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Antonella Bongiovanni

Antonella Bongiovanni
Italian National Research Council (CNR) - Italy · Institute of Research and Innovation (IRIB), Consiglio Nazionale delle Ricerche (CNR), 90146 Palermo, Italy

PhD

About

92
Publications
25,295
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8,185
Citations
Additional affiliations
December 2001 - June 2015
Italian National Research Council
Position
  • Researcher
December 2001 - July 2017
Italian National Research Council (CNR) - Italy
Position
  • Principal Investigator

Publications

Publications (92)
Article
Several cell types secrete small membranous vesicles that contain cell-specific collections of proteins, lipids, and genetic material. The function of these vesicles is to allow cell-to-cell signaling and the horizontal transfer of their cargo molecules. Here, we demonstrate that muscle cells secrete nano-sized vesicles and that their release incre...
Article
The sea urchin provides a relatively simple and tractable system for analyzing the early stages of embryo development. Here, we use the sea urchin species, Paracentrotus lividus, to investigate the role of Alix in key stages of embryogenesis, namely the egg fertilization and the first cleavage division. Alix is a multifunctional protein involved in...
Article
Full-text available
Alix/AIP1 is a multifunctional adaptor protein that participates in basic cellular processes, including membrane trafficking and actin cytoskeleton assembly, by binding selectively to a variety of partner proteins. However, the mechanisms regulating Alix turnover, subcellular distribution, and function in muscle cells are unknown. We now report tha...
Article
Full-text available
Muscle contractile proteins are expressed as a series of developmental isoforms that are in constant dynamic remodeling during embryogenesis, but how obsolete molecules are recognized and removed is not known. Ozz is a developmentally regulated protein that functions as the adaptor component of a RING-type ubiquitin ligase complex specific to stria...
Article
The identities of the ubiquitin-ligases active during myogenesis are largely unknown. Here we describe a RING-type E3 ligase complex specified by the adaptor protein, Ozz, a novel SOCS protein that is developmentally regulated and expressed exclusively in striated muscle. In mice, the absence of Ozz results in overt maturation defects of the sarcom...
Article
Alix is a ubiquitously expressed scaffold protein that participates in numerous cellular processes related to the remodeling/repair of membranes and the actin cytoskeleton. Alix exists in monomeric and dimeric/multimeric configurations, but how dimer formation occurs and what role the dimer has in Alix-mediated processes are still largely elusive....
Article
Full-text available
Tumor growth and metastasis strongly rely on cell–cell communication. One of the mechanisms by which tumor cells communicate involves the release and uptake of lipid membrane encapsulat-ed particles full of bioactive molecules, called extracellular vesicles (EVs). EV exchange between cancer cells may induce phenotype changes in the recipient cells....
Article
Full-text available
Extracellular vesicles (EVs) play a crucial role as potent signal transducers among cells, with the potential to operate cross-species and cross-kingdom communication. Nanoalgosomes are a subtype of EVs recently identified and isolated from microalgae. Microalgae represent a natural bioresource with the capacity to produce several secondary metabol...
Article
Full-text available
Extracellular vesicles (EVs) are lipid membrane nano-sized vesicles secreted by various cell types for intercellular communication, found in all kingdoms of life. Nanoalgosomes are a subtype of EVs derived from microalgae with a sustainable biotechnological potential. To explore the uptake, distribution and persistence of nanoalgosomes in cells and...
Chapter
Metaphors represent a powerful cognitive tool through which it is possible to facilitate the understanding of abstract concepts or concepts belonging to unfamiliar domains. In the field of Serious Games (SGs), metaphors connect the user’s previous experience in the game domain to the information load that is intended to be conveyed. This paper pres...
Article
Neurodegenerative diseases affect millions of people worldwide and the presence of various physiological barriers limits the accessibility to the brain and reduces the efficacy of various therapies. Moreover, new carriers having targeting properties to specific brain regions and cells are needed in order to improve therapies for the brain disorder...
Data
Supplementary Figure 2. Comparison of the factor space explored by the full factorial (FF), Box-Behnken (BB), and central composite (CC) designs.
Data
Supplementary Figure 3. Central composite design. (A) Worksheet with all the combinations tested and the relative output. (B) On the left, the normal probability plot showing a normal residual distribution. On the right, the residual versus fits plot showing the constant variance of the residuals.
Data
Supplementary Figure 1. Box-Behnken design. (A) Worksheet with all the combinations analyzed and the relative output. (B) On the left, the normal probability plot showing a normal residual distribution. On the right, the residual versus fits plot showing the constant variance of the residuals.
Data
Supplementary Table Worksheet of the screening two-level full factorial design with all the combinations to test.
Article
Full-text available
Transfection is the process by which nucleic acids are introduced into eukaryotic cells. This is fundamental in basic research for studying gene function and modulation of gene expression as well as for many bioprocesses in the manufacturing of clinical-grade recombinant biologics from cells. Transfection efficiency is a critical parameter to incre...
Article
Full-text available
Extracellular vesicles (EVs) are emerging as promising diagnostic and therapeutic tools for a variety of diseases. The characterization of EVs requires a series of orthogonal techniques that are overall time‐ and material‐consuming. Here, a microfluidic device is presented that exploits the combination of diffusion sizing and multiwavelength fluore...
Article
Full-text available
The 2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) is one of the most prominent PBDE congeners detected in the environment and in animal and human tissues. Animal model experiments suggested the occurrence of PBDE-induced immunotoxicity leading to different outcomes and recently we demonstrated that this substance can impair macrophage and basophil ac...
Article
Full-text available
Microalgae constitute a heterogeneous and diverse range of organisms capable of accumulating bioactive metabolites, making them promising feedstock for applications in the nutraceutical, functional food, animal feed, biofertilisation or biofuel sectors. There has been renewed interest in recent times in natural sources of antioxidants, particularly...
Article
Full-text available
Cellular, inter‐organismal and cross kingdom communication via extracellular vesicles (EVs) is intensively studied in basic science with high expectation for a large variety of bio‐technological applications. EVs intrinsically possess many attributes of a drug delivery vehicle. Beyond the implications for basic cell biology, academic and industrial...
Article
Full-text available
Individuals infected with the severe acute respiratory syndrome (SARS)‐related coronavirus 2 (SARS‐CoV‐2), develop a critical and even fatal disease, called Coronavirus disease‐19 (COVID‐19), that eventually evolves into acute respiratory distress syndrome. The gravity of the SARS‐CoV‐2 pandemic, the escalating number of confirmed cases around the...
Article
Full-text available
Nowadays, Quality Management tools such as failure mode and effect analysis (FMEA) are widely used throughout the aeronautical, automotive, software, food services, health care and many other industries to sustain and improve quality and safety. The increasing complexity of scientific research makes it more difficult to maintain all activities unde...
Article
We show that extracellular vesicles (EVs) released by mesenchymal cells (i.e., fibro-adipogenic progenitors-FAPs) mediate microRNA (miR) transfer to muscle stem cells (MuSCs) and that exposure of dystrophic FAPs to HDAC inhibitors (HDACis) increases the intra-EV levels of a subset of miRs, which cooperatively target biological processes of therapeu...
Chapter
Extracellular vesicles (EVs) are phospholipid bilayer enclosed structures containing specific proteins, nucleic acids, and metabolites, which are naturally released from cells as vehicles of intercellular communication. Post-translational modifications (PTMs) of EV proteins can regulate EVs biogenesis or play diverse roles in their biology, in both...
Conference Paper
Full-text available
In this study, we investigated toxic effects of an anionic surfactant sodium dodecyl sulphate (SDS), and a non-ionic surfactant Triton X-100 (TX100) on a unicellular marine microalgae. Loss of motility was observed as one of the primary effects of both surfactants, with effective concentrations of 0.02 pmol/cell (16 µM) for TX100, and 0.35 pmol/cel...
Preprint
Full-text available
Functional interactions between cellular components of the muscle stem cell (MuSC) niche regulate the regenerative ability of skeletal muscles in physiological and pathological conditions; however, the identity of the mediators of these interactions remains largely unknown. We show here that fibro-adipogenic progenitor (FAP)-derived Extracellular V...
Article
The cover represents one of the most urgent needs in the field of extracellular vesicles: a scalable and standardized process to produce vesicles with high yield and reproducible qualities. In article 1800528. Carolina Paganini, Umberto Capasso Palmiero, Gabriella Pocsfalvi, Nicolas Touzet, Antonella Bongiovanni, and Paolo Arosio summarise the stat...
Article
Full-text available
Background: miRNAs are master regulators of signalling pathways critically involved in asthma and are transferred between cells in extracellular vesicles (EV). We aimed to investigate if the miRNA content of EV secreted by primary normal human bronchial epithelial cells (NHBE) is altered upon asthma development. Methods: NHBE cells were cultured...
Article
Full-text available
Lysosomal exocytosis is a ubiquitous process negatively regulated by neuraminidase 1 (NEU1), a sialidase mutated in the glycoprotein storage disease sialidosis. In Neu1 −/− mice, excessive lysosomal exocytosis is at the basis of disease pathogenesis. Yet, the tissue-specific molecular consequences of this deregulated pathway are still unfolding. We...
Article
Full-text available
Potential applications of extracellular vesicles (EVs) are attracting increasing interest in the fields of medicine, cosmetics and nutrition. However, manufacturing of EVs is currently characterized by low yields. This limitation severely hampers progress in research at the laboratory and clinical scale, as well as the realization of successful and...
Article
Full-text available
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many...
Article
Full-text available
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many...
Article
Background: Virtually all cell types have the capacity to secrete nanometer-sized extracellular vesicles, which have emerged in recent years as potent signal transducers and cell-cell communicators. The multifunctional protein Alix is a bona fide exosomal regulator and skeletal muscle cells can release Alix-positive nano-sized extracellular vesicl...
Article
Full-text available
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many...
Preprint
Full-text available
Quality disciplines have been widely used for decades in industrial and business fields. It is only in recent times, however, that Quality management and approaches have received proper attention in life science. The development, optimization, validation and dissemination of an innovative way of planning and organizing research activity, inspired b...
Preprint
Full-text available
Quality disciplines have been widely used for decades in industrial and business fields. It is only in recent times, however, that Quality management and approaches have received proper attention in life science. The development, optimization, validation and dissemination of an innovative way of planning and organizing research activity, inspired b...
Article
Full-text available
Cell surface expression of alpha-enolase, a glycolytic enzyme displaying moonlighting activities, has been shown to contribute to the motility and invasiveness of cancer cells through the protein non-enzymatic function of binding plasminogen and enhancing plasmin formation. Although a few recent records indicate the involvement of protein partners...
Article
Epithelial-mesenchymal transition (EMT) is an evolutionarily conserved cellular program, which is a prerequisite for the metastatic cascade in carcinoma progression. Here, we evaluate the EMT process using the sea urchin Paracentrotus lividus embryo. In sea urchin embryos, the earliest EMT event is related to the acquisition of a mesenchymal phenot...
Preprint
Full-text available
Quality disciplines have been widely used for decades in industrial and business fields. It is only in recent times, however, that Quality management and approaches have received proper attention in life science. In particular, the need for Quality standards in non-regulated research is a matter of considerable current debate inside international r...
Preprint
Full-text available
Quality disciplines have been widely used for decades in industrial and business fields. It is only in recent times, however, that Quality management and approaches have received proper attention in life science. In particular, the need for Quality standards in non-regulated research is a matter of considerable current debate inside international r...
Article
Maintenance of epithelial cell polarity and epithelial barrier relies on the spatial organization of the actin cytoskeleton and proper positioning/assembly of intercellular junctions. However, how these processes are regulated is poorly understood. Here we reveal a key role for the multifunctional protein Alix in both processes. In a knockout mouse...
Data
3D reconstruction of scanning laser confocal microscopy images of WT choroid plexus stained for CD9.
Data
3D reconstruction of whole-mount scanning electron microscopy (SEM) images showed the organization and orientation of cilia of the lateral wall in WT control.
Data
Cilia beating of ependymal cells from WT mice. Micro-video recording of lateral wall shows the beating motion of cilia in WT control.
Data
3D-Reconstructed volume of whole-mount confocal microscopy images of WT choroid plexus stained with phalloidin.
Data
3D-confocal reconstruction of WT whole-mount CP showing the expression pattern of ZO-1
Data
3D-confocal reconstruction of Alix-/- whole-mount CP showing the expression pattern of ZO-1
Data
3D reconstructed whole-mount scanning electron microscopy (SEM) images showed the organization of the lateral CP in WT.
Data
3D reconstructed whole-mount scanning electron microscopy (SEM) images showed the organization of the lateral CP in Alix-/- mice.
Data
The speed of ependymal flow was significantly reduced in Alix-/- mice. The speeds of fluorescent beads on the brain coronal section preparation were slower and stagnate in Alix-/- mice compare to WT control.
Data
3-D reconstruction of scanning laser confocal microscopy Z-stacks of WT choroid plexus immunostained for Occludin1.
Data
Micro-video showing the ependymal flow in WT lateral wall.
Data
3D-Reconstructed volume of whole-mount confocal microscopy images of Alix-/- choroid plexus stained with phalloidin showed the organization of the CP compared to WT.
Data
3D reconstruction of scanning laser confocal microscopy images of Alix-/- choroid plexus stained for CD9.
Data
3D reconstruction of whole-mount scanning electron microscopy (SEM) images showed the organization and orientation of cilia of the lateral wall in Alix-/-. The Alix-/- cilia are of different sizes and are also highly disorganized.
Data
Cilia beating of ependymal cells from Alix-/- mice. The Alix-/- motile cilia display an asynchronous beating.
Data
3-D reconstruction of scanning laser confocal microscopy Z-stacks of Alix-/- choroid plexus immunostained for Occludin1.
Article
Nowadays, the requirement to define adequate standards and to identify and validate general guidelines for scientific activity is becoming increasingly apparent also in non-regulated scientific research. Guidelines are fundamental tools to provide valid indications for proper conduct in a research laboratory, the correct use of equipment and proced...
Article
Full-text available
Quality principles and Project Management (PM) methodologies have long been ignored in non-regulated scientific research, even though they have been widely used in industrial and business applications, improving management and results and reducing costs. A groundbreaking project named Quality and Project Management OpenLab was implemented by a netw...
Chapter
Design of Experiments (DoE) statistical methodology permits the simultaneous evaluation of the effects of different factors on experimental performance and the analysis of their interactions in order to identify their optimal combinations. Compared to classical approaches based on changing only one factor at a time (OFAT), DoE facilitates the explo...
Article
Full-text available
Motivation: Extracellular vesicles are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of the vesicular nomenclatures and components, online searches for extracellular vesicle-related publications and vesicular components are currently challenging. Results: We present an improved version of EVpedia, a...