Artem Blagodatski

Artem Blagodatski
Moscow Institute of Physics and Technology | MIPT · Department of Molecular and Bio Physics

About

29
Publications
6,530
Reads
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639
Citations
Citations since 2016
15 Research Items
461 Citations
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100

Publications

Publications (29)
Article
WNT signaling plays paramount roles in organism development, physiology, and disease, representing a highly attractive target for drug development. However, no WNT-modulating drugs have been approved, with several candidates trudging through the early clinical trials. This delay instigates alternative approaches to discover WNT-modulating drugs. Na...
Article
Full-text available
Avian influenza is one of the largest known threats to domestic poultry. Influenza outbreaks on poultry farms typically lead to the complete slaughter of the entire domestic bird population, causing severe economic losses worldwide. Moreover, there are highly pathogenic avian influenza (HPAI) strains that are able to infect the swine or human popul...
Article
Full-text available
The Wnt signaling is one of the major pathways known to regulate embryonic development, tissue renewal and regeneration in multicellular organisms. Dysregulations of the pathway are a common cause of several types of cancer and other diseases, such as osteoporosis and rheumatoid arthritis. This makes Wnt signaling an important therapeutic target. S...
Article
Four new pterocarpans (6aR,11aR)-6a,11a-dihydrolespedezol A 2 (2), (6aR,11aR)-2-isoprenyl-6a,11a-dihydrolespedezol A 2 (3), (6aR,11aR,3′R)-6a,11a-dihydrolespedezol A 3 (4), (6aR,11aR,3′S)-6a,11a-dihydrolespedezol A 3 (5) and one new stilbenoid with 1,2-diketone fragment named bicoloketone (6) along with one previously known pterocarpen lespedezol A...
Article
Full-text available
Medicinal mushrooms have been used throughout the history of mankind for treatment of various diseases including cancer. Nowadays they have been intensively studied in order to reveal the chemical nature and mechanisms of action of their biomedical capacity. Targeted treatment of cancer, non-harmful for healthy tissues, has become a desired goal in...
Article
Full-text available
Moth-eye nanostructures are a well-known example of biological antireflective surfaces formed by pseudoregular arrays of nipples and are often used as a template for biomimetic materials. Here, we provide morphological characterization of corneal nanostructures of moths from the Bombycidae family, including strains of domesticated Bombyx mori silk-...
Article
Full-text available
Pro-proliferative oncogenic signaling is one of the hallmarks of cancer. Specific targeting of such signaling pathways is one of the main approaches to modern anti-cancer drug discovery, as opposed to more traditional search for general cytotoxic agents. Natural products, especially from marine sources, represent a largely untapped source of chemic...
Chapter
Corneal surfaces of terrestrial insects and other arthropods are covered with elaborate nanocoatings. Initially described as moth-eye nanostructures – paraboloid nipple-like evaginations regularly assembled on the lenses of some Lepidopterans – they were in recent years discovered to be omnipresent across insect lineages. In addition to the nipple-...
Article
Full-text available
Significance Corneal surfaces of some insects are coated with nipple-like nanostructures reducing the light reflection. Here we provide an extensive analysis of corneae across insect groups. Using atomic force microscopy, we discover a striking diversity of corneal nanocoatings, omnipresent in arthropods. These fascinating bionanostructures replica...
Article
Full-text available
Nanoscale nipple arrays covering the corneal surface of many insects provide antireflection properties and have inspired industrial applications. Based on visual inspection, the dense packing of these nanostructures was initially described to adopt a regular hexagonal order. However, Fourier analysis revealed lack of order over larger distances of...
Article
Full-text available
The Wnt/β-catenin signaling pathway is crucial in animal development from sponges to humans. Its activity in the adulthood is less general, with exceptions having huge medical importance. Namely, improper activation of this pathway is carcinogenic in many tissues, most notably in the colon, liver and the breast. On the other hand, the Wnt/β-catenin...
Article
Full-text available
Whirligig beetles (Gyrinidae) inhabit water surfaces and possess unique eyes which are split into the overwater and underwater parts. In this study we analyze the micro- and nanostructure of the split eyes of two Gyrinidae beetles genera, Gyrinus and Orectochilus. We find that corneae of the overwater ommatidia are covered with maze-like nanostruct...
Article
The immunoglobulin (Ig) genes of B cells are diversified at high rate by point mutations whereas the non-Ig genes of B cells accumulate no or significantly fewer mutations. Ig hypermutations are critical for the affinity maturation of antibodies for most of jawed vertebrates and also contribute to the primary Ig diversity repertoire formation in so...
Article
Full-text available
Directed evolution of proteins for improved or modified functionality is an important branch of modern biotechnology. It has traditionally been performed using various in vitro methods, but more recently, methods of in vivo artificial evolution come into play. In this review, we discuss and compare prokaryotic and eukaryotic-based systems of direct...
Article
Full-text available
Fourier spectra of nucleotide sequences from chicken (Gallus gallus) chromosome 15 were obtained. Periodical structures of several fragments of the Ig light chain locus were characterized. The Fourier spectra of the locus and its parts (variable and constant segments, enhancer, and sequences supposedly associated with somatic hypermutation) were sh...
Article
Full-text available
Author Summary It remains an open question how AID-mediated gene diversification is targeted to the immunoglobulin loci. Here we define a cis-acting sequence, named DIVAC for diversification activator, which is required for hypermutation of the Ig light chain gene and sufficient to activate hypermutation at various non-Ig loci in the DT40 B cell li...
Data
(A) Targeting strategy of the GFP2 reporter into four different loci on chromosome 15. (B) Targeting strategy of the GFP2 reporter into the A-MYB, RAD52, BACH2, and BCL6 loci. The targeting strategies used for the insertions into the AID and the RMD1 loci were described previously [2],[29]. (1.87 MB EPS)
Data
Comparison of GFP gene expression levels analyzed by semi-quantitative RT-PCR of primary transfectants belonging to the stepwise deletions series of the ‘W’ fragment. (2.27 MB EPS)
Data
Green fluorescence decrease in individual primary transfectants. (A) GFP2 reporter in the vicinity of the IgL locus. (B) GFP2 reporter in non-Ig loci. (0.11 MB XLS)
Article
Full-text available
A large number of cDNA inserts were sequenced from a high-quality library of chicken bursal lymphocyte cDNAs. Comparisons to public gene databases indicate that the cDNA collection represents more than 2,000 new, full-length transcripts. This resource defines the structure and the coding potential of a large fraction of B-cell specific and housekee...

Questions

Question (1)
Question
I have tried Trizol and Quiagen RNEasy kits to isolate RNA from waterfowl feces (I added influenza virus in serial dilutions to some feces samples as a positive control). However none of the samples was positive on RT-PCR (controls with RNA isolation from same amount of pure virus culture without feces were clearly positive). Does someone have an experience of detection and isolation of influenza virus from bird feces?

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Projects

Projects (2)
Project
Screen of the small molecules from abyssal hydrobionts, plants and microalgae.