Arif Istiaq

Arif Istiaq
Kyushu University | Kyudai · Department of Stem Cell Biology

PhD in Developmental Neurobiology (Candidate)

About

34
Publications
14,663
Reads
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250
Citations
Citations since 2017
32 Research Items
241 Citations
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2017201820192020202120222023020406080
2017201820192020202120222023020406080
Introduction
Investigating the mechanisms that induce stemness in terminally differentiated somatic cells. Currently working on ribosome-mediated cellular reprogramming of somatic cells.
Additional affiliations
April 2020 - September 2022
Kyushu University
Position
  • Special Research Student PhD
October 2018 - March 2022
Kumamoto University
Position
  • PhD Student
April 2018 - September 2018
Microbial Genetics and Bioinformatics Laboratory, University of Dhaka
Position
  • Research Assistant
Description
  • Bovine Mastitis Meta-genomics Project
Education
October 2018 - October 2022
Kumamoto University
Field of study
  • Developmental Neurobiology
January 2014 - February 2018
March 2011 - October 2016

Publications

Publications (34)
Article
Full-text available
The ribosome, which is present in all three domains of life, plays a well-established, critical role in the translation process by decoding messenger RNA into protein. Ribosomal proteins, in contrast, appear to play non-translational roles in growth, differentiation, and disease. We recently discovered that ribosomes are involved in reverting cellu...
Article
Tsukushi (TSK), a leucine-rich peptidoglycan in the extracellular compartment, mediates multiple signaling pathways that are critical for development and metabolism. TSK regulates signaling pathways that eventually control cellular communication, proliferation, and cell fate determination. Research on TSK has become more sophisticated in recent yea...
Poster
bacterial ribosome stimuli promotes stem-cell like characteristics in mouse primary fibroblast
Article
Full-text available
Tsukushi (TSK) proteoglycan dysfunction leads to hydrocephalus, a condition defined by excessive fluid collection in the ventricles and lateral ventricular enlargement. TSK injections into the LV at birth are effective at rescuing the lateral ventricle (LV). TSK regulates the activation of the Wnt signaling to facilitate the proper expansion of the...
Article
Full-text available
Neurogenesis persists in selected regions of the adult mouse brain; among them, the ventricular-subventricular zone (V-SVZ) of the lateral ventricles represents a major experimental paradigm due to its conspicuous neurogenic output. Postnatal V-SVZ neurogenesis is maintained by a resident population of neural stem cells (NSCs). Although V-SVZ NSCs...
Article
Full-text available
Arsenotrophic bacteria play an essential role in lowering arsenic contamination by converting toxic arsenite [As (III)] to less toxic and less bio-accumulative arsenate [As (V)]. The current study focused on the qualitative and electrocatalytic detection of the arsenite oxidation potential of an arsenite-oxidizing bacteria A. xylosoxidans BHW-15 (r...
Article
Full-text available
Previously we reported that, lactic acid bacteria (LAB) can induce human dermal fibroblast (HDF) cells to form multipotent cell clusters which are able to transdifferentiate into three germ layer derived cell lineages. Later on, we confirmed that ribosome is responsible for the LAB-induced transdifferentiation and ribosomes from diverse organisms c...
Chapter
In 2006, Takahashi and Yamanaka reported the generation of mouse and human induced pluripotent stem cells (iPS cells) by retroviral gene transduction of four transcription factors, famously known as Yamanaka factors (OCT4, SOX2, Klf4, and c-Myc) (Takahashi et al., 2007; Takahashi and Yamanaka, 2006). iPS cells show stemness properties (self-renewal...
Article
Although ribosomes are generally known to be a translational machinery, some ribosomal proteins also have accessory functions involving early development and differentiation. Previously, we reported that ribosome incorporation into human dermal fibroblasts generated embryoid body-like cell clusters, altered cellular fate, and differentiated into ce...
Chapter
In 2006, Takahashi and Yamanaka reported the generation of mouse and human induced pluripotent stem cells (iPS cells) by retroviral gene transduction of four transcription factors, famously known as Yamanaka factors (OCT4, SOX2, Klf4, and c-Myc) (Takahashi et al., 2007; Takahashi and Yamanaka, 2006). iPS cells show stemness properties (self-renewal...
Article
Full-text available
Introduction. Effective sanitation strategies for poultry farms require an appropriate selection of the disinfectant based on the contaminants present and their sensitivity to the disinfectants. Aim. The current study investigated the prevalence of streptococci/micrococci in poultry farms of Bangladesh and the efficacy of commercial disinfectants (...
Preprint
Full-text available
The milk of lactating cows presents a complex ecosystem of interconnected microbial communities which can influence the pathophysiology of mastitis. We hypothesized possible dynamic shifts of microbiome compositions with the progress of different pathological states of mastitis (CM, Recurrent CM; RCM, Subclinical Mastitis; SCM) determined by its fa...
Article
Full-text available
Bovine clinical mastitis (CM) is one of the most prevalent diseases caused by a wide range of resident microbes. The emergence of antimicrobial resistance in CM bacteria is well-known, however, the genomic resistance composition (the resistome) at the microbiome-level is not well characterized. In this study, we applied whole metagenome sequencing...
Article
Secreted proteoglycan molecule Tsukushi (TSK) regulates various developmental processes, such as early body patterning and neural plate formation by interacting with major signaling pathways like Wnt, BMP, Notch etc. In central nervous system, TSK inhibits Wnt signaling to control chick retinal development. It also plays important roles for axon gu...
Article
Specialized microenvironment, or neurogenic niche, in embryonic and postnatal mouse brain plays critical roles during neurogenesis throughout adulthood. The subventricular zone (SVZ) and the dentate gyrus (DG) of hippocampus in the mouse brain are two major neurogenic niches where neurogenesis is directed by numerous regulatory factors. Now, we rep...
Preprint
Full-text available
The bovine clinical mastitis (CM) milk is a large reservoir for diverse groups of resistomes, which play important roles in the pathogenesis of mastitis, but little is known about the concurrence of CM microbiome signature and its associated resistomes. Here we deciphered the total resistance (antibiotics and metals resistance, biofilm formation, q...
Preprint
Full-text available
The bovine clinical mastitis (CM) milk is a large reservoir for diverse groups of resistomes, which play important roles in the pathogenesis of mastitis, but little is known about the concurrence of CM microbiome signature and its associated resistomes. Here we deciphered the total resistance (antibiotics and metals resistance, biofilm formation, q...
Conference Paper
Full-text available
Background: Bovine mastitis is considered as one of the most economically important diseases of livestock, and diverse groups of microbiotas are associated with the disease. Understanding the microbial colonization and interaction during mastitis in cattle is necessary to elucidate the progression of pathogenesis and development of sustainable prev...
Article
Full-text available
Milk microbiomes significantly influence the pathophysiology of bovine mastitis. To assess the association between microbiome diversity and bovine mastitis, we compared the microbiome of clinical mastitis (CM, n = 14) and healthy (H, n = 7) milk samples through deep whole metagenome sequencing (WMS). A total of 483.38 million reads generated from b...
Preprint
Full-text available
Milk microbiomes impose a significant influence on the pathophysiology of bovine mastitis. To assess the association, we compared the microbiome of clinical mastitis (CM) and healthy (H) milk samples through whole metagenomic deep sequencing. A total of 483.38 million reads from both metagenomes were analyzed by PathoScope and MG-RAST, and mapped t...
Preprint
Full-text available
Background and Objectives: Due to huge demand and availability of Banana, innovative cost effective method is necessary to promote and smoothen the banana production among farmers commercially mitigating the demand. Method and Materials: In this study, we feed cow dung mixture along with Urea, TSP, MoP, water to the distal part of rachis after cutt...
Article
Full-text available
Chromosomal co-existence of metal and antibiotic resistance genes in bacteria offers a new perspective to the bacterial resistance proliferation in contaminated environment. In this study, an arsenotrophic bacterium Achromobacter xylosoxidans BHW-15, isolated from Arsenic (As) contaminated tubewell water in the Bogra district of Bangladesh, was ana...
Article
Due to huge demand and availability of Banana, innovative cost effective method is necessary to promote and smoothen the banana production among farmers commercially mitigating the demand. Method and Materials in this study, we feed cow dung mixture along with Urea, Triple Super Phosphate (TSP), Murate of Potash (MoP), water to the distal part of r...
Article
Full-text available
Hevea brasiliensis, a plant belonging family Euphorbiaceae. In Brazil this plant is not only use for medicinal purpose but also for cosmetics purpose. The present study was aimed to study analgesic and antidiarrheal activity of methanol extract of Hevea brasiliensis. Analgesic activity was evaluated by acetic acid- induced writhing method and antid...
Poster
Full-text available
Bovine mastitis micrbiome characterization using different bioinformatics tools
Data
Whole genome sequence of novel arsenite oxidizing Achromobacter xylosoxidans BHW-15 NCBI ACCESSION PZMK00000000
Presentation
Full-text available
Abstract ID- 139 Using several pertinent bioinformatic tools, we analyzed 102 mitochondrial cytochrome-b gene sequences of four identified species of Tenualosa genus along with geographical references for phylogeny establishment and further explored a complete mitochondrial DNA sequence analysis for genetic composition, intergenic spacer, genetic...
Poster
Full-text available
Typhoid is a very harmful disease caused by Salmonella typhi which is a Gram-negative bacterium. It was found that Salmonella typhi can overcome the defense of the immune system with the help of Vi polysaccharide antigen. Vi polysaccharide is a cell surface antigen. It is anti-phagocytic and antiopsonic. It reduces the secretion of S. typhi-induced...
Article
Full-text available
Background: The study was conducted to evaluate the in vitro antimicrobial activity, cytotoxic, and membrane stabilization activities, and in vivo antiemetic and antipyretic potentials of ethanolic extract, n-hexane and ethylacetate soluble fractions of Spilanthes paniculata leaves for the first time widely used in the traditional treatments in Ban...
Article
Full-text available
The purpose of the research is to find out the comparative cytotoxic potentials of Ipomoea Pes-Caprae and Murraya koenigii fractions (crude methanol, Pet ether, Chloroform) by using brine shrimp mortality assay. These two plant extracts were tested at concentration 400, 200, 100, 50, 25, 12.5, 7.25, 3.125 µg/ml. The extracts of both plants showed r...

Questions

Questions (2)
Question
Sphere formation is known among normal or cancer stem cells. But whats the underlying mechanism of cells to come together and make sphere?
Question
The desired gene to be expressed is aioBA of ~3.1kb . I have already tried pET100/D Topo vector. The insert was PCR amplified (forward primer with CACC overhang at 5’-end) with Pfu Polymerase (to get blunt end product) , gel purified to get a single, discrete band of desired size. After cloning (at recommended molar ratio) and transformation few colonies(5-10) were on LB/Amp plate. Colonies were analyzed by Colony PCR with orientation specific primers (T7 reverse-binds to vector and our designed primer aioBA_exp_F-binds to insert). Unfortunately, no band of desired size amplicon were there. I also miniprepped some transformant colonies and restriction analysis was done using PstI (cut once in vector and once in insert) but digestion pattern suggesting an empty vector. So far, I am assuming that the ligation of vector and insert is not happening. I have done cloning for several times, but was never successful.
So before I switch to another vector, I want to get some suggestions.
Thank you in advance.

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Projects

Projects (8)
Project
Mechanism of Cellular reprogramming by ribosome