Anurup Mohanty

Anurup Mohanty
Indian Institute of Science | IISC · Department of Biochemistry

Bachelor of Technology
I am an early career researcher exploring astrobiology using Petri plates, microscopes and computers.

About

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Introduction
I'm a biotechnology student passionate about Astrobiology. My current research is focused on extremophilic microbes and tardigrades.
Additional affiliations
November 2020 - present
Blue Marble Space Institute of Science
Position
  • Visiting Scholar
June 2020 - October 2020
Blue Marble Space Institute of Science
Position
  • Research Associate
Description
  • BMSIS Young Scientist Program 2020 : An Internship with the Center for Life Detection Science, NASA Ames Research Center. Mentors : Dr. Andro C Rios & Dr. Graham Lau
Education
July 2018 - July 2022
SRM Institute of Science and Technology
Field of study
  • Biotechnology

Publications

Publication (1)
Article
Astrobiology is an emerging and interdisciplinary scientific field that aims at studying and understanding life in the universe, with research topics including the origin of life and the feasibility of life existing and being detected elsewhere in the universe. In this article, we highlight the critical role that astrobiology plays toward inspiring...

Questions

Questions (13)
Question
I'm looking for some resources (article or videos or courses) to understand the working of Illumina and other NGS platforms? I have a lot of concept gaps and doubts.
Question
We have collected some soil samples from a pond. We want to do bacterial cultivation-based work in the lab. What is the best way to store the samples for a few months?
Question
Say I have a bacterial genome, and I have located the CRISPR and spacer regions in the genome. Is there any way to find out the phage(s) that infected this bacteria by using those spacers?
Would you please recommend databases and tools to do the same?
Question
What are some methods that can study the transfer of genes laterally in different species of microbes under lab conditions. I'm mainly looking for cost-effective ways.
Question
I'm working on isolation of bacteria that can dissolve calcium carbonate.
The isolated bacteria produced a zone of clearance in agar plate containing CaCO3 (indicating the dissolution).
I'm looking for a simple method to do the same (but quantitatively) in broth.
PS: CaCO3 doesn't dissolve in water/medium, hence settles down.
Question
I went through some literature to understand the basic concepts behind the Sanger - Chain Termination Method of sequencing. I have understood the concepts, but I have a doubt. The Sanger sequencing method (invented in 1977) uses PCR, developed in 1985. Am I missing something?
  • Did the original sanger protocol use some other method?
Question
I have noticed this gauge in all the cabinets at my institution (not sure if it's that). I can't think of any use for having it. Can someone please explain?
Question
We are trying to culture thermophiles in our lab.
The samples were obtained from sediments and biofilms near a hot spring; we noticed significant turbidity in the enrichment flasks after incubation at 60°C for 2 days. The enriched media was used as an inoculum for agar plates but we noticed that:
  • The parafilm became perforated,
  • The agar started drying up and cracks showed up,
  • No colonies appeared even after 3 days of incubation at 60°C
Can someone suggest ways to prevent this from happening? Any other tips related to culturing of thermophiles for beginners are welcome.
Question
Hypothetical Question:
If all the microorganisms in a food item are removed/killed, will it stay fresh* for an indefinite amount of time?
*I mean edible after a long period of time.
Question
We find dinosaur fossils after digging deep in the soil and if I'm not wrong the stratigraphic arrangement of soil is caused due to weathering of mountains.
Will a time come when there are no more mountains to weather? (Or do they keep forming)
Will some of us (present-day humans/animals) get fossilized or have advancements in science and technology changed this?
Question
I'm trying to study the effects of desiccation on some proteins, could someone tell what solvent or what simulation conditions I should set up to simulate a desiccation environment on GROMACS.
Question
I'm an undergraduate and looking for an introductory book that dives into molecular mechanisms used by extremophiles and some culturing methods.
Question
I have an amino acid sequence
  • If I run it on NCBI tblastn I get the expected results.
But If I take the same amino acid sequence
Don't know why this is happening.