Antonio Bernabé-Antonio

University of Guadalajara | UDG · Departamento de Madera, Celulosa y Papel

Ageratina pichinchensis (Kunth) R.King & Ho.Rob. is a plant used in traditional Mexican medicine, and some biotechnological studies have shown that its calluses and cell suspension cultures can produce important anti-inflammatory compounds. In this study, we established a cell culture of A. pichinchensis in a 2 L airlift bioreactor and evaluated th...

Randia aculeata (Rubiaceae) is a medicinal plant distributed in the state of Jalisco, Mexico. In folk medicine it is used to counteract the effects produced by the bite of snakes and other poisonous insects. However, there are no reports of new alternatives to use the plant in a sustainable way. The main objective of this study was to establish a c...

Ageratina pichinchensis (Asteraceae) has been used for a long time in traditional Mexican medicine for treating different skin conditions and injuries. This review aimed to provide an up-to-date view regarding the traditional uses, chemical composition, and pharmacological properties (in vitro, in vivo, and clinical trials) that have been achieved...

Eysenhardtia platycarpa (Fabaceae) is a medicinal plant used in Mexico. Biotechnological studies of its use are lacking. The objective of this work was to establish a cell suspension culture (CSC) of E. platycarpa, determine the phytochemical constituents by spectrophotometric and gas chromatography‒mass spectrometry (GC‒MS) methods, evaluate its a...

Eysenhardtia platycarpa (Fabaceae) is a medicinal plant used in México and it lacks biotechnological studies for its use. The aim of this work was to establish a cell suspension cultures (CSC) of E. platycarpa, determine the phytochemical profile, and evaluate its antifungal activity. Friable callus and CSC were established with 2 mg/L 1-naphthalen...

Ageratina pichinchensis (Kunth) is a plant used in traditional Mexican medicine to treat multiple ailments. However, there have not been biotechnological studies on producing compounds in in vitro cultures. The aim of this study was to establish a cell suspension culture of A. pichinchensis, quantify the anti-inflammatory constituents 2,3-dihydrobe...

Shake-flask in vitro culture of Buddleja cordata cells produces large amounts of biomass and synthetizes verbascoside (VB), linarin and hydroxycinnamic acids, bioactive phenolic secondary metabolites (PSMs). In this work, we determined the effect of stirring speed on the growth of and production of PSMs [total phenolic, phenylethanoid glycoside and...

B. americana, B. cordata, B. parviflora, B. perfoliata, B. scordioides and B. sessiliflora, belonging to Buddleja Genus, are species distributed in Mexico reported in folk medicine to treat ills related to inflammatory processes. For some of them, this ethnopharmacological uses have been validated by scientific studies. Their phytochemistry has bee...

In the present work reports the establishment of a cell suspension culture (CSC) from C. brasiliense. In addition, extracts were obtained from the CSC dry biomass and leaves of wild plant using organic solvents for the purpose of determining the total phenolic (FET) and total flavonoid (FLT) content. Furthermore, it was evaluated the antioxidant an...

Cnidoscolus chayamansa ('Tree spinach' or Chaya) produces lupeol acetate (LUAC) naturally. Cell culture may represent a viable alternative production method to sustainably use of natural resources. No studies have reported LUAC production in vitro or in Chaya cell culture. The aim of the study was to establish a C. chayamansa cell suspension cultur...

La teca es una especie importante en los mercados forestales, debido al valor de su madera, lo cual en México ha incrementado el interés de establecer plantaciones. Sin embargo, la influencia de las propiedades del suelo y nitrógeno bajo esas condiciones han sido poco evaluadas. El objetivo del presente estudio fue medir la calidad del suelo en pla...

The tolerance index (TI) and the bioaccumulation factor (BF) for the estimation of accumulation and tolerance of different heavy metals in cell suspension cultures are reviewed. Procedures for measuring these parameters are described for the purposes of phytoremediation research.

A protocol was established to produce bioactive compounds in a callus culture of Ageratina pichinchensis by using 1 mg L−1 NAA with 0.1 mg L−1 KIN. The phytochemical study of the EtOAc extract obtained from the callus biomass, allowed the isolation and characterization of eleven secondary metabolites, of which dihydrobenzofuran (5) and 3-epilupeol...

A protocol was established to produce bioactive compounds in a callus culture of Ageratina pichinchensis by using 1 mg L⁻¹ NAA with 0.1 mg L⁻¹ KIN. The phytochemical study of the EtOAc extract obtained from the callus biomass, allowed the isolation and characterization of eleven secondary metabolites, of which dihydrobenzofuran (5) and 3-epilupeol...

Cnidoscolus chayamansa is a plant widely used as food with potential medicinal applications. Plant tissue culture enables the production of secondary metabolites from plants in a sustainable manner. The aim of this work was to establish a callus culture of C. chayamansa using leaf explants, which, in the future, can be used to obtain whole extracts...

Dada su riqueza forestal, México cuenta con numerosas especies silvestres del género Physalis (Solanaceae) que poseen potencial para su aprovechamiento, desde el punto de vista fitoquímico; además tiene importantes propiedades medicinales. Physalis chenopodifolia es una de ellas. Taxon de amplia presencia en el territorio nacional, pero poco estudi...

Edited by AO Aremu Eysenhardtia polystachya (Fabaceae) is a valuable medicinal plant from Mexico. There have been no cell culture studies of this plant to produce secondary metabolites. The aim of this work was to establish callus and cell suspension cultures of E. polystachya (Ortega) and to evaluate the content of phenols, total flavonoids, and f...

Background Calophyllum brasiliense is highlighted as an important resource of calanolides, which are dipyranocoumarins that inhibit the reverse transcriptase of human immunodeficiency virus type 1 (HIV-1 RT). Despite having great medicinal importance, enzymes involved in calanolide, biosynthesis and the pathway itself, are still largely unknown. Ad...

Buddleja cordata is a medicinal plant distributed in Mexican territory that is characterized for producing phenolic compounds possessing antioxidant activity. It was evaluated the type of morphogenetic responses induced by plant growth regulators. Furthermore, the content of verbascoside and total phenols was determined, as well as the antioxidant...

Jatropha curcas is a tolerant and accumulator plant of heavy metals (HMs). Little is known about the mechanisms behind this ability. It is suggested that antioxidant enzymes might participate; however, there are no studies reporting the relationship between the activities of antioxidant enzymes and the presence of HMs in an in vitro cell suspension...

Calophyllum brasiliense (Calophyllaceae) is a medicinal tree known mainly for producing calanolides, secondary metabolites against HIV-1 reverse transcriptase. This wild plant is listed as threatened and despite its outstanding medicinal value, no studies have been conducted on its propagation or preservation. This study standardized a procedure fo...

Due to its abundant forestry resources, Mexico has numerous species of the Physalis genus (Solanaceae), plants with a phytochemical potential and important medicinal properties. Physalis chenopodifolia, one of these Solanaceae with a widespread presence in our country, has been little studied. Therefore, the purpose of this work was the mineral cha...

Jatropha curcas has shown to be a bioaccumulator and a tolerant species to different heavy metals (HMs). This study presents an evaluation system for tolerance and accumulation of chromium (Cr) and lead (Pb) in J. curcas using a cell suspension culture (CSC). This CSC was established with callus obtained from hypocotyls segments, onto Murashige-Sko...

A great diversity of medicinal plants have been traditionally used against gastropathy, infections and inflammatory pathological ailments. C. brasiliense (Clusiaceae) is a large tree native to the tropical forest which thrives from Brazil to Mexico. It is a plant commonly used in traditional medicine against several diseases, infections and other p...

In vitro studies indicate that Prosopis laevigata can be considered a potential hyperaccumulator of lead. Likewise, lead uptake has been related to protein transporters for zinc. In this work presents a protocol for the establishment of cell suspension culture to determine the effect of zinc on the uptake and accumulation of lead. A bioassay with P...

Dioscorea remotiflora (Kunth) is an important wild plant that produces tuberous roots used as a source of food in the Western part of Mexico. Lack of planting material and inefficiency of traditional methods of propa-gation are the main constraints for implementing large-scale cultivation. In contrast, tissue culture techniques allow increasing mul...

Calophyllum brasiliense (Cambes) produces calanolide secondary metabolites that are active against human immunodeficiency virus type 1 reverse transcriptase. In this study, it was demonstrated that plant tissue culture is a useful technique for producing these metabolites. Different concentrations and combinations of plant growth regulators were te...