Arnaud Buhot

Arnaud Buhot
Atomic Energy and Alternative Energies Commission | CEA · INAC Institute for Nanoscience and Cryogenics

Ph. D. in Theoretical Physics

About

115
Publications
10,378
Reads
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2,041
Citations
Introduction
Arnaud Buhot is researcher from SyMMES (UMR 5819 CEA, CNRS, Univ. Grenoble Alpes, France) since 2002. He is head of the CREAB team (circ. 30 pers) since 2014. With an initial background in soft matter, statistical physics and biophysics, he has also a strong experience in biomolecular interactions, Surface Plasmon Resonance imaging detection and the development of biosensors, aptasensors and opto-electronic noses/tongues (> 60 publications and 13 PhD students supervised).
Additional affiliations
March 2002 - present
CEA Grenoble
Position
  • Researcher
October 1999 - January 2002
University of Oxford
Position
  • PostDoc Position
Education
September 1995 - May 1999
September 1992 - June 1995
Ecole normale supérieure de Lyon
Field of study
  • Theoretical Physics

Publications

Publications (115)
Article
Full-text available
Loop-mediated isothermal amplification (LAMP) is an exponential amplification method of DNA strands that is more and more used for its high performances. Thanks to its high sensitivity and selectivity, LAMP found numerous applications from the detection of pathogens or viruses through their genome amplification to its incorporation as an amplificat...
Article
Photobiological effects are known to greatly depend on the wavelength of the incident photons that define the nature of the activated chromophores. A growing number of experimental data show that considering the effect of complex light sources as a sum of the effects of monochromatic exposures can be misleading. Indeed, the combined exposure to sev...
Article
Full-text available
The last three decades have witnessed an increasing demand for novel analytical tools for the analysis of gases including odorants and volatile organic compounds (VOCs) in various domains. Traditional techniques such as gas chromatography coupled with mass spectrometry, although very efficient, present several drawbacks. Such a context has incited...
Article
Differential sensing of proteins based on cross-reactive arrays and pattern recognition is a promising technique for the detection and identification of proteins. In this study, a rational biomimetic strategy has been used to prepare sensing materials capable of discriminating structurally similar proteins, such as deletion and point mutants of a c...
Article
Full-text available
Small molecules are ubiquitous in nature and their detection is relevant in various domains. However, due to their size, sensitive and selective probes are difficult to select and the detection methods are generally indirect. In this study, we introduced the use of melting curve analysis of aptachains based on split-aptamers for the detection of ad...
Article
This work depicts the original combination of electrochemiluminescence (ECL) and bipolar electrochemistry (BPE) to map in real‐time the oxidation of silicon in microchannels. We fabricated model silicon‐PDMS microfluidic chips, optionally containing a restriction, and monitored the evolution of the surface reactivity using ECL. BPE was used to remo...
Article
The localized functionalization of pores and channels of micrometric and sub‐micrometric sizes is a bottleneck in surface chemistry. A method for the regioselective chemical functionalization of planar pores is presented, that are, restrictions in microfluidic channels, here made of SiO2‐coated silicon. This strategy, based on bipolar electrochemis...
Book
Full-text available
Chapter 1 devoted to the development of biomimetic olfactory biosensors and electronic noses. Authors introduce the mechanism of smell and odor discrimination principles and describe the main components of the olfactory system that are currently used as raw sensing materials for the design of biosensors namely the olfactory receptors and some other...
Article
Although cardiac pathologies are the major cause of death in the world, it remains difficult to provide a reliable diagnosis to prevent heart attacks. Rapid patient care and management in emergencies are critical to prevent dramatic consequences. Thus, relevant biomarkers such as cardiac troponin and natriuretic peptides are currently targeted by c...
Article
Full-text available
The combination of bipolar electrochemistry (BE), as a wireless electrochemical approach, and of electrochemiluminescence (ECL) as an imaging readout is a successful strategy with a wide range of analytical applications. However, small conductive entities such as micrometric and nanometric objects are particularly difficult to polarize by BE since...
Article
Full-text available
In the 90s, the development of a novel single molecule technique based on nanopore sensing emerged. Preliminary improvements were based on the molecular or biological engineering of protein nanopores along with the use of nanotechnologies developed in the context of microelectronics. Since the last decade, the convergence between those two worlds h...
Article
Improving the sensitivity of plasmonic optical fiber sensors constitutes a major challenge as it could significantly enhance their sensing capabilities for the label-free detection of biomolecular interactions or chemical compounds. While many efforts focus on developing more sensitive structures, we present here how the sensitivity of a sensor can...
Article
The analysis of volatile organic compounds (VOCs) is an important issue in various domains. For this, electronic noses (eN) are very promising as novel analytical tools that are portable, inexpensive, and efficient for reliable and rapid analyses. Recently, we have demonstrated that surface plasmon resonance imaging (SPRI) is especially interesting...
Article
Full-text available
Artificial noses are broad-spectrum multisensors dedicated to the detection of volatile organic compounds (VOCs). Despite great recent progress, they still suffer from a lack of sensitivity and selectivity. We will review, in a systemic way, the biomimetic strategies for improving these performance criteria, including the design of sensing material...
Article
Recent advances in SPR-imaging detection in gas phase have led to the development of opto-electronic nose (opto-eN) requiring the need for optical sensitivity characterization of such devices. Understanding of the optical contributions will have an implicit relevance on sensitivity enhancement of SPR-imaging in gas phase valuable to improve the per...
Article
Full-text available
The development of sensitive methods for in situ detection of biomarkers is a real challenge to bring medical diagnosis a step forward. The proof-of-concept of a remote multiplexed biomolecular interaction detection through a plasmonic optical fiber bundle is demonstrated here. The strategy relies on a fiber optic biosensor designed from a 300 µm d...
Article
Full-text available
This review summarizes recent advances in micro- and nanopore technologies with a focus on the functionalization of pores using a promising method named contactless electro-functionalization (CLEF). CLEF enables the localized grafting of electroactive entities onto the inner wall of a micro- or nano-sized pore in a solid-state silicon/silicon oxide...
Book
This review summarizes recent advances in micro- and nanopore technologies with a focus on thefunctionalization of pores using a promising method named contactless electro-functionalization (CLEF). CLEF enables the localized grafting of electroactive entities onto the inner wall of a microor nano-sized pore in a solid-state silicon/silicon oxide me...
Article
Nowadays, the analysis of volatile organic compounds (VOCs) is very important in various domains. In the last decades, electronic noses have emerged as promising alternatives to traditional analytical methods. Nevertheless, their wide use is still limited by their performances such as low selectivity. Herein, we developed an optoelectronic nose usi...
Article
Bipolar electrochemistry (BPE) is a powerful method based on the wireless polarization of a conductive object that induces the asymmetric electroactivity at its two extremities. A key physical limitation of BPE is the size of the conductive object because the shorter the object, the larger is the potential necessary for sufficient polarization. Mic...
Conference Paper
Electronic Nose (eN) systems use sensor arrays containing multiple probes to generate fingerprints for the sensitive and selective detection of Volatile Organic Compounds (VOCs). In this paper, we analyzed the effect of VOC concentrations and of temperatures from 5°C to 35°C on the equilibrium response profiles. Fingerprint patterns for 1-butanol a...
Article
Remote detection by surface plasmon resonance (SPR) is demonstrated through microstructured optical arrays of conical nanotips or micropillars. Both geometries were fabricated by controlled wet chemical etching of bundles comprising several thousands of individual optical fibers. Their surface was coated by a thin gold layer in order to confer SPR...
Article
Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of...
Article
Monitoring volatile organic compounds (VOCs) is an important issue, but difficult to achieve on a large scale and on the field using conventional analytical methods. Electronic noses (eNs), as promising alternatives, are still compromised by their per-formances due to the fact that most of them rely on a very limited number of sensors and use datab...
Article
Full-text available
Pre-transfusion bedside compatibility test is mandatory to check that the donor and the recipient present compatible groups before any transfusion is performed. Although blood typing devices are present on the market, they still suffer from various drawbacks, like results that are based on naked-eye observation or difficulties in blood handling and...
Article
Full-text available
The small molecules detection remains a challenge for diagnostics. Due to their size, difficulties arise in finding selective and sensitive probes as well as developing appropriate detection format based on sandwich and/or signal amplification assays. In this study, we combine multiple strategies: a) the use of aptamer probes, short oligonucleotide...
Article
Full-text available
Quantification of biomarkers closest to the patient remains an impressive challenge for the development of personalized medicine. The detection of D-dimer, a fibrin degradation product, indicative of deep vein thrombosis, pulmonary embolism or stroke is a particular biomarker of interest. In this study, we developed a quantitative assay based on th...
Article
Full-text available
The detection of small molecules impacts various fields however their small size and low concentration are usually the cause of limitations in their detection. Thus, the need for biosensors with appropriate probes and signal amplification strategies is required. Aptamers are appropriate probes selected specifically against small targets like adenos...
Article
Full-text available
Nowadays, there is a strong demand for the development of new analytical devices with novel performances to improve the quality of our daily lives. In this context, multisensor systems such as electronic tongues (eTs) have emerged as promising alternatives. Recently, we have developed a new versatile eT system by coupling surface plasmon resonance...
Article
Full-text available
Small molecules (MW < 1000 Da) represent a large class of biomarkers of interest. Recently, a new class of biosensors has been emerging thanks to the recognition properties of aptamers, short DNA or RNA single strands, selected against such small molecular targets. Among them, an adenosine-specific aptamer has been largely described and used due to...
Article
The process of agglutination is commonly used for the detection of biomarkers like proteins or viruses. The multiple bindings between micrometer sized particles, either latex beads or red blood cells (RBCs), create aggregates that are easily detectable and give qualitative information about the presence of the biomarkers. In most cases, the detecti...
Article
Full-text available
Polymer translocation is a promising strategy for the next-generation DNA sequencing technologies. The use of biological and synthetic nano-pores, however, still suffers from serious drawbacks. In particular, the width of the membrane layer can accommodate several bases at the same time making difficult accurate sequencing applications. More recent...
Article
Ionizing radiations induce clustered damage sites known to be severely challenging for the cell's repair machinery. In the present study, we have grafted on a biochip four synthetic oligonucleotide duplexes containing either a single 8-oxoguanine, a single 2'-deoxyribose abasic site or two different clustered damages containing these two lesions on...
Article
Full-text available
The detection of small molecules by biosensors remains a challenge for diagnostics in many areas like pharmacology, environment or homeland security. The main difficulty comes from both the low molecular weight and low concentrations of most targets, which generally requires an indirect detection with an amplification or a sandwich procedure. In th...
Article
Electronic noses/tongues (eN/eT) have emerged as promising alternatives for analysis of complex mixtures in the domain of food and beverage quality control. We have recently developed an electronic tongue by combining surface plasmon resonance imaging (SPRi) with an array of non-specific and cross-reactive receptors prepared by simply mixing two sm...
Article
Full-text available
In current protocol, a combinatorial approach has been developed to simplify the design and production of sensing materials for the construction of electronic tongues (eT) for protein analysis. By mixing a small number of simple and easily accessible molecules with different physicochemical properties, used as building blocks (BBs), in varying and...
Article
Full-text available
We have recently developed a novel approach which greatly simplified the design and production of sensing materials for the construction of electronic tongues (eT) by mixing a handful of building blocks (BBs) and allowing the mixtures to self-assemble. This approach has two major advantages: it allows a rapid growth in diversity of the sensing rece...
Article
Full-text available
Hepatitis C virus infection leads to liver disease whose severity can range from mild to serious lifelong illness. However the parameters involved in the evolution of the disease are still unknown. Among other factors, the virus-elicited antibody profile is suspected to play a role in the outcome of the disease. Analysis of the relationship between...
Article
Full-text available
Aptamers are selected DNA ligands that target biomolecules such as proteins. In recent years, they are showing an increasing interest as potential therapeutic agents or recognition elements in biosensor applications. In both cases, the need for characterizing the mating between the target and the aptamer either in solution or immobilized on a surfa...
Article
Full-text available
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS). A physical understanding of the hybridization process helps to determine the accuracy of these technologies. The goal of a widespread research program is to develop reli...
Article
Licking the problem: A new type of electronic tongue was constructed by self-assembly on the surface of a surface plasmon resonance (SPR) imaging prism with combinations of simple building blocks. The resulting combinatorial array was used to detect proteins in solution by using SPR imaging to monitor the interactions. The signal from each spot was...
Article
The use of biological-probe-modified solid-state pores in biosensing is currently hindered by difficulties in pore-wall functionalization. The surface to be functionalized is small and difficult to target and is usually chemically similar to the bulk membrane. Herein, we demonstrate the contactless electrofunctionalization (CLEF) approach and its m...
Article
The possibility to detect low abundant DNA point mutations is essential for early cancer diagnosis and/or prognosis. Furthermore, in order to be less invasive, the somatic mutations are not only sought in tumor extract samples but also from body fluids or stools rendering their content even more diluted compared to the wild type sequences. In this...
Article
Platinating agents are commonly prescribed anticancer drugs damaging DNA. Induced lesions are recognized by a wide range of proteins. These are involved in cellular mechanisms such as DNA repair, mediation of cytotoxicity or chromatin remodeling. They therefore constitute crucial actors to understand pharmacology of these drugs. To expand our knowl...
Article
The viscosity η of polymer melts experimentally scales with the length L of the chains as a power law with an exponent b ≈ 3.4 larger than the prediction b = 3 from de Gennes' theory of reptation. This long-standing controversy is revisited within the repton and necklace models. Exact results from the rigid chain dynamics allow us to predict the le...
Article
DNA microarrays find applications in an increasing number of domains where more quantitative results are required. DNA being a charged polymer, the repulsive interactions between the surface of the microarray and the targets in solution are increasing upon hybridization. Such electrostatic penalty is generally reduced by increasing the salt concent...
Article
The secretions of molecules by cells are of tremendous interest for both fundamental insights studies and medical purposes. In this study, we propose a new biochip-based approach for the instantaneous monitoring of protein secretions, using antibody production by B lymphocytes cultured in vitro. This was possible thanks to the Surface Plasmon Reson...
Article
In molecular biology, formamide (FA) is a commonly used denaturing agent for DNA. Although its influence on DNA duplex stability in solution is well established, little is known about immobilized DNA on microarrays. We measured thermal denaturation curves for oligonucleotides immobilized by two standard protocols: thiol self-assembling and pyrrole...
Article
Full-text available
Base excision repair (BER) is the major mechanism for the correction of damaged nucleobases resulting from the alkylation and oxidation of DNA. The first step in the BER pathway consists of excision of the abnormal base by several