Antonio Palazzo

Antonio Palazzo
Università degli Studi di Bari Aldo Moro | Università di Bari · Department of Biology

PhD

About

49
Publications
3,794
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198
Citations
Citations since 2017
27 Research Items
192 Citations
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20172018201920202021202220230102030405060
20172018201920202021202220230102030405060
20172018201920202021202220230102030405060

Publications

Publications (49)
Article
Full-text available
We here describe a leukemogenic role of the homeobox gene UNCX, activated by epigenetic modifications in acute myeloid leukemia. We found the ectopic activation of UNCX in a leukemia patient harboring a t(7;10)(p22;p14) translocation, in 22/61 of additional cases [a total of 23 positive patients out of 62 (37.1%)], and in 6/75 (8%) of acute myeloid...
Article
Full-text available
Bari elements are members of the Tc1-mariner superfamily of DNA transposons, originally discovered in Drosophila melanogaster, and subsequently identified in silico in 11 sequenced Drosophila genomes and as experimentally isolated in four non-sequenced Drosophila species. Bari-like elements have been also studied for their mobility both in vivo and...
Article
Full-text available
Background Bari-like transposons belong to the Tc1-mariner superfamily, and they have been identified in several genomes of the Drosophila genus. This transposon’s family has been used as paradigm to investigate the complex dynamics underlying the persistence and structural evolution of transposable elements (TEs) within a genome. Three structural...
Article
Full-text available
The transposons of the Bari family are mobile genetic elements widespread in the Drosophila genus. However, despite a broad diffusion, virtually no information is available on the mechanisms underlying their mobility. In this paper we report the functional characterization of the Bari elements transposition system. Using the Bari1 element as a mode...
Article
The exact mechanism by which ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1) inhibits insulin signaling is not known. ENPP1 contains two somatomedin-B-like domains (i.e. SMB 1 and 2) involved in ENPP1 dimerization in animal cells. The aim of the present study was to investigate if these domains modulate ENPP1 inhibitory activity on insul...
Article
Small Cell Lung Cancer (SCLC) is treated as a homogeneous disease, although the expression of NEUROD1, ASCL1, POU2F3, and YAP1 identifies distinct molecular subtypes. The MYC oncogene, amplified in SCLC, was recently shown to act as a lineage-specific factor to associate subtypes with histological classes. Indeed, MYC-driven SCLCs show a distinct m...
Article
Full-text available
The piggyBac DNA transposon is an active element initially isolated from the cabbage looper moth, but members of this superfamily are also present in most eukaryotic evolutionary lineages. The functionally important regions of the transposase are well described. There is an RNase H-like fold containing the DDD motif responsible for the catalytic DN...
Article
Full-text available
The maintenance of genome integrity is ensured by proper chromosome inheritance during mitotic and meiotic cell divisions. The chromosomal counterpart responsible for chromosome segregation to daughter cells is the centromere, at which the spindle apparatus attaches through the kinetochore. Although all mammalian centromeres are primarily composed...
Article
Full-text available
Background: Neuroendocrine tumors (NETs) overexpress somatostatin receptors (SSTRs). Methods: We developed a second-generation, ligand-based, anti-SSTR chimeric antigen receptor (CAR) incorporating the somatostatin analog octreotide in its extracellular moiety. Results: Anti-SSTR CAR T cells exerted antitumor activity against SSTR+NET cell lin...
Article
Full-text available
Transposable elements (TEs) have been historically depicted as detrimental genetic entities that selfishly aim at perpetuating themselves, invading genomes, and destroying genes. Scientists often co-opt “special” TEs to develop new and powerful genetic tools, that will hopefully aid in changing the future of the human being. However, many TEs are g...
Article
Full-text available
Chromatin is a highly dynamic biological entity that allows for both the control of gene expression and the stabilization of chromosomal domains. Given the high degree of plasticity observed in model and non-model organisms, it is not surprising that new chromatin components are frequently described. In this work, we tested the hypothesis that the...
Article
Full-text available
The role of extracellular vesicles (EVs) has been completely re-evaluated in the recent decades, and EVs are currently considered to be among the main players in intercellular communication. Beyond their functional aspects, there is strong interest in the development of faster and less expensive isolation protocols that are as reliable for post-iso...
Article
Full-text available
Advanced colorectal cancer (CRC) is highly metastatic and often results in peritoneal dissemination. The extracellular vesicles (EVs) released by cancer cells in the microenvironment are important mediators of tumor metastasis. We investigated the contribution of EV-mediated interaction between peritoneal mesothelial cells (MCs) and CRC cells in ge...
Article
Expression vectors (EVs) are artificial nucleic acid molecules with a modular structure that allows for the transcription of DNA sequences of interest in either cellular or cell-free environments. These vectors have emerged as cross-disciplinary tools with multiple applications in an expanding Life Sciences market. The cis-regulatory sequences (CRS...
Article
Full-text available
Prostate cancer is one of the most common malignancies in men. It is characterized by a high molecular genomic heterogeneity and, thus, molecular subtypes, that, to date, have not been used in clinical practice. In the present paper, we aimed to better stratify prostate cancer patients through the selection of robust long non-coding RNAs. To fulfil...
Article
The number of reports concerning horizontal transposon transfers (HTT) in metazoan species is considerably increased, alongside with the exponential growth of genomic sequence data However, our understanding of the mechanisms of such phenomenon is still at an early stage. Nematodes constitute an animal phylum successfully adapted to almost every ec...
Article
Full-text available
Reproducibility of results is essential for a well-designed and conducted experiment. Several reasons may originate failure in reproducing data, such as selective reporting, low statistical power, or poor analysis. In this study, we used PEG6000 samples from different distributors and tested their capability inducing spheroid formation upon surface...
Article
Full-text available
Transposable elements (TEs) are constitutive components of both eukaryotic and prokaryotic genomes. The role of TEs in the evolution of genes and genomes has been widely assessed over the past years in a variety of model and non-model organisms. Drosophila is undoubtedly among the most powerful model organisms used for the purpose of studying the r...
Article
Full-text available
The Sleeping Beauty (SB) transposon is an advanced tool for genetic engineering and a useful model to investigate cut-and-paste DNA transposition in vertebrate cells. Here, we identify novel SB transposase mutants that display efficient and canonical excision but practically unmeasurable genomic re-integration. Based on phylogenetic analyses, we es...
Preprint
Full-text available
In this study we report about the reproducibility of three-dimensional cell culture of floating cell spheroids on PEG6000 treated cell culture dishes. Three-dimensional tumour spheroids or organoids present an interesting test platform for nanoparticulated drug delivery or nanoparticle toxicity. We tested the reproducibility of spheroid formation i...
Article
Full-text available
Background We have recently described a peculiar feature of the promoters in two Drosophila Tc1-like elements, Bari1 and Bari3. The AT-richness and the presence of weak core-promoter motifs make these promoters, that we have defined “blurry”, able to activate transcription of a reporter gene in cellular systems as diverse as fly, human, yeast and b...
Article
Full-text available
The contribution of the transposons’ promoter in the horizontal transfer process is quite overlooked in the scientific literature. To shed light on this aspect we have mimicked the horizontal transfer process in laboratory and assayed in a wide range of hosts (fly, human, yeast and bacteria) the promoter activity of the 5′ terminal sequences in Bar...
Data
Transposons co-occurrence near the insertion site of Bari elements in single species. The red-boxed area in each plot indicates area of significant (p<0,05) enrichment (positive values) or depletion (negative values) of the analyzed transposable elements’ super-families in the proximity of Bari elements in a sequence range of 2,5 kb. X axes report...
Data
WebLogo analysis. Fifty bp upstream or downstream the Bari elements in 8 Drosophila species were analyzed. The number of sequences analyzed is reported (n). (TIF)
Data
Complete dataset and features of Bari-related MITEs annotated in each species analyzed. (XLSX)
Data
Tukey test and Kruskal-Wallis test results. (PDF)
Data
P-distance analyses and deterioration profiles of Bari elements in all the analyzed species. (TIF)
Data
Detection of a recombination site generating a deletion in the heterochromatic cluster. A. Possible molecular mechanism generating the observed adjacent heterochromatic copies carrying deletions of terminal sequences. (B) Global alignment (Needleman-Wunsch) of two adjacent canonical Bari1 heterochromatic copies and the detected copies carrying a de...
Data
Multiple alignment of representative MITEs and reference Bari elements. (PDF)
Data
Accession numbers and coordinates of 1000 randomly selected sequence used as control dataset in flanking sequences analysis. (XLSX)
Data
Genome assemblies used for analyses. (PDF)
Data
Complete dataset and features of Bari elements annotated in each species analyzed. (XLSX)
Article
We describe a new AML entity, occurring in 30% of de novo acute myeloid leukemia, due to structural and epigenetic deregulation of the UNCX homeobox (HB) gene. By molecular approaches, we identified a M5 AML patient with a t(7;10)(p22;p14) translocation as the sole cytogenetic anomaly and showing ectopic expression of UNCX (7p22.3), which encode fo...
Patent
Full-text available
The invention refers to 5'TIR nucleotide sequences derived from the "Bari" family of transposable elements for the use as promoter in the expression of a transgene in a recombinant genetic construct adapted to be transfected in a host cell system.

Questions

Question (1)
Question
Hi,
I'm setting a good protocol for exosome isolation by cellular conditionate medium with Fixed angle BECKMAN COULTER ultracentrifuge. To date I'm working with polypropylene tubes but I don't like that tube becouse they haven't any cap and it does not allow me to see any pellets.
What is your experience in this particular area?

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Projects

Project (1)
Project
Understand a little piece of the evolutionary history of mobile elements and how they survived in extant genomes, with special attention to horizontal transposon transfer. Develop molecular genetics tools from transposable elements or part of them.