Arnaud Dubois

• PhD
• Professor (Full) at Institut d'Optique Graduate School

A time-domain optical coherence tomography technique is introduced for high-resolution B-scan imaging in real-time. The technique is based on a two-beam interference microscope with line illumination and line detection using a broadband spatially coherent light source and a line-scan camera. Multiple (2048) A-scans are acquired in parallel by scann...

An optical technique called line-field confocal optical coherence tomography (LC-OCT) is introduced for high-resolution, noninvasive imaging of human skin in vivo. LC-OCT combines the principles of time-domain optical coherence tomography and confocal microscopy with line illumination and detection using a broadband laser and a line-scan camera. LC...

Line-field confocal optical coherence tomography (LC-OCT) is a recently introduced technique for ultrahigh-resolution vertical section (B-scan) imaging of human skin in vivo. This work presents a new implementation of the LC-OCT technique to obtain horizontal section images (C-scans) in addition to B-scans. C-scan imaging is achieved with this dual...

We describe an original microscope for high-resolution optical coherence tomography applications. Our system is based on a Linnik interference microscope with high-numerical-aperture objectives. Lock-in detection of the interference signal is achieved in parallel on a CCD by use of a photoelastic birefringence modulator and full-field stroboscopic...

We have developed a white-light interference microscope for ultrahigh-resolution full-field optical coherence tomography of biological media. The experimental setup is based on a Linnik-type interferometer illuminated by a tungsten halogen lamp. En face tomographic images are calculated by a combination of interferometric images recorded by a high-...

The image contrast and probing depth of optical methods applied to in vivo skin could be improved by reducing skin scattering using the optical clearing method. The aim of this study was to quantify, from line‐field confocal optical coherence tomography (LC‐OCT) 3D images, the modifications of skin scattering properties in vivo during optical clear...

This article introduces an innovative line-field confocal optical coherence tomography (LC-OCT) system based on tandem interferometry, featuring a focus-tunable lens for dynamic focusing. The principle of tandem interferometry is first recalled, and an analytical expression of the interferometric signal detected is established in order to identify...

La LC-OCT est une récente technique d’imagerie fournissant des vues en 2 ou 3 dimensions de l’intérieur de la peau avec une résolution micrométrique. La LC-OCT est basée sur un microscope optique interférométrique en éclairant la peau avec une ligne de lumière à large spectre. Cette technique d’imagerie non invasive est utilisée en dermatologie pou...

LC-OCT enabled manual tracking of individual cell motion in the upper dermal microvessels. Blood flow velocity was on average 2.5 times higher (173 vs 70 µm/s) when measured at 40 vs 8 frames per second.

LC-OCT enables 3D in vivo skin imaging at the cellular level. It can be used for the characterization of various skin pathologies. New technical developments promise to further augment the diagnostic capabilities of LC-OCT. Full-text article not available; see video presentation

Line-field confocal optical coherence tomography (LC-OCT) is a non-invasive optical imaging technique based on a combination of the principles of optical coherence tomography and reflectance confocal microscopy with line-field illumination, which can generate cell-resolved images of the skin in vivo. This article reports on the LC-OCT technique and...

Line-field confocal optical coherence tomography (LC-OCT) is an imaging modality based on a combination of time-domain optical coherence tomography and reflectance confocal microscopy. LC-OCT provides three-dimensional images of semi-transparent samples with a spatial resolution of ∼ 1 μm. The technique is primarily applied to in vivo skin imaging....

Line-field confocal optical coherence tomography (LC-OCT) is a non-invasive optical imaging technique based on a combination of the optical principles of optical coherence tomography and reflectance confocal microscopy with line-field illumination, which can generate cell-resolved images of the skin, in vivo, in vertical section, horizontal section...

LC-OCT is an optical technique that provides three-dimensional images of the skin, in vivo , with cellular resolution (1 μm). LC-OCT can be used in dermatology for non-invasive diagnosis and study of various lesions including cancers.

Significance: Line-field confocal optical coherence tomography (LC-OCT) is a recently introduced high-resolution imaging modality based on a combination of low-coherence optical interferometry and reflectance confocal optical microscopy with line illumination and line detection. Capable of producing three-dimensional (3D) images of the skin with c...

Line-field confocal optical coherence tomography (LC-OCT) is an optical modality that provides three-dimensional (3D) images of the skin at cellular resolution. Confocal Raman microspectroscopy (CRM) is a label-free optical technique that can provide point measurement of the molecular content of the skin. This work presents a method to co-localize...

Although numerous pathologies are associated with abnormal skin deposits, these remain poorly described, as accurate characterization continues to present a challenge for dermatologists. Their submicrometer size as well as their diverse chemistry require various characterization tools.We aim to exemplify characterization of endogenous and exogenous...

Epidermal three-dimensional (3D) topography/quantification has not been completely characterized yet. The recently developed line-field confocal optical coherence tomography (LC-OCT) provides real-time, high-resolution, in-vivo 3D imaging of the skin. This pilot study aimed at quantifying epidermal metrics (epidermal thicknesses, dermal-epidermal j...

Diagnosis based on histopathology for skin cancer detection is today’s gold standard and relies on the presence or absence of biomarkers and cellular atypia. However it suffers drawbacks: it requires a strong expertise and is time-consuming. Moreover the notion of atypia or dysplasia of the visible cells used for diagnosis is very subjective, with...

This paper reports on the latest advances in line-field confocal optical coherence tomography (LC-OCT), a recently invented imaging technology that now allows the generation of either horizontal (x × y) section images at an adjustable depth or vertical (x × z) section images at an adjustable lateral position, as well as three-dimensional images. Fo...

Background Line‐field confocal optical coherence tomography (LC‐OCT) is a non‐invasive optical technique recently developed for skin examination in vivo . It provides real‐time, high‐resolution vertical images with an isotropic resolution of ~1 µm and a penetration depth of ~500 µm. Objectives Study goals were to qualitatively/quantitatively chara...

Line-field confocal optical coherence tomography (LC-OCT) is an imaging technique in which A-scans are acquired in parallel through line illumination with a broadband laser and line detection with a line-scan camera. B-scan imaging at high spatial resolution is achieved by dynamic focusing in a Linnik interferometer. This paper presents an LC-OCT d...

The objective was to compare LC-OCT images with histological sections of the same area, in order to validate a new method for in vivo and non-invasive quantification of superficial dermis thickness.

Background: Line-field confocal optical coherence tomography (LC-OCT) is an imaging technique providing "optical biopsies" of the skin in real time and non-invasively. At a center optical wavelength of 1.3 µm, this innovative technology can be applied to dermo-cosmetic product development due to both high image resolution (~2 µm) and sufficient pe...

Line-field Confocal Optical Coherence Tomography (LC-OCT) is an innovative imaging technique that combines the advantages of both Reflectance Confocal Microscopy (RCM) and OCT, thus providing optical biopsies of the skin down to the reticular dermis in real-time and non-invasively. With its high axial and lateral resolutions, LC-OCT can be applied...

La tomographie par cohérence optique, plus communément appelée par son acronyme anglais OCT ( optical coherence tomography ), est une technique d’imagerie non invasive des milieux biologiques, à résolution micrométrique, dont l’impact le plus remarquable concerne l’ophtalmologie. L’OCT « plein champ » est une approche originale de l’OCT, inventée p...

Line-field confocal optical coherence tomography (LC-OCT) operating in two distinct spectral bands centered at 770 nm and 1250 nm is reported, using a single supercontinuum light source and two different line-scan cameras. B-scans are acquired simultaneously in the two bands at 4 frames per second. Greyscale representation and color fusion of the i...

Advances in optical coherence tomography (OCT) for application in dermatology are reported. Full-field OCT and line-field OCT are compared in terms of spatial resolution, penetration and acquisition rate. In vivo imaging of skin lesions is demonstrated with good similarity to histology.

A compact high‐speed full‐field optical coherence microscope has been developed for high‐resolution in vivo imaging of biological tissues. The interferometer, in the Linnik configuration, has a size of 11×11×5 cm³ and a weight of 210 g. Full‐field illumination with low‐coherence light is achieved with a high‐brightness broadband light‐emitting diod...

This paper reports on advances in optical coherence tomography (OCT) for application in dermatology. Full-field OCT is a particular approach of OCT based on white-light interference microscopy. FF-OCT produces en face tomographic images by arithmetic combination of interferometric images acquired with an area camera and by illuminating the whole fi...

Dual-band line-field confocal optical coherence tomography is reported for cross-sectional imaging in real-time at 760 nm and 1270 nm center wavelengths simultaneously. Differences in absorption and scattering in skin tissues, in vivo, is revealed.

A compact simplified algorithm for digital detection of the amplitude and phase of the interferometric signal delivered by a two-wave interferometer with sinusoidal phase modulation is presented. The algorithm consists of simple mathematical combinations of four frames obtained by integration by a camera of the time-varying intensity in an interfer...

Full-field optical coherence microscopy (FFOCM) is an optical technique, based on low-coherence interference microscopy, for tomographic imaging of semi-transparent samples with micrometer-scale spatial resolution. The differences in refractive index between the sample and the immersion medium of the microscope objectives may degrade the FFOCM imag...

Full-field optical coherence microscopy (FF-OCM) is an imaging technique that provides cross-sectional views of the subsurface microstructure of semitransparent objects. The technology is based on low-coherence interference microscopy, which uses an area camera for en face imaging of the full-field illuminated object. FF-OCM benefits from the later...

High-resolution full-field optical coherence microscopy (FF-OCM) is demonstrated using a single broadband light-emitting diode (LED). The characteristics of the LED-illumination FF-OCM system are measured and compared to those obtained using a halogen lamp, the light source of reference in FF-OCM. Both light sources yield identical performance in t...

Full-field optical coherence microscopy (FF-OCM) with isotropic spatial resolution of 0.5 μm (in water), at 700 nm center wavelength, is reported. A theoretical study of the FF-OCM axial response is carried out for maximizing the axial resolution of the system, considering the effect of optical dispersion. The lateral resolution is optimized by usi...

An original single-objective, full-field optical coherence microscopy system is reported that is capable of imaging both the phase and the amplitude of semi-transparent samples over a field of view of with an axial sectioning resolution of 1.5 μm. A special stack acquisition arrangement ensures optimal reachable imaging depth. Several phase-shiftin...

We present an ultrahigh resolution spectral-domain optical coherence tomography imaging system using a broadband superluminescent diode light source emitting at a center wavelength of 1.3µm. The light source consists of two spectrally shifted superluminescent diodes that are coupled together into a single mode fiber. The effective emission power sp...

Full-field optical coherence tomography (FF-OCT) is a biomedical imaging technique based on white-light interference microscopy. FF-OCT produces tomographic images by arithmetic combination of interferometric images acquired with an area camera and by illuminating the whole field to be imaged with low-coherence light. The major interest for FFOCT l...

Full-field optical coherence microscopy (FF-OCM) is an established optical technology based on low-coherence interference microscopy for high-resolution non-invasive three-dimensional imaging of semi-transparent samples. We present an extension of the technique setting up an achromatic imaging system over a spectral range extending from 530 nm to 1...

We propose a 3D imaging technique based on the combination of full-field swept-source optical coherence microscopy (FF-SSOCM) with low spatial coherence illumination and a special numerical processing that allows for numerically focused coherent-noise-free imaging without mechanical scanning in longitudinal or transversal directions. We show, both...

Full-field optical coherence microscopy is an established optical technology based on low-coherence interference microscopy for high-resolution imaging of semitransparent samples. In this Letter, we demonstrate an extension of the technique using a visible to short-wavelength infrared camera and a halogen lamp to image in three distinct bands cente...

poster présenté par Flavie Gillant

Full-field optical coherence microscopy (FF-OCM) is a recent optical technology based on low-coherence interference microscopy for semi-transparent sample imaging with ∼ 1 μm spatial resolution. FF-OCM has been successfully applied to three-dimensional imaging of various biological tissues at cellular-level resolution. The contrast of FF-OCM image...

Optical coherence tomography (OCT) is an imaging technique used to visualize in real time the internal structure of biological tissues in a non-invasive manner, without preliminary preparation, with a spatial resolution close to that of optical microscopy. The technique can also be applied to the imagery of semi-transparent materials. In the medica...

Circumventing the limit imposed by diffraction is a major issue in the instrumental development to realize finer resolutions in biological samples. With STED microscopy, we exploit the molecular transitions of the fluorescent marker to image well below the Rayleigh criterion. Also in combination with STED, we propose to use an alternative technique...

Full-field optical coherence microscopy (FF-OCM) is a biomedical imaging technique based on white-light interference microscopy. FF-OCM produces tomographic images in the en face orientation by arithmetic combination of interferometric images acquired with an area camera and by illuminating the whole field to be imaged with low-coherence light. The...

We present an ultrahigh resolution spectral-domain optical coherence tomography imaging system using a broadband superluminescent diode light source emitting at a center wavelength of 1.3 μm. The light source consists of two spectrally shifted superluminescent diodes that are coupled together into a single mode fiber. The effective emission power s...

Séminaire à l'Université de Tunis El Manar, Tunisie. Invitation par le Pr. Ezeddine Sediki

poster présenté par Karen Perronet

Full-field optical coherence microscopy (FF-OCM) and optically sectioned fluorescence microscopy are two imaging techniques that are implemented here in a novel dual modality instrument. The two imaging modalities use a broad field illumination to acquire the entire field of view without raster scanning. Optical sectioning is achieved in both imagi...

Glaucoma is a disease of the optic nerve that is usually associated with an increased internal pressure of the eye and can lead to a decreased vision and eventually blindness. It is the second leading cause of blindness worldwide with more than 80 million people affected and approximately 6 million blind. The standard clinical treatment for glaucom...

Full-field optical coherence tomography (FF-OCT) is a recent optical imaging technology based on low-coherence interference microscopy for imaging of semi-transparent samples with ~1 µm spatial resolution. FF-OCT produces en-face tomographic images obtained by arithmetic combination of interferometric images acquired by an array camera. In this pap...

We present a first evaluation of the results of the NOUGAT project which has been funded by the French National Research Agency through the 2008 edition of its TecSan program. The objective of the project is to conceive and realise an innovative device for ocular surgery by ultrafast pulse laser for glaucoma surgery assisted by an in situ imaging s...

poster présenté par Karen Perronet