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Introduction
Post Transcriptional and Post translational modifications regulating plant immunity
Additional affiliations
July 2009 - October 2014
Publications
Publications (38)
N6-methyladenosine (m6A) is a common and dynamic epitranscriptomic modification in eukaryotic RNAs, affecting stability, splicing, translation, and degradation. Recent technological advancements have revealed the complex nature of m6A modifications, highlighting their importance in plant and animal species. The m6A modification is a reversible proc...
The INDETERMINATE DOMAIN (IDD) family belongs to a group of plant-specific transcription factors that coordinates plant growth/development and immunity. However, the function and mode of action of IDDs during abiotic stress, such as salt, are poorly understood. We used idd4 transgenic lines and screened them under salt stress to find the involvemen...
Linker H1 histones play an important role in animal and human pathogenesis, but their function in plant immunity is poorly understood. Here, we analyzed mutants of the three canonical variants of Arabidopsis H1 histones, namely H1.1, H1.2 and H1.3. We observed that double h1.1h1.2 and triple h1.1h1.2h1.3 (3h1) mutants were resistant to Pseudomonas...
The use of beneficial microbes to mitigate drought stress tolerance of plants is of great potential albeit little understood. We show here that a root endophytic desert bacterium, Pseudomonas argentinensis sp. SA190, enhances drought stress tolerance in Arabidopsis. Transcriptome and genetic analysis demonstrate that SA190-induced root morphogenesi...
Linker H1 histones play an important role in animal and human pathogenesis, but their function in plant immunity is poorly understood. Here, we analyzed mutants of the three canonical variants of Arabidopsis H1 histones, namely H1.1, H1.2 and H1.3. We observed that double h1.1h1.2 and triple h1.1h1.2h1.3 (3h1) mutants were resistant to Pseudomonas...
Oxylipins constitute a huge class of compounds produced by oxidation of long-chain unsaturated fatty acids either chemically (by radicals such as reactive oxygen species, ROS) or enzymatically (by lipoxygenases, LOX; cyclooxygenases, COX; or cytochrome P450 pathways). This process generates fatty acids peroxides, which can then be further modified...
Plants have developed a robust transcription machinery to combat potential pathogenic organisms. One of the hallmarks of early immune responses is the activation of the WRKY transcription factors post infection. Specific WRKYs proteins from Arabidopsis are known substrates of MAPK pathway to mediate the flg22 elicited early immunity.
In the current...
Global warming has become a critical challenge to food security, causing severe yield losses of major crops worldwide. Conventional and transgenic breeding strategies to enhance plant thermotoler-ance are laborious and expensive. Therefore, the use of beneficial microbes could be an alternative approach. Here, we report that the root endophyte Ente...
Strawberry ( Fragaria x ananassa ) is an economically important high-value crop that is susceptible to three most devastating pathogens with different lifestyles — a necrotrophic fungus Botrytis cinerea causing grey mold, a hemibiotrophic oomycete Phytophthora cactorum causing crown/root rot, and a biotrophic fungus Podosphaera aphanis causing powd...
Global warming has become a critical challenge to food safety, causing severe yield losses of major crops worldwide. Heat acclimation empowers plants to survive under extreme temperature conditions but the potential of beneficial microbes to make plants thermotolerant has not been considered so far. Here, we report that the endophytic bacterium Ent...
Arabidopsis PR1 is a salicylic acid (SA) inducible marker gene for systemic acquired resistance (SAR). However, the regulation of PR1 in plants is poorly understood. In this study, we showed that AtWRKY50 transcription factor binds to two promoter elements of PR1 via its DNA binding domain. Interestingly, the DNA-binding sites for AtWRKY50 deviate...
Signalling pathways play vital roles as determinants of almost all the molecular processes inside a eukaryotic cell. They are more often considered to be the link between extracellular and intracellular environmental cues. Gene silencing pathways have emerged to be involved in regulation of stress responses and developmental processes. However, ver...
Recent evidence suggests that the ubiquitin-proteasome system (UPS) is involved in several aspects of plant immunity and a range of plant pathogens subvert the UPS to enhance their virulence. Here we show that proteasome activity is strongly induced during basal defense in Arabidopsis. Mutant lines of the proteasome subunits RPT2a and RPN12a suppor...
Recent evidence suggests that the ubiquitin-proteasome system (UPS) is involved in several aspects of plant immunity and a range of plant pathogens subvert the UPS to enhance their virulence. Here, we show that proteasome activity is strongly induced during basal defense in Arabidopsis and mutant lines defective in proteasome subunits RPT2a and RPN...
Mitogen-activated protein kinase (MAPK) cascades are central signaling pathways activated in plants after sensing internal developmental and external stress cues. Knowledge about the downstream substrate proteins of MAPKs is still limited in plants. We screened Arabidopsis WRKY transcription factors as potential targets downstream of MAPKs, and con...
Nuclear localization of WRKY46: (A) Confocal laser scanning micrographs of Arabidopsis Col-0 mesophyll protoplasts transfected with the indicated WRKY46-YFP constructs. To visualize the nucleus, nuclear DNA was stained with 1 μg mL-1 of 4′,6-Diamidino-2-Phenylindole (DAPI) in the presence of 0.05 % Triton-X100 (to permeabilize the membranes). Scale...
MicroRNA biogenesis requires AtDRB1/HYL1 protein for processing and maturation of miRNA precursors. The AtDRB1/HYL1 protein associates with AtDCL1 and accurately processes pri-miRNAs first to pre-miRNAs and finally to mature miRNAs. The dephosphorylation of AtDRB1/HYL1 protein is very important for the precise processing of miRNA precursors. The mo...
Mitogen activated protein kinase (MAPK) pathway is one of the most conserved signaling cascade in plants regulating a plethora of cellular processes including normal growth and development, abiotic and biotic stress responses. The perception of external cues triggers the phosphorylation of three tier MAPKKK-MAPKK-MAPK cascade which finally modifies...
Transient infiltrations in tobacco are commonly used in plant studies but the host response to different disarmed Agrobacterium strains is not fully understood. The present study shows that the pre-treatment with disarmed Agrobacterium tumefaciens strain GV3101 primes the defense response to subsequent infection by Pseudomonas syringae in Nicotiana...
The canonical mitogen activated protein kinase (MAPK) signaling pathway plays a vital role in carrying out the normal growth and development of the plant. The pathway, connecting the upstreams signal with the downstream target is considered to be linear, mostly starting with a MAPKKK and ending in a MAPK.
Here we report a novel interaction between...
Background
Mitogen activated protein kinase (MAPK) cascade is an important signaling cascade that operates in stress signal transduction in plants. The biologically active monoterpenoid indole alkaloids (MIA) produced in Catharanthus roseus are known to be induced under several abiotic stress conditions such as wounding, UV-B etc. However involveme...
Activation of MAP Kinases by MeJA treatment in C. roseus. 6–8 weeks old C. roseus plants were subjected to MeJA treatment at the mentioned concentrations. Control plant was given the mock treatment (Ethanol) in the same fashion as that of MeJA treatment. Activity of MAP kinase was assessed by in-gel kinase assay using MBP as a substrate polymerized...
Phylogenetic relationship of CrMPK3 to other plant MAPKs. The amino acid sequence of the proteins used for generating phylogenetic tree is listed in GenBank database under the following accession numbers: OsMPK3 (A2XFC8), OsMPK6 (ABO69383), NtF4 (X83880), SIMK (X66469), PsD5 (X70703), AtMPK6 (D21842), ZmMPK5 (AB016802), WIPK (D61377), AtMPK3 (D2183...
Nuclear localization of CrMPK3-GFP upon wounding. C. roseus leaf discs transiently transformed with CrMPK3-GFP cloned in pCAMBIA1303 vector and observed under confocal microscope 10 min after wounding. A-B: CrMPK3-GFP fluorescence and bright field respectively. C: DAPI staining shows position of nucleus.
Deduced amino acid sequence of CrMPK3. Amino acid sequence of CrMPK3 highlighting different subdomains of MAP kinase. Conserved TEY motif present between subdomain VII and VIII is underlined.
Plasmolysis of C. roseusleaf cells to confirm the subcellular localization of CrMPK3-GFP fusion protein. Plasmolysis was achieved by incubating the cells in 1 M mannitol solution for 2 hours.
CrMPK3 activation in response to wounding, UV, MeJA treatments. Total protein extracts (200 μg) was subjected to immunoprecipitation with the pTEpY antibody. The immunoprecipitated complex was electrophoresed on SDS 10%(w/v) polyacrylamide gel and immunoblot was performed using anti-AtMPK3. MeJA mock treatment was performed by applying solvent only...
Specificity of CrMPK3 antibody. Immunoblot was performed using CrMPK3 antibody against total protein extract of Arabidopsis Atmpk3 mutant line and wild type plants (Col 0).
List of genes and primer pairs for Q RT-PCR.
We here report in Pisum sativum system a novel protein-protein interaction of β-subunit of heterotrimeric G-proteins (PsGβ) with a Mitogen activated protein kinase (PsMPK3) during cDNA library screening by yeast-two-hybrid assay. The transcript of these two genes also showed co-regulation under abscisic acid (ABA) and methyl jasmonate (MeJA) treatm...
Questions
Questions (3)
I am trying to do a LR clonase rxn between pDONR-zoe harbouring my gene and pDEST-N110 (His-10 bacterial expression system, Dyson et al). Even though I get some colonies on LB+amp plates. But then they are not induced by IPTG in BL21 (DE3) pLysS cells. I am doubting whether the very first step of cloning is compatible and whether the cloning is in frame??
I dried a SDS-PAGE gel without staining it before. Can I stain the gel now? If yes, how?? It is totally dried on a thin Whatman paper.
Protein gets bound to beads but does not come out....:(
