Antonio Giordano

Antonio Giordano
Temple University | TU · Fels Institute for Cancer Research and Molecular Biology

M.D. Ph.D

About

75
Publications
43,718
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
7,177
Citations
Additional affiliations
May 2016 - present
Temple University
Position
  • Managing Director

Publications

Publications (75)
Article
Full-text available
More than 50% of all gynecologic tumors can be classified as rare (defined as an incidence of ≤6 per 100,000 women) and usually have a poor prognosis owing to delayed diagnosis and treatment. In contrast to almost all other common solid tumors, the treatment of rare gynecologic tumors (RGT) is often based on expert opinion, retrospective studies, o...
Article
Full-text available
Abstract: More than 50% of all gynecologic tumors can be classified as rare (defined as an incidence of ≤6 per 100,000 women) and usually have a poor prognosis owing to delayed diagnosis and treatment. In contrast to almost all other common solid tumors, the treatment of rare gynecologic tumors (RGT) is often based on retrospective studies, expert...
Article
Body mass index (BMI) is a main indicator of obesity and its association with breast cancer is well established. However, little is known in the metastatic setting, especially in HER2‐positive patients. We assessed the influence of BMI on clinical outcomes of patients treated with pertuzumab and/or trastuzumab emtansine (T‐DM1) for HER2+ metastatic...
Article
Full-text available
Nivolumab is a novel therapeutic option in NSCLC, associated with a significant survival gain compared with Docetaxel. However, predictive biomarkers are lacking. The presence of systemic inflammation has been correlated with poor outcome in many cancer types. We aimed to evaluate whether there is a correlation between some indicators of inflammati...
Article
Objectives: This review aims to provide a framework for evaluating the utility of virtual reality (VR) as a distraction intervention to alleviate pain and distress during medical procedures. We firstly describe the theoretical bases underlying the VR analgesic and anxiolytic effects and define the main factors contributing to its efficacy, which l...
Article
Full-text available
G protein-coupled receptors (GPCRs) are integral cell-surface proteins having a central role in tumor growth and metastasis. However, several GPCRs retain an atypical intracellular/nuclear location in various types of cancer. The pathological significance of this is currently unknown. Here we extend this observation by showing that the bradykinin B...
Chapter
Cancer is a disease that includes more than 200 variants each of which are characterized, at the molecular level, by a high heterogeneity owed to a myriad of genetic and epigenetic alterations. Tumorigenesis is a multistep process that drives normal cells to evolve progressively toward a malignant state, and ultimately to acquire metastatic feature...
Article
Autosomal recessive Pompe disease is a lysosomal disorder caused by mutations of the acid-α-glucosidase (GAA) gene. Deficiency of GAA enzyme leads to glycogen accumulation and autophagy impairment in cardiac and skeletal muscles, but also in lymphocytes. Since an effective therapy is available, a rapid, sensitive and specific test is crucial to ear...
Chapter
In recent years the treatment of cancer patients has profoundly changed due to a better comprehension of the biological processes underlying tumor development and progression. Several tumors are defined as “oncogene addicted” and this discovery has led the way to the development of target therapies that are able to specifically kill cancer cells sp...
Article
The study of the physiological action of microorganisms in artistic materials is one of the most interesting topics in biodeterioration nowadays. Pathologies and illnesses of organic and inorganic materials provoked by microorganisms can be treated by experts by a variety of preventive interventions. Artistic medicine encompasses the monitoring of...
Article
Genome stability is continuously challenged by a wide range of DNA damaging factors. To promote a correct DNA repair and cell survival, cells orchestrate a coordinated and finely tuned cascade of events collectively known as the DNA damage Response (DDR). Ultra Violet (UV) rays are among the main environmental sources of DNA damage and a well recog...
Article
Full-text available
Chemotherapy-induced peripheral neuropathy (CIPN) is a type of neuropathic pain that represents a frequent and serious consequence of chemotherapy agents. Over the last years, significant progress has been achieved in elucidating the underlying pathogenesis of CIPN. The interference of taxanes with microtubule has been proposed as a mechanism that...
Chapter
Cell biology is a branch of biology focused on the study of cell structure and function, on how cells form and divide, and how they differentiate and specialize. Cell biology defines both the general properties, common to most cell types, and also dissects the unique features of specialized cells, which allow them to perform different functions.
Article
The objectives of the African Organization for Research and Training in Cancer (AORTIC), includes bringing products of decades of advances in cancer research to African populations through local and international collaboration. The consistent and huge growth in participation in the conferences and the diversity of the nations is a witness to the su...
Article
Full-text available
Obesity is characterized by a disruption in energy balance regulation that results in an excess accumulation of body fat. Its increasing prevalence poses a major public health concern because it is a risk factor for a host of additional chronic conditions, including type 2 diabetes, hypertension, and cardiovascular disease. Obesity is increasingly...
Article
Currently, there is much interest in the characterization of metabolic profiling of cancer stem cells (CSCs), a small subset of tumour cells with self-renewal capacity. Indeed, ever-growing evidence indicate that metabolism and stemness are highly intertwined processes in tumour tissue. In this review, we analyze the potential metabolic targeting s...
Article
Full-text available
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring au...
Article
CDK9 is a protein in constant development in cancer therapy. Herein we present an overview of the enzyme as a target for cancer therapy. We provide data on its characteristics and mechanism of action. In recent years, CDK9 inhibitors that have been designed with molecular modeling have demonstrated good antitumoral activity in vitro. Clinical studi...
Book
This text provides a comprehensive, state-of-the-art review of the application of image analysis focusing on the techniques which can be used in every biology and medical laboratory to automate procedures of cell analysis and to create statistics very useful for a comprehension of cell growth dynamics and the effects of drugs on them. This textbook...
Book
Facilitating the collaboration between the basic, translational, and clinical sciences, this book provides an overview of the genetic and epigenetic mechanisms underlying the formation and progression of gynecological cancers. Gynecological Cancers: Genetic and Epigenetic Targets and Drug Development gathers all of the molecular and cellular aspect...
Book
This text properly considers the most recent and relevant advances in molecular RB of GB, taking into account the related topics of pathobiology, and underscores the most promising translational perspectives from the preclinical to the clinical domain. Section I (From Bedside to Bench) discusses conditions associated with RT resistance of GB and th...
Article
Full-text available
The overexpression of ATP binding cassette (ABC) transporters makes tumor cells simultaneously resistant to several cytotoxic drugs. Impairing the energy metabolism of multidrug resistant (MDR) cells is a promising chemosensitizing strategy, but many metabolic modifiers are too toxic in vivo. We previously observed that the aminobisphosphonate zole...
Article
Full-text available
Giulio Bizzozero classified the tissues concerning their capacity to self-renew during the adult life in labile, stable and permanent tissues. In 1940 Viktor Hamburger and Rita Levi Montalcini exposed the possibility to induce the growth of permanent cells thanks to a specific ligand Nerve Growth Factor (NGF). Stanley Cohen purified a protein the E...
Article
While the nucleoporin 98-retinoic acid receptor gamma (NUP98-RARG) is the first RARG fusion protein found in acute leukemia, its roles and the molecular basis in oncogenic transformation are currently unknown. Here we showed that homodimeric NUP98-RARG not only acquired unique nuclear localization pattern and ability of recruiting both RXRA and wil...
Article
MicroRNAs (miRNAs) are important regulators of several cellular processes. During hematopoiesis, specific expression signatures have been reported in different blood cell lineages and stages of hematopoietic stem cell (HSC) differentiation. Here we explored the expression of miRNAs in umbilical cord blood stem (HSC) and progenitor cells (HPC) and c...
Article
Several investigators have cultivated marrow stromal cells and have identified a population of mesenchymal stem cells (MSCs). These cells expand extensively in vitro and exhibit multilineage differentiation potential. The lack of MSC-specific markers impedes identification of MSC functions. Further in vivo studies of these cells may elucidate the n...
Data
The p300-PSPD promotes p53 mutant aggregation. H1299 cell lines expressing p53G245A (panel A and B), or p53R175H (panel C) [57], were seeded on glass cover-slips and were transfected with RFP or with RFP-PSPD. After transfection cells were stained with the anti-p53 antibody (FL393). Note the nuclear pattern of localization of the p53 mutants in cel...
Data
Similarity between the p300 region comprised between amino acid 1688 to 1214 and various proteins containing prion-like domains in C. elegans (pqn and Abu family), and other species. All sequences found had a blast e-value above 0.001. (DOCX)
Data
Detection of p300 in cytoplasmic inclusions in the absence of MG132 treatment. A. Untreated or MG132 treated Cos-7 cells grown on coverslips were stained with anti-vimentin and anti-CBP (C22) antibodies. (TIF)
Data
The p300-PSPD brings TAU in cytoplasmic aggregates. A. Co-localization of endogenous p300 and TAU. Cos-7 cells were transfected with the vector expressing TAU and 24 hours after transfection cells were stained with the polyclonal antibody directed against p300 (C20, red, 1) and with a monoclonal antibody recognizing TAU (green, 2). In a parallel se...
Data
The p300-PSPD brings p53 and p21/WAF in cytoplasmic aggregates. A–D. H1299 cells expressing tetracycline-inducible p53 protein [56], were transfected with different p300 mutants previously described in Figure 3, specifically with the p300-Δ30 (A), p300-N (B) and p300-PSPD (C–D). Twenty four hours after induction of p53 via tetracycline addition, a...
Data
Dynamics of aggresomes in the presence of RFP-p300-PSPD. Cells were transfected with the vector expressing RFP-p300-PSPD, plated onto Lab-Tek II Chambered coverglasses System dish, and then treated with 5 µM MG132. The dishes were placed in a temperature-controlled stage at 37°C on an inverted microscope (Nikon Eclipse TE300) in a 5% CO2 atmosphere...
Data
A. H1299 cells were mock treated (A) or treated with 5 µM MG132 for 16 hours (BC) and cells were stained with a monoclonal mix of anti-ubiquitin, anti-p300, or anti-vimentin monoclonal as indicated in each panel. The arrows indicate the position of representative aggresomes. B. Human embryonic kidney carcinoma cells Hek293 cells were stained for p3...
Article
Full-text available
The current molecular targets in breast cancer (BC) clinical trials were identified before the advent of the genomic era and their relevance was confirmed and validated by the introduction of gene profiling. Pioneering molecular analyses and repeated data validations on different gene platforms have thus far served to define 5 subtypes of BC based...
Article
Full-text available
Prostate cancer represents the second leading cause of cancer deaths in Western countries. Viral infections could play a role in prostate carcinogenesis. Human polyomavirus BK (BKV) is a possible candidate because of its transforming properties. In this study, BKV sequences in urine, blood, fresh, and paraffin-embedded prostate cancer samples from...
Article
Intratubular germ cell neoplasia of unclassified type (IGCNU) is the precursor lesion of adult testicular germ cell invasive tumors. Primordial germ cells (PGCs) are recognized as the cells of origin of testicular germ cell tumors (TGCTs) because of the genetic and phenotypic characteristics analyzed. The most important risk factors responsible for...
Article
Cyclin D1 encodes a regulatory subunit, which with its cyclin-dependent kinase (Cdk)-binding partner forms a holoenzyme that phosphorylates and inactivates the retinoblastoma protein. In addition to its Cdk binding-dependent functions, cyclin D1 regulates cellular differentiation in part by modifying several transcription factors and nuclear recept...
Article
To define the role of virtual gastroscopy (VG) in the detection of elevated lesions comparing its results with conventional endoscopy (CE) as the gold standard method. Between December 2000 and July 2001, 19 patients were evaluated. The age ranged between 41 and 72 (mean 58.8 years old). VG and CE were performed in all the patients during the same...
Chapter
Lung cancer is one of the leading causes of cancer death in the world (1). The high mortality rate for lung cancer may be due to either or both the absence of standard clinical procedures for early diagnosis of tumors compared to breast, prostate, and colon cancers, and lack of effective treatments in more advanced stages of the disease (2).
Article
Although epidemiological findings have established that exposure to asbestos fibers is the major cause of malignant mesothelioma (MM), recent studies have implicated simian virus 40 (SV40) in the etiology of some of these tumors. Cytogenetic and molecular genetic evidence suggests that multiple somatic genetic events are required for tumorigenic co...
Article
Tat-mediated activation of the HIV-1 promoter activity requires Tat-dependent recruitment of the cyclinT1/CDK9 complex (P-TEFb) to the transacting element (TAR) RNA. Tat interaction with the cyclinT1, the regulatory partner of CDK9, results in a specific recruitment of the heterodimer CycT1/CDK9 complex to TAR, whereby it promotes transcription elo...
Article
Zinc-α 2-glycoprotein (Znα 2gp) is widely distributed in body fluids and epithelia. Its expression in stratified epithelia increases with differentiation. We previously showed that Zn α 2gp has ribonuclease activity, and that squamous tumor cells grown on a matrix of Znα 2gp were growth-inhibited. Here we demonstrate, both by adding Znα 2gp to the...
Article
Phosphorylation of the carboxyl-terminal domain (CTD) of RNA polymerase II (RNAPII) is an important step in transcription and the positive transcription elongation factor b (P-TEFb) has been proposed to facilitate elongation at many genes. The P-TEFb contains a catalytic subunit (Cdk9) that, in association with a cyclin subunit (cyclinT1), has the...
Article
Full-text available
The CDK9 kinase in association with Cyclin T is a component of the transcription positive-acting complex pTEFb which facilitates the transition from abortive to productive transcription elongation by phosphorylating the carboxyl-terminal domain of RNA polymerase II. The Cyclin T1/CDK9 complex is implicated in Tat transactivation, and it has been su...
Article
To examine the functional interaction between HIV-1 Tat protein and the cyclin T1 and T2 proteins which, in association with cyclin dependent kinase (CDK)9, are the regulatory subunits of the TAK/P-TEFb cellular complex strictly required for Tat transactivation. HIV-1 long terminal repeat (LTR) reporter plasmid was co-transfected into human and rod...
Article
A significant portion of published literature is dedicated to describing the cloning and the characterization of proteins involved in the progression of the cell cycle, which govern cell growth both in cancer and normal ontogenesis. With this abundance of information, the cascading pathways of molecular events that occur in the cell cycle are provi...
Article
Ovarian cancer is the fifth most common malignancy among American women and the fourth leading cause of cancer death. The rapid advances in molecular genetic analysis, presymptomatic detection, and treatment of ovarian cancer are staggering. In this review, both the genetic component and the molecular biology of ovarian cancer are discussed, as wel...
Article
Gene therapy has attracted much interest since the first submissions of phase I clinical trials in the early 1990s, for the treatment of inherited genetic diseases. Preliminary results were very encouraging and prompted many investigators to submit protocols for phase I and phase II clinical trials for the treatment of inherited genetic diseases an...
Article
HP infection is involved in the pathogenesis of several gastroduodenal diseases, as type B chronic gastritis, duodenal and gastric ulcer, MALT lymphoma and gastric cancer. The recent availability of molecular techniques, specifically the PCR, allow us to detect very low amounts of the bacterium. The aim of the study is to evaluate the presence of H...
Article
The proliferation of normal cells is regulated by a combination of stimulatory and inhibitory factors that can respond to external signals in a coordinated manner (MacLachlan et al. 1995; Grana and Reddy 1995; Paggi et al. 1996). Various mitogens, growth factors, cytokines, and a host of other agents can perturb the cell cycle machinery eliciting p...
Article
In eukaryotic cells, the coordinated activation of different cyclin-dependent kinases regulates entry into S-phase. In vitro and in nonproliferating cells, p27 associates with and inhibits cyclin/cycin-dependent kinase (CDK) holoenzymes containing either CDK4, CDK6, or CDK2. Although many different types of proliferating cells contain p27 protein,...
Chapter
Cancer is a genetic disease that results from multiple genomic changes. These ultimately lead to the deregulation of the cell cycle machinery and to autonomous cell proliferation. Neoplastic transformation involves four sets of genes: 1) oncogenes, 2) tumor-suppressor genes, 3) mutator genes, and 4) apoptotic genes. In the hematopoietic system, the...
Article
Human cyclin E, originally identified on the basis of its ability to function as a G1 cyclin in budding yeast, associated with a cell cycle-regulated protein kinase in human cells. The cyclin E-associated kinase activity peaked during G1, before the appearance of cyclin A, and was diminished during exit from the cell cycle after differentiation or...
Article
Several cellular proteins form stable complexes with the proteins encoded by the adenovirus early region 1A (E1A) gene in extracts derived from adenovirus infected or transformed cells. Two of the cellular proteins that bind to E1A have been identified; one, a 105-kilodalton protein (pRb), is the product of the retinoblastoma gene, and the other, a...
Article
The adenovirus early region 1A (E1A) proteins associate with several cellular proteins in adenovirus infected or transformed cells. Recently, two of the cellular proteins that bind to E1A encoded proteins have been identified. p105 has been shown to be the product of the retinoblastoma tumor suppressing gene. p60 has been shown to be a human cyclin...
Article
p60 is a cellular protein that binds to the adenovirus E1A protein complex in virally infected or transformed human cells. In both infected and uninfected cells, p60 was found in a complex with the cdc2 protein kinase. Immune complexes containing p60 and cdc2 display a cell cycle-dependent histone H1 kinase activity that is most active in interphas...

Projects

Project (1)
Project
Identification of novel proteins involved in the DNA damage response by using a synthetic DNA structure