Apostolos Kalivas

Apostolos Kalivas
Hellenic Agricultural Organization - Demeter | ELGO · Insitute of Plant Breeding and Phytogenetic Resourses

Researcher, Ph.D.

About

85
Publications
23,523
Reads
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1,185
Citations
Additional affiliations
January 2007 - December 2010
Education
October 2003 - November 2009
Aristotle University of Thessaloniki
Field of study
  • Plant Breeding
September 2000 - September 2003
Aristotle University of Thessaloniki
Field of study
  • Agriculture
September 1994 - November 1999
Aristotle University of Thessaloniki
Field of study
  • Agriculture

Publications

Publications (85)
Article
Full-text available
Hemp (Cannabis sativa L.) is a rapidly growing plant with multipurpose uses, and the optimal combination of yield and quality of hemp products (fibers, inflorescences, or seeds) may provide economic opportunities to uncover the full spectrum of its capabilities. The presented experimentation took place over seven years (2017-2023) in Greek climatic...
Article
Full-text available
Citation: Tsaliki, E.; Moysiadis, T.; Toumpas, E.; Kalivas, A.; Panoras, I.; Grigoriadis, I. Evaluation of Greek Tobacco Varieties (Nicotiana tabacum L.) Grown in Different Regions of Greece. Agriculture 2023, 13, 1394. Abstract: Tobacco (Nicotiana tabacum L.) is an important industrial crop grown in more than one hundred countries worldwide, with...
Article
Full-text available
Dill (Anethum graveolens L.) is an aromatic herb widely used in the food industry, with several commercial cultivars available with different qualitative characteristics. Commercial cultivars are usually preferred over landraces due to their higher yield and also the lack of improved landraces than can be commercialized. In Greece, however, traditi...
Article
Full-text available
Eggplant is an economically important vegetable with a potential for functional food production, mainly due to its high fruit antioxidant capacity. The goal of the present study was to investigate the main physicochemical and antioxidant parameters, and assess the bioactive profiles, of 19 eggplant genotypes of diverse origin, including Greek comme...
Article
On a P-poor, calcareous soil, three upland cotton (Gossypium hirsutum L.) cultivars (ST 402, ST 405, Zeta 2) were tested for 2 years under three P rates (0, 13.1, 26.2 kg P ha −1). Leaf traits (SPAD values; specific leaf area, SLA; carbon isotope discrimination, Δ; 15 N natural abundance, δ 15 N) and elements (N, P, K, C, Na, Zn) along with arbuscu...
Article
Full-text available
Salt stress is considered as one of the most frequent factors limiting plant growth and productivity of crops worldwide. The aim of the study was to evaluate physiological and biochemical responses of nine diverse tomato genotypes exposed to salt stress. In this regard, four-week-old seedlings of one modern variety, five landraces, the salt-sensiti...
Article
Parsley ( Petroselinum crispum ) aromatic, edible leaves are used fresh or dried as a herb, but also for their pharmaceutical properties. Given the profound concern to high nutritious and pharmaceutical value of superior plant’s germplasm, a survey is presented in this study on the phenotypic, genetic and phytochemical characterization of fifteen G...
Article
Full-text available
Background: Summer squash (Cucurbita pepo: Cucurbitaceae) are a popular horticultural crop for which there is insufficient genomic and transcriptomic information. Gene expression atlases are crucial for the identification of genes expressed in different tissues at various plant developmental stages. Here, we present the first comprehensive gene exp...
Article
Full-text available
Abstract: A selection of sesame (Sesamum indicum L.) landraces of different eco-geographical origin and breeding history have been characterized using 28 qualitative morpho-physiological descriptors and 7 EST-SSR markers coupled with HRM analysis. The most variable qualitative traits that could efficiently discriminate landraces, as revealed by the...
Article
Full-text available
Farmers’ interest in renewable raw materials such as hemp (Cannabis sativa L.) fibres has recently increased, but hemp productivity is strongly affected by genotype and environment conditions. A 3-year field experiment was conducted under Mediterranean environment in northern Greece to evaluate the productivity (regarding fibres and seeds) of six m...
Article
Full-text available
Greece is a country that primarily cultivates tobacco. Over the years and the increase of inputs to conventional tobacco cultivation and the groundwater nitrate pollution, producers and industry began to look at organic farming. However, for the field crop of tobacco knowledge is limited to inorganic fertilization only and it is necessary now to fi...
Article
Full-text available
As organic tobacco is a consumer choice, it is necessary to explore the proper fertilization program with environmentally friendly practices. Α field experiment lasting 3 growing seasons established at the Agricultural University of Athens in order to examined the effect of comparison between organic and inorganic fertilization on (flue-cured) Vi...
Article
Full-text available
Chloride is a micronutrient that has a major influence on the development of tobacco, more specifically its quality, that is on its chemical properties and on the overall structure of the leaves. Α greenhouse pot experiment was carried out at the Agricultural University of Athens in 2018, in order to investigate changes in agronomic and quality pro...
Article
Full-text available
A selection of tomato ( Solanum lycopersicum L.) genotypes with diverse origin and breeding history including 33 landraces, eight modern varieties and two commercial hybrids has been characterized using a set of 25 qualitative descriptors and six quantitative traits. A wide range of variation was evident for the majority of traits, highlighting the...
Article
Tobacco is an economically important crop with leaves that are a significant source of aromatic, bioactive compounds such as phenolics and alkaloids. In the present study un- biased transcriptomics and metabolomics approaches were employed to identify and quantify individual changes in transcript and metabolite profiles in leaves of three oriental...
Article
Full-text available
Beans are one of the most important staple crops in the world. Runner bean (Phaseolus coccineus L.) is a small-scale agriculture crop compared to common bean (Phaseolous vulgaris). Beans have been introduced to Europe from the Central America to Europe and since then they have been scattered to different geographical regions. This has resulted in t...
Article
Full-text available
Cannabis sativa L. seeds have been an important source of protein, oil, and dietary fiber for human and animals. Currently, there is a growing interest in the commercial products of these seeds, which are recognized as a legitimate source of medicaments, cosmeceuticals, and nutraceuticals. The objective of this study was to investigate the nutritio...
Article
Full-text available
Α wide selection of tomato genotypes with diverse origin and breeding history (14 modern varieties, 71 landraces and 22 commercial hybrids) has been initially genotyped with a selection of highly informative simple sequence repeat (SSR) markers and two SCAR markers originally developed for resistance against two main fungal tomato diseases. Our dat...
Article
Full-text available
Cucurbita pepo contains two cultivated subspecies each of which encompasses four fruit-shape morphotypes (cultivar-groups). The Pumpkin, Vegetable Marrow, Cocozelle, and Zucchini Groups are of subsp. pepo and the Acorn, Crookneck, Scallop, and Straightneck Groups are of subsp. ovifera. Recently, a de novo assembly of the C. pepo subsp. pepo Zucchin...
Article
Full-text available
In this study, we evaluated crop productivity and physiology during the hydroponic cultivation of medium-sized and cherry tomato crops, using two experimental greenhouses. Of the greenhouses, one used dye-sensitized solar cell (DSSC) technology for covering material, whilst the other, a conventional one (CONV), was covered using diffusion glass as...
Article
Full-text available
To further understand the impact of grafting on fruit characteristics and to comprehend the mechanisms involved in graft-induced changes we studied homo- and hetero- grafted Cucurbita pepo cultivars (cv.) that vary in fruit size and shape. C. pepo cv. ‘Munchkin’ and cv. ‘Big Moose’ as well as cv. ‘Round green’ and cv. ‘Princess’ were homo-grafted a...
Article
Pea (Pisum sativum, L.) is an important legume crops in the world especially for the cool-season, used both for human food and animal feed having high protein and vitamin content. As with many crops Gene Banks hold significant collections which in many cases have not been assessed through molecular genotyping. Yet, this pre-breeding step is highly...
Preprint
Full-text available
To further understand the impact of grafting on fruit characteristics and to comprehend the mechanisms involved in graft-induced changes we studied homo- and hetero- grafted Cucurbita pepo cultivars (cv.) that vary in fruit size and shape. C. pepo cv. ‘Munchkin’ (small fruit) and cv. ‘Big Moose’ (large fruit) as well as cv. ‘Round green’ (round fru...
Preprint
Full-text available
To further understand the impact of grafting on fruit characteristics and to comprehend the mechanisms involved in graft-induced changes we studied homo- and hetero- grafted Cucurbita pepo cultivars (cv.) that vary in fruit size and shape. C. pepo cv. ‘Munchkin’ (small fruit) and cv. ‘Big Moose’ (large fruit) as well as cv. ‘Round green’ (round fru...
Article
Full-text available
Nutrients in the form of fertilizers and/or other additives such as amino acids, dramatically influence plant development and growth, plant nutrient composition and the level of soil pollution. Moreover, the treatment of soil microbiota is emerging as a new strategy in plant breeding to achieve desirable traits. Thus, integrated study of fertilizer...
Article
The genetic basis of fruit size and shape was investigated for the first time in Cucurbita species and genetic loci associated with fruit morphology have been identified. Although extensive genomic resources are available at present for tomato (Solanum lycopersicum), cucumber (Cucumis sativum), melon (Cucumis melo) and watermelon (Citrullus lanatus...
Article
Farmer field schools (FFS) represent an alternative lifelong learning approach aimed at enhancing farmers' competences and skills through nonformal education. In this study we examined if farmers' participation in FFS is motivated by their social-psychological needs and whether these needs can be fulfilled through participation in FFS. Findings rev...
Conference Paper
More than 25 years after the first implementation of Farmer Field Schools (FFS), there is a rich corpus of evidence that participation in FFS improves farmers’ knowledge, skills, and competencies. On the other hand, several studies converge to show that FFS, by strengthening group action, have the potential to build-up social capital among particip...
Article
Upland cotton (Gossypium hirsutum L.) is an important fiber crop species, which is intensively plagued by a plethora of phytopathogenic fungi such as Fusarium oxysporum f. sp. vasinfectum (Fov) causing severe wilt disease. Resistance gene analogs (RGAs) are the largest class of potential resistance (R) genes depicting highly conserved domains and s...
Article
Oriental tobacco varieties have greater aroma value and consumer demand for these varieties is now greater than ever. Yet, our knowledge on morphological and genetic diversity within diverse gene pools of cultivated oriental tobacco (Nicotiana tabacum L.) varieties is limited. Phenotypic and genetic analysis of these genotypes is important for the...
Article
Full-text available
Key message The selected germplasm of Ficus carica established in a gene bank collection will be useful for conservation and management and important for fig tree breeding programs. Abstract Advancement in plant breeding is assisted by accurate information on genetic diversity and structure. A collection of ninety fig tree (Ficus carica L.) cultiva...
Conference Paper
Full-text available
Τα υποτροπικά φυτά παρουσιάζουν πολύ καλή ανάπτυξη και καρποφορία σε κάποιες θερμές περιοχές της Ελλάδας και ιδιαίτερα στην Κρήτη αρκετά από αυτά έχουν εγκατασταθεί σε εμπορικές καλλιέργειες με πολύ καλά οικονομικά αποτελέσματα. Η φραγκοσυκιά (Opuntia ficus indica) μπορεί να αποτελέσει ιδιαίτερα ενδιαφέρουσα εναλλακτική καλλιέργεια και να συμβάλει...
Presentation
Full-text available
Η πρόοδος στη βελτίωση των φυτών επικουρείται από ακριβείς πληροφορίες για τη γενετική ποικιλότητα. Στην παρούσα εργασία πραγματοποιήθηκε η γενοτύπηση ενενήντα ποικιλιών συκιάς (Ficus carica L.) που προέρχονται από τη λεκάνη της Μεσογείου και διατηρούνται σε Τράπεζα Γενετικού Υλικού ex situ. Συνολικά βρέθηκαν 91 αλληλόμορφα με μέσο όρο 13 αλληλόμορ...
Poster
Full-text available
Η πρόοδος στη βελτίωση των φυτών επικουρείται από ακριβείς πληροφορίες για τη γενετική ποικιλότητα. Στην παρούσα εργασία πραγματοποιήθηκε η γενοτύπηση ενενήντα ποικιλιών συκιάς (Ficus carica L.) που προέρχονται από τη λεκάνη της Μεσογείου και διατηρούνται σε Τράπεζα Γενετικού Υλικού ex situ. Συνολικά βρέθηκαν 91 αλληλόμορφα με μέσο όρο 13 αλληλόμορ...
Article
Full-text available
Barbary fig (Opuntia ficus indica) could be an economically important species as it could be an alternative crop extremely tolerant to dry condition and water deficiency. Moreover, it could be used in alternative sustainable cultivation systems and landscape conservation. In this work, we report the analysis of the genetic diversity of Greek Barbar...
Article
Full-text available
The conservation and characterization of summer squash (Cucurbita pepo) genetic resources in germplasm banks has been the basis of their use in breeding projects, which resulted in the development of new cultivars. The genetic diversity of thirty six summer squash landraces from Greece was investigated here using start codon targeted (SCoT) polymor...
Article
Full-text available
The causal agent of Fusarium wilt, Fusarium oxysporum Schlecht. f. sp. melongenae, produces a vascular disease of eggplant (Solanum melongena L.). Resistant germplasm with wide variation is essential for controlling this disease via breeding, and understanding the genetic background helps design breeding strategies. This study screened 36 S. melong...
Article
The conservation and characterization of eggplant (Solanum melongena L.) genetic resources in germplasm banks has been the basis of their use in breeding projects, which has resulted in the development of new cultivars. High Resolution Melting (HRM) analysis, combined with eight microsatellite markers, has been integrated in order to facilitate th...
Conference Paper
Η σύγχρονη γεωργία, οι συμβατικές μέθοδοι καλλιέργειας και η αλόγιστη χρήση των υψηλών εισροών έχει ως αποτέλεσμα την απώλεια της γενετικής ποικιλότητας και τη στασιμότητα των αποδόσεων ειδικά σε λιγότερο ευνοϊκές περιοχές. Ο χαρακτηρισμός της γενετικής ποικιλότητας των εγχώριων ποικιλιών μιας συλλογής γενετικού υλικού, με χρήση ουδέτερων και βασισ...
Article
Full-text available
Cucurbita pepo (squash, pumpkin, gourd), a worldwide cultivated vegetable of American origin, is extremely variable in fruit characteristics. Most of its widely grown commercial types are known as summer squashes and belong to the elongated forms of C. pepo ssp. pepo (Cocozelle, Vegetable marrow and Zucchini groups). Herein, we have integrated the...
Article
Full-text available
Identification of genotypes in Sideritis is complicated owing to the morphological similarity and common occurrence of natural hybridisation within Sideritis species. Species- and genotype-specific DNA markers are very useful for plant identification, breeding and preservation programs. Herein, a real-time polymerase chain reaction (PCR) of ITS2 ba...
Article
Full-text available
Olea europaea L. is cultivated in the Mediterranean region for thousands of years and is of major economic importance. Olive cultivars’ origin is perplexed as is their identification. Thus, their molecular characterization and discrimination will enable olive germplasm management. In addition, it would be a useful tool for olive product authenticat...
Article
Full-text available
Dactylis glomerata L. is an important forage species in the Mediterranean region and in regions having a similar climate. Genetic material from three locations covering the north, central and south Greece was studied, using morphological traits, SSRs and ISSRs molecular markers. Morphological analysis revealed differences among the studied geograph...
Article
Full-text available
Dactylis glomerata L. is an important forage species in the Mediterranean region and in regions having a similar climate. Genetic material from three locations covering the north, central and south Greece was studied, using morphological traits, SSRs and ISSRs molecular markers. Morphological analysis revealed differences among the studied geograph...
Article
Full-text available
Local breeds are important for the maintenance of genetic diversity and future food security. Nowadays the worldwide distribution of pigs is dominated by few breeds porting to a severe loss of pig biodiversity. Thus, it is critical to maintain distinct populations of pig breeds. Greek black pig, a breed raised locally for the high quality of meat f...
Data
(A) Genomic organization of CEN/TFL1-like gene and promoter analysis. Bindings elements are indicated boxed with different colors. Grey boxes with numbers inside indicate the four exons and their respective size. TSS: Transcription Start Site. (B) Promoter sequence. Predicted bindings sites are grey boxed. Putative TATA box in underlined. ATG start...
Article
Full-text available
Genes in the phosphatidyl-ethanolamine-binding protein (PEBP) family are instrumental in regulating the fate of meristems and flowering time. To investigate the role of these genes in the monocotyledonous plant Crocus (Crocus sativus L), an industrially important crop cultivated for its nutritional and medicinal properties, we have cloned and chara...
Article
Full-text available
Wild cherry (Prunus avium L.) is a widespread, partially asexual, noble hardwood European species characterized by a scattered distribution, small population sizes, and human exploitation for its valuable wood. These characteristics, especially at the southern limits of the species natural distribution where additional varying stresses may occur, r...
Article
The actin-binding protein profilin (PRF) plays an important role in cell growth and expansion by regulating the organization of the actin filaments. Recent studies have reported association between fiber elongation in cultivated cotton (Gossypium hirsutum) and PRF expression. In the present study, we cloned four genomic clones from allotetraploid c...
Article
Full-text available
Cotton is the most important textile plant in the world and is one of the most important crops for the production of oilseed. Because of its worldwide economic importance, new cultivars are constantly being released in the world and consequently in the Greek market, as Greece is the largest producer in Europe. We used simple sequence repeat (SSR) m...
Article
Full-text available
In Arabidopsis, it has been shown that the B-class MADS-box genes are expressed in the developing whorl 2 petals and whorl 3 stamens throughout the ontogeny of these organs. APETALA3/PISTILLATA (AP3/PI) heterodimers act as an inducer of a regulator-coding gene called NAP, a homologue to NAC-like transcription factor genes, required for meristem est...
Article
Full-text available
We describe an improvement of the RCA-RACE (rolling circle amplification-rapid amplification of cDNA ends) method, called family RCA-RACE (famRCA-RACE). The method is based on the generation of circular cDNA fragments, followed by rolling circle amplification of the circular cDNA using phi29 DNA polymerase and the application of PCR using degenerat...
Chapter
Flowering and flower formation is a major developmental switch in plants where different genes are expressed in a coordinate manner for reproductive success. In order to uncover and understand the molecular mechanisms controlling flowering and flower formation in cultivated Crocus sativus L. and to characterize a number of crocus flower mutants, we...
Chapter
Flowering and flower formation is a major developmental switch in plants where different genes are expressed in a coordinate manner for reproductive success. Among these genes MADS domain transcription factors and FLOWER LOCUS T like genes play important roles in various developmental processes in flowering plants, from transition to flowering to t...
Article
For uncovering and understanding the molecular mechanisms controlling flower development in cultivated Crocus sativus and particularly the transformation of sepals in outer whorl (whorl 1) tepals, we have cloned and characterized the expression of a family of five PISTILLATA/GLOBOSA-like (PI/GLO-like) MADS-box genes expressed in the C. sativus flow...