Antonio Moreda-Piñeiro

Antonio Moreda-Piñeiro
University of Santiago de Compostela | USC · Faculty of Chemistry

PhD

About

205
Publications
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Publications

Publications (205)
Article
Intensive production of nanomaterials, especially metallic nanoparticles (MNPs), and their release into the environment pose several risks for humans and ecosystem health. Consequently, high-efficiency analytical methodologies are required for control and characterization of these emerging pollutants. Single-particle inductively coupled plasma – ma...
Article
Seaweed can bioaccumulate nanomaterials that would be transferred to the trophic chain. This work describes the optimization of a method for the separation of silver nanoparticles (AgNPs) from seaweed using an ultrasound-assisted enzymatic hydrolysis method and ulterior determination by single particle inductively coupled plasma mass spectrometry (...
Article
The use of zinc oxide (ZnONPs) and titanium dioxide nanoparticles (TiO2NPs) in cosmetic formulations to obtain protection against the sun's UV radiation has become a common practice owing to their high photo-stability and the absence of allergic reactions of these nanomaterials. The manufacturing of cosmetics modified with nanoparticles has been re...
Article
In recent decades, the remarkable thermal, electronic, optical and mechanical properties of smart materials have attracted the attention of analytical chemists intending to address problems found in metal determination in food (use of time-consuming procedures and expensive techniques to reach the low limits of detection required for quality contro...
Article
The present study aims to give information regarding the quantification of ZnO-NPs in canned seafood, which may be intentionally or unintentionally added, and to provide a first esteem of dietary exposure. Samples were subjected to an alkaline digestion and assessment of ZnO-NPs was performed by the single particle ICP-MS technique. ZnO-NPs were fo...
Article
Full-text available
Patients with rheumatoid arthritis (RA) show autoantibodies against post-translational protein modifications (PTMs), such as anti-citrullinated protein antibodies. However, the range of recognized PTMs is unknown. Here, we addressed four PTMs: chlorination, non-enzymatic glycation, nitration, and homocysteinylation, identified as targets of atypica...
Article
Full-text available
Metals, metallic compounds, and, recently, metallic nanoparticles appear in textiles due to impurities from raw materials, contamination during the manufacturing process, and/or their deliberate addition. However, the presence of lead, cadmium, chromium (VI), arsenic, mercury, and dioctyltin in textile products is regulated in Europe (Regulation 19...
Article
Analytical methods based on dynamic-reaction cell (DRC) technology using ammonia as a reaction gas have been developed for the determination of ultra-trace Ti, Zn, Cu and Ag by inductively coupled plasma mass spectrometry (ICP-MS). Challenging spectral interferences from complex matrices were demonstrated to be overcome by DRC, and several DRC appr...
Article
The European Food Safety Authority has published a guidance regarding risk assessment of nanomaterials in food and feed. Following these recommendations, an in vitro gastrointestinal digestion has been applied to study the biopersistence of TiO2 and Ag NPs in standards, molluscs and surimi. TiO2 NPs standards and TiO2 NPs/ TiO2 microparticles from...
Article
Ultrasound-Assisted Extraction (UAE) has attracted much attention in recent years due to its rapid extraction capability, high extraction efficiency, and being an environmentally friendly and low-cost methodology. This review gives a general overview of UAE and specifically its recent applications on fish and aquatic living resources. Moreover, the...
Article
In this study, a first attempt for isolating and determining (characterising) background levels of titanium dioxide nanoparticles (TiO2 NPs) in seaweed has been developed by using single particle inductively coupled plasma – mass spectrometry (SP-ICP-MS). Seaweeds were processed using an optimised ultrasound assisted extraction (UAE) procedure base...
Chapter
Molecularly imprinted technology (MIT) consists of preparing materials exhibiting specific recognition cavities to selective mimic the target analytes. The prepared materials promote selective interactions with the targets and avoid interactions of concomitants from complex food, biological, clinical, and environmental matrices. This chapter provid...
Article
A selective molecularly imprinted polymer (MIP) adsorbent was synthesised and used in a batch micro-solid phase extraction format for isolating aflatoxins (AFB1, and AFB2) from non-dairy beverages before liquid chromatography-tandem mass spectrometry determination. MIP synthesis (precipitation polymerization in 3 : 1 acetonitrile/toluene as a porog...
Article
Full-text available
The review describes the development of batch solid phase extraction procedures based on dispersive (micro)solid phase extraction with molecularly imprinted polymers (MIPs) and magnetic MIPs (MMIPs). Advantages and disadvantages of the various MIPs for dispersive solid phase extraction and dispersive (micro)solid phase extraction are discussed. In...
Article
A method based on asymmetric flow field-flow fractionation (AF4) coupled to ultraviolet-visible (UV-vis) spectroscopy and inductively coupled plasma mass spectrometry (ICP-MS) has been developed for silver nanoparticles (Ag NPs) detection and quantification in bivalve molluscs. Samples were pre-treated using a conventional enzymatic (pancreatin and...
Article
Titanium dioxide nanoparticles (TiO2 NPs) are widely used in industry as a white pigment (paints, paper industry and toothpastes), photocatalysts (environmental decontamination and photovoltaic cells), inorganic UV filter (sunscreens and personal care products) and as a food additive (E171) and antimicrobial food packaging material. Silver nanopart...
Article
The use of magnetic nanoparticles (MNPs) as adsorbent/separation agents for the removal of heavy metal-based compounds from aqueous environments has received much attention in the last few years. However, in most cases, the designed particles present large dimensions (at least several tens of nm) in order to provide them with high magnetic energy a...
Article
Trace elements in dried blood spots (DBS) from new-born were determined by laser ablation (LA) coupled with inductively coupled mass spectrometry (ICP-MS), and data was subjected to chemometric evaluation in an attempt to classify healthy new-born and new-born suffering metabolic disorders. Unsupervised [principal component analysis (PCA), and clus...
Chapter
This chapter discusses in detail the potential of several combined extraction and microextraction techniques for pretreating food waste. The discussion is focused on the combination of well-established methodologies such as ultrasound/microwave-assisted extraction and microwave-assisted acid digestion used for target extraction with preconcentratio...
Article
In contrast to most of essential and heavy metals, mercury levels in seaweed are very low, and pre-concentration methods are required for an adequate total mercury determination and mercury speciation in this foodstuff. An ionic imprinted polymer-based solid phase extraction (on column) pre-concentration procedure has been optimized for mercury spe...
Article
A dispersive micro solid phase extraction approach using a molecularly imprinted polymer as an adsorbent has been developed for pre-concentrating aflatoxins from cultured fish. Aflatoxins were first isolated from fish muscle and liver by an ultrasound assisted extraction procedure using a 60:40 acetonitrile/0.1M KH2PO4 aqueous buffer (pH 6.0) mixtu...
Article
This study combines ultrasound-assisted extraction and vortex-assisted dispersive micro-solid phase extraction using an ionic imprinted polymer as a selective sorbent for rapid isolation and pre-concentration of inorganic arsenic species (As(III) and As(V)) in extracts from rice samples prior to their determination by high performance liquid chroma...
Article
A procedure for the size characterization and quantification of titanium dioxide (TiO2) nano- and microparticles by Asymmetric Flow Field-Flow Fractionation (AF4) coupled to Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) is described. Different strategies for size characterization with size standards and th...
Article
Non-essential trace elements, especially mercury (Hg) and arsenic (As), and their speciation analysis in fish and fishery products have gained increasing attention regarding human exposure, risk assessment and toxicological endpoints. Reliable, robust and sophisticated hyphenated laboratory instrumentation is available today, providing very low qua...
Article
An analytical procedure using low-cost instrumentation (fluorescence/phosphorescence spectrophotometer) has been developed to assess total mercury in fishery products. Determinations were based on the room temperature phosphorescence (RTP) quenching of a composite Ph-QDs consisting of phenobarbital-containing polymer/silica coated Mn-doped ZnS quan...
Article
Low levels of inorganic arsenic [As(III) and As(V)] in fishery products have been selectively isolated from fish extracts (1.0 g of wet fish samples pre-treated with 10 mL of 1:1 methanol/water under sonication at 25 °C for 30 min) by ionic imprinted polymer (IIPs) based solid phase extraction procedure (on-column mode). The selective adsorbent was...
Article
A novel room temperature phosphorescence chemosensor probe has been successfully developed and applied to the selective detection and quantification of inorganic arsenic (As(III) plus As(V)) in fish samples. The prepared material (IIP@ZnS:Mn QDs) was based on Mn-doped ZnS quantum dots coated with (3-aminopropyl) triethoxysilane and an As(III) ionic...
Article
Despite aflatoxins contamination in feedstuff is a well‐known problem, and hence these residues are controlled in poultry products, there are scarce information regarding the presence of these toxic substances in aquaculture fish, facilities which use several feedstuff for fish breeding. A simple, rapid, and sensitive method has been therefore deve...
Article
Possibilities of room temperature spectrometry based on Mn-doped ZnS quantum dots coated with a molecularly imprinted polymer based nanosensor have been explored for the sensitive and selective determination of aflatoxins. Synthesized polymeric nanoparticles exhibit intense room temperature phosphorescence (total decay time of 0.004 s) and aflatoxi...
Article
A combined procedure based on using ultrasounds for target isolation followed by porous membrane-protected micro solid phase extraction using a molecularly imprinted polymer as an adsorbent has been developed as a highly selective extraction and clean-up procedure for isolating aflatoxins B1, B2, G1, and G2 from fish feed before ultra-high-performa...
Article
Advances on nanometrology require reliable sample pre-treatment methods for extracting/isolating nanomaterials from complex samples. The current development deals with a discontinuous ultrasonication (60% amplitude, 15 cycles of ultrasound treatment for 59 s plus relaxing stage for 59 s, 20 mL of methanol) method for a fast and quantitative extract...
Article
A method based on single-particle inductively coupled plasma mass spectrometry (sp-ICPMS)using a dwell time 50 μs for acquisition has been developed for silver nanoparticles (Ag NPs)determination and characterization in bivalve molluscs. Ultrasound assisted enzymatic hydrolysis has been proposed for a fast isolation of Ag NPs from seafood using pan...
Article
This review highlights the increasing interest and application of combining sorbent enrichment, liquid-phase, and membrane-based microextraction techniques in Analytical Chemistry and its sustainable green applications to efficient target analyte extraction and pre-concentration/clean-up from foodstuff. Several combinings involving microwave/ultras...
Article
Synthesis of molecularly imprinted polymers (MIPs) implies a previous pre-polymerization stage in which the template (the molecule for which the MIP offers the specific recognition cavities) interacts with the adequate functional monomer, just before the addition of the cross-linker and the initiator for performing the polymerization synthesis. The...
Article
Discrete sampling based on the flow injection options offered by advanced autosamplers has been tested and applied for the direct analysis of low volume samples (human serum) for multi-elemental purposes (simultaneous Al, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Ni, Pb, Rb, Se, V, and Zn assessment). Serum samples (200 µL aliquots) were direc...
Article
Synthesized molecularly imprinted polymer (MIP) materials have been anchored on the surface of PEG-Mn-doped ZnS quantum dots (QDs) to develop a fluorescence probe for aflatoxins (AFs) recognition/determination in non-dairy beverages. MIP synthesis used 5,7-dimethoxycoumarin (DMC) as a dummy template molecule, and methacrylic acid (MAA) as a functio...
Article
Full-text available
A reliable method based on high-performance liquid chromatography-tandem mass spectrometry has been developed for the assessment of coca alkaloids/metabolites [cocaine (COC), benzoylecgonine (BE), cocaethylene (CE), ecgonine methyl ester (EME), anhydroecgonine methyl ester (AEME), tropococaine (TRO), transcinnamoylcocaine (trCIN), cuscohygrine (CUS...
Article
A reliable sample pre-treatment based on enzymatic hydrolysis has been fully optimized and validated for TiO2 NPs isolation and determination/characterization in surimi (crab sticks). Efficient extractions have been found when using a pancreatin/lipase mixture at pH 7.4 and 37 °C for 12 h under continuous stirring. The proposed sample pre-treatment...
Article
Size exclusion chromatography (SEC) hyphenated to inductively coupled plasma – mass spectrometry (ICP-MS), as a specific detector for metals, has been used for monitoring and determining metal-low molecular weight organic compound (LMWC) complexes in natural wines. SEC with UV detection (wavelength of 205 nm) was used for monitoring organic compoun...
Article
Bioavailability of elements in ‘natural’ wines from Chile’s Itata Valley has been assessed using an in vitro dialyzability approach. The red wines (fifteen samples) were of the Cinsault, Cabernet Sauvignon, Carmènére, Malbe, and Pinot noir varieties. All white wines (three samples) were of the Muscat of Alexandria variety. Inductively coupled plasm...
Article
Synthetic cathinones are a type of drugs belonging to group of "new psychoactive substances" (NPSs). The illicit market of these substances is characterized by the continuous introduction in the market of new analogs to evade legislation and to avoid be detected. New screening and confirmation assays are therefore needed, mainly in forensic/clinica...
Article
A novel approach for serum analysis by dried matrix spot (DMS) technique is proposed. The methodology consists of sampling filter paper discs (2.7 mm in diameter) containing the large amount of serum retained after a single spotting. Several oxidizers (sodium chlorate, sodium azide, acetic acid, formic acid, 1-butyl-3-methylimidazoliumm chloride/br...
Article
Applicability of single-particle inductively coupled plasma mass spectrometry (sp-ICP-MS) using dwell times equal to or shorter than 100 μs has been tested for assessing titanium dioxide nanoparticles (TiO2 NPs) in bivalve mollusks. TiO2 NPs isolation from fresh mollusk tissues was achieved by ultrasound assisted enzymatic hydrolysis procedure usin...
Article
Several molecularly imprinted polymers (MIPs) have been synthesized for the first time using various synthetic cannabinoids (JWH007, JWH015 and JWH098) as template molecules. Ethylene dimethacrylate (EDMA) was used as a functional monomer for all cases. Similarly, divinylbenzene (DVB) and 2,2'-azobisisobutyronitrile (AIBN) were used as cross-linker...
Article
An in vitro dialyzability approach has been undertaken to elucidate the bioavailable fraction of the total polyphenols (TPs) of edible nuts and seeds. The TP contents in samples and in dialyzates were assessed by the Folin-Ciocalteu spectrophotometric method. Antioxidant activity was determined in selected samples, using a modified method against T...
Article
A molecularly imprinted polymer – Mn-doped ZnS quantum dot-based fluorescence probe for cocaine abuse screening has been prepared and applied to complex samples such as serum and oral fluid. The fluorescent sensing material was prepared by anchoring a selective MIP for COC on the surface of polyethylene glycol (PEG) modified Mn-doped ZnS quantum do...
Article
Background: This review describes the use of molecularly imprinted polymers (MIPs) as tailor-made adsorbents for developing micro-solid phase extraction (µ-SPE) procedures when isolat-ing/assessing organic pollutants, drugs, and bioactive compounds from environmental, food, and clini-cal/forensic samples. Objective: Developments in MIP-µ-SPE are fo...
Article
An approach by laser ablation (LA) coupled with inductively coupled mass spectrometry (ICP-MS) for multi-element determination in whole blood is described. The method allows quantitative determination of several major (K, Na and S), minor (Ca, Cu, Fe, Mg, P, Rb and Zn), and trace elements (Al, As, Ba, I, Li, Pb, Sb, V and Tl) in dried blood spot (D...
Chapter
Current trends in the analytical laboratory imply the development of rapid, simple and reduced solvent consumption techniques for sample preparation as a previous stage before target determination. Sorbent and solvent microextraction techniques have been intensively applied as sample pretreatment tools for this purpose when treating a wide range of...
Article
Full-text available
A molecularly imprinted polymer (MIP) selective for cannabinoids [Δ(9)-tetrahydrocannabinol (Δ9-THC), 11-nor-9-carboxy-Δ(9)-tetrahydrocannabinol (Δ9-THC-COOH), and 11-hydroxy-Δ(9)-tetrahydrocannabinol (Δ9-THC-OH)] has been synthesized, fully characterized, and applied to the assessment of plasma and urine analysis of marijuana abuse by high perform...
Article
Metalloproteins and metal–protein complexes play key roles in all organisms. For example, certain metalloproteins are involved in metal homeostasis and detoxification, or oxidative stress protection; whereas, metals serve as essential cofactors in a large number of metal–protein complexes. Advances in analytical instrumentation as well as informati...
Article
A simple sample pre-treatment method based on solid phase microextraction (SPME) and gas chromatography–mass spectrometry (GC-MS) has been optimized and validated for the assessment of 15 residual solvents (2-propanol, 2-methylpentane, 3-methylpentane, acetone, ethyl acetate, benzene, hexane, methylcyclohexane, methylcyclopentane, m-xylene, propyl...
Article
A selective molecularly imprinted polymer synthesized for the selective retention of cocaine (COC) and its metabolites [benzoylecgonine (BZE), ecgonine methyl ester (EME), and cocaethylene (CE)] was used as a solid adsorbent for assessing cocaine abuse by plasma analysis. The MIP beads (50 mg) were loaded inside a cone shaped device made of a polyp...
Article
Bioavailability of essential and toxic metals in edible nuts and seeds has been assessed by using an in vitro dialyzability approach. The samples studied included walnuts, Brazil nuts, Macadamia nuts, pecans, hazelnuts, chestnuts, cashews, peanuts, pistachios and seeds (almond, pine, pumpkin and sunflower). Metals were measured by inductively coupl...
Article
A novel adsorbent, magnetic molecularly imprinted polymer (MMIP) based on magnetite nanoparticles surface-covered with an MIP selective for cocaine (COC), was synthesised and used in batch micro-solid phase extraction (μ-SPE) for isolation of COC and metabolites (benzoylecgonine, BZE; cocaethylene, CE; and ecgonine methyl ester, EME) from plasma sa...
Article
A new molecularly imprinted polymer (MIP)-based fluorescent artificial receptor has been prepared by anchoring a selective MIP for cocaine (COC) on the surface of polyethylene glycol (PEG) modified Mn-doped ZnS quantum dots (QDs). The prepared material combines the high selectivity attributed to MIPs and the sensitive fluorescent property of the Mn...
Article
Full-text available
Arsenic (As) speciation in human urine was performed by anion exchange chromatography (AEC), followed by inductively coupled plasma mass spectrome- try detection (ICP-MS). Separa- tion of arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid [MMA(V)], dimethylarsinic acid [DMA(V], arsenobetaine (AsB), and arsenocholine (AsC) in urine samples...
Research
Full-text available
El objetivo de este trabajo es el desarrollo de sensores fluorescentes para cocaína y sus metabolitos empleando nanopartículas de ZnS-Mn (quantum dots: QDs) recubiertos de polímeros de impronta molecular (MIP) selectivos a la droga. En primer lugar se realiza la síntesis de las nanopartículas y del MIP sobre las mismas. A continuación se optimizan...
Research
Full-text available
A novel method has been developed for cocaine and metabolites (benzoylecgonine, BEC; and ecgonine methyl ester, EME) assessment based on the quenching of fluorescence emission from Mn-doped ZnS quantum dots (QDs) – molecularly imprinted polymer (MIP) by cocaine and analogues. Mn-doped ZnS QDs were synthesized in inert atmosphere followed by polyeth...
Article
A magnetic molecularly imprinted polymer (MMIP) has been synthesized and applied for cocaine (COC) and metabolites (benzoylecgonine, BZE; cocaethylene, CE; and ecgonine methyl ester, EME) recognition/pre-concentration in urine samples. The MMIP has been prepared using COC as a template molecule, ethylene dimethacrylate (EDMA) as a functional monome...
Article
Porous membrane-protected micro-solid phase extraction (mu-SPE) using a molecularly imprinted polymer (MIP) as an adsorbent has been proposed as an integrated extraction-cleanup procedure for isolating cocaine (COC) and its metabolites [benzoylecgonine (BZE), ecgonine methyl ester (EME), and cocaethylene (CE)] from human urine. MIP beads have been...
Article
Dissolved proteins characterization in marine sediment pore water has been performed by offgel electrophoresis (OGE) and microfluidic lab-on-chip (LOC) electrophoresis, assessing for the first time dissolved protein characteristics such as isoelectric point (pIs), and molecular weight (MW). In addition, speciation studies of metal-protein complexes...
Article
Mn-doped ZnS quantum dots (QDs) coated with a molecularly imprinted polymer (MIP) material selective toward cocaine and its metabolites have been prepared and applied to cocaine (COC) and metabolites assessment by spectrofluorimetry. Ultrasound irradiation (37kHz) was novelty used for performing the Mn-doped ZnS QDs synthesis as well as for prepari...
Article
Sorbent and solvent microextraction techniques, mainly solid-phase microextraction, stir-bar sorptive extraction, single-drop microextraction, dispersive liquid–liquid microextraction, and hollow-fiber liquid-phase microextraction, are well-established, environment-friendly procedures for treating a wide range of samples when isolating organic and...
Article
The assessment of selenium and selenium species bioavailability in foodstuff is of special concern on the context of human nutrition. In-vivo (human and animal), and in-vitro tests are important approaches for estimating the bioavailability of toxic and essential compounds to humans. An overview on in-vivo and in-vitro bioavailability assays for re...
Article
Two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D-SDS-PAGE) was applied to separate protein in dissolved organic matter (DOM) and in particulate organic matter (POM) from seawater. Dissolved proteins were concentrated and purified using tangential flow ultrafiltration (UF) and centrifugal ultrafiltration. Twelve proteins...
Article
Trace levels of inorganic mercury, methyl-mercury and ethyl-mercury have been assessed in seawater by high performance liquid chromatography (HPLC) hyphenated with inductively coupled plasma-mass spectrometry (ICP-MS) after solid phase extraction (SPE) pre-concentration with a novel synthesized ionic imprinted polymer. The adsorbent material was pr...
Chapter
The determination of toxic and essential (i.e. selenium species) compounds in food products and bioavailability studies of these compounds are of special concern in the context of human nutrition and human health risk assessment. In vivo and in vitro gastrointestinal extraction, also known as bioaccessibility/bioavailability/bioactivity, are import...
Chapter
Although in vivo human studies are the ultimate test to determine the bioavailability of an essential/toxic substance in foodstuffs, and that in vivo animal reflects the real situation; some of these experimental approaches are ethically controversial. As an alternative, bioavailability can also be estimated through various in vitro methods. An ove...
Article
Two-dimensional offgel and lab-on-a-chip (LOC) electrophoresis was performed for assessing proteins from marine plankton. Proteins were isolated from plankton samples by using a phenol/sodium dodecylsulfate (SDS) buffer (2:1) pH 8.0 mixture as an extracting solution. The SDS buffer consisted of 30% (m/v) sucrose, 2% (m/v) SDS, 0.1 M of Tris-HCl, pH...