Artem Guriev

• MD, PhD
• Head of Department at Siberian State Medical University

Introduction. 6,8-dimethyl-2-piperidinomethyl-2,3-dihydrothiazolo[2,3-F]xanthine is a promising substance-inducer of the monooxygenase system of hepatocytes, according to preclinical studies, increasing the detoxifying function of hepatocytes in models of acute hypobaric hypoxia, liver ischemia, unconjugated hyperbilirubinemia, toxic hepatitis. Pre...

Introduction. Biologically active substances of plant origin are the subject of study in the context of search and development of new pharmacologi-cal agents capable of influencing cholesterol metabolism in the body. The article presents the results of safety assessment, pharmacokinetics and mechanism of pharmacological activity of a new promising...

Introduction. α(1,2)-L-rhamno-α(1,4)-D-galactopyranosyluronane is a water-soluble heteropolysaccharide isolated from the rhizomes of calamus marsh ( Acorus calamus L.) and has antitumor, antimetastatic, immunomodulatory and hepatoprotective effects, protective effect on hematopoiesis suppressed during cytostatic treatment. Aim. Validation of the bi...

Introduction. The widespread use of heparin preparations for the treatment and prevention of venous thromboembolic complications is known. One of the low molecular weight heparin drugs successfully used in clinical practice is calcium nadroparin. The modern approach to assessing the quality of medicines involves the use of standards, in comparison...

Introduction. Cytochromes P450 depression is the reason for the low effectiveness of etiotropic and pathogenetic therapy of hepatitis. Recent experimental and clinical studies demonstrated the need for use of inducers of hepatocytes monooxygenase system to increase the effectiveness of treatment of chronic hepatitis and cirrhosis. 6,8-dimethyl-2-pi...

The presence of anti-polyethylene glycol (PEG) antibodies can limit the clinical efficacy of PEGylated drugs and cause anaphylactic reactions in patients. Monocytes/macrophages are effector cells involved in IgG-mediated passive systemic anaphylaxis. We studied the influence of human blood serum on the efficiency of uptake of PEGylated nanoparticle...

In male Syrian hamsters fed a synthetic high-fat diet enriched with cholesterol (0.3%), administration of a polysaccharide from birch leaves L-rhamnopyranosyl-6-O-methyl-D-galacturonan (3 g/100 g of diet) resulted in a decrease in total cholesterol levels, mainly due to the LDL fraction, triglycerides, and bile acids in blood serum; the content of...

Introduction. Dyslipidemia treatment in many cases requires carefully selected combination of lipid-lowering drugs including bile acid sequestrants. A promising compound is L-rhamnopyranosyl-6-O-methyl-galacturonan (L-RAG), a polysaccharide obtained from the birch leaves (Betula pendula L.).Aim. To evaluate the lipid-lowering activity of L-RAG admi...

Gramicidin S is a peptide antibiotic that has been widely used for more than 70 years. Gramicidin S is available in the form of tablets with a low dosage, which leads to possible deviations in the "Uniformity of dosage" parameter during manufacturing. Another limitation is the presence of lactose and sucrose in the formulation, which limits the d...

The in vitro addition of water-soluble polysaccharides isolated from the leaves of Crataegus sanguinea Pall. to culture of mouse peritoneal macrophages induced classical activation of antigen-presenting cells by increasing NO synthase activity and reducing arginase expression.

Introduction. Gramicidin S has been conventionally manufactured as buccal tablets. However, in the past decade, the interest in the development of spray formulations has been growing. Those formulations contain excipients that enhance the solubility of the antibiotic in water solutions. However, the real structure of gramicidin S containing sprays...

Addition of water-soluble polysaccharides isolated from Conium maculatum L. to the mouse peritoneal macrophage culture induces classical activation of antigen-presenting cells due to an increase in NO synthase activity and a decrease in arginase expression.

Myelotoxicity is a serious side effect of anticancer drugs. The search for drugs that can reduce the hematological complications of chemotherapy through modulation of hematopoietic stem cells is an urgent task of oncopharmacology. In the present study we showed that administration of Tussilago farfara L. polysaccharides to C57BL/6 mice treated with...

One of prospective methods for immunotherapy of tumors is modulation via immunological checkpoints, specifically, via the PD-1(CD279)/PD-L1(CD274) system. Interactions between tumor cell receptor (CD279) and the ligand on lymphocytes (CD274) leads to lymphocyte inactivation, which allows tumor escape from the immune control. Experiments on C57BL/6...

Hybrid microparticles based on an iron core and an amphiphilic polymeric shell have been prepared to respond simultaneously to magnetic and ultrasonic fields and variation in the surrounding pH to trigger and modulate the delivery of doxorubicin. The microparticles have been developed in four steps: (i) synthesis of the iron core; (ii) surface modi...

Hybrid microparticles based on an iron core and amphiphilic shell have been prepared to respond simultaneously to external (magnetic and ultrasounds field) and internal (pH) stimuli for delivery of the anticancer drug doxorubicin. The microparticles have been prepared in three main steps; including surface modification of the iron core followed by...

A possibility for correction of damaging effects of polychemotherapy on the intestinal epithelium with Tussilago farfara L. polysaccharides was studied on C57Bl/6 mice with Lewis lung carcinoma. The polysaccharides had protective and/or stimulating effects on the intestinal epithelium during polychemotherapy and promoted reparative regeneration in...

The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characte...

Currently, chemotherapy combined with surgery and radiation therapy is the most effective treatment for cancer. At the same time, the use of this method is accompanied by serious side effects caused by the lack of specificity of most chemotherapeutic agents. In this regard, the development of drug delivery systems (DDS) capable of addressing a chem...

4-oxo-4H-pyran-2.6-dicarboxylic acid (chelidonic acid, ChA) in the native state and in the complex with calcium [Ca(ChA)(H2O)3], named saucalchelin (CaChA), was isolated from the extract of Saussurea controversa leaves for the first time for the Asteraceae family. The structure of ChA was determined by NMR, MS and confirmed by X-ray analysis of its...

Целью данного исследования являлась разработка комплексной методики экстракции и очистки полисахаридов из листьев березы (Betula pendula Roth., Betula pubescens Ehrh. сем. березовых – Betulaceae) (ПСfB). В качестве критериев оценки влияния исследуемых параметров на получение целевых веществ изучали следующие характеристики: выход ПСfB (метод гравим...

Alfalfa is a very high-quality perennial leguminous grass with a long history of cultivation and application in traditional medicine in Russia. In addition, alfalfa is a valuable forage representative of the legume family, its acreage in Russia reaches 2.3–2.5 million ha. The polysaccharide complex (PSC) was isolated from the aerial part of alfalfa...

Using DNA comet assay we found that polysaccharides from Tussilago farfara L. reduced the intensity of polychemotherapy-induced apoptosis and DNA damage in bone marrow cells and small intestinal epithelium of C57Bl/6 mice, which attested to genoprotective properties of these polysaccharides.

The experiments on C57Bl/6 mice with Lewis lung carcinoma showed that addition of Tussilago farfara L. polysaccharides to conventional cisplatin/paclitaxel polychemotherapy moderated neutropenia caused by antitumor therapy and increased its efficiency. The stimulating effect of polysaccharides on the granulopoietic lineage cells is comparable with...

We characterized polysaccharide complexes from Centaurea scabiosa L. and Centaurea pseudomaculosа Dobrocz. We proposed the technique of sequential selection of water-soluble polysaccharides and pectin substances from the aerial parts of studied objects. We have discovered that the content of water-soluble polysaccharides in the aerial parts of C. s...

Tumor cells can maintain their growth via immunosuppression and escape from host antitumor immunity by controlling the PD-1/PD-L1 system. Expression of PD-L1 (CD274) is an inhibitory signal for T cells, while the increase in CD326 expression in the tumor tissue correlates with metastasis development. The experimental preparation on the basis of α(1...

The purpose of the study was to investigate the influence of polysaccharides Tussilagofarfara L. on erythroid sprout hematopoiesis, tumor growth and metastasis in mice with Lung cancer-67 during therapy with cisplatin and etoposide. It was found that the use of polysaccharides reduces myelosuppression induced by cytostatics of erythroidal sprout he...

Currently, much attention is paid to low-temperature atmospheric-pressure plasma as an agent for gentle antimicrobial decontamination. In this work, data on the efficiency of low-temperature plasma for antimicrobial decontamination of polyethylene terephthalate track membranes used as corneal implants in keratoplasty are presented. The obtained res...

Amethod for assaying sulfur hexafluoride in a new contrast agent by gas chromatography is described. Detection was with an electron capture detector (ECD). Sulfur hexafluoride was extracted from samples using heptane as the solvent. The method was validated in terms of the following parameters: specificity, linearity, correctness, stability, and in...

It was found that the addition of polysaccharides of Tussilago farfara L. to chemotherapy scheme "cisplatin + etoposide" lead to more pronounced inhibition of the Lewis lung carcinoma growth in mice due to the reduction of its mass in comparison with these parameters in animals treated according to the same scheme isolated. A use of polysaccharides...

A new GCMS-based method for quantitative determination of active substance of new contrast drug based on sulfur hexafluoride (SH) in the rat blood plasma is produced. A ratio plot of peak area of sulfur hexafluoride to peak area of internal standard toluene [y, S(SH)/S(toluene)] versus concentration of sulfur hexafluoride (x, µL/mL) was linear over...

Screening study of the effects of sweet flag (Acorus calamus L.) rhizome and clover (Trifolium pratense L.) aerial part on the production of NO by mouse macrophages was carried out. The polysaccharides were separated by ion exchange chromatography into fractions differing by monomeric composition and ramification type and were used in concentration...

The hepatoprotective properties of humic acids from lowland peat of Tomsk region were studied experimentally. It was established that native humic acids of peat (HAP) exhibited pronounced hepatoprotective activity against acute CCl4 hepatitis. The results showed that intragastric injection of HAP prevented damage from CCl4 to the metabolic and morp...

The antihypoxic activity of humic acids of Tomsk lowland peat was studied experimentally. It was established that native humic acids of peat (HAP) had pronounced antihypoxic activity after a preventive intragastric administration to mice, increasing the lifespan of animals and decreasing the lethality under hypobaric hypoxic and histotoxic hypoxia...

Diagnostic properties of humic acids of lowland wood-grass peat from Klyukvennoe deposit (Tomsk Region) were studied. Criteria for the identification and quality estimation of humic acids obtained from this source were established. The results showed the expediency of using these criteria as the standardization parameters.

We studied the effect of two pectic polysaccharides PS-B1-AG and PS-B2-RG that were contained in total polysaccharides extracted from Betula pendula leaves on NO production by mouse macrophages and the contribution of signaling molecules to macrophage activation by the test substances. Unlike the total sample, pectins produced a NO-stimulating effe...

The pharmacological properties of plant polysaccharides are reviewed and original experimental data on the properties of water-soluble polysaccharides isolated from Acorus calamus L. are presented. The possibility of using plant (in particular, Acorus calamus) polysaccharides to increase the effectiveness of anticancer treatment of transferred tumo...

Water-soluble polysaccharides (WSPS) were isolated from Calendula officinalis flowers using aqueous extraction followed by precipitation with EtOH and dialysis through a semi-permeable membrane. The WSPS were standardized with respect to uronic acid, protein, and nucleic acid content. Total WSPS were separated into five main components (PSC-1, PSC-...

The composition of water-soluble polysaccharides of the leaves Tussilago farfara L., possessing anti-allergic properties. It is shown that the polysaccharide complex consists of galacturonan I (33%) and neutral polysaccharides (67%).

Mutagenic properties of water soluble polysaccharides (WSPS) extracted from Acorus calamus L. have been studied. Neither a single intravenous injection nor a course intraperitoneal introduction of WSPS in a dose of 1/2 LD50 to mice of the CBA/CaLac line increases the level of cytogenetic disorders in the bone marrow cells. The investigation of WSPS...

A pectic polysaccharide was isolated from the rhizomes of Acorus calamus L. The main component of its carbohydrate chain was represented by residues of D-galacturonic acid (>85%). In addition, this polysaccharide contained residues of galactose, arabinose, xylose and rhamnose (<10%). Structural analysis of pectic polysaccharide from A.calamus L. wi...

We have studied the influence of water-soluble polysaccharides isolated from Tussilago farfara L. leaves, Betula verrucosa Ehrh. leaves, Calendula officinalis L. flowers, Acorus calamus rhizomes, Inula helenium L. rhizomes, overground part of Trifolium pretense L., and overground part of Artemisia absinthium L., on Th1 immune response induced by sh...

Were studied the effects of water-soluble plant polysaccharides isolated from pharmacopoeic raw material on anaphylactic shock and production of IgE and IgG1 by lymphocytes from mice immunized with ovalbumin. Course treatment with polysaccharides from coltsfoot, sweet flag, clover, Artemisia, marigold, and elecampane reduced animal mortality after...

An investigation of Kumazid medication influence on functional condition of experimental animal main organs and and systems, that were subjected to intragastric injection during three monthes, was made. It was determined that the Kumasid medication dosed 1—100 mkg/kg administrated to rats intragastically during three monthes doesn’t change internal...

Pathomorphological investigation of influence on rat’s internal conditions, that were subjected to injection of medication called kumasid for prophylactic immunization extracted from Cucumaria japonica was made. It was determined that the kumasid medication dosed 1—100 mkg/kg administrated to rats intragastically during three monthes doesn’t influe...

We studied the effects of polysaccharides from Siberian plants on the development of transplanted tumors under conditions of cytostatic treatment. Some mechanisms underlying the increase in the efficiency of antitumor therapy with flagroot (Acorus calamus) polysaccharides are shown.

Experiments on C57LB/6 mice with transplanted Luis lung carcinoma showed that the officinal Echinacea purpurea preparation did not influence the efficacy of cytostatic therapy. This echinacea preparation did not change the development of metastases and even stimulated the tumor growth. In contrast, a hydrophilic polysaccharide complex isolated from...

The structure of acorafuran (6-isopropyl-4-methyl-7,8-dihydro-6H-naphtho[1,8-bc]furan), a new cadalin-type sesquiterpenoid from Acorus calamus essential oil, was established using spectral data (NMR, mass spectrometry).