phage lines were obtained from indivi...

Introduction. One of the most promising probiotic including sporeforming microorganisms is a lactobacilli Bacillus coagulans (BC). In the spore form, it is resistant to technological processes and storage, it does not collapse under the influence of gastric juice and bile. After getting into the duodenum, the BC spores can germinate into vegetative...

Bacillus coagulans has an advantage over most other bacteria used as probiotics. It occupies an intermediate position between the genera Bacillusand Lactobacillus, is a spore-forming bacteria that produce lactic acid.This bacteria in the spores form can tolerate well technology processes, resistant to antibiotics and antiseptics, does not collapse...

Purpose: The biological activity (direct anticancer action in vitro) of combinatorial IL-2 succinylated derivatives was examined. Acylation of IL-2 was performed using succinic anhydride with various levels of acylation, with the formation of a complex assembly of many derivatives (self-assembled quasi-living structure).Methods: In the study, we us...

Целью исследований было получение новых, более растворимых и биологически активных производных эллаговой кислоты через ацилирование фенольных гидроксилов и получение супрамолекулярных комплексов с циклодекстринами, изучение способности синтезированных производных снимать резистентность микроорганизмов к аминогликозидным антибиотикам (на примере ген...

The results of the design and selection of compositions of antimicrobial ointments containing gen-tamicin as the main active substance and succinyl gallotannin as an antiresistant component. The concentration of the latter was 0.001%, had no direct antimicrobial effect, but blocked well the factors of microbial resistance and potentiated the effect...

A cosmetic and pharmaceutical formulation for rejuvenation and restoration of the skin, and methods of making this formulation. The cosmetic and pharmaceutical formulations comprise liposomes containing partially acylated proteins with a modification level of 0.1 to 10% of their mass, wherein the proteins are present in the form of a supramolecular...

Синтезовано ряд ацильованих похідних галотаніну промислового та елаготаніну з шишок вільхи сірої з різним ступенем заміщення фенольних гідроксилів. Синтез передбачав отримання суміші похідних з різними місцями заміщення фенольних гідроксилів та різною кількістю заміщень. Синтез ацильованих похідних проводили в умовах кип’ятіння, перекристалізацію о...

The goal was to establish a place of herpesviruses (HSV1, HSV2, HHV6, SMV, VZV, EBV) in the etiological structure of the eye’s diseases. The study involved 35 patients diagnosed with recurrent keratitis (15 patients) and viral uveitis (n = 20). Was carried out virological examination, including a study using fluorescent antibody scrapings from the...

The paper discusses the results of studies on physical and chemical properties of kvasi-living self-organizing insulin-based system, and on the effectiveness of its oral administration. The purpose of the studies was to modify positively charged amino acid residues into negatively charged residues of dicarboxylic acids. The process of bioorganic co...

Research has been conducted on the chemical and biological properties of combinatorial succinylated derivatives of interferon-γ with various levels of acylation, which create quasi-life, self-organizing ensembles. As a result of the research, it has been established that acylation by succinic anhydride of two lysines in the structure of interferon-...

Introduction: Since 1997, we have beenstudying the role of herpes viruses in thedevelopment of cancer and trying to deter-mine the reason tumors are invisible to theimmune system.Methods: In the work were used methodsof immunology and clinical study methods,clinical laboratory diagnostics.Results: From 1997 through 2010, 732people were studied. Amo...

Chemically modified antisense oligonucleotides with anti-cancer properties were synthesized, in which in the capacity of a main active ingredient, a formula of oligonucleotides from a mixture of the products of the hydrolysis of polynucleotides with molecular charges of the nucleotide bases changed to their opposite is used. This system is a self-o...

The crystal complex of β-cyclodextrin with succinic acid, intermediate product of hydrolysis reaction of succinic anhydride in the presence of β-cyclodextrin, was isolated and studied by X-ray analysis (monoclinic, space group P21, a = 15.1977(7) Å, b = 10.1763(5) Å, c = 20.6943(6) Å, β = 109.239(4)°, V = 3021.8(2) Å3, Z = 2, R 1 = 0.0359, wR 2 = 0...

An important place in the modern structure of the pharmaceutical market belongs to drugs – biotechnology products of various origins. These are such important drugs for patients' lives as recombinant insulins, interferons, interleukines, erythropoietins, etc. At the same time, statistic data of bringing such drugs, as well as a number of low-molecu...

N-arylimides of trans-aconitic acid were prepared by boiling the acid in a glacial acetic acid with N-arylamines. N-Arylamides of the trans-aconitic acid were obtained as intermediates in the course of the synthesis of the corresponding imides. The condensation of the trans-aconitic acid amides with hydrazine afforded arylamides of 1-amino-2,6-diox...

Effect of different levels of virions protein acylation on lytic activity, parameters of interaction with sensitive bacterial cells and other biological properties of phages has been studied in experiments on Klebsiella 45 bacteriophage. It was shown that at 5-, 10- and 20% levels of the phage sample acylation the specific lytic activity increased...

The antiproliferative activity of aconitylated (AIFN) and succinylated (SIFN) derivatives of recombinant interferon- alpha2b (IFN-alpha2b) was examined. Acylation of IFN-alpha2b was performed by succinic and cis-aconitic anhydrides. Antiproliferative properties of AIFN and SIFN were studied in vitro on the CaOv cell line, highly sensitive to IFN, a...

On an example of the rabbit antilegionella immunoglobulins (antLegIgRb) and monospecific human serum (antLegSerHum) the influence of a protein with different acylation level on specific and nonspecific activity of the above immunobiopreparations was investigated. It was determined, that 5 % level of chemical modification provides the highest level...

The data on the detection of the late antigen of herpes simplex virus (HSV) in patients with carcinoma of corpus uteri (CCU) are presented. It is shown that the HSV antigen was found in lymphocytes of CCU patients in 72% of cases, in erythrocytes – in 66% of cases, in tumor tissue – in 50% of cases and in the tissues adjacent to the tumor – in 17%...

The data on the detection of the late antigen of herpes simplex virus (HSV) in patients with carcinoma of corpus uteri (CCU) are presented. It is shown that the HSV antigen was found in lymphocytes of CCU patients in 72% of cases, in erythrocytes-in 66% of cases, in tumor tissue-in 50% of cases and in the tissues adjacent to the tumor-in 17% of cas...

The results of study of the antiviral activity and pharmacokinetics of phenylimide of cis-aconitic acid (PCAA) is presented. The 20% increase of the antiviral activity of PCAA incorporated into liposomes in comparison with the antiviral activity of the pure substance was shown. Liposomes with PCAA were tropic to lymphocytes and macrophages with max...

Condensation of arenesulfonamides with ethyl N-mesityloxaminate results in arylsulfonamides of N-mesitylhydoxamine acid. The efficiency of this reaction is compared with that of analogous reaction of diethyl oxalate with ethyl oxanilates. The dependences obtained is explained by the character of atomic charges on carbonyl carbon atoms of the oxamin...