Antonio Valle GallardoUniversidad de Cádiz | UCA · Department of Biomedicine, Biotechnology and Public Health
Antonio Valle Gallardo
Biologist
About
32
Publications
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199
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Citations since 2017
Introduction
Additional affiliations
December 2014 - December 2016
Publications
Publications (32)
Background
The human genome contains nearly 20.000 protein-coding genes, but there are still more than 6,000 proteins poorly characterized. Among them, ZNF330/NOA36 stand out because it is a highly evolutionarily conserved nucleolar zinc-finger protein found in the genome of ancient animal phyla like sponges or cnidarians, up to humans. Firstly des...
This study evaluated the feasibility of applying a microalgal peptide-enriched extract, obtained through a sequential alkaline and enzymatic method, as a fundamental media component in Escherichia coli culture. The results demonstrated that the extract is viable as the primary nutrient source for E. coli cultures (wild type), even as a complete sub...
Herbicides play a vital role in agriculture, contributing to increased crop productivity by minimizing weed growth, but their low degradability presents a threat to the environment and human health. Allelochemicals, such as DIBOA (2,4-dihydroxy-(2H)-1,4-benzoxazin-3(4H)-one), are secondary metabolites released by certain plants that affect the surv...
Introduction
Glycerol is a byproduct from the biodiesel industry that can be biotransformed by Escherichia coli to high added-value products such as succinate under aerobic conditions. The main genetic engineering strategies to achieve this aim involve the mutation of succinate dehydrogenase (sdhA) gene and also those responsible for acetate synthe...
During fermentation in Escherichia coli succinate is transported via Dcu transporters, encoded dcuA, dcuB, dcuC and dcuD although the role of DcuD protein has not been elucidated yet. It has been shown contribution of Dcu transporters in the N,N’-dicyclohexylcarbodiimide (DCCD) sensitive proton and potassium transport through the cytoplasmic membra...
The combustion of fossil fuels is leading to global warming due to the emissions of greenhouse gases. Hydrogen (H2) is a clean and sustainable energy source and a promising alternative to fossil fuels. The bacteria Escherichia coli naturally produces H2, and it has been widely modified by metabolic engineering to enhance this production. Malate pla...
The biotechnological production of dicarboxylic acids (C4) from renewable carbon sources represents an attractive approach for the provision of these valuable compounds by green chemistry means. Glycerol has become a waste product of the biodiesel industry that serves as a highly reduced carbon source for some microorganisms. Escherichia coli is ca...
Biotechnological production of chemicals and materials has received increasing attention as a substitute to petroleum-based refinery processes. Different microorganisms are used to this aim, although Escherichia coli stands out among them because it is the best-characterized bacterial species. The vast knowledge of its genome and metabolism has gen...
D-DIBOA (4-hydroxy-(2H)-1,4-benzoxazin-3-(4H)-one) is an allelopathic-derived compound with interesting herbicidal, fungicidal, and insecticide properties whose production has been successfully achieved by biocatalysis using a genetically engineered Escherichia coli strain. However, improvement and scaling-up of this process are hampered by the cur...
Anoxic biotrickling filters (BTFs) represent a technology with high H2S elimination capacity and removal efficiencies widely studied for biogas desulfurization. Three changes in the final electron acceptors were made using nitrate and nitrite during an operating period of 520 days. The stability and performance of the anoxic BTF were maintained whe...
Benzohydroxamic acids, such as DIBOA (2,4-dihydroxy-2 H)-1,4-benzoxazin-3(4 H)-one), are plant products that exhibit interesting herbicidal, fungicidal and bactericidal properties. A feasible alternative to their purification from natural sources is the synthesis of analogous compounds such as D-DIBOA (2-deoxy-DIBOA) and their chlorinated derivativ...
Background
The use of chemical herbicides has helped to improve agricultural production, although its intensive use has led to environmental damages. Plant allelochemicals are interesting alternatives due to their diversity and degradability in the environment. However, the main drawback of this option is their low natural production, which could b...
During fermentation Escherichia coli excrete succinate mainly via Dcu family carriers. Current work reveals the total and N,N’-dicyclohexylcarbodiimide (DCCD) inhibited ATPase activity at pH 7.5 and 5.5 in E. coli wild type and dcu mutants upon glycerol fermentation. The overall ATPase activity was highest at pH 7.5 in dcuABCD mutant. In wild type...
Hydrogen is a potential sustainable energy source and it could become an alternative to fossil fuel combustion, thus helping to reduce greenhouse gas emissions. The biological production of hydrogen, instead of its chemical synthesis, is a promising possibility since this process requires less energy and is more sustainable and eco-friendly. Severa...
Nitrate has been progressively replaced by nitrite as the electron acceptor in the oxidation of H2S from biogas mimic by an anoxic biotrickling filter. Perturbations in the inlet H2S concentration with and without the implementation of feedback controllers have been evaluated. Nitrite was successfully used without decreasing the H2S removal efficie...
Biological desulfurization has proven to be a process that is technically and economically feasible on using biotrickling filters that can be performed under aerobic and anoxic conditions. However, microbial communities are different mainly due to the use of different final electron acceptors. The analysis of microbial communities in these systems...
El empleo de microalgas en procesos integrados de depuración de gases ha cobrado un especial interés en los últimos años, debido principalmente a su capacidad para capturar CO2. Estas aplicaciones implican la generación de biomasa que puede utilizarse para obtener bioproductos y/o bioenergía, cuyo desarrollo requiere avanzar en métodos de ruptura c...
The production of biodiesel has emerged as an alternative to fossil fuels. However, this industry generates glycerol as a by-product in such large quantities that it has become an environmental problem. The biotransformation of this excess glycerol into other renewable bio-energy sources, like H2 and ethanol, by microorganisms such as Escherichia c...
Background
Earth’s climate is warming as a result of anthropogenic emissions of greenhouse gases from fossil fuel combustion. Bioenergy, which includes biodiesel, biohydrogen and bioethanol, has emerged as a sustainable alternative fuel source. For this reason, in recent years biodiesel production has become widespread but this industry currently g...
Biodiesel has emerged as an environmentally friendly alternative to fossil fuels; however, the low price of glycerol feed-stocks generated from the biodiesel industry has become a burden to this industry. A feasible alternative is the microbial biotransformation of waste glycerol to hydrogen and ethanol. Escherichia coli, a microorganism commonly u...
Benzohydroxamic acids, such as 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), exhibit interesting herbicidal, fungicidal and bactericidal properties. Recently, the chemical synthesis of D-DIBOA has been simplified to only two steps. In a previous paper, we demonstrated that the second step could be replaced by a biotransformation using Escheric...
Biotransformation of crude glycerol to ethanol by Escherichia coli, in a batch reactor
Process Biochemistry j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / p r o c b i o Biotransformation of ethyl 2-(2 -nitrophenoxy)acetate to benzohydroxamic acid (D-DIBOA) by Escherichia coli a b s t r a c t Benzohydroxamic acids, such as DIBOA, exhibit interesting biological properties (herbicidal, fungicidal and bac...
Genetic determinants for heavy metal resistance could be exploited in the design of bioprocesses for environmental cleanup. The removal of Ni(II), Co(II), Cr(VI) and Mn(II) was investigated using Serratia marcescens strains C-1, C4, 16 and Kluyvera sp. Nic3 isolated from nickel deposits in Moa, Holguín (Cuba). The high nickel and cobalt resistance...
Questions
Question (1)
I'm writing a revision about the electron transport chain in Escherichia coli and I know that the hydrogenases are involved in hydrogen production (Hyd 3) and hydrogen uptake (Hyd 1 and 2), but I don't understand clearly how hydrogen produced by Hyd 3 in the cytoplasm is transported across the inner membrane to the periplasmic space or is oxidized to protons and electrons by reverse functions of these enzymes.
